################################## # NO CUT ENZYMES ( 102 enzymes) # ################################## AatII Acc65I AclI AcyI AflIII AgeI AlwNI AocI Aor51HI ApaI AscI Asp718I AspI AviII AvrII BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BglI BlnI BsaAI BsaHI BsiEI BsiWI BsmBI Bsp1407I BspDI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstXI BstZ17I Bsu36I Cfr10I ClaI CpoI DraII DraIII DrdI EagI EclXI Eco47III Eco52I Eco81I EcoO109I EcoRV FbaI FseI FspI HaeII Hin1I KasI KpnI KspI MluI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NspV PacI PflFI PinAI PmaCI PmeI PmlI PpuMI Psp1406I PvuI RsrII SacI SacII SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI Sse8387I SspBI SwaI Tth111I XmaCI XmaI ################################### # ONE CUT ENZYMES ( 59 enzymes) # ################################### AccI 3 GTCGAC GT^[AC][GT]AC AccII 2714 CGCG CG^CG AccIII 4025 TCCGGA T^CCGGA AhdI 778 GACTGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 3209 GTGCAC G^TGCAC ApaLI 3209 GTGCAC G^TGCAC AseI 1483 ATTAAT AT^TAAT AsnI 1483 ATTAAT AT^TAAT AspEI 778 GACTGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 3209 GTGCAC G[AT]GC[AT]^C AvaI 2177 CTCGAG C^[CT]CG[GA]G AvaII 3285 GGACC G^G[AT]CC BalI 3239 TGGCCA TGG^CCA BamHI 57 GGATCC G^GATCC BsaI 1874 GAGACC GGTCTC(1/5) BsaWI 4025 TCCGGA [AT]^CCGG[AT] BseAI 4025 TCCGGA T^CCGGA BsgI 1356 GTGCAG GTGCAG(16/14) BsiHKAI 3209 GTGCAC G[AT]GC[AT]^C BsmI 424 GCATTC GAATGC(1/-1) BsoBI 2177 CTCGAG C^[CT]CG[GA]G BspEI 4025 TCCGGA T^CCGGA BspHI 1854 TCATGA T^CATGA BspMI 47 ACCTGC ACCTGC(4/8) BsrDI 2134 GCAATG GCAATG(2/0) BstAPI 553 GCATTGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 3340 GGTAACC G^GT[ACGT]ACC BstPI 3340 GGTAACC G^GT[ACGT]ACC BstUI 2714 CGCG CG^CG Cfr13I 3285 GGACC G^G[ACGT]CC DsaI 572 CCATGG C^C[GA][CT]GG EaeI 3239 TGGCCA [CT]^GGCC[GA] Eam1105I 778 GACTGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 1177 CCTTTTGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 3340 GGTAACC G^GT[ACGT]ACC EcoT22I 422 ATGCAT ATGCA^T HapII 4026 CCGG C^CGG HgaI 2748 GACGC GACGC(5/10) HpaI 3007 GTTAAC GTT^AAC HpaII 4026 CCGG C^CGG MluNI 3239 TGGCCA TGG^CCA MroI 4025 TCCGGA T^CCGGA MscI 3239 TGGCCA TGG^CCA MspI 4026 CCGG C^CGG MvnI 2714 CGCG CG^CG NcoI 572 CCATGG C^CATGG NsiI 422 ATGCAT ATGCA^T PaeR7I 2177 CTCGAG C^TCGAG PshAI 1846 GACAGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 1483 ATTAAT AT^TAAT PstI 536 CTGCAG CTGCA^G RcaI 1854 TCATGA T^CATGA SalI 3 GTCGAC G^TCGAC SapI 2878 GAAGAGC GCTCTTC(1/4) Sau96I 3285 GGACC G^G[ACGT]CC SphI 2837 GCATGC GCATG^C StuI 2718 AGGCCT AGG^CCT XbaI 1428 TCTAGA T^CTAGA XhoI 2177 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AflII 135 CTTAAG C^TTAAG 2950 CTTAAG BbsI 1570 GAAGAC GAAGAC(2/6) 2370 GAAGAC BfrI 135 CTTAAG C^TTAAG 2950 CTTAAG BglII 343 AGATCT A^GATCT 477 AGATCT BlpI 2476 GCTCAGC GC^T[ACGT]AGC 2542 GCTGAGC BmyI 521 GGGCAC G[AGT]GC[ACT]^C 3209 GTGCAC BpmI 2326 CTGGAG CTGGAG(16/14) 2923 CTGGAG Bpu1102I 2476 GCTCAGC GC^T[ACGT]AGC 2542 GCTGAGC BpuAI 1570 GAAGAC GAAGAC(2/6) 2370 GAAGAC Bsp1286I 521 GGGCAC G[AGT]GC[ACT]^C 3209 GTGCAC CelII 2476 GCTCAGC GC^T[ACGT]AGC 2542 GCTGAGC CfoI 2713 GCGC GCG^C 3190 GCGC FauI 54 GCGGG CCCGC(4/6) 2517 GCGGG HhaI 2713 GCGC GCG^C 3190 GCGC HinP1I 2713 GCGC G^CGC 3190 GCGC HincII 3 GTCGAC GT[CT]^[GA]AC 3007 GTTAAC HindII 3 GTCGAC GT[CT]^[GA]AC 3007 GTTAAC MaeII 2234 ACGT A^CGT 3524 ACGT NlaIV 57 GGATCC GG[ACGT]^[ACGT]CC 3636 GGAACC PflMI 567 CCATTCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3958 CCACCATCTGG SpeI 1093 ACTAGT A^CTAGT 4057 ACTAGT Van91I 567 CCATTCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3958 CCACCATCTGG XcmI 744 CCAAGGTGGTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3485 CCAATCCATCTATGG ######################################### # 3 OR MORE CUT ENZYMES ( 74 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI Asp700I BbvI BfaI BsaBI BsaJI BseRI BsiYI BslI BsmAI BsmFI BsrI BssKI BstF5I BstNI BstYI Cac8I DdeI DpnII DraI EarI EcoRI EcoRII EcoT14I Fnu4HI FokI HaeIII HindIII HinfI HphI ItaI Ksp632I MaeI MaeIII MamI MboI MboII MfeI MflI MnlI MseI MslI MspA1I MunI MvaI MwoI NdeII NlaIII NspI PleI PvuII RsaI Sau3AI ScrFI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI