(Circular) (Six-base) MAPSORT of: pFN21AB5970.seq Check: 3070 from: 1 to: 6385 pFN21AB5970 6385 bp With 182 enzymes: SgfI * August 16, 2010 14:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4195 278 Size: 53 83 186 936 2659 2468 Fragments arranged by size: 2659 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3411 3728 1937 Size: 1474 317 4594 Fragments arranged by size: 4594 1474 317 Acc65I G'GTAC_C Cuts at: 3706 3706 Size: 6385 AclI AA'CG_TT Cuts at: 1563 4512 4885 1563 Size: 2949 373 3063 Fragments arranged by size: 3063 2949 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3308 3557 4448 5832 6100 1632 Size: 1676 249 891 1384 268 1917 Fragments arranged by size: 1917 1676 1384 891 268 249 AfeI AGC'GCT Cuts at: 3276 3428 3276 Size: 152 6233 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6188 AflIII A'CryG_T Cuts at: 1203 5284 6261 1203 Size: 4081 977 1327 Fragments arranged by size: 4081 1327 977 AgeI A'CCGG_T Cuts at: 2318 5216 2318 Size: 2898 3487 AhdI GACnn_n'nnGTC Cuts at: 3103 5114 3103 Size: 2011 4374 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6385 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6385 AlwNI CAG_nnn'CTG Cuts at: 2392 2701 3304 4128 5700 2392 Size: 309 603 824 1572 3077 Fragments arranged by size: 3077 1572 824 603 309 ApaI G_GGCC'C Cuts at: 2275 2285 2275 Size: 10 6375 ApaLI G'TGCA_C Cuts at: 4442 5598 4442 Size: 1156 5229 ApoI r'AATT_y Cuts at: 1493 3694 3920 4072 6181 1493 Size: 2201 226 152 2109 1697 Fragments arranged by size: 2201 2109 1697 226 152 AscI GG'CGCG_CC Cuts at: 3131 3218 3566 3131 Size: 87 348 5950 Fragments arranged by size: 5950 348 87 AseI AT'TA_AT Cuts at: 160 1032 4939 160 Size: 872 3907 1606 Fragments arranged by size: 3907 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6385 AvaI C'yCGr_G Cuts at: 1870 1943 2286 2574 2843 2922 3184 3443 3710 Size: 73 343 288 269 79 262 259 267 Cuts at: 3710 1870 Size: 4545 Fragments arranged by size: 4545 343 288 269 267 262 259 79 73 BamHI G'GATC_C Cuts at: 2950 3079 3328 3715 4082 2950 Size: 129 249 387 367 5253 Fragments arranged by size: 5253 387 367 249 129 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2102 2552 3109 3706 5161 Size: 325 217 627 315 450 557 597 1455 Cuts at: 5161 618 Size: 1842 Fragments arranged by size: 1842 1455 627 597 557 450 325 315 217 BanII G_rGCy'C Cuts at: 1391 2059 2230 2275 2285 2457 2775 3405 3704 Size: 668 171 45 10 172 318 630 299 Cuts at: 3704 1391 Size: 4072 Fragments arranged by size: 4072 668 630 318 299 172 171 45 10 BbeI G_GCGC'C Cuts at: 3113 3113 Size: 6385 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2133 928 Size: 971 234 5180 Fragments arranged by size: 5180 971 234 BbvCI CC'TCA_GC Cuts at: 2441 3053 2441 Size: 612 5773 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4576 4610 1903 Size: 34 50 34 2555 34 3678 Fragments arranged by size: 3678 2555 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4277 5487 4277 Size: 1210 5175 BclI T'GATC_A Cuts at: 1548 1548 Size: 6385 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2054 2827 3243 Size: 107 122 71 631 605 382 773 416 Cuts at: 3243 3252 3554 3563 4996 136 Size: 9 302 9 1433 1525 Fragments arranged by size: 1525 1433 773 631 605 416 382 302 122 107 71 9 9 BglII A'GATC_T Cuts at: 6380 6380 Size: 6385 BlpI GC'TnA_GC Cuts at: 3404 3802 3404 Size: 398 5987 Bme1580I G_kGCm'C Cuts at: 1790 2105 2275 2285 2557 2814 3352 3588 3654 Size: 315 170 10 272 257 538 236 66 Cuts at: 3654 4446 5602 1790 Size: 792 1156 2573 Fragments arranged by size: 2573 1156 792 538 315 272 257 236 170 66 10 BmgBI CAC'GTC Cuts at: 1318 3105 1318 Size: 1787 4598 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2189 4116 5074 Size: 416 53 83 399 24 772 1927 958 Cuts at: 5074 442 Size: 1753 Fragments arranged by size: 1927 1753 958 772 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6385 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2127 2429 2484 2897 3032 5045 1239 Size: 888 302 55 413 135 2013 2579 Fragments arranged by size: 2579 2013 888 413 302 135 55 Bpu10I CC'TnA_GC Cuts at: 2441 3053 2441 Size: 612 5773 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4510 5375 5673 4510 Size: 865 298 5222 Fragments arranged by size: 5222 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2283 3440 5048 882 Size: 617 784 1157 1608 2219 Fragments arranged by size: 2219 1608 1157 784 617 BsaAI yAC'GTr Cuts at: 493 1204 3523 3649 4176 493 Size: 711 2319 126 527 2702 Fragments arranged by size: 2702 2319 711 527 126 BsaBI GATnn'nnATC Cuts at: 6379 6379 Size: 6385 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3110 4192 4574 5209 Size: 53 83 186 936 1577 1082 382 635 Cuts at: 5209 275 Size: 1451 Fragments arranged by size: 1577 1451 1082 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2318 2953 4818 5216 5490 5637 6048 2318 Size: 635 1865 398 274 147 411 2655 Fragments arranged by size: 2655 1865 635 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6160 Fragments arranged by size: 6160 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2102 2481 2861 3047 3641 1186 Size: 916 379 380 186 594 3930 Fragments arranged by size: 3930 916 594 380 379 186 BseYI C'CCAG_C Cuts at: 2062 2277 2356 2540 2858 5588 6084 2062 Size: 215 79 184 318 2730 496 2363 Fragments arranged by size: 2730 2363 496 318 215 184 79 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2285 3077 1163 Size: 573 549 792 4471 Fragments arranged by size: 4471 792 573 549 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3499 3835 4596 5624 1540 Size: 275 186 1498 336 761 1028 2301 Fragments arranged by size: 2301 1498 1028 761 336 275 186 BsiHKAI G_wGCw'C Cuts at: 3405 4446 4531 5602 3405 Size: 1041 85 1071 4188 Fragments arranged by size: 4188 1071 1041 85 BsmI GAATG_Cn' Cuts at: 3896 3989 3896 Size: 93 6292 BsmBI CGTCTCn'nnnn_ Cuts at: 2873 2873 Size: 6385 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2059 2105 2230 2275 2285 2457 2557 Size: 399 269 46 125 45 10 172 100 Cuts at: 2557 2775 2814 3352 3405 3588 3654 3704 4446 Size: 218 39 538 53 183 66 50 742 Cuts at: 4446 4531 5602 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 538 399 269 218 183 172 125 100 85 66 53 50 46 45 39 10 BspEI T'CCGG_A Cuts at: 2953 6048 2953 Size: 3095 3290 BspHI T'CATG_A Cuts at: 4274 6004 4274 Size: 1730 4655 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2022 3740 844 Size: 1178 1718 3489 Fragments arranged by size: 3489 1718 1178 BsrBI CCG'CTC Cuts at: 1394 1823 4272 1394 Size: 429 2449 3507 Fragments arranged by size: 3507 2449 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2200 4880 5054 65 Size: 1183 952 2680 174 1396 Fragments arranged by size: 2680 1396 1183 952 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2318 2966 5029 5216 1700 Size: 110 508 648 2063 187 2869 Fragments arranged by size: 2869 2063 648 508 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6385 BssHII G'CGCG_C Cuts at: 1925 3131 3218 3390 3566 3639 1925 Size: 1206 87 172 176 73 4671 Fragments arranged by size: 4671 1206 176 172 87 73 BssSI C'ACGA_G Cuts at: 4445 5457 4445 Size: 1012 5373 BstAPI GCAn_nnn'nTGC Cuts at: 794 2701 3304 3649 794 Size: 1907 603 345 3530 Fragments arranged by size: 3530 1907 603 345 BstBI TT'CG_AA Cuts at: 5189 5189 Size: 6385 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6385 BstXI CCAn_nnnn'nTGG Cuts at: 3127 3661 3127 Size: 534 5851 BstYI r'GATC_y Cuts at: 1972 2950 3079 3328 3715 4082 4467 4484 5936 Size: 978 129 249 387 367 385 17 1452 Cuts at: 5936 6022 6034 6380 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 978 387 385 367 346 249 129 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2520 2520 Size: 6385 BtgI C'CryG_G Cuts at: 513 1065 2659 2762 2835 3003 3113 3120 3550 Size: 552 1594 103 73 168 110 7 430 Cuts at: 3550 513 Size: 3348 Fragments arranged by size: 3348 1594 552 430 168 110 103 73 7 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5646 Fragments arranged by size: 5646 683 56 BtsI GCAGTG_nn' Cuts at: 2699 3060 3901 4695 4715 2699 Size: 361 841 794 20 4369 Fragments arranged by size: 4369 841 794 361 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6385 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6350 DraI TTT'AAA Cuts at: 3689 3689 Size: 6385 DraIII CAC_nnn'GTG Cuts at: 3526 3649 3672 3526 Size: 123 23 6239 Fragments arranged by size: 6239 123 23 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5392 809 Size: 507 4076 1802 Fragments arranged by size: 4076 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2295 2794 2964 Size: 54 1425 50 275 18 465 499 170 Cuts at: 2964 3066 3198 3663 3832 4721 6350 8 Size: 102 132 465 169 889 1629 43 Fragments arranged by size: 1629 1425 889 499 465 465 275 170 169 132 102 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3832 1537 Size: 275 2020 4090 Fragments arranged by size: 4090 2020 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4315 1353 Size: 2962 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4972 5346 5492 387 Size: 1064 138 3383 374 146 1280 Fragments arranged by size: 3383 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3403 3403 Size: 6385 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2127 2429 2484 2897 3032 3308 3557 Size: 393 495 302 55 413 135 276 249 Cuts at: 3557 4448 5045 5832 6100 1239 Size: 891 597 787 268 1524 Fragments arranged by size: 1524 891 787 597 495 413 393 302 276 268 249 135 55 EcoNI CCTnn'n_nnAGG Cuts at: 2440 3623 2440 Size: 1183 5202 EcoO109I rG'GnC_Cy Cuts at: 2011 2074 2271 2281 2282 2361 2374 2619 2911 Size: 63 197 10 1 79 13 245 292 Cuts at: 2911 2980 3245 3301 2011 Size: 69 265 56 5095 Fragments arranged by size: 5095 292 265 245 197 79 69 63 56 13 10 1 EcoRI G'AATT_C Cuts at: 3694 4072 3694 Size: 378 6007 FspI TGC'GCA Cuts at: 2790 4891 2790 Size: 2101 4284 HaeII r_GCGC'y Cuts at: 1678 2238 2392 2492 2906 3011 3019 3113 3278 Size: 560 154 100 414 105 8 94 165 Cuts at: 3278 3296 3430 5532 1678 Size: 18 134 2102 2531 Fragments arranged by size: 2531 2102 560 414 165 154 134 105 100 94 18 8 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3089 3121 5026 5058 5100 Size: 32 135 32 1517 32 1905 32 42 Cuts at: 5100 5132 1373 Size: 32 2626 Fragments arranged by size: 2626 1905 1517 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2989 3729 3975 669 Size: 988 281 1051 740 246 3079 Fragments arranged by size: 3079 1051 988 740 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6385 HpaI GTT'AAC Cuts at: 3975 3975 Size: 6385 KasI G'GCGC_C Cuts at: 3109 3109 Size: 6385 KpnI G_GTAC'C Cuts at: 3710 3710 Size: 6385 MfeI C'AATT_G Cuts at: 3463 3984 3463 Size: 521 5864 MluI A'CGCG_T Cuts at: 6261 6261 Size: 6385 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2976 3519 3603 5499 5683 1305 Size: 1671 543 84 1896 184 2007 Fragments arranged by size: 2007 1896 1671 543 184 84 MscI TGG'CCA Cuts at: 10 64 1489 1832 2297 3665 10 Size: 54 1425 343 465 1368 2730 Fragments arranged by size: 2730 1425 1368 465 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2735 4343 4702 4861 518 Size: 648 1569 1608 359 159 2042 Fragments arranged by size: 2042 1608 1569 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2096 2277 2661 2724 2764 2782 2800 2837 Size: 490 181 384 63 40 18 18 37 Cuts at: 2837 3005 3040 3211 3801 4478 5626 5871 1606 Size: 168 35 171 590 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 590 490 384 245 181 171 168 63 40 37 35 18 18 NaeI GCC'GGC Cuts at: 2968 2968 Size: 6385 NarI GG'CG_CC Cuts at: 3110 3110 Size: 6385 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5833 NdeI CA'TA_TG Cuts at: 387 387 Size: 6385 NgoMIV G'CCGG_C Cuts at: 2966 2966 Size: 6385 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6385 NotI GC'GGCC_GC Cuts at: 3832 3832 Size: 6385 NspI r_CATG'y Cuts at: 1132 1229 3743 5288 1132 Size: 97 2514 1545 2229 Fragments arranged by size: 2514 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2050 2378 2507 1348 Size: 702 328 129 5226 Fragments arranged by size: 5226 702 328 129 PciI A'CATG_T Cuts at: 5284 5284 Size: 6385 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6385 PfoI T'CCnGG_A Cuts at: 2448 2874 2448 Size: 426 5959 PmeI GTTT'AAAC Cuts at: 3689 3689 Size: 6385 PmlI CAC'GTG Cuts at: 3523 3649 3523 Size: 126 6259 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4454 4486 5967 5999 4454 Size: 32 1481 32 4840 Fragments arranged by size: 4840 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2011 2374 2911 2011 Size: 363 537 5485 Fragments arranged by size: 5485 537 363 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6385 PsiI TTA'TAA Cuts at: 3955 3955 Size: 6385 PspOMI G'GGCC_C Cuts at: 2271 2281 2271 Size: 10 6375 PspXI vC'TCGA_Gb Cuts at: 2843 2843 Size: 6385 PstI C_TGCA'G Cuts at: 830 2655 2697 3300 3737 830 Size: 1825 42 603 437 3478 Fragments arranged by size: 3478 1825 603 437 42 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6385 PvuII CAG'CTG Cuts at: 2096 2800 2096 Size: 704 5681 RsrII CG'GwC_CG Cuts at: 2766 2766 Size: 6385 SacI G_AGCT'C Cuts at: 3405 3405 Size: 6385 SacII CC_GC'GG Cuts at: 2662 2765 2838 3006 2662 Size: 103 73 168 6041 Fragments arranged by size: 6041 168 103 73 SalI G'TCGA_C Cuts at: 1936 3727 1936 Size: 1791 4594 SanDI GG'GwC_CC Cuts at: 2374 2911 2374 Size: 537 5848 SbfI CC_TGCA'GG Cuts at: 3737 3737 Size: 6385 ScaI AGT'ACT Cuts at: 4633 6345 4633 Size: 1712 4673 SexAI A'CCwGG_T Cuts at: 2589 2589 Size: 6385 SfcI C'TryA_G Cuts at: 826 1045 2651 2693 3296 3733 4868 5549 5740 Size: 219 1606 42 603 437 1135 681 191 Cuts at: 5740 826 Size: 1471 Fragments arranged by size: 1606 1471 1135 681 603 437 219 191 42 SfiI GGCCn_nnn'nGGCC Cuts at: 3252 3554 3252 Size: 302 6083 SfoI GGC'GCC Cuts at: 3111 3111 Size: 6385 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6385 SgrAI Cr'CCGG_yG Cuts at: 1700 2318 1700 Size: 618 5767 SmaI CCC'GGG Cuts at: 1872 2924 3186 3712 1872 Size: 1052 262 526 4545 Fragments arranged by size: 4545 1052 526 262 SmlI C'TyrA_G Cuts at: 820 1017 1943 2843 4489 5390 5652 820 Size: 197 926 900 1646 901 262 1553 Fragments arranged by size: 1646 1553 926 901 900 262 197 SnaBI TAC'GTA Cuts at: 493 4176 493 Size: 3683 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6385 SphI G_CATG'C Cuts at: 1132 3743 1132 Size: 2611 3774 SrfI GCCC'GGGC Cuts at: 2924 2924 Size: 6385 SspI AAT'ATT Cuts at: 5 52 4309 5 Size: 47 4257 2081 Fragments arranged by size: 4257 2081 47 StuI AGG'CCT Cuts at: 1517 2249 1517 Size: 732 5653 StyI C'CwwG_G Cuts at: 513 1065 3824 513 Size: 552 2759 3074 Fragments arranged by size: 3074 2759 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2872 4412 4429 4582 4767 5212 325 Size: 1255 1292 1540 17 153 185 445 1498 Fragments arranged by size: 1540 1498 1292 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4631 6343 96 Size: 274 80 33 51 1443 2654 1712 138 Fragments arranged by size: 2654 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3064 3096 6345 6377 3064 Size: 32 3249 32 3072 Fragments arranged by size: 3249 3072 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6385 XbaI T'CTAG_A Cuts at: 3721 3721 Size: 6385 XhoI C'TCGA_G Cuts at: 1943 2843 1943 Size: 900 5485 XmaI C'CCGG_G Cuts at: 1870 2922 3184 3710 1870 Size: 1052 262 526 4545 Fragments arranged by size: 4545 1052 526 262 XmnI GAAnn'nnTTC Cuts at: 4514 4514 Size: 6385 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4193 276 Size: 53 83 186 936 2659 2468 Fragments arranged by size: 2659 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AvrII BaeI BfrBI BplI BsiWI BstZ17I EcoRV FalI FseI FspAI NruI NsiI PacI PsrI SapI SwaI XcmI