(Circular) (Six-base) MAPSORT of: pFN21AE2542.seq Check: 4269 from: 1 to: 7408 pFN21AE2542 7408 bp With 182 enzymes: SgfI * January 27, 2015 16:55 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2854 2854 Size: 7408 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4169 5218 278 Size: 53 83 186 936 2633 1049 2468 Fragments arranged by size: 2633 2468 1049 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2666 3007 4751 1937 Size: 729 341 1744 4594 Fragments arranged by size: 4594 1744 729 341 Acc65I G'GTAC_C Cuts at: 4729 4729 Size: 7408 AclI AA'CG_TT Cuts at: 1563 5535 5908 1563 Size: 3972 373 3063 Fragments arranged by size: 3972 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2864 3837 5471 6855 7123 1632 Size: 1232 973 1634 1384 268 1917 Fragments arranged by size: 1917 1634 1384 1232 973 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7211 AflIII A'CryG_T Cuts at: 1203 2116 3677 6307 7284 1203 Size: 913 1561 2630 977 1327 Fragments arranged by size: 2630 1561 1327 977 913 AgeI A'CCGG_T Cuts at: 6239 6239 Size: 7408 AhdI GACnn_n'nnGTC Cuts at: 6137 6137 Size: 7408 AleI CACnn'nnGTG Cuts at: 1166 2786 4448 1166 Size: 1620 1662 4126 Fragments arranged by size: 4126 1662 1620 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4136 4711 1184 Size: 2952 575 3881 Fragments arranged by size: 3881 2952 575 AlwNI CAG_nnn'CTG Cuts at: 3380 5151 6723 3380 Size: 1771 1572 4065 Fragments arranged by size: 4065 1771 1572 ApaI G_GGCC'C Cuts at: 3410 3410 Size: 7408 ApaLI G'TGCA_C Cuts at: 5465 6621 5465 Size: 1156 6252 ApoI r'AATT_y Cuts at: 1493 2220 2554 4649 4673 4717 4943 5095 7204 Size: 727 334 2095 24 44 226 152 2109 Cuts at: 7204 1493 Size: 1697 Fragments arranged by size: 2109 2095 1697 727 334 226 152 44 24 AscI GG'CGCG_CC Cuts at: 2398 2398 Size: 7408 AseI AT'TA_AT Cuts at: 160 1032 3888 5962 160 Size: 872 2856 2074 1606 Fragments arranged by size: 2856 2074 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7408 AvaI C'yCGr_G Cuts at: 1870 1943 4733 1870 Size: 73 2790 4545 Fragments arranged by size: 4545 2790 73 BamHI G'GATC_C Cuts at: 4738 5105 4738 Size: 367 7041 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2866 4313 4729 6184 618 Size: 325 217 627 1079 1447 416 1455 1842 Fragments arranged by size: 1842 1455 1447 1079 627 416 325 217 BanII G_rGCy'C Cuts at: 1391 2044 2419 3410 4671 4727 1391 Size: 653 375 991 1261 56 4072 Fragments arranged by size: 4072 1261 991 653 375 56 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6437 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5599 5633 1903 Size: 34 3662 34 3678 Fragments arranged by size: 3678 3662 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5300 6510 5300 Size: 1210 6198 BclI T'GATC_A Cuts at: 1548 2913 3750 1548 Size: 1365 837 5206 Fragments arranged by size: 5206 1365 837 BfrBI ATG'CAT Cuts at: 2896 4698 2896 Size: 1802 5606 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2025 6019 Size: 107 122 71 631 605 337 16 3994 Cuts at: 6019 136 Size: 1525 Fragments arranged by size: 3994 1525 631 605 337 122 107 71 16 BglII A'GATC_T Cuts at: 7403 7403 Size: 7408 BlpI GC'TnA_GC Cuts at: 4825 4825 Size: 7408 Bme1580I G_kGCm'C Cuts at: 1790 2783 3410 5469 6625 1790 Size: 993 627 2059 1156 2573 Fragments arranged by size: 2573 2059 1156 993 627 BmgBI CAC'GTC Cuts at: 1318 3712 3905 1318 Size: 2394 193 4821 Fragments arranged by size: 4821 2394 193 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3701 5139 6097 Size: 416 53 83 399 24 2284 1438 958 Cuts at: 6097 442 Size: 1753 Fragments arranged by size: 2284 1753 1438 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7408 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2553 2640 2655 3190 3860 4260 6068 1239 Size: 1314 87 15 535 670 400 1808 2579 Fragments arranged by size: 2579 1808 1314 670 535 400 87 15 Bpu10I CC'TnA_GC Cuts at: 4299 4299 Size: 7408 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5533 6398 6696 5533 Size: 865 298 6245 Fragments arranged by size: 6245 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3611 4343 6071 882 Size: 617 2112 732 1728 2219 Fragments arranged by size: 2219 2112 1728 732 617 BsaAI yAC'GTr Cuts at: 493 1204 5199 493 Size: 711 3995 2702 Fragments arranged by size: 3995 2702 711 BsaBI GATnn'nnATC Cuts at: 4539 7402 4539 Size: 2863 4545 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4166 5215 5597 6232 Size: 53 83 186 936 2633 1049 382 635 Cuts at: 6232 275 Size: 1451 Fragments arranged by size: 2633 1451 1049 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5841 6239 6513 6660 7071 5841 Size: 398 274 147 411 6178 Fragments arranged by size: 6178 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3664 3694 4219 4249 1178 Size: 30 165 30 2261 30 525 30 4337 Fragments arranged by size: 4337 2261 525 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 4083 1186 Size: 2897 4511 BseYI C'CCAG_C Cuts at: 4203 4601 6611 7107 4203 Size: 398 2010 496 4504 Fragments arranged by size: 4504 2010 496 398 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4018 1163 Size: 573 2282 4553 Fragments arranged by size: 4553 2282 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4858 5619 6647 1540 Size: 275 186 2857 761 1028 2301 Fragments arranged by size: 2857 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3368 5469 5554 6625 3368 Size: 2101 85 1071 4151 Fragments arranged by size: 4151 2101 1071 85 BsmI GAATG_Cn' Cuts at: 4919 5012 4919 Size: 93 7315 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2044 2419 2783 3368 3410 4671 4727 Size: 399 254 375 364 585 42 1261 56 Cuts at: 4727 5469 5554 6625 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1261 1071 742 585 399 375 364 254 85 56 42 BspEI T'CCGG_A Cuts at: 7071 7071 Size: 7408 BspHI T'CATG_A Cuts at: 5297 7027 5297 Size: 1730 5678 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2854 4763 844 Size: 2010 1909 3489 Fragments arranged by size: 3489 2010 1909 BsrBI CCG'CTC Cuts at: 1394 1823 5295 1394 Size: 429 3472 3507 Fragments arranged by size: 3507 3472 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3205 3481 4118 5903 6077 65 Size: 1183 1957 276 637 1785 174 1396 Fragments arranged by size: 1957 1785 1396 1183 637 276 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2049 6052 6239 1700 Size: 110 239 4003 187 2869 Fragments arranged by size: 4003 2869 239 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7408 BssHII G'CGCG_C Cuts at: 1925 2398 1925 Size: 473 6935 BssSI C'ACGA_G Cuts at: 5468 6480 5468 Size: 1012 6396 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7408 BstBI TT'CG_AA Cuts at: 6212 6212 Size: 7408 BstEII G'GTnAC_C Cuts at: 1184 3556 1184 Size: 2372 5036 BstXI CCAn_nnnn'nTGG Cuts at: 3552 4360 3552 Size: 808 6600 BstYI r'GATC_y Cuts at: 1972 4048 4738 5105 5490 5507 6959 7045 7057 Size: 2076 690 367 385 17 1452 86 12 Cuts at: 7057 7403 1972 Size: 346 1977 Fragments arranged by size: 2076 1977 1452 690 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2667 2667 Size: 7408 BtgI C'CryG_G Cuts at: 513 1065 2004 3814 4317 513 Size: 552 939 1810 503 3604 Fragments arranged by size: 3604 1810 939 552 503 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6669 Fragments arranged by size: 6669 683 56 BtsI GCAGTG_nn' Cuts at: 2681 3520 4627 4924 5718 5738 2681 Size: 839 1107 297 794 20 4351 Fragments arranged by size: 4351 1107 839 794 297 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7408 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7373 DraI TTT'AAA Cuts at: 3192 3217 4712 3192 Size: 25 1495 5888 Fragments arranged by size: 5888 1495 25 DraIII CAC_nnn'GTG Cuts at: 4010 4043 4010 Size: 33 7375 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6415 809 Size: 507 5099 1802 Fragments arranged by size: 5099 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4405 4855 5744 Size: 54 1425 50 275 18 2575 450 889 Cuts at: 5744 7373 8 Size: 1629 43 Fragments arranged by size: 2575 1629 1425 889 450 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4855 1537 Size: 275 3043 4090 Fragments arranged by size: 4090 3043 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2270 4582 4697 5338 1353 Size: 917 2312 115 641 3423 Fragments arranged by size: 3423 2312 917 641 115 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5995 6369 6515 387 Size: 1064 138 4406 374 146 1280 Fragments arranged by size: 4406 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2553 2640 2655 2864 3190 3837 3860 Size: 393 921 87 15 209 326 647 23 Cuts at: 3860 4260 5471 6068 6855 7123 1239 Size: 400 1211 597 787 268 1524 Fragments arranged by size: 1524 1211 921 787 647 597 400 393 326 268 209 87 23 15 EcoNI CCTnn'n_nnAGG Cuts at: 2474 2474 Size: 7408 EcoO109I rG'GnC_Cy Cuts at: 2359 2453 2528 3406 3407 2359 Size: 94 75 878 1 6360 Fragments arranged by size: 6360 878 94 75 1 EcoRI G'AATT_C Cuts at: 4717 5095 4717 Size: 378 7030 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3269 4661 3269 Size: 1392 6016 FspI TGC'GCA Cuts at: 5914 5914 Size: 7408 HaeII r_GCGC'y Cuts at: 1678 4293 6555 1678 Size: 2615 2262 2531 Fragments arranged by size: 2615 2531 2262 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4314 4346 6049 6081 6123 Size: 32 135 32 2742 32 1703 32 42 Cuts at: 6123 6155 1373 Size: 32 2626 Fragments arranged by size: 2742 2626 1703 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2161 2539 4752 4998 669 Size: 988 281 223 378 2213 246 3079 Fragments arranged by size: 3079 2213 988 378 281 246 223 HindIII A'AGCT_T Cuts at: 748 748 Size: 7408 HpaI GTT'AAC Cuts at: 2161 4998 2161 Size: 2837 4571 KpnI G_GTAC'C Cuts at: 4733 4733 Size: 7408 MfeI C'AATT_G Cuts at: 5007 5007 Size: 7408 MluI A'CGCG_T Cuts at: 7284 7284 Size: 7408 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2325 2924 3569 6522 6706 1305 Size: 1020 599 645 2953 184 2007 Fragments arranged by size: 2953 2007 1020 645 599 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4407 10 Size: 54 1425 343 2575 3011 Fragments arranged by size: 3011 2575 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2202 2786 4358 4448 4548 5366 5725 Size: 648 1036 584 1572 90 100 818 359 Cuts at: 5725 5884 518 Size: 159 2042 Fragments arranged by size: 2042 1572 1036 818 648 584 359 159 100 90 MspA1I CmG'CkG Cuts at: 1606 2183 4824 5501 6649 6894 1606 Size: 577 2641 677 1148 245 2120 Fragments arranged by size: 2641 2120 1148 677 577 245 NaeI GCC'GGC Cuts at: 2051 2051 Size: 7408 NcoI C'CATG_G Cuts at: 513 1065 2004 3814 513 Size: 552 939 1810 4107 Fragments arranged by size: 4107 1810 939 552 NdeI CA'TA_TG Cuts at: 387 3490 3940 387 Size: 3103 450 3855 Fragments arranged by size: 3855 3103 450 NgoMIV G'CCGG_C Cuts at: 2049 2049 Size: 7408 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7408 NotI GC'GGCC_GC Cuts at: 4855 4855 Size: 7408 NruI TCG'CGA Cuts at: 2072 2072 Size: 7408 NsiI A_TGCA'T Cuts at: 2898 4700 2898 Size: 1802 5606 NspI r_CATG'y Cuts at: 1132 1229 2120 3681 3979 4553 4698 4766 6311 Size: 97 891 1561 298 574 145 68 1545 Cuts at: 6311 1132 Size: 2229 Fragments arranged by size: 2229 1561 1545 891 574 298 145 97 68 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7408 PciI A'CATG_T Cuts at: 2116 3677 6307 2116 Size: 1561 2630 3217 Fragments arranged by size: 3217 2630 1561 PflMI CCAn_nnn'nTGG Cuts at: 1108 2224 4058 4285 1108 Size: 1116 1834 227 4231 Fragments arranged by size: 4231 1834 1116 227 PfoI T'CCnGG_A Cuts at: 2030 2530 4237 2030 Size: 500 1707 5201 Fragments arranged by size: 5201 1707 500 PmeI GTTT'AAAC Cuts at: 4712 4712 Size: 7408 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5477 5509 6990 7022 5477 Size: 32 1481 32 5863 Fragments arranged by size: 5863 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2359 2453 2528 2359 Size: 94 75 7239 Fragments arranged by size: 7239 94 75 PshAI GACnn'nnGTC Cuts at: 1613 3834 1613 Size: 2221 5187 PsiI TTA'TAA Cuts at: 4978 4978 Size: 7408 PspOMI G'GGCC_C Cuts at: 3406 3406 Size: 7408 PstI C_TGCA'G Cuts at: 830 4433 4463 4760 830 Size: 3603 30 297 3478 Fragments arranged by size: 3603 3478 297 30 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7408 PvuII CAG'CTG Cuts at: 2183 2183 Size: 7408 SalI G'TCGA_C Cuts at: 1936 4750 1936 Size: 2814 4594 SbfI CC_TGCA'GG Cuts at: 4433 4760 4433 Size: 327 7081 ScaI AGT'ACT Cuts at: 2849 5656 7368 2849 Size: 2807 1712 2889 Fragments arranged by size: 2889 2807 1712 SfcI C'TryA_G Cuts at: 826 1045 2604 2904 3008 4429 4459 4608 4756 Size: 219 1559 300 104 1421 30 149 148 Cuts at: 4756 5891 6572 6763 826 Size: 1135 681 191 1471 Fragments arranged by size: 1559 1471 1421 1135 681 300 219 191 149 148 104 30 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7408 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7408 SmaI CCC'GGG Cuts at: 1872 4735 1872 Size: 2863 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 5512 6413 6675 820 Size: 197 926 3569 901 262 1553 Fragments arranged by size: 3569 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5199 493 Size: 4706 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7408 SphI G_CATG'C Cuts at: 1132 4553 4698 4766 1132 Size: 3421 145 68 3774 Fragments arranged by size: 3774 3421 145 68 SspI AAT'ATT Cuts at: 5 52 2082 2331 3643 4104 5332 5 Size: 47 2030 249 1312 461 1228 2081 Fragments arranged by size: 2081 2030 1312 1228 461 249 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7408 StyI C'CwwG_G Cuts at: 513 1065 2004 2448 3664 3814 4052 4847 513 Size: 552 939 444 1216 150 238 795 3074 Fragments arranged by size: 3074 1216 939 795 552 444 238 150 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2893 5435 5452 5605 5790 6235 325 Size: 1255 1313 2542 17 153 185 445 1498 Fragments arranged by size: 2542 1498 1313 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2594 2847 2977 Size: 274 80 33 51 1443 617 253 130 Cuts at: 2977 3859 4072 5654 7366 96 Size: 882 213 1582 1712 138 Fragments arranged by size: 1712 1582 1443 882 617 274 253 213 138 130 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4048 4080 4219 4251 7368 7400 4048 Size: 32 139 32 3117 32 4056 Fragments arranged by size: 4056 3117 139 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4562 1316 Size: 3246 4162 XbaI T'CTAG_A Cuts at: 4744 4744 Size: 7408 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7408 XmaI C'CCGG_G Cuts at: 1870 4733 1870 Size: 2863 4545 XmnI GAAnn'nnTTC Cuts at: 2388 3249 4268 5537 2388 Size: 861 1019 1269 4259 Fragments arranged by size: 4259 1269 1019 861 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4167 5216 276 Size: 53 83 186 936 2633 1049 2468 Fragments arranged by size: 2633 2468 1049 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI Bsu36I EcoICRI EcoRV FseI FspAI KasI NarI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI