################################## # NO CUT ENZYMES ( 104 enzymes) # ################################## Acc65I AccIII AclI AflII AgeI AhdI Alw44I AocI Aor51HI ApaLI Asp718I AspEI AvaI AviII AvrII BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BsrBI BsrGI BssSI BstAPI BstBI Bsu36I CelII ClaI CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoRI EcoRV FseI FspI HgaI HindIII KasI KpnI KspI MfeI MluI MroI MunI NarI NheI NotI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SspBI StuI SwaI XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AatII 2160 GACGTC GACGT^C AcyI 2160 GACGTC G[GA]^CG[CT]C AlwNI 1370 CAGTTACTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 1401 GGGCCC GGGCC^C AscI 392 GGCGCGCC GG^CGCGCC AseI 1882 ATTAAT AT^TAAT AsnI 1882 ATTAAT AT^TAAT AspHI 1359 GAGCAC G[AT]GC[AT]^C AspI 2554 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 2400 TGGCCA TGG^CCA BcnI 2391 CCGGG CC^[GC]GG BglI 14 GCCCCTCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaBI 2530 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 2160 GACGTC G[GA]^CG[CT]C BseRI 2063 GAGGAG GAGGAG(10/8) BsgI 2028 CTGCAC GTGCAG(16/14) BsiHKAI 1359 GAGCAC G[AT]GC[AT]^C BspMI 858 GCAGGT ACCTGC(4/8) BsrFI 44 GCCGGC [GA]^CCGG[CT] BssHII 393 GCGCGC G^CGCGC Bst1107I 660 GTATAC GTA^TAC BstEII 1551 GGTCACC G^GT[ACGT]ACC BstPI 1551 GGTCACC G^GT[ACGT]ACC BstYI 2043 AGATCC [GA]^GATC[CT] BstZ17I 660 GTATAC GTA^TAC Cfr10I 44 GCCGGC [GA]^CCGG[CT] EaeI 2400 TGGCCA [CT]^GGCC[GA] EcoNI 465 CCTTCAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 1551 GGTCACC G^GT[ACGT]ACC FauI 11 CCCGC CCCGC(4/6) HaeII 2284 GGCGCT [GA]GCGC^[CT] Hin1I 2160 GACGTC G[GA]^CG[CT]C HpaI 154 GTTAAC GTT^AAC MamI 2530 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 2043 AGATCC [GA]^GATC[CT] MluNI 2400 TGGCCA TGG^CCA MscI 2400 TGGCCA TGG^CCA MspA1I 176 CAGCTG C[AC]G^C[GT]G NaeI 44 GCCGGC GCC^GGC NciI 2391 CCGGG CC^[GC]GG NcoI 1809 CCATGG C^CATGG NgoMIV 44 GCCGGC G^CCGGC NruI 65 TCGCGA TCG^CGA PflFI 2554 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 2419 GACTC GAGTC(4/5) PshAI 1825 GACTTTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 1882 ATTAAT AT^TAAT PvuII 176 CAGCTG CAG^CTG SbfI 2423 CCTGCAGG CCTGCA^GG ScaI 842 AGTACT AGT^ACT Sse8387I 2423 CCTGCAGG CCTGCA^GG Tth111I 2554 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoII 2043 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AccI 660 GTATAC GT^[AC][GT]AC 1001 GTCTAC AccII 66 CGCG CG^CG 394 CGCG AflIII 111 ACATGT A^C[GA][CT]GT 1672 ACATGT BanI 861 GGTGCC G^G[CT][GA]CC 2308 GGCACC BclI 908 TGATCA T^GATCA 1745 TGATCA BsaI 1612 GAGACC GGTCTC(1/5) 2344 GAGACC BspLU11I 111 ACATGT A^CATGT 1672 ACATGT BstUI 66 CGCG CG^CG 394 CGCG BstXI 1540 CCAACAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2348 CCATCGAAATGG DraI 1185 TTTAAA TTT^AAA 1210 TTTAAA DraIII 2000 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG 2033 CACATCGTG DsaI 1809 CCATGG C^C[GA][CT]GG 2312 CCGTGG EcoT22I 889 ATGCAT ATGCA^T 2691 ATGCAT FbaI 908 TGATCA T^GATCA 1745 TGATCA HincII 154 GTTAAC GT[CT]^[GA]AC 532 GTTGAC HindII 154 GTTAAC GT[CT]^[GA]AC 532 GTTGAC MvnI 66 CGCG CG^CG 394 CGCG NdeI 1484 CATATG CA^TATG 1934 CATATG NsiI 889 ATGCAT ATGCA^T 2691 ATGCAT PstI 2424 CTGCAG CTGCA^G 2454 CTGCAG SphI 2544 GCATGC GCATG^C 2689 GCATGC TaqI 427 TCGA T^CGA 2351 TCGA TthHB8I 427 TCGA T^CGA 2351 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 78 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI Asp700I AvaII BanII BbvI BfaI BmyI BpmI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MspI MvaI MwoI NdeII NlaIII NlaIV NspI PflMI PpuMI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SspI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Van91I XmnI