################################## # NO CUT ENZYMES ( 154 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AfaI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaHI BsaI BseAI BseRI BsiHKAI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DdeI DraI DraIII DrdI DsaI Eam1105I Eco47III Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MaeI MfeI MluI MluNI MroI MscI MseI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfoI SfuI SgrAI SmlI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI Tru9I Tsp509I Tth111I Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AflIII 368 ACATGT A^C[GA][CT]GT AlwNI 232 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG AvaI 27 CCCGGG C^[CT]CG[GA]G BbrPI 217 CACGTG CAC^GTG BpmI 499 CTGGAG CTGGAG(16/14) BsaAI 217 CACGTG [CT]AC^GT[GA] BsaWI 190 ACCGGA [AT]^CCGG[AT] BsgI 334 GTGCAG GTGCAG(16/14) BsiEI 241 CGGCCG CG[GA][CT]^CG BsoBI 27 CCCGGG C^[CT]CG[GA]G BspLU11I 368 ACATGT A^CATGT BstAPI 231 GCAGGTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstF5I 204 CATCC GGATG(2/0) CfoI 13 GCGC GCG^C EagI 241 CGGCCG C^GGCCG EclXI 241 CGGCCG C^GGCCG Eco52I 241 CGGCCG C^GGCCG EcoNI 486 CCTGGTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 389 CCAAGG C^C[AT][AT]GG FokI 204 CATCC GGATG(9/13) HgaI 360 GACGC GACGC(5/10) HhaI 13 GCGC GCG^C HinP1I 13 GCGC G^CGC MaeIII 358 GTGAC ^GT[ACGT]AC PleI 325 GACTC GAGTC(4/5) PmaCI 217 CACGTG CAC^GTG PmlI 217 CACGTG CAC^GTG PpuMI 233 AGGTCCT [GA]G^G[AT]CC[CT] PstI 229 CTGCAG CTGCA^G PvuII 431 CAGCTG CAG^CTG SfaNI 203 GCATC GCATC(5/9) SfcI 229 CTGCAG C^T[GA][CT]AG SmaI 27 CCCGGG CCC^GGG SphI 261 GCATGC GCATG^C StyI 389 CCAAGG C^C[AT][AT]GG TaqI 470 TCGA T^CGA TfiI 467 GAATC G^A[AT]TC Tsp45I 358 GTGAC ^GT[GC]AC TspRI 314 CAGTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 470 TCGA T^CGA XmaCI 27 CCCGGG C^CCGGG XmaI 27 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AccII 12 CGCG CG^CG 446 CGCG AluI 419 AGCT AG^CT 432 AGCT AlwI 456 GATCC GGATC(4/5) 462 GATCC AvaII 234 GGTCC G^G[AT]CC 375 GGACC BanI 154 GGTGCC G^G[CT][GA]CC 274 GGCACC BmyI 178 GTGCCC G[AGT]GC[ACT]^C 220 GTGCCC BsaBI 456 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 462 GATCCGAATC BsmFI 30 GGGAC GGGAC(10/14) 427 GGGAC Bsp1286I 178 GTGCCC G[AGT]GC[ACT]^C 220 GTGCCC BstUI 12 CGCG CG^CG 446 CGCG BstYI 455 AGATCC [GA]^GATC[CT] 461 AGATCC DpnII 456 GATC ^GATC 462 GATC EarI 327 CTCTTC CTCTTC(1/4) 349 GAAGAG FauI 7 CCCGC CCCGC(4/6) 447 GCGGG HinfI 325 GACTC G^A[ACGT]TC 467 GAATC HphI 489 GGTGA GGTGA(8/7) 119 TCACC Ksp632I 327 CTCTTC CTCTTC(1/4) 349 GAAGAG MamI 456 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 462 GATCCGAATC MboI 456 GATC ^GATC 462 GATC MflI 455 AGATCC [GA]^GATC[CT] 461 AGATCC MspA1I 300 CAGCGG C[AC]G^C[GT]G 431 CAGCTG MvnI 12 CGCG CG^CG 446 CGCG NdeII 456 GATC ^GATC 462 GATC NlaIII 262 CATG CATG^ 369 CATG NspI 261 GCATGC [GA]CATG^[CT] 368 ACATGT Sau3AI 456 GATC ^GATC 462 GATC SfiI 345 GGCCGAAGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 474 GGCCAACGAGGCC XcmI 389 CCAAGGCAAACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 476 CCAACGAGGCCCTGG XhoII 455 AGATCC [GA]^GATC[CT] 461 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 33 enzymes) # ######################################### AciI BbvI BcnI BglI BsaJI BsiYI BslI BspMI BssKI BstNI Cac8I Cfr13I DraII EaeI EcoO109I EcoRII Fnu4HI HaeIII HapII HpaII ItaI MaeII MboII MnlI MslI MspI MvaI MwoI NciI NlaIV Sau96I ScrFI TseI