################################## # NO CUT ENZYMES ( 147 enzymes) # ################################## Acc65I AccI AccII AclI AfaI AflII AflIII AgeI AhdI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AvaI AvrII BanII BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaI BsgI BsiEI BsiWI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspHI BspLU11I BspMI BsrBI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I CelII ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI HincII HindII HindIII HinfI HpaI KpnI KspI MaeI MamI MluI MvnI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI TspRI TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AatII 526 GACGTC GACGT^C AccIII 50 TCCGGA T^CCGGA AcsI 11 AAATTC [GA]^AATT[CT] Alw44I 265 GTGCAC G^TGCAC AocI 549 CCTGAGG CC^T[ACGT]AGG ApaLI 265 GTGCAC G^TGCAC ApoI 11 AAATTC [GA]^AATT[CT] AspHI 265 GTGCAC G[AT]GC[AT]^C AspI 526 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 214 GGACC G^G[AT]CC AviII 150 TGCGCA TGC^GCA BamHI 252 GGATCC G^GATCC BbeI 112 GGCGCC GGCGC^C BcnI 40 CCCGG CC^[GC]GG BglI 275 GCCACGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaWI 50 TCCGGA [AT]^CCGG[AT] BseAI 50 TCCGGA T^CCGGA BseRI 395 CTCCTC GAGGAG(10/8) BsiHKAI 265 GTGCAC G[AT]GC[AT]^C BspEI 50 TCCGGA T^CCGGA BsrDI 350 CATTGC GCAATG(2/0) BsrI 116 CCAGT ACTGG(1/-1) BssSI 561 CACGAG CACGAG(-5/-1) BstF5I 401 CATCC GGATG(2/0) BstYI 252 GGATCC [GA]^GATC[CT] Bsu36I 549 CCTGAGG CC^T[ACGT]AGG DraII 213 AGGACCT [GA]G^G[ACGT]CC[CT] Eco81I 549 CCTGAGG CC^T[ACGT]AGG EcoNI 549 CCTGAGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 213 AGGACCT [GA]G^G[ACGT]CC[CT] FauI 101 GCGGG CCCGC(4/6) FokI 401 CATCC GGATG(9/13) FseI 68 GGCCGGCC GGCCGG^CC FspI 150 TGCGCA TGC^GCA HaeII 112 GGCGCC [GA]GCGC^[CT] HgaI 133 GCGTC GACGC(5/10) HphI 533 TCACC GGTGA(8/7) KasI 112 GGCGCC G^GCGCC MaeII 527 ACGT A^CGT MaeIII 532 GTCAC ^GT[ACGT]AC MfeI 17 CAATTG C^AATTG MflI 252 GGATCC [GA]^GATC[CT] MroI 50 TCCGGA T^CCGGA MslI 350 CATTGCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 96 CAGCGG C[AC]G^C[GT]G MunI 17 CAATTG C^AATTG NaeI 69 GCCGGC GCC^GGC NarI 112 GGCGCC GG^CGCC NciI 40 CCCGG CC^[GC]GG NgoMIV 69 GCCGGC G^CCGGC PflFI 526 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 213 AGGACCT [GA]G^G[AT]CC[CT] SfoI 112 GGCGCC GGC^GCC Tsp45I 532 GTCAC ^GT[GC]AC Tth111I 526 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoII 252 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AcyI 112 GGCGCC G[GA]^CG[CT]C 526 GACGTC BalI 23 TGGCCA TGG^CCA 273 TGGCCA BanI 112 GGCGCC G^G[CT][GA]CC 192 GGTGCC BmyI 193 GTGCCC G[AGT]GC[ACT]^C 265 GTGCAC BsaHI 112 GGCGCC G[GA]^CG[CT]C 526 GACGTC BsaJI 276 CCACGG C^C[ACGT][ACGT]GG 355 CCGTGG BsiYI 503 CCTACATTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 549 CCTGAGGAAGG BslI 503 CCTACATTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 549 CCTGAGGAAGG Bsp1286I 193 GTGCCC G[AGT]GC[ACT]^C 265 GTGCAC BsrFI 69 GCCGGC [GA]^CCGG[CT] 535 ACCGGC BstNI 311 CCTGG CC^[AT]GG 543 CCTGG Cfr10I 69 GCCGGC [GA]^CCGG[CT] 535 ACCGGC Cfr13I 214 GGACC G^G[ACGT]CC 546 GGCCC DpnII 253 GATC ^GATC 370 GATC DsaI 276 CCACGG C^C[GA][CT]GG 355 CCGTGG EarI 63 GAAGAG CTCTTC(1/4) 493 GAAGAG EcoRII 311 CCTGG ^CC[AT]GG 543 CCTGG Hin1I 112 GGCGCC G[GA]^CG[CT]C 526 GACGTC Ksp632I 63 GAAGAG CTCTTC(1/4) 493 GAAGAG MboI 253 GATC ^GATC 370 GATC MluNI 23 TGGCCA TGG^CCA 273 TGGCCA MscI 23 TGGCCA TGG^CCA 273 TGGCCA MseI 248 TTAA T^TAA 407 TTAA MvaI 311 CCTGG CC^[AT]GG 543 CCTGG NdeII 253 GATC ^GATC 370 GATC NlaIII 165 CATG CATG^ 174 CATG Sau3AI 253 GATC ^GATC 370 GATC Sau96I 214 GGACC G^G[ACGT]CC 546 GGCCC SfaNI 391 GATGC GCATC(5/9) 566 GCATC Tru9I 248 TTAA T^TAA 407 TTAA Tsp509I 12 AATT ^AATT 18 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 24 enzymes) # ######################################### AciI AluI AlwI AlwNI BbvI BssKI Cac8I CfoI DdeI EaeI Fnu4HI HaeIII HapII HhaI HinP1I HpaII ItaI MboII MnlI MspI MwoI NlaIV ScrFI TseI