(Linear) (Six-base) MAPSORT of: KIEE1863.seq Check: 868 from: 1 to: 1146 KIEE1863.orf With 182 enzymes: SgfI * July 15, 2010 18:49 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 69 145 1146 Size: 69 76 1001 Fragments arranged by size: 1001 76 69 AfeI AGC'GCT Cuts at: 0 562 1146 Size: 562 584 AleI CACnn'nnGTG Cuts at: 0 421 1146 Size: 421 725 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 997 1146 Size: 997 149 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 529 561 1146 Size: 529 32 585 Fragments arranged by size: 585 529 32 AlwNI CAG_nnn'CTG Cuts at: 0 471 1146 Size: 471 675 ApaI G_GGCC'C Cuts at: 0 496 899 1146 Size: 496 403 247 Fragments arranged by size: 496 403 247 BanI G'GyrC_C Cuts at: 0 118 375 1037 1146 Size: 118 257 662 109 Fragments arranged by size: 662 257 118 109 BanII G_rGCy'C Cuts at: 0 44 89 289 496 524 644 815 832 Size: 44 45 200 207 28 120 171 17 Cuts at: 832 853 899 1146 Size: 21 46 247 Fragments arranged by size: 247 207 200 171 120 46 45 44 28 21 17 BbeI G_GCGC'C Cuts at: 0 122 1146 Size: 122 1024 BbvCI CC'TCA_GC Cuts at: 0 1046 1146 Size: 1046 100 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 609 643 1146 Size: 609 34 503 Fragments arranged by size: 609 503 34 BglI GCCn_nnn'nGGC Cuts at: 0 403 1146 Size: 403 743 BglII A'GATC_T Cuts at: 0 881 1146 Size: 881 265 BlpI GC'TnA_GC Cuts at: 0 643 1146 Size: 643 503 Bme1580I G_kGCm'C Cuts at: 0 496 899 1146 Size: 496 403 247 Fragments arranged by size: 496 403 247 BmgBI CAC'GTC Cuts at: 0 540 1146 Size: 540 606 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 46 493 1146 Size: 46 447 653 Fragments arranged by size: 653 447 46 Bpu10I CC'TnA_GC Cuts at: 0 1046 1146 Size: 1046 100 BsaHI Gr'CG_yC Cuts at: 0 119 1146 Size: 119 1027 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 2 32 424 454 691 715 721 739 Size: 2 30 392 30 237 24 6 18 Cuts at: 739 745 763 769 787 793 817 980 1010 Size: 6 18 6 18 6 24 163 30 Cuts at: 1010 1146 Size: 136 Fragments arranged by size: 392 237 163 136 30 30 30 24 24 18 18 18 6 6 6 6 2 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 34 37 523 526 529 532 1146 Size: 34 3 486 3 3 3 614 Fragments arranged by size: 614 486 34 3 3 3 3 BseYI C'CCAG_C Cuts at: 0 363 821 892 984 1105 1146 Size: 363 458 71 92 121 41 Fragments arranged by size: 458 363 121 92 71 41 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 193 229 340 523 655 1146 Size: 193 36 111 183 132 491 Fragments arranged by size: 491 193 183 132 111 36 BsiEI CG_ry'CG Cuts at: 0 266 1146 Size: 266 880 BsiHKAI G_wGCw'C Cuts at: 0 644 1146 Size: 644 502 BsmBI CGTCTCn'nnnn_ Cuts at: 0 363 1146 Size: 363 783 Bsp1286I G_dGCh'C Cuts at: 0 44 89 289 496 524 644 815 832 Size: 44 45 200 207 28 120 171 17 Cuts at: 832 853 899 1146 Size: 21 46 247 Fragments arranged by size: 247 207 200 171 120 46 45 44 28 21 17 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 69 145 945 1146 Size: 69 76 800 201 Fragments arranged by size: 800 201 76 69 BsrFI r'CCGG_y Cuts at: 0 96 1034 1120 1146 Size: 96 938 86 26 Fragments arranged by size: 938 96 86 26 BstAPI GCAn_nnn'nTGC Cuts at: 0 318 1146 Size: 318 828 BstEII G'GTnAC_C Cuts at: 0 382 943 1146 Size: 382 561 203 Fragments arranged by size: 561 382 203 BstYI r'GATC_y Cuts at: 0 881 1146 Size: 881 265 BtgI C'CryG_G Cuts at: 0 107 159 422 956 1146 Size: 107 52 263 534 190 Fragments arranged by size: 534 263 190 107 52 BtsI GCAGTG_nn' Cuts at: 0 181 981 1146 Size: 181 800 165 Fragments arranged by size: 800 181 165 DraIII CAC_nnn'GTG Cuts at: 0 318 1146 Size: 318 828 EaeI y'GGCC_r Cuts at: 0 51 104 263 1122 1146 Size: 51 53 159 859 24 Fragments arranged by size: 859 159 53 51 24 EagI C'GGCC_G Cuts at: 0 263 1146 Size: 263 883 EcoICRI GAG'CTC Cuts at: 0 642 1146 Size: 642 504 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 46 493 1146 Size: 46 447 653 Fragments arranged by size: 653 447 46 EcoNI CCTnn'n_nnAGG Cuts at: 0 1045 1146 Size: 1045 101 EcoO109I rG'GnC_Cy Cuts at: 0 164 183 269 584 1146 Size: 164 19 86 315 562 Fragments arranged by size: 562 315 164 86 19 FspI TGC'GCA Cuts at: 0 244 1146 Size: 244 902 HaeII r_GCGC'y Cuts at: 0 122 564 1146 Size: 122 442 582 Fragments arranged by size: 582 442 122 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 2 34 691 715 723 739 747 763 Size: 2 32 657 24 8 16 8 16 Cuts at: 763 771 787 795 819 899 931 980 1012 Size: 8 16 8 24 80 32 49 32 Cuts at: 1012 1146 Size: 134 Fragments arranged by size: 657 134 80 49 32 32 32 24 24 16 16 16 8 8 8 8 2 KasI G'GCGC_C Cuts at: 0 118 1146 Size: 118 1028 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 194 1146 Size: 194 952 MslI CAynn'nnrTG Cuts at: 0 421 1146 Size: 421 725 MspA1I CmG'CkG Cuts at: 0 161 351 951 958 1146 Size: 161 190 600 7 188 Fragments arranged by size: 600 190 188 161 7 NaeI GCC'GGC Cuts at: 0 1036 1146 Size: 1036 110 NarI GG'CG_CC Cuts at: 0 119 1146 Size: 119 1027 NgoMIV G'CCGG_C Cuts at: 0 1034 1146 Size: 1034 112 PasI CC'CwG_GG Cuts at: 0 489 525 579 1146 Size: 489 36 54 567 Fragments arranged by size: 567 489 54 36 PflMI CCAn_nnn'nTGG Cuts at: 0 471 1146 Size: 471 675 PfoI T'CCnGG_A Cuts at: 0 341 1146 Size: 341 805 PpuMI rG'GwC_Cy Cuts at: 0 269 584 1146 Size: 269 315 562 Fragments arranged by size: 562 315 269 PshAI GACnn'nnGTC Cuts at: 0 589 1146 Size: 589 557 PspOMI G'GGCC_C Cuts at: 0 492 895 1146 Size: 492 403 251 Fragments arranged by size: 492 403 251 PstI C_TGCA'G Cuts at: 0 80 1146 Size: 80 1066 PvuII CAG'CTG Cuts at: 0 351 951 1146 Size: 351 600 195 Fragments arranged by size: 600 351 195 RsrII CG'GwC_CG Cuts at: 0 156 1146 Size: 156 990 SacI G_AGCT'C Cuts at: 0 644 1146 Size: 644 502 SacII CC_GC'GG Cuts at: 0 162 959 1146 Size: 162 797 187 Fragments arranged by size: 797 187 162 SanDI GG'GwC_CC Cuts at: 0 269 584 1146 Size: 269 315 562 Fragments arranged by size: 562 315 269 SbfI CC_TGCA'GG Cuts at: 0 80 1146 Size: 80 1066 SfcI C'TryA_G Cuts at: 0 76 1146 Size: 76 1070 SfiI GGCCn_nnn'nGGCC Cuts at: 0 403 1146 Size: 403 743 SfoI GGC'GCC Cuts at: 0 120 1146 Size: 120 1026 StyI C'CwwG_G Cuts at: 0 113 1146 Size: 113 1033 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1072 1076 1146 Size: 1072 4 70 Fragments arranged by size: 1072 70 4 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 960 992 1146 Size: 960 32 154 Fragments arranged by size: 960 154 32 Enzymes that do cut: AarI AfeI AleI AlfI AloI AlwNI ApaI BanI BanII BbeI BbvCI BcgI BglI BglII BlpI Bme1580I BmgBI BpmI Bpu10I BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrFI BstAPI BstEII BstYI BtgI BtsI DraIII EaeI EagI EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I KasI MmeI MslI MspA1I NaeI NarI NgoMIV PasI PflMI PfoI PpuMI PshAI PspOMI PstI PvuII RsrII SacI SacII SanDI SbfI SfcI SfiI SfoI StyI TaqII TstI Enzymes that do not cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbsI BciVI BclI BfrBI BmrI BmtI BplI BpuEI BsaI BsaAI BsaBI BsaWI BsiWI BsmI BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI BstBI BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI EarI EciI EcoRI EcoRV FalI FseI FspAI HincII HindIII HpaI KpnI MfeI MluI MscI NcoI NdeI NheI NotI NruI NsiI NspI PacI PciI PmeI PmlI PpiI PsiI PspXI PsrI PvuI SalI SapI ScaI SexAI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI