(Circular) (Six-base) MAPSORT of: pFN21AE6186.seq Check: 826 from: 1 to: 5209 pFN21AE6186 5209 bp With 182 enzymes: SgfI * December 8, 2014 13:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2134 2164 2134 Size: 30 5179 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3019 278 Size: 53 83 186 936 1483 2468 Fragments arranged by size: 2468 1483 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2552 1937 Size: 615 4594 Acc65I G'GTAC_C Cuts at: 2530 2530 Size: 5209 AclI AA'CG_TT Cuts at: 1563 3336 3709 1563 Size: 1773 373 3063 Fragments arranged by size: 3063 1773 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3272 4656 4924 1632 Size: 1640 1384 268 1917 Fragments arranged by size: 1917 1640 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5012 AflIII A'CryG_T Cuts at: 1203 2373 4108 5085 1203 Size: 1170 1735 977 1327 Fragments arranged by size: 1735 1327 1170 977 AgeI A'CCGG_T Cuts at: 4040 4040 Size: 5209 AhdI GACnn_n'nnGTC Cuts at: 3938 3938 Size: 5209 AleI CACnn'nnGTG Cuts at: 1166 2061 2144 1166 Size: 895 83 4231 Fragments arranged by size: 4231 895 83 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5209 AlwNI CAG_nnn'CTG Cuts at: 2242 2952 4524 2242 Size: 710 1572 2927 Fragments arranged by size: 2927 1572 710 ApaLI G'TGCA_C Cuts at: 3266 4422 3266 Size: 1156 4053 ApoI r'AATT_y Cuts at: 1493 2518 2744 2896 5005 1493 Size: 1025 226 152 2109 1697 Fragments arranged by size: 2109 1697 1025 226 152 AseI AT'TA_AT Cuts at: 160 1032 3763 160 Size: 872 2731 1606 Fragments arranged by size: 2731 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5209 AvaI C'yCGr_G Cuts at: 1870 1943 2032 2534 1870 Size: 73 89 502 4545 Fragments arranged by size: 4545 502 89 73 BamHI G'GATC_C Cuts at: 2539 2906 2539 Size: 367 4842 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2159 2279 2530 3985 618 Size: 325 217 627 372 120 251 1455 1842 Fragments arranged by size: 1842 1455 627 372 325 251 217 120 BanII G_rGCy'C Cuts at: 1391 2528 1391 Size: 1137 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4238 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3400 3434 1903 Size: 34 50 34 1379 34 3678 Fragments arranged by size: 3678 1379 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3101 4311 3101 Size: 1210 3999 BclI T'GATC_A Cuts at: 1548 1548 Size: 5209 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2090 2357 2453 Size: 107 122 71 631 605 418 267 96 Cuts at: 2453 2486 3820 136 Size: 33 1334 1525 Fragments arranged by size: 1525 1334 631 605 418 267 122 107 96 71 33 BglII A'GATC_T Cuts at: 5204 5204 Size: 5209 BlpI GC'TnA_GC Cuts at: 2626 2626 Size: 5209 Bme1580I G_kGCm'C Cuts at: 1790 2187 2229 3270 4426 1790 Size: 397 42 1041 1156 2573 Fragments arranged by size: 2573 1156 1041 397 42 BmgBI CAC'GTC Cuts at: 1318 2182 1318 Size: 864 4345 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2940 3898 442 Size: 416 53 83 399 24 1523 958 1753 Fragments arranged by size: 1753 1523 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5209 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2525 3869 1239 Size: 1286 1344 2579 Fragments arranged by size: 2579 1344 1286 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3334 4199 4497 3334 Size: 865 298 4046 Fragments arranged by size: 4046 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3872 882 Size: 617 2373 2219 Fragments arranged by size: 2373 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2224 3000 493 Size: 711 1020 776 2702 Fragments arranged by size: 2702 1020 776 711 BsaBI GATnn'nnATC Cuts at: 2465 2471 5203 2465 Size: 6 2732 2471 Fragments arranged by size: 2732 2471 6 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3016 3398 4033 275 Size: 53 83 186 936 1483 382 635 1451 Fragments arranged by size: 1483 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2195 3642 4040 4314 4461 4872 2195 Size: 1447 398 274 147 411 2532 Fragments arranged by size: 2532 1447 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4984 Fragments arranged by size: 4984 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5209 BseYI C'CCAG_C Cuts at: 4412 4908 4412 Size: 496 4713 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2360 1163 Size: 573 624 4012 Fragments arranged by size: 4012 624 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2249 2659 3420 4448 1540 Size: 275 186 248 410 761 1028 2301 Fragments arranged by size: 2301 1028 761 410 275 248 186 BsiHKAI G_wGCw'C Cuts at: 3270 3355 4426 3270 Size: 85 1071 4053 Fragments arranged by size: 4053 1071 85 BsmI GAATG_Cn' Cuts at: 2720 2813 2720 Size: 93 5116 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2187 2229 2528 3270 3355 4426 1391 Size: 399 397 42 299 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 397 299 85 42 BspEI T'CCGG_A Cuts at: 4872 4872 Size: 5209 BspHI T'CATG_A Cuts at: 3098 4828 3098 Size: 1730 3479 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2134 2164 2227 2564 844 Size: 1290 30 63 337 3489 Fragments arranged by size: 3489 1290 337 63 30 BsrBI CCG'CTC Cuts at: 1394 1823 3096 1394 Size: 429 1273 3507 Fragments arranged by size: 3507 1273 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3704 3878 65 Size: 1183 2456 174 1396 Fragments arranged by size: 2456 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3853 4040 1700 Size: 110 2043 187 2869 Fragments arranged by size: 2869 2043 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5209 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5209 BssSI C'ACGA_G Cuts at: 3269 4281 3269 Size: 1012 4197 BstAPI GCAn_nnn'nTGC Cuts at: 794 2242 794 Size: 1448 3761 BstBI TT'CG_AA Cuts at: 4013 4013 Size: 5209 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5209 BstYI r'GATC_y Cuts at: 1972 2460 2466 2539 2906 3291 3308 4760 4846 Size: 488 6 73 367 385 17 1452 86 Cuts at: 4846 4858 5204 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 488 385 367 346 86 73 17 12 6 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4657 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2299 487 Size: 683 56 1073 3397 Fragments arranged by size: 3397 1073 683 56 BtsI GCAGTG_nn' Cuts at: 2325 2725 3519 3539 2325 Size: 400 794 20 3995 Fragments arranged by size: 3995 794 400 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5209 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5174 DraI TTT'AAA Cuts at: 2513 2513 Size: 5209 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4216 809 Size: 507 2900 1802 Fragments arranged by size: 2900 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2041 2246 2349 Size: 54 1425 50 275 18 211 205 103 Cuts at: 2349 2656 3545 5174 8 Size: 307 889 1629 43 Fragments arranged by size: 1629 1425 889 307 275 211 205 103 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2246 2656 1537 Size: 275 434 410 4090 Fragments arranged by size: 4090 434 410 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2338 2349 3139 1353 Size: 985 11 790 3423 Fragments arranged by size: 3423 985 790 11 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2015 2042 2435 3796 4170 4316 Size: 1064 138 426 27 393 1361 374 146 Cuts at: 4316 387 Size: 1280 Fragments arranged by size: 1361 1280 1064 426 393 374 146 138 27 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2525 3272 3869 4656 4924 1239 Size: 393 893 747 597 787 268 1524 Fragments arranged by size: 1524 893 787 747 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2495 2495 Size: 5209 EcoO109I rG'GnC_Cy Cuts at: 2239 2488 2500 2239 Size: 249 12 4948 Fragments arranged by size: 4948 249 12 EcoRI G'AATT_C Cuts at: 2518 2896 2518 Size: 378 4831 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2419 2419 Size: 5209 FspI TGC'GCA Cuts at: 3715 3715 Size: 5209 HaeII r_GCGC'y Cuts at: 1678 4356 1678 Size: 2678 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3850 3882 3924 3956 1373 Size: 32 135 32 2278 32 42 32 2626 Fragments arranged by size: 2626 2278 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2553 2799 669 Size: 988 281 615 246 3079 Fragments arranged by size: 3079 988 615 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5209 HpaI GTT'AAC Cuts at: 2799 2799 Size: 5209 KpnI G_GTAC'C Cuts at: 2534 2534 Size: 5209 MfeI C'AATT_G Cuts at: 2808 2808 Size: 5209 MluI A'CGCG_T Cuts at: 5085 5085 Size: 5209 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2358 4323 4507 1305 Size: 1053 1965 184 2007 Fragments arranged by size: 2007 1965 1053 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3387 Fragments arranged by size: 3387 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2061 2144 2318 3167 3526 3685 518 Size: 648 895 83 174 849 359 159 2042 Fragments arranged by size: 2042 895 849 648 359 174 159 83 MspA1I CmG'CkG Cuts at: 1606 2307 2438 2625 3302 4450 4695 1606 Size: 701 131 187 677 1148 245 2120 Fragments arranged by size: 2120 1148 701 677 245 187 131 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4657 NdeI CA'TA_TG Cuts at: 387 387 Size: 5209 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5209 NotI GC'GGCC_GC Cuts at: 2656 2656 Size: 5209 NspI r_CATG'y Cuts at: 1132 1229 2270 2377 2567 4112 1132 Size: 97 1041 107 190 1545 2229 Fragments arranged by size: 2229 1545 1041 190 107 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5209 PciI A'CATG_T Cuts at: 2373 4108 2373 Size: 1735 3474 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5209 PmeI GTTT'AAAC Cuts at: 2513 2513 Size: 5209 PmlI CAC'GTG Cuts at: 2224 2224 Size: 5209 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2293 2315 2325 2347 3278 3310 4791 4823 2293 Size: 22 10 22 931 32 1481 32 2679 Fragments arranged by size: 2679 1481 931 32 32 22 22 10 PpuMI rG'GwC_Cy Cuts at: 2239 2239 Size: 5209 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5209 PsiI TTA'TAA Cuts at: 2779 2779 Size: 5209 PstI C_TGCA'G Cuts at: 830 2238 2561 830 Size: 1408 323 3478 Fragments arranged by size: 3478 1408 323 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5209 PvuII CAG'CTG Cuts at: 2438 2438 Size: 5209 SalI G'TCGA_C Cuts at: 1936 2551 1936 Size: 615 4594 SbfI CC_TGCA'GG Cuts at: 2561 2561 Size: 5209 ScaI AGT'ACT Cuts at: 3457 5169 3457 Size: 1712 3497 SfcI C'TryA_G Cuts at: 826 1045 2234 2557 3692 4373 4564 826 Size: 219 1189 323 1135 681 191 1471 Fragments arranged by size: 1471 1189 1135 681 323 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2357 2486 2357 Size: 129 5080 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5209 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5209 SmaI CCC'GGG Cuts at: 1872 2034 2536 1872 Size: 162 502 4545 Fragments arranged by size: 4545 502 162 SmlI C'TyrA_G Cuts at: 820 1017 1943 3313 4214 4476 820 Size: 197 926 1370 901 262 1553 Fragments arranged by size: 1553 1370 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3000 493 Size: 2507 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5209 SphI G_CATG'C Cuts at: 1132 2270 2567 1132 Size: 1138 297 3774 Fragments arranged by size: 3774 1138 297 SspI AAT'ATT Cuts at: 5 52 3133 5 Size: 47 3081 2081 Fragments arranged by size: 3081 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5209 StyI C'CwwG_G Cuts at: 513 1065 2394 2648 513 Size: 552 1329 254 3074 Fragments arranged by size: 3074 1329 552 254 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3236 3253 3406 3591 4036 325 Size: 1255 1656 17 153 185 445 1498 Fragments arranged by size: 1656 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3455 5167 96 Size: 274 80 33 51 1443 1478 1712 138 Fragments arranged by size: 1712 1478 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5169 5201 5169 Size: 32 5177 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5209 XbaI T'CTAG_A Cuts at: 2545 2545 Size: 5209 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2401 2488 2401 Size: 87 5122 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5209 XmaI C'CCGG_G Cuts at: 1870 2032 2534 1870 Size: 162 502 4545 Fragments arranged by size: 4545 502 162 XmnI GAAnn'nnTTC Cuts at: 3338 3338 Size: 5209 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3017 276 Size: 53 83 186 936 1483 2468 Fragments arranged by size: 2468 1483 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfoI SrfI SwaI