(Circular) (Six-base) MAPSORT of: pFN21AE1863.seq Check: 2428 from: 1 to: 5851 pFN21AE1863 5851 bp With 182 enzymes: SgfI * August 16, 2010 14:53 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2074 2150 2074 Size: 76 5775 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3661 278 Size: 53 83 186 936 2125 2468 Fragments arranged by size: 2468 2125 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3194 1937 Size: 1257 4594 Acc65I G'GTAC_C Cuts at: 3172 3172 Size: 5851 AclI AA'CG_TT Cuts at: 1563 3978 4351 1563 Size: 2415 373 3063 Fragments arranged by size: 3063 2415 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3914 5298 5566 1632 Size: 2282 1384 268 1917 Fragments arranged by size: 2282 1917 1384 268 AfeI AGC'GCT Cuts at: 2567 2567 Size: 5851 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5654 AflIII A'CryG_T Cuts at: 1203 4750 5727 1203 Size: 3547 977 1327 Fragments arranged by size: 3547 1327 977 AgeI A'CCGG_T Cuts at: 4682 4682 Size: 5851 AhdI GACnn_n'nnGTC Cuts at: 4580 4580 Size: 5851 AleI CACnn'nnGTG Cuts at: 1166 2426 1166 Size: 1260 4591 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3002 1184 Size: 1818 4033 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2534 2566 2534 Size: 32 5819 AlwNI CAG_nnn'CTG Cuts at: 2476 3594 5166 2476 Size: 1118 1572 3161 Fragments arranged by size: 3161 1572 1118 ApaI G_GGCC'C Cuts at: 2501 2904 2501 Size: 403 5448 ApaLI G'TGCA_C Cuts at: 3908 5064 3908 Size: 1156 4695 ApoI r'AATT_y Cuts at: 1493 3160 3386 3538 5647 1493 Size: 1667 226 152 2109 1697 Fragments arranged by size: 2109 1697 1667 226 152 AseI AT'TA_AT Cuts at: 160 1032 4405 160 Size: 872 3373 1606 Fragments arranged by size: 3373 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5851 AvaI C'yCGr_G Cuts at: 1870 1943 3176 1870 Size: 73 1233 4545 Fragments arranged by size: 4545 1233 73 BamHI G'GATC_C Cuts at: 3181 3548 3181 Size: 367 5484 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2123 2380 3042 3172 4627 Size: 325 217 627 336 257 662 130 1455 Cuts at: 4627 618 Size: 1842 Fragments arranged by size: 1842 1455 662 627 336 325 257 217 130 BanII G_rGCy'C Cuts at: 1391 2049 2094 2294 2501 2529 2649 2820 2837 Size: 658 45 200 207 28 120 171 17 Cuts at: 2837 2858 2904 3170 1391 Size: 21 46 266 4072 Fragments arranged by size: 4072 658 266 207 200 171 120 46 45 28 21 17 BbeI G_GCGC'C Cuts at: 2127 2127 Size: 5851 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4880 BbvCI CC'TCA_GC Cuts at: 3051 3051 Size: 5851 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2614 2648 4042 4076 1903 Size: 34 677 34 1394 34 3678 Fragments arranged by size: 3678 1394 677 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3743 4953 3743 Size: 1210 4641 BclI T'GATC_A Cuts at: 1548 1548 Size: 5851 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2408 4462 136 Size: 107 122 71 631 605 736 2054 1525 Fragments arranged by size: 2054 1525 736 631 605 122 107 71 BglII A'GATC_T Cuts at: 2886 5846 2886 Size: 2960 2891 BlpI GC'TnA_GC Cuts at: 2648 3268 2648 Size: 620 5231 Bme1580I G_kGCm'C Cuts at: 1790 2501 2904 3912 5068 1790 Size: 711 403 1008 1156 2573 Fragments arranged by size: 2573 1156 1008 711 403 BmgBI CAC'GTC Cuts at: 1318 2545 1318 Size: 1227 4624 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3582 4540 442 Size: 416 53 83 399 24 2165 958 1753 Fragments arranged by size: 2165 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5851 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2051 2498 4511 1239 Size: 812 447 2013 2579 Fragments arranged by size: 2579 2013 812 447 Bpu10I CC'TnA_GC Cuts at: 3051 3051 Size: 5851 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3976 4841 5139 3976 Size: 865 298 4688 Fragments arranged by size: 4688 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4514 882 Size: 617 3015 2219 Fragments arranged by size: 3015 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3642 493 Size: 711 2438 2702 Fragments arranged by size: 2702 2438 711 BsaBI GATnn'nnATC Cuts at: 5845 5845 Size: 5851 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2124 3658 4040 4675 Size: 53 83 186 936 591 1534 382 635 Cuts at: 4675 275 Size: 1451 Fragments arranged by size: 1534 1451 936 635 591 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4284 4682 4956 5103 5514 4284 Size: 398 274 147 411 4621 Fragments arranged by size: 4621 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2007 2037 2429 2459 2696 Size: 30 165 30 604 30 392 30 237 Cuts at: 2696 2720 2726 2744 2750 2768 2774 2792 2798 Size: 24 6 18 6 18 6 18 6 Cuts at: 2798 2822 2985 3015 1178 Size: 24 163 30 4014 Fragments arranged by size: 4014 604 392 237 165 163 30 30 30 30 30 24 24 18 18 18 6 6 6 6 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2039 2042 2528 2531 2534 2537 1186 Size: 853 3 486 3 3 3 4500 Fragments arranged by size: 4500 853 486 3 3 3 3 BseYI C'CCAG_C Cuts at: 2368 2826 2897 2989 3110 5054 5550 2368 Size: 458 71 92 121 1944 496 2669 Fragments arranged by size: 2669 1944 496 458 121 92 71 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2198 2234 2345 2528 2660 1163 Size: 573 462 36 111 183 132 4354 Fragments arranged by size: 4354 573 462 183 132 111 36 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2271 3301 4062 5090 1540 Size: 275 186 270 1030 761 1028 2301 Fragments arranged by size: 2301 1030 1028 761 275 270 186 BsiHKAI G_wGCw'C Cuts at: 2649 3912 3997 5068 2649 Size: 1263 85 1071 3432 Fragments arranged by size: 3432 1263 1071 85 BsmI GAATG_Cn' Cuts at: 3362 3455 3362 Size: 93 5758 BsmBI CGTCTCn'nnnn_ Cuts at: 2368 2368 Size: 5851 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2049 2094 2294 2501 2529 2649 2820 Size: 399 259 45 200 207 28 120 171 Cuts at: 2820 2837 2858 2904 3170 3912 3997 5068 1391 Size: 17 21 46 266 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 266 259 207 200 171 120 85 46 45 28 21 17 BspEI T'CCGG_A Cuts at: 5514 5514 Size: 5851 BspHI T'CATG_A Cuts at: 3740 5470 3740 Size: 1730 4121 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2074 2150 2950 3206 844 Size: 1230 76 800 256 3489 Fragments arranged by size: 3489 1230 800 256 76 BsrBI CCG'CTC Cuts at: 1394 1823 3738 1394 Size: 429 1915 3507 Fragments arranged by size: 3507 1915 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4346 4520 65 Size: 1183 3098 174 1396 Fragments arranged by size: 3098 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2101 3039 3125 4495 4682 1700 Size: 110 291 938 86 1370 187 2869 Fragments arranged by size: 2869 1370 938 291 187 110 86 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5851 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5851 BssSI C'ACGA_G Cuts at: 3911 4923 3911 Size: 1012 4839 BstAPI GCAn_nnn'nTGC Cuts at: 794 2323 794 Size: 1529 4322 BstBI TT'CG_AA Cuts at: 4655 4655 Size: 5851 BstEII G'GTnAC_C Cuts at: 1184 2387 2948 1184 Size: 1203 561 4087 Fragments arranged by size: 4087 1203 561 BstYI r'GATC_y Cuts at: 1972 2886 3181 3548 3933 3950 5402 5488 5500 Size: 914 295 367 385 17 1452 86 12 Cuts at: 5500 5846 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 914 385 367 346 295 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2112 2164 2427 2961 513 Size: 552 939 108 52 263 534 3403 Fragments arranged by size: 3403 939 552 534 263 108 52 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5112 Fragments arranged by size: 5112 683 56 BtsI GCAGTG_nn' Cuts at: 2186 2986 3367 4161 4181 2186 Size: 800 381 794 20 3856 Fragments arranged by size: 3856 800 794 381 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5851 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5816 DraI TTT'AAA Cuts at: 3155 3155 Size: 5851 DraIII CAC_nnn'GTG Cuts at: 2323 2323 Size: 5851 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4858 809 Size: 507 3542 1802 Fragments arranged by size: 3542 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2056 2109 2268 Size: 54 1425 50 275 18 226 53 159 Cuts at: 2268 3127 3298 4187 5816 8 Size: 859 171 889 1629 43 Fragments arranged by size: 1629 1425 889 859 275 226 171 159 54 53 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2268 3298 1537 Size: 275 456 1030 4090 Fragments arranged by size: 4090 1030 456 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3781 1353 Size: 2428 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4438 4812 4958 387 Size: 1064 138 2849 374 146 1280 Fragments arranged by size: 2849 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2647 2647 Size: 5851 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2051 2498 3914 4511 5298 5566 1239 Size: 393 419 447 1416 597 787 268 1524 Fragments arranged by size: 1524 1416 787 597 447 419 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 3050 3050 Size: 5851 EcoO109I rG'GnC_Cy Cuts at: 2169 2188 2274 2589 2169 Size: 19 86 315 5431 Fragments arranged by size: 5431 315 86 19 EcoRI G'AATT_C Cuts at: 3160 3538 3160 Size: 378 5473 FspI TGC'GCA Cuts at: 2249 4357 2249 Size: 2108 3743 HaeII r_GCGC'y Cuts at: 1678 2127 2569 4998 1678 Size: 449 442 2429 2531 Fragments arranged by size: 2531 2429 449 442 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2007 2039 2696 2720 2728 Size: 32 135 32 435 32 657 24 8 Cuts at: 2728 2744 2752 2768 2776 2792 2800 2824 2904 Size: 16 8 16 8 16 8 24 80 Cuts at: 2904 2936 2985 3017 4492 4524 4566 4598 1373 Size: 32 49 32 1475 32 42 32 2626 Fragments arranged by size: 2626 1475 657 435 135 80 49 42 32 32 32 32 32 32 32 24 24 16 16 16 8 8 8 8 HincII GTy'rAC Cuts at: 669 1657 1938 3195 3441 669 Size: 988 281 1257 246 3079 Fragments arranged by size: 3079 1257 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5851 HpaI GTT'AAC Cuts at: 3441 3441 Size: 5851 KasI G'GCGC_C Cuts at: 2123 2123 Size: 5851 KpnI G_GTAC'C Cuts at: 3176 3176 Size: 5851 MfeI C'AATT_G Cuts at: 3450 3450 Size: 5851 MluI A'CGCG_T Cuts at: 5727 5727 Size: 5851 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2199 4965 5149 1305 Size: 894 2766 184 2007 Fragments arranged by size: 2766 2007 894 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4029 Fragments arranged by size: 4029 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2426 3809 4168 4327 518 Size: 648 1260 1383 359 159 2042 Fragments arranged by size: 2042 1383 1260 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2166 2356 2956 2963 3267 3944 5092 5337 Size: 560 190 600 7 304 677 1148 245 Cuts at: 5337 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 600 560 304 245 190 7 NaeI GCC'GGC Cuts at: 3041 3041 Size: 5851 NarI GG'CG_CC Cuts at: 2124 2124 Size: 5851 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4360 Fragments arranged by size: 4360 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5851 NgoMIV G'CCGG_C Cuts at: 3039 3039 Size: 5851 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5851 NotI GC'GGCC_GC Cuts at: 3298 3298 Size: 5851 NspI r_CATG'y Cuts at: 1132 1229 3209 4754 1132 Size: 97 1980 1545 2229 Fragments arranged by size: 2229 1980 1545 97 PasI CC'CwG_GG Cuts at: 1348 2494 2530 2584 1348 Size: 1146 36 54 4615 Fragments arranged by size: 4615 1146 54 36 PciI A'CATG_T Cuts at: 4750 4750 Size: 5851 PflMI CCAn_nnn'nTGG Cuts at: 1108 2476 1108 Size: 1368 4483 PfoI T'CCnGG_A Cuts at: 2346 2346 Size: 5851 PmeI GTTT'AAAC Cuts at: 3155 3155 Size: 5851 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3920 3952 5433 5465 3920 Size: 32 1481 32 4306 Fragments arranged by size: 4306 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2274 2589 2274 Size: 315 5536 PshAI GACnn'nnGTC Cuts at: 1613 2594 1613 Size: 981 4870 PsiI TTA'TAA Cuts at: 3421 3421 Size: 5851 PspOMI G'GGCC_C Cuts at: 2497 2900 2497 Size: 403 5448 PstI C_TGCA'G Cuts at: 830 2085 3203 830 Size: 1255 1118 3478 Fragments arranged by size: 3478 1255 1118 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5851 PvuII CAG'CTG Cuts at: 2356 2956 2356 Size: 600 5251 RsrII CG'GwC_CG Cuts at: 2161 2161 Size: 5851 SacI G_AGCT'C Cuts at: 2649 2649 Size: 5851 SacII CC_GC'GG Cuts at: 2167 2964 2167 Size: 797 5054 SalI G'TCGA_C Cuts at: 1936 3193 1936 Size: 1257 4594 SanDI GG'GwC_CC Cuts at: 2274 2589 2274 Size: 315 5536 SbfI CC_TGCA'GG Cuts at: 2085 3203 2085 Size: 1118 4733 ScaI AGT'ACT Cuts at: 4099 5811 4099 Size: 1712 4139 SfcI C'TryA_G Cuts at: 826 1045 2081 3199 4334 5015 5206 826 Size: 219 1036 1118 1135 681 191 1471 Fragments arranged by size: 1471 1135 1118 1036 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2408 2408 Size: 5851 SfoI GGC'GCC Cuts at: 2125 2125 Size: 5851 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5851 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5851 SmaI CCC'GGG Cuts at: 1872 3178 1872 Size: 1306 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3955 4856 5118 820 Size: 197 926 2012 901 262 1553 Fragments arranged by size: 2012 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3642 493 Size: 3149 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5851 SphI G_CATG'C Cuts at: 1132 3209 1132 Size: 2077 3774 SspI AAT'ATT Cuts at: 5 52 3775 5 Size: 47 3723 2081 Fragments arranged by size: 3723 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5851 StyI C'CwwG_G Cuts at: 513 1065 2004 2118 3290 513 Size: 552 939 114 1172 3074 Fragments arranged by size: 3074 1172 939 552 114 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3077 3081 3878 3895 4048 4233 4678 Size: 1255 1497 4 797 17 153 185 445 Cuts at: 4678 325 Size: 1498 Fragments arranged by size: 1498 1497 1255 797 445 185 153 17 4 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4097 5809 96 Size: 274 80 33 51 1443 2120 1712 138 Fragments arranged by size: 2120 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2965 2997 5811 5843 2965 Size: 32 2814 32 2973 Fragments arranged by size: 2973 2814 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5851 XbaI T'CTAG_A Cuts at: 3187 3187 Size: 5851 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5851 XmaI C'CCGG_G Cuts at: 1870 3176 1870 Size: 1306 4545 XmnI GAAnn'nnTTC Cuts at: 3980 3980 Size: 5851 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3659 276 Size: 53 83 186 936 2125 2468 Fragments arranged by size: 2468 2125 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspXI PsrI SapI SexAI SrfI SwaI XcmI