(Linear) (Six-base) MAPSORT of: KIEE2542.seq Check: 5481 from: 1 to: 2703 KIEE2542.orf With 182 enzymes: SgfI * November 7, 2014 16:44 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 849 2703 Size: 849 1854 AatII G_ACGT'C Cuts at: 0 2164 2703 Size: 2164 539 AccI GT'mk_AC Cuts at: 0 661 1002 2703 Size: 661 341 1701 Fragments arranged by size: 1701 661 341 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 859 1832 2703 Size: 859 973 871 Fragments arranged by size: 973 871 859 AflIII A'CryG_T Cuts at: 0 111 1672 2703 Size: 111 1561 1031 Fragments arranged by size: 1561 1031 111 AleI CACnn'nnGTG Cuts at: 0 781 2443 2703 Size: 781 1662 260 Fragments arranged by size: 1662 781 260 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2131 2703 Size: 2131 572 AlwNI CAG_nnn'CTG Cuts at: 0 1375 2703 Size: 1375 1328 ApaI G_GGCC'C Cuts at: 0 1405 2703 Size: 1405 1298 ApoI r'AATT_y Cuts at: 0 215 549 2644 2668 2703 Size: 215 334 2095 24 35 Fragments arranged by size: 2095 334 215 35 24 AscI GG'CGCG_CC Cuts at: 0 393 2703 Size: 393 2310 AseI AT'TA_AT Cuts at: 0 1883 2703 Size: 1883 820 BanI G'GyrC_C Cuts at: 0 861 2308 2703 Size: 861 1447 395 Fragments arranged by size: 1447 861 395 BanII G_rGCy'C Cuts at: 0 39 414 1405 2666 2703 Size: 39 375 991 1261 37 Fragments arranged by size: 1261 991 375 39 37 BclI T'GATC_A Cuts at: 0 908 1745 2703 Size: 908 837 958 Fragments arranged by size: 958 908 837 BfrBI ATG'CAT Cuts at: 0 891 2693 2703 Size: 891 1802 10 Fragments arranged by size: 1802 891 10 BglI GCCn_nnn'nGGC Cuts at: 0 20 2703 Size: 20 2683 Bme1580I G_kGCm'C Cuts at: 0 778 1405 2703 Size: 778 627 1298 Fragments arranged by size: 1298 778 627 BmgBI CAC'GTC Cuts at: 0 1707 1900 2703 Size: 1707 193 803 Fragments arranged by size: 1707 803 193 BmrI ACTGGGnnnn_n' Cuts at: 0 1696 2703 Size: 1696 1007 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 548 635 650 1185 1855 2255 2703 Size: 548 87 15 535 670 400 448 Fragments arranged by size: 670 548 535 448 400 87 15 Bpu10I CC'TnA_GC Cuts at: 0 2294 2703 Size: 2294 409 BsaI GGTCTCn'nnnn_ Cuts at: 0 1606 2338 2703 Size: 1606 732 365 Fragments arranged by size: 1606 732 365 BsaBI GATnn'nnATC Cuts at: 0 2534 2703 Size: 2534 169 BsaHI Gr'CG_yC Cuts at: 0 2161 2703 Size: 2161 542 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1659 1689 2214 2244 2703 Size: 1659 30 525 30 459 Fragments arranged by size: 1659 525 459 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 2078 2703 Size: 2078 625 BseYI C'CCAG_C Cuts at: 0 2198 2596 2703 Size: 2198 398 107 Fragments arranged by size: 2198 398 107 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2013 2703 Size: 2013 690 BsiHKAI G_wGCw'C Cuts at: 0 1363 2703 Size: 1363 1340 Bsp1286I G_dGCh'C Cuts at: 0 39 414 778 1363 1405 2666 2703 Size: 39 375 364 585 42 1261 37 Fragments arranged by size: 1261 585 375 364 42 39 37 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 849 2703 Size: 849 1854 BsrDI GCAATG_nn' Cuts at: 0 1200 1476 2113 2703 Size: 1200 276 637 590 Fragments arranged by size: 1200 637 590 276 BsrFI r'CCGG_y Cuts at: 0 44 2703 Size: 44 2659 BssHII G'CGCG_C Cuts at: 0 393 2703 Size: 393 2310 BstEII G'GTnAC_C Cuts at: 0 1551 2703 Size: 1551 1152 BstXI CCAn_nnnn'nTGG Cuts at: 0 1547 2355 2703 Size: 1547 808 348 Fragments arranged by size: 1547 808 348 BstYI r'GATC_y Cuts at: 0 2043 2703 Size: 2043 660 BstZ17I GTA'TAC Cuts at: 0 662 2703 Size: 662 2041 BtgI C'CryG_G Cuts at: 0 1809 2312 2703 Size: 1809 503 391 Fragments arranged by size: 1809 503 391 BtsI GCAGTG_nn' Cuts at: 0 676 1515 2622 2703 Size: 676 839 1107 81 Fragments arranged by size: 1107 839 676 81 DraI TTT'AAA Cuts at: 0 1187 1212 2703 Size: 1187 25 1491 Fragments arranged by size: 1491 1187 25 DraIII CAC_nnn'GTG Cuts at: 0 2005 2038 2703 Size: 2005 33 665 Fragments arranged by size: 2005 665 33 EaeI y'GGCC_r Cuts at: 0 2400 2703 Size: 2400 303 EarI CTCTTCn'nnn_ Cuts at: 0 265 2577 2692 2703 Size: 265 2312 115 11 Fragments arranged by size: 2312 265 115 11 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 548 635 650 859 1185 1832 1855 2255 Size: 548 87 15 209 326 647 23 400 Cuts at: 2255 2703 Size: 448 Fragments arranged by size: 647 548 448 400 326 209 87 23 15 EcoNI CCTnn'n_nnAGG Cuts at: 0 469 2703 Size: 469 2234 EcoO109I rG'GnC_Cy Cuts at: 0 354 448 523 1401 1402 2703 Size: 354 94 75 878 1 1301 Fragments arranged by size: 1301 878 354 94 75 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1264 2656 2703 Size: 1264 1392 47 Fragments arranged by size: 1392 1264 47 HaeII r_GCGC'y Cuts at: 0 2288 2703 Size: 2288 415 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 2309 2341 2703 Size: 2309 32 362 Fragments arranged by size: 2309 362 32 HincII GTy'rAC Cuts at: 0 156 534 2703 Size: 156 378 2169 Fragments arranged by size: 2169 378 156 HpaI GTT'AAC Cuts at: 0 156 2703 Size: 156 2547 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 320 919 1564 2703 Size: 320 599 645 1139 Fragments arranged by size: 1139 645 599 320 MscI TGG'CCA Cuts at: 0 2402 2703 Size: 2402 301 MslI CAynn'nnrTG Cuts at: 0 197 781 2353 2443 2543 2703 Size: 197 584 1572 90 100 160 Fragments arranged by size: 1572 584 197 160 100 90 MspA1I CmG'CkG Cuts at: 0 178 2703 Size: 178 2525 NaeI GCC'GGC Cuts at: 0 46 2703 Size: 46 2657 NcoI C'CATG_G Cuts at: 0 1809 2703 Size: 1809 894 NdeI CA'TA_TG Cuts at: 0 1485 1935 2703 Size: 1485 450 768 Fragments arranged by size: 1485 768 450 NgoMIV G'CCGG_C Cuts at: 0 44 2703 Size: 44 2659 NruI TCG'CGA Cuts at: 0 67 2703 Size: 67 2636 NsiI A_TGCA'T Cuts at: 0 893 2695 2703 Size: 893 1802 8 Fragments arranged by size: 1802 893 8 NspI r_CATG'y Cuts at: 0 115 1676 1974 2548 2693 2703 Size: 115 1561 298 574 145 10 Fragments arranged by size: 1561 574 298 145 115 10 PciI A'CATG_T Cuts at: 0 111 1672 2703 Size: 111 1561 1031 Fragments arranged by size: 1561 1031 111 PflMI CCAn_nnn'nTGG Cuts at: 0 219 2053 2280 2703 Size: 219 1834 227 423 Fragments arranged by size: 1834 423 227 219 PfoI T'CCnGG_A Cuts at: 0 25 525 2232 2703 Size: 25 500 1707 471 Fragments arranged by size: 1707 500 471 25 PpuMI rG'GwC_Cy Cuts at: 0 354 448 523 2703 Size: 354 94 75 2180 Fragments arranged by size: 2180 354 94 75 PshAI GACnn'nnGTC Cuts at: 0 1829 2703 Size: 1829 874 PspOMI G'GGCC_C Cuts at: 0 1401 2703 Size: 1401 1302 PstI C_TGCA'G Cuts at: 0 2428 2458 2703 Size: 2428 30 245 Fragments arranged by size: 2428 245 30 PvuII CAG'CTG Cuts at: 0 178 2703 Size: 178 2525 SbfI CC_TGCA'GG Cuts at: 0 2428 2703 Size: 2428 275 ScaI AGT'ACT Cuts at: 0 844 2703 Size: 844 1859 SfcI C'TryA_G Cuts at: 0 599 899 1003 2424 2454 2603 2703 Size: 599 300 104 1421 30 149 100 Fragments arranged by size: 1421 599 300 149 104 100 30 SphI G_CATG'C Cuts at: 0 2548 2693 2703 Size: 2548 145 10 Fragments arranged by size: 2548 145 10 SspI AAT'ATT Cuts at: 0 77 326 1638 2099 2703 Size: 77 249 1312 461 604 Fragments arranged by size: 1312 604 461 249 77 StyI C'CwwG_G Cuts at: 0 443 1659 1809 2047 2703 Size: 443 1216 150 238 656 Fragments arranged by size: 1216 656 443 238 150 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 888 2703 Size: 888 1815 TatI w'GTAC_w Cuts at: 0 589 842 972 1854 2067 2703 Size: 589 253 130 882 213 636 Fragments arranged by size: 882 636 589 253 213 130 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2043 2075 2214 2246 2703 Size: 2043 32 139 32 457 Fragments arranged by size: 2043 457 139 32 32 Tth111I GACn'n_nGTC Cuts at: 0 2557 2703 Size: 2557 146 XmnI GAAnn'nnTTC Cuts at: 0 383 1244 2263 2703 Size: 383 861 1019 440 Fragments arranged by size: 1019 861 440 383 ZraI GAC'GTC Cuts at: 0 2162 2703 Size: 2162 541 Enzymes that do cut: AarI AatII AccI AcuI AflIII AleI AlfI AlwNI ApaI ApoI AscI AseI BanI BanII BclI BfrBI BglI Bme1580I BmgBI BmrI BpmI Bpu10I BsaI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrDI BsrFI BssHII BstEII BstXI BstYI BstZ17I BtgI BtsI DraI DraIII EaeI EarI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HincII HpaI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NruI NsiI NspI PciI PflMI PfoI PpuMI PshAI PspOMI PstI PvuII SbfI ScaI SfcI SphI SspI StyI TaqII TatI TstI Tth111I XmnI ZraI Enzymes that do not cut: Acc65I AclI AfeI AflII AgeI AhdI AloI ApaLI AsiSI AvaI AvrII BaeI BamHI BbeI BbsI BbvCI BcgI BciVI BglII BlpI BmtI BplI BpuEI BsaAI BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrGI BssSI BstAPI BstBI Bsu36I BtgZI ClaI CspCI DrdI EagI EciI EcoICRI EcoRI EcoRV FseI FspI FspAI HindIII KasI KpnI MfeI MluI NarI NheI NotI PacI PasI PmeI PmlI PpiI PsiI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI StuI SwaI XbaI XcmI XhoI XmaI