(Circular) (Six-base) MAPSORT of: pFN21AE3460.seq Check: 2375 from: 1 to: 8767 pFN21AE3460 8767 bp With 182 enzymes: SgfI * March 28, 2012 14:05 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6577 278 Size: 53 83 186 936 5041 2468 Fragments arranged by size: 5041 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2009 6110 1937 Size: 72 4101 4594 Fragments arranged by size: 4594 4101 72 Acc65I G'GTAC_C Cuts at: 6088 6088 Size: 8767 AclI AA'CG_TT Cuts at: 1563 6894 7267 1563 Size: 5331 373 3063 Fragments arranged by size: 5331 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2244 2754 3210 4268 5216 5592 6830 8214 Size: 612 510 456 1058 948 376 1238 1384 Cuts at: 8214 8482 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1238 1058 948 612 510 456 376 268 AflII C'TTAA_G Cuts at: 820 1017 2140 4955 820 Size: 197 1123 2815 4632 Fragments arranged by size: 4632 2815 1123 197 AflIII A'CryG_T Cuts at: 1203 7666 8643 1203 Size: 6463 977 1327 Fragments arranged by size: 6463 1327 977 AgeI A'CCGG_T Cuts at: 7598 7598 Size: 8767 AhdI GACnn_n'nnGTC Cuts at: 2788 7496 2788 Size: 4708 4059 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8767 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 5292 1184 Size: 4108 4659 AlwNI CAG_nnn'CTG Cuts at: 6510 8082 6510 Size: 1572 7195 ApaLI G'TGCA_C Cuts at: 5214 6824 7980 5214 Size: 1610 1156 6001 Fragments arranged by size: 6001 1610 1156 ApoI r'AATT_y Cuts at: 1493 2080 2330 2887 3598 3774 3784 4469 4624 Size: 587 250 557 711 176 10 685 155 Cuts at: 4624 5144 5451 5577 6076 6302 6454 8563 1493 Size: 520 307 126 499 226 152 2109 1697 Fragments arranged by size: 2109 1697 711 685 587 557 520 499 307 250 226 176 155 152 126 10 AseI AT'TA_AT Cuts at: 160 1032 3489 7321 160 Size: 872 2457 3832 1606 Fragments arranged by size: 3832 2457 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8767 AvaI C'yCGr_G Cuts at: 1870 1943 4182 6092 1870 Size: 73 2239 1910 4545 Fragments arranged by size: 4545 2239 1910 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2691 2724 2691 Size: 33 8734 BamHI G'GATC_C Cuts at: 2062 6097 6464 2062 Size: 4035 367 4365 Fragments arranged by size: 4365 4035 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 6088 7543 618 Size: 325 217 627 4301 1455 1842 Fragments arranged by size: 4301 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 6086 1391 Size: 4695 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3582 4382 928 Size: 971 1683 800 5313 Fragments arranged by size: 5313 1683 971 800 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6958 6992 1903 Size: 34 5021 34 3678 Fragments arranged by size: 5021 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6659 7869 6659 Size: 1210 7557 BclI T'GATC_A Cuts at: 1548 1548 Size: 8767 BfrBI ATG'CAT Cuts at: 2429 2429 Size: 8767 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 7378 136 Size: 107 122 71 631 605 5706 1525 Fragments arranged by size: 5706 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2348 2482 8762 2348 Size: 134 6280 2353 Fragments arranged by size: 6280 2353 134 BlpI GC'TnA_GC Cuts at: 4482 4548 6184 4482 Size: 66 1636 7065 Fragments arranged by size: 7065 1636 66 Bme1580I G_kGCm'C Cuts at: 1790 2530 5218 6828 7984 1790 Size: 740 2688 1610 1156 2573 Fragments arranged by size: 2688 2573 1610 1156 740 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8767 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2022 3622 6498 Size: 416 53 83 399 24 605 1600 2876 Cuts at: 6498 7456 442 Size: 958 1753 Fragments arranged by size: 2876 1753 1600 958 605 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8767 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4352 4949 7427 1239 Size: 3113 597 2478 2579 Fragments arranged by size: 3113 2579 2478 597 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3954 4365 6892 7757 8055 3954 Size: 411 2527 865 298 4666 Fragments arranged by size: 4666 2527 865 411 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3873 7430 882 Size: 617 2374 3557 2219 Fragments arranged by size: 3557 2374 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 6558 493 Size: 711 5354 2702 Fragments arranged by size: 5354 2702 711 BsaBI GATnn'nnATC Cuts at: 2445 3220 4806 8761 2445 Size: 775 1586 3955 2451 Fragments arranged by size: 3955 2451 1586 775 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6574 6956 7591 275 Size: 53 83 186 936 5041 382 635 1451 Fragments arranged by size: 5041 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6030 7200 7598 7872 8019 8430 6030 Size: 1170 398 274 147 411 6367 Fragments arranged by size: 6367 1170 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2472 2502 3344 3374 3455 Size: 30 165 30 1069 30 842 30 81 Cuts at: 3455 3485 1178 Size: 30 6460 Fragments arranged by size: 6460 1069 842 165 81 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3926 4952 4955 1186 Size: 2740 1026 3 4998 Fragments arranged by size: 4998 2740 1026 3 BseYI C'CCAG_C Cuts at: 7970 8466 7970 Size: 496 8271 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3382 1163 Size: 573 1646 6548 Fragments arranged by size: 6548 1646 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6217 6978 8006 1540 Size: 275 186 4216 761 1028 2301 Fragments arranged by size: 4216 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 5218 6828 6913 7984 5218 Size: 1610 85 1071 6001 Fragments arranged by size: 6001 1610 1071 85 BsmI GAATG_Cn' Cuts at: 2429 6278 6371 2429 Size: 3849 93 4825 Fragments arranged by size: 4825 3849 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2530 5218 6086 6828 6913 7984 1391 Size: 399 740 2688 868 742 85 1071 2174 Fragments arranged by size: 2688 2174 1071 868 742 740 399 85 BspEI T'CCGG_A Cuts at: 6030 8430 6030 Size: 2400 6367 BspHI T'CATG_A Cuts at: 3859 6656 8386 3859 Size: 2797 1730 4240 Fragments arranged by size: 4240 2797 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2061 6122 844 Size: 1217 4061 3489 Fragments arranged by size: 4061 3489 1217 BsrBI CCG'CTC Cuts at: 1394 1823 6654 1394 Size: 429 4831 3507 Fragments arranged by size: 4831 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4146 7262 7436 65 Size: 1183 2898 3116 174 1396 Fragments arranged by size: 3116 2898 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 7411 7598 1700 Size: 110 5601 187 2869 Fragments arranged by size: 5601 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8767 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8767 BssSI C'ACGA_G Cuts at: 6827 7839 6827 Size: 1012 7755 BstAPI GCAn_nnn'nTGC Cuts at: 794 2564 794 Size: 1770 6997 BstBI TT'CG_AA Cuts at: 7571 7571 Size: 8767 BstEII G'GTnAC_C Cuts at: 1184 5345 1184 Size: 4161 4606 BstYI r'GATC_y Cuts at: 1972 2062 2348 2482 4681 6008 6097 6464 6849 Size: 90 286 134 2199 1327 89 367 385 Cuts at: 6849 6866 8318 8404 8416 8762 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 2199 1977 1452 1327 385 367 346 286 134 90 89 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2577 513 Size: 552 1512 6703 Fragments arranged by size: 6703 1512 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 8028 Fragments arranged by size: 8028 683 56 BtsI GCAGTG_nn' Cuts at: 6012 6283 7077 7097 6012 Size: 271 794 20 7682 Fragments arranged by size: 7682 794 271 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8767 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4116 4151 529 Size: 35 3552 35 5145 Fragments arranged by size: 5145 3552 35 35 DraI TTT'AAA Cuts at: 4023 4404 4629 5050 6071 4023 Size: 381 225 421 1021 6719 Fragments arranged by size: 6719 1021 421 381 225 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7774 809 Size: 507 6458 1802 Fragments arranged by size: 6458 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 5244 6214 7103 Size: 54 1425 50 275 18 3414 970 889 Cuts at: 7103 8732 8 Size: 1629 43 Fragments arranged by size: 3414 1629 1425 970 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6214 1537 Size: 275 4402 4090 Fragments arranged by size: 4402 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2767 3017 3405 3521 3695 3841 4116 4867 Size: 1414 250 388 116 174 146 275 751 Cuts at: 4867 4878 6697 1353 Size: 11 1819 3423 Fragments arranged by size: 3423 1819 1414 751 388 275 250 174 146 116 11 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 7354 7728 7874 387 Size: 1064 138 5765 374 146 1280 Fragments arranged by size: 5765 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2244 2754 3210 4268 4352 4949 5216 Size: 393 612 510 456 1058 84 597 267 Cuts at: 5216 5592 6830 7427 8214 8482 1239 Size: 376 1238 597 787 268 1524 Fragments arranged by size: 1524 1238 1058 787 612 597 597 510 456 393 376 268 267 84 EcoNI CCTnn'n_nnAGG Cuts at: 3186 3186 Size: 8767 EcoRI G'AATT_C Cuts at: 2080 3598 3784 6076 6454 2080 Size: 1518 186 2292 378 4393 Fragments arranged by size: 4393 2292 1518 378 186 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2570 3995 4556 4682 4981 5393 2570 Size: 1425 561 126 299 412 5944 Fragments arranged by size: 5944 1425 561 412 299 126 FspI TGC'GCA Cuts at: 7273 7273 Size: 8767 HaeII r_GCGC'y Cuts at: 1678 7914 1678 Size: 6236 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2054 2086 2774 2806 3608 Size: 32 135 32 482 32 688 32 802 Cuts at: 3608 3640 3688 3720 4115 4147 4325 4357 4936 Size: 32 48 32 395 32 178 32 579 Cuts at: 4936 4968 5385 5417 5858 5890 7408 7440 7482 Size: 32 417 32 441 32 1518 32 42 Cuts at: 7482 7514 1373 Size: 32 2626 Fragments arranged by size: 2626 1518 802 688 579 482 441 417 395 178 135 48 42 32 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2010 5014 6111 6357 669 Size: 988 281 72 3004 1097 246 3079 Fragments arranged by size: 3079 3004 1097 988 281 246 72 HindIII A'AGCT_T Cuts at: 748 2318 3159 3271 748 Size: 1570 841 112 6244 Fragments arranged by size: 6244 1570 841 112 HpaI GTT'AAC Cuts at: 5014 6357 5014 Size: 1343 7424 KpnI G_GTAC'C Cuts at: 6092 6092 Size: 8767 MfeI C'AATT_G Cuts at: 2276 5198 5270 5725 6366 2276 Size: 2922 72 455 641 4677 Fragments arranged by size: 4677 2922 641 455 72 MluI A'CGCG_T Cuts at: 8643 8643 Size: 8767 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2689 2836 3552 4659 7881 8065 1305 Size: 1384 147 716 1107 3222 184 2007 Fragments arranged by size: 3222 2007 1384 1107 716 184 147 MscI TGG'CCA Cuts at: 10 64 1489 1832 5246 10 Size: 54 1425 343 3414 3531 Fragments arranged by size: 3531 3414 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2459 2595 3713 5223 6725 7084 7243 Size: 648 1293 136 1118 1510 1502 359 159 Cuts at: 7243 518 Size: 2042 Fragments arranged by size: 2042 1510 1502 1293 1118 648 359 159 136 MspA1I CmG'CkG Cuts at: 1606 2705 3319 3686 3820 6183 6860 8008 8253 Size: 1099 614 367 134 2363 677 1148 245 Cuts at: 8253 1606 Size: 2120 Fragments arranged by size: 2363 2120 1148 1099 677 614 367 245 134 NcoI C'CATG_G Cuts at: 513 1065 2577 513 Size: 552 1512 6703 Fragments arranged by size: 6703 1512 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8767 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8767 NotI GC'GGCC_GC Cuts at: 6214 6214 Size: 8767 NsiI A_TGCA'T Cuts at: 2431 2431 Size: 8767 NspI r_CATG'y Cuts at: 1132 1229 2473 2617 3979 4846 5191 5759 5904 Size: 97 1244 144 1362 867 345 568 145 Cuts at: 5904 6125 7670 1132 Size: 221 1545 2229 Fragments arranged by size: 2229 1545 1362 1244 867 568 345 221 145 144 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8767 PciI A'CATG_T Cuts at: 7666 7666 Size: 8767 PflMI CCAn_nnn'nTGG Cuts at: 1108 2578 5969 1108 Size: 1470 3391 3906 Fragments arranged by size: 3906 3391 1470 PmeI GTTT'AAAC Cuts at: 6071 6071 Size: 8767 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6836 6868 8349 8381 6836 Size: 32 1481 32 7222 Fragments arranged by size: 7222 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3855 1613 Size: 2242 6525 PsiI TTA'TAA Cuts at: 4572 6337 4572 Size: 1765 7002 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 5986 6018 5986 Size: 32 8735 PstI C_TGCA'G Cuts at: 830 2545 6119 830 Size: 1715 3574 3478 Fragments arranged by size: 3574 3478 1715 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8767 PvuII CAG'CTG Cuts at: 3319 3686 3820 3319 Size: 367 134 8266 Fragments arranged by size: 8266 367 134 SalI G'TCGA_C Cuts at: 1936 2008 6109 1936 Size: 72 4101 4594 Fragments arranged by size: 4594 4101 72 SapI GCTCTTCn'nnn_ Cuts at: 4878 4878 Size: 8767 SbfI CC_TGCA'GG Cuts at: 6119 6119 Size: 8767 ScaI AGT'ACT Cuts at: 7015 8727 7015 Size: 1712 7055 SfcI C'TryA_G Cuts at: 826 1045 2541 3057 3441 3476 6115 7250 7931 Size: 219 1496 516 384 35 2639 1135 681 Cuts at: 7931 8122 826 Size: 191 1471 Fragments arranged by size: 2639 1496 1471 1135 681 516 384 219 191 35 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8767 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8767 SmaI CCC'GGG Cuts at: 1872 6094 1872 Size: 4222 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2140 3969 4182 4380 4955 6871 Size: 197 926 197 1829 213 198 575 1916 Cuts at: 6871 7772 8034 820 Size: 901 262 1553 Fragments arranged by size: 1916 1829 1553 926 901 575 262 213 198 197 197 SnaBI TAC'GTA Cuts at: 493 6558 493 Size: 6065 2702 SpeI A'CTAG_T Cuts at: 152 3098 6062 152 Size: 2946 2964 2857 Fragments arranged by size: 2964 2946 2857 SphI G_CATG'C Cuts at: 1132 4846 6125 1132 Size: 3714 1279 3774 Fragments arranged by size: 3774 3714 1279 SspI AAT'ATT Cuts at: 5 52 2158 4556 4866 5589 6691 5 Size: 47 2106 2398 310 723 1102 2081 Fragments arranged by size: 2398 2106 2081 1102 723 310 47 StuI AGG'CCT Cuts at: 1517 4725 1517 Size: 3208 5559 StyI C'CwwG_G Cuts at: 513 1065 2405 2577 2749 5545 6206 513 Size: 552 1340 172 172 2796 661 3074 Fragments arranged by size: 3074 2796 1340 661 552 172 172 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 6794 6811 6964 7149 7594 325 Size: 1255 5214 17 153 185 445 1498 Fragments arranged by size: 5214 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5647 7013 8725 Size: 274 80 33 51 1443 3670 1366 1712 Cuts at: 8725 96 Size: 138 Fragments arranged by size: 3670 1712 1443 1366 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3344 3376 8727 8759 3344 Size: 32 5351 32 3352 Fragments arranged by size: 5351 3352 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8767 XbaI T'CTAG_A Cuts at: 3433 6103 3433 Size: 2670 6097 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2756 5497 2756 Size: 2741 6026 XhoI C'TCGA_G Cuts at: 1943 4182 1943 Size: 2239 6528 XmaI C'CCGG_G Cuts at: 1870 6092 1870 Size: 4222 4545 XmnI GAAnn'nnTTC Cuts at: 2550 5577 5998 6896 2550 Size: 3027 421 898 4421 Fragments arranged by size: 4421 3027 898 421 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6575 276 Size: 53 83 186 936 5041 2468 Fragments arranged by size: 5041 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI