Restriction map for pFN21AE2542
 (Linear) (Six-base) MAPSORT of: KIEE2542.seq  Check: 5481  from: 1  to: 2703



KIEE2542.orf

 With 182 enzymes: SgfI * 

                        November 7, 2014 16:44  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     849    2703 
   Size:        849    1854
 
AatII G_ACGT'C

Cuts at:      0    2164    2703 
   Size:       2164     539
 
AccI GT'mk_AC

Cuts at:      0     661    1002    2703 
   Size:        661     341    1701

  Fragments arranged by size: 

                1701     661     341
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     859    1832    2703 
   Size:        859     973     871

  Fragments arranged by size: 

                 973     871     859
 
AflIII A'CryG_T

Cuts at:      0     111    1672    2703 
   Size:        111    1561    1031

  Fragments arranged by size: 

                1561    1031     111
 
AleI CACnn'nnGTG

Cuts at:      0     781    2443    2703 
   Size:        781    1662     260

  Fragments arranged by size: 

                1662     781     260
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2131    2703 
   Size:       2131     572
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1375    2703 
   Size:       1375    1328
 
ApaI G_GGCC'C

Cuts at:      0    1405    2703 
   Size:       1405    1298
 
ApoI r'AATT_y

Cuts at:      0     215     549    2644    2668    2703 
   Size:        215     334    2095      24      35

  Fragments arranged by size: 

                2095     334     215      35      24
 
AscI GG'CGCG_CC

Cuts at:      0     393    2703 
   Size:        393    2310
 
AseI AT'TA_AT

Cuts at:      0    1883    2703 
   Size:       1883     820
 
BanI G'GyrC_C

Cuts at:      0     861    2308    2703 
   Size:        861    1447     395

  Fragments arranged by size: 

                1447     861     395
 
BanII G_rGCy'C

Cuts at:      0      39     414    1405    2666    2703 
   Size:         39     375     991    1261      37

  Fragments arranged by size: 

                1261     991     375      39      37
 
BclI T'GATC_A

Cuts at:      0     908    1745    2703 
   Size:        908     837     958

  Fragments arranged by size: 

                 958     908     837
 
BfrBI ATG'CAT

Cuts at:      0     891    2693    2703 
   Size:        891    1802      10

  Fragments arranged by size: 

                1802     891      10
 
BglI GCCn_nnn'nGGC

Cuts at:      0      20    2703 
   Size:         20    2683
 
Bme1580I G_kGCm'C

Cuts at:      0     778    1405    2703 
   Size:        778     627    1298

  Fragments arranged by size: 

                1298     778     627
 
BmgBI CAC'GTC

Cuts at:      0    1707    1900    2703 
   Size:       1707     193     803

  Fragments arranged by size: 

                1707     803     193
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1696    2703 
   Size:       1696    1007
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     548     635     650    1185    1855    2255    2703 
   Size:        548      87      15     535     670     400     448

  Fragments arranged by size: 

                 670     548     535     448     400      87      15
 
Bpu10I CC'TnA_GC

Cuts at:      0    2294    2703 
   Size:       2294     409
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1606    2338    2703 
   Size:       1606     732     365

  Fragments arranged by size: 

                1606     732     365
 
BsaBI GATnn'nnATC

Cuts at:      0    2534    2703 
   Size:       2534     169
 
BsaHI Gr'CG_yC

Cuts at:      0    2161    2703 
   Size:       2161     542
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1659    1689    2214    2244    2703 
   Size:       1659      30     525      30     459

  Fragments arranged by size: 

                1659     525     459      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    2078    2703 
   Size:       2078     625
 
BseYI C'CCAG_C

Cuts at:      0    2198    2596    2703 
   Size:       2198     398     107

  Fragments arranged by size: 

                2198     398     107
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2013    2703 
   Size:       2013     690
 
BsiHKAI G_wGCw'C

Cuts at:      0    1363    2703 
   Size:       1363    1340
 
Bsp1286I G_dGCh'C

Cuts at:      0      39     414     778    1363    1405    2666    2703 
   Size:         39     375     364     585      42    1261      37

  Fragments arranged by size: 

                1261     585     375     364      42      39      37
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     849    2703 
   Size:        849    1854
 
BsrDI GCAATG_nn'

Cuts at:      0    1200    1476    2113    2703 
   Size:       1200     276     637     590

  Fragments arranged by size: 

                1200     637     590     276
 
BsrFI r'CCGG_y

Cuts at:      0      44    2703 
   Size:         44    2659
 
BssHII G'CGCG_C

Cuts at:      0     393    2703 
   Size:        393    2310
 
BstEII G'GTnAC_C

Cuts at:      0    1551    2703 
   Size:       1551    1152
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1547    2355    2703 
   Size:       1547     808     348

  Fragments arranged by size: 

                1547     808     348
 
BstYI r'GATC_y

Cuts at:      0    2043    2703 
   Size:       2043     660
 
BstZ17I GTA'TAC

Cuts at:      0     662    2703 
   Size:        662    2041
 
BtgI C'CryG_G

Cuts at:      0    1809    2312    2703 
   Size:       1809     503     391

  Fragments arranged by size: 

                1809     503     391
 
BtsI GCAGTG_nn'

Cuts at:      0     676    1515    2622    2703 
   Size:        676     839    1107      81

  Fragments arranged by size: 

                1107     839     676      81
 
DraI TTT'AAA

Cuts at:      0    1187    1212    2703 
   Size:       1187      25    1491

  Fragments arranged by size: 

                1491    1187      25
 
DraIII CAC_nnn'GTG

Cuts at:      0    2005    2038    2703 
   Size:       2005      33     665

  Fragments arranged by size: 

                2005     665      33
 
EaeI y'GGCC_r

Cuts at:      0    2400    2703 
   Size:       2400     303
 
EarI CTCTTCn'nnn_

Cuts at:      0     265    2577    2692    2703 
   Size:        265    2312     115      11

  Fragments arranged by size: 

                2312     265     115      11
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     548     635     650     859    1185    1832    1855    2255 
   Size:        548      87      15     209     326     647      23     400

Cuts at:   2255    2703 
   Size:        448

  Fragments arranged by size: 

                 647     548     448     400     326     209      87      23
                  15
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     469    2703 
   Size:        469    2234
 
EcoO109I rG'GnC_Cy

Cuts at:      0     354     448     523    1401    1402    2703 
   Size:        354      94      75     878       1    1301

  Fragments arranged by size: 

                1301     878     354      94      75       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1264    2656    2703 
   Size:       1264    1392      47

  Fragments arranged by size: 

                1392    1264      47
 
HaeII r_GCGC'y

Cuts at:      0    2288    2703 
   Size:       2288     415
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    2309    2341    2703 
   Size:       2309      32     362

  Fragments arranged by size: 

                2309     362      32
 
HincII GTy'rAC

Cuts at:      0     156     534    2703 
   Size:        156     378    2169

  Fragments arranged by size: 

                2169     378     156
 
HpaI GTT'AAC

Cuts at:      0     156    2703 
   Size:        156    2547
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     320     919    1564    2703 
   Size:        320     599     645    1139

  Fragments arranged by size: 

                1139     645     599     320
 
MscI TGG'CCA

Cuts at:      0    2402    2703 
   Size:       2402     301
 
MslI CAynn'nnrTG

Cuts at:      0     197     781    2353    2443    2543    2703 
   Size:        197     584    1572      90     100     160

  Fragments arranged by size: 

                1572     584     197     160     100      90
 
MspA1I CmG'CkG

Cuts at:      0     178    2703 
   Size:        178    2525
 
NaeI GCC'GGC

Cuts at:      0      46    2703 
   Size:         46    2657
 
NcoI C'CATG_G

Cuts at:      0    1809    2703 
   Size:       1809     894
 
NdeI CA'TA_TG

Cuts at:      0    1485    1935    2703 
   Size:       1485     450     768

  Fragments arranged by size: 

                1485     768     450
 
NgoMIV G'CCGG_C

Cuts at:      0      44    2703 
   Size:         44    2659
 
NruI TCG'CGA

Cuts at:      0      67    2703 
   Size:         67    2636
 
NsiI A_TGCA'T

Cuts at:      0     893    2695    2703 
   Size:        893    1802       8

  Fragments arranged by size: 

                1802     893       8
 
NspI r_CATG'y

Cuts at:      0     115    1676    1974    2548    2693    2703 
   Size:        115    1561     298     574     145      10

  Fragments arranged by size: 

                1561     574     298     145     115      10
 
PciI A'CATG_T

Cuts at:      0     111    1672    2703 
   Size:        111    1561    1031

  Fragments arranged by size: 

                1561    1031     111
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     219    2053    2280    2703 
   Size:        219    1834     227     423

  Fragments arranged by size: 

                1834     423     227     219
 
PfoI T'CCnGG_A

Cuts at:      0      25     525    2232    2703 
   Size:         25     500    1707     471

  Fragments arranged by size: 

                1707     500     471      25
 
PpuMI rG'GwC_Cy

Cuts at:      0     354     448     523    2703 
   Size:        354      94      75    2180

  Fragments arranged by size: 

                2180     354      94      75
 
PshAI GACnn'nnGTC

Cuts at:      0    1829    2703 
   Size:       1829     874
 
PspOMI G'GGCC_C

Cuts at:      0    1401    2703 
   Size:       1401    1302
 
PstI C_TGCA'G

Cuts at:      0    2428    2458    2703 
   Size:       2428      30     245

  Fragments arranged by size: 

                2428     245      30
 
PvuII CAG'CTG

Cuts at:      0     178    2703 
   Size:        178    2525
 
SbfI CC_TGCA'GG

Cuts at:      0    2428    2703 
   Size:       2428     275
 
ScaI AGT'ACT

Cuts at:      0     844    2703 
   Size:        844    1859
 
SfcI C'TryA_G

Cuts at:      0     599     899    1003    2424    2454    2603    2703 
   Size:        599     300     104    1421      30     149     100

  Fragments arranged by size: 

                1421     599     300     149     104     100      30
 
SphI G_CATG'C

Cuts at:      0    2548    2693    2703 
   Size:       2548     145      10

  Fragments arranged by size: 

                2548     145      10
 
SspI AAT'ATT

Cuts at:      0      77     326    1638    2099    2703 
   Size:         77     249    1312     461     604

  Fragments arranged by size: 

                1312     604     461     249      77
 
StyI C'CwwG_G

Cuts at:      0     443    1659    1809    2047    2703 
   Size:        443    1216     150     238     656

  Fragments arranged by size: 

                1216     656     443     238     150
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     888    2703 
   Size:        888    1815
 
TatI w'GTAC_w

Cuts at:      0     589     842     972    1854    2067    2703 
   Size:        589     253     130     882     213     636

  Fragments arranged by size: 

                 882     636     589     253     213     130
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2043    2075    2214    2246    2703 
   Size:       2043      32     139      32     457

  Fragments arranged by size: 

                2043     457     139      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    2557    2703 
   Size:       2557     146
 
XmnI GAAnn'nnTTC

Cuts at:      0     383    1244    2263    2703 
   Size:        383     861    1019     440

  Fragments arranged by size: 

                1019     861     440     383
 
ZraI GAC'GTC

Cuts at:      0    2162    2703 
   Size:       2162     541
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI   AflIII     AleI     AlfI    AlwNI
     ApaI     ApoI     AscI     AseI     BanI    BanII     BclI    BfrBI
     BglI Bme1580I    BmgBI     BmrI     BpmI   Bpu10I     BsaI    BsaBI
    BsaHI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BspMI
    BsrDI    BsrFI   BssHII   BstEII    BstXI    BstYI  BstZ17I     BtgI
     BtsI     DraI   DraIII     EaeI     EarI  Eco57MI    EcoNI EcoO109I
     FalI    HaeII    Hin4I   HincII     HpaI     MmeI     MscI     MslI
   MspA1I     NaeI     NcoI     NdeI   NgoMIV     NruI     NsiI     NspI
     PciI    PflMI     PfoI    PpuMI    PshAI   PspOMI     PstI    PvuII
     SbfI     ScaI     SfcI     SphI     SspI     StyI    TaqII     TatI
     TstI  Tth111I     XmnI     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI     AfeI    AflII     AgeI     AhdI     AloI    ApaLI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI     BbsI    BbvCI
     BcgI    BciVI    BglII     BlpI     BmtI     BplI    BpuEI    BsaAI
    BsaWI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI
    BsrGI    BssSI   BstAPI    BstBI   Bsu36I    BtgZI     ClaI    CspCI
     DrdI     EagI     EciI  EcoICRI    EcoRI    EcoRV     FseI     FspI
    FspAI  HindIII     KasI     KpnI     MfeI     MluI     NarI     NheI
     NotI     PacI     PasI     PmeI     PmlI     PpiI     PsiI    PspXI
     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SrfI     StuI     SwaI     XbaI     XcmI     XhoI     XmaI
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