(Linear) (Six-base) MAPSORT of: KIEE3460.seq Check: 5868 from: 1 to: 4062 KIEE3460.orf With 182 enzymes: SgfI * March 22, 2012 16:34 .. AccI GT'mk_AC Cuts at: 0 4 4062 Size: 4 4058 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 239 749 1205 2263 3211 3587 4062 Size: 239 510 456 1058 948 376 475 Fragments arranged by size: 1058 948 510 475 456 376 239 AflII C'TTAA_G Cuts at: 0 135 2950 4062 Size: 135 2815 1112 Fragments arranged by size: 2815 1112 135 AhdI GACnn_n'nnGTC Cuts at: 0 783 4062 Size: 783 3279 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 3287 4062 Size: 3287 775 ApaLI G'TGCA_C Cuts at: 0 3209 4062 Size: 3209 853 ApoI r'AATT_y Cuts at: 0 75 325 882 1593 1769 1779 2464 2619 Size: 75 250 557 711 176 10 685 155 Cuts at: 2619 3139 3446 3572 4062 Size: 520 307 126 490 Fragments arranged by size: 711 685 557 520 490 307 250 176 155 126 75 10 AseI AT'TA_AT Cuts at: 0 1484 4062 Size: 1484 2578 AvaI C'yCGr_G Cuts at: 0 2177 4062 Size: 2177 1885 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 686 719 4062 Size: 686 33 3343 Fragments arranged by size: 3343 686 33 BamHI G'GATC_C Cuts at: 0 57 4062 Size: 57 4005 BbsI GAAGACnn'nnnn_ Cuts at: 0 1577 2377 4062 Size: 1577 800 1685 Fragments arranged by size: 1685 1577 800 BfrBI ATG'CAT Cuts at: 0 424 4062 Size: 424 3638 BglII A'GATC_T Cuts at: 0 343 477 4062 Size: 343 134 3585 Fragments arranged by size: 3585 343 134 BlpI GC'TnA_GC Cuts at: 0 2477 2543 4062 Size: 2477 66 1519 Fragments arranged by size: 2477 1519 66 Bme1580I G_kGCm'C Cuts at: 0 525 3213 4062 Size: 525 2688 849 Fragments arranged by size: 2688 849 525 BmrI ACTGGGnnnn_n' Cuts at: 0 17 1617 4062 Size: 17 1600 2445 Fragments arranged by size: 2445 1600 17 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2347 2944 4062 Size: 2347 597 1118 Fragments arranged by size: 2347 1118 597 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1949 2360 4062 Size: 1949 411 1702 Fragments arranged by size: 1949 1702 411 BsaI GGTCTCn'nnnn_ Cuts at: 0 1868 4062 Size: 1868 2194 BsaBI GATnn'nnATC Cuts at: 0 440 1215 2801 4062 Size: 440 775 1586 1261 Fragments arranged by size: 1586 1261 775 440 BsaWI w'CCGG_w Cuts at: 0 4025 4062 Size: 4025 37 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 467 497 1339 1369 1450 1480 4062 Size: 467 30 842 30 81 30 2582 Fragments arranged by size: 2582 842 467 81 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1921 2947 2950 4062 Size: 1921 1026 3 1112 Fragments arranged by size: 1921 1112 1026 3 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1377 4062 Size: 1377 2685 BsiHKAI G_wGCw'C Cuts at: 0 3213 4062 Size: 3213 849 BsmI GAATG_Cn' Cuts at: 0 424 4062 Size: 424 3638 Bsp1286I G_dGCh'C Cuts at: 0 525 3213 4062 Size: 525 2688 849 Fragments arranged by size: 2688 849 525 BspEI T'CCGG_A Cuts at: 0 4025 4062 Size: 4025 37 BspHI T'CATG_A Cuts at: 0 1854 4062 Size: 1854 2208 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 56 4062 Size: 56 4006 BsrDI GCAATG_nn' Cuts at: 0 2141 4062 Size: 2141 1921 BstAPI GCAn_nnn'nTGC Cuts at: 0 559 4062 Size: 559 3503 BstEII G'GTnAC_C Cuts at: 0 3340 4062 Size: 3340 722 BstYI r'GATC_y Cuts at: 0 57 343 477 2676 4003 4062 Size: 57 286 134 2199 1327 59 Fragments arranged by size: 2199 1327 286 134 59 57 BtgI C'CryG_G Cuts at: 0 572 4062 Size: 572 3490 BtsI GCAGTG_nn' Cuts at: 0 4007 4062 Size: 4007 55 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 2111 2146 4062 Size: 2111 35 1916 Fragments arranged by size: 2111 1916 35 DraI TTT'AAA Cuts at: 0 2018 2399 2624 3045 4062 Size: 2018 381 225 421 1017 Fragments arranged by size: 2018 1017 421 381 225 EaeI y'GGCC_r Cuts at: 0 3239 4062 Size: 3239 823 EarI CTCTTCn'nnn_ Cuts at: 0 762 1012 1400 1516 1690 1836 2111 2862 Size: 762 250 388 116 174 146 275 751 Cuts at: 2862 2873 4062 Size: 11 1189 Fragments arranged by size: 1189 762 751 388 275 250 174 146 116 11 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 239 749 1205 2263 2347 2944 3211 3587 Size: 239 510 456 1058 84 597 267 376 Cuts at: 3587 4062 Size: 475 Fragments arranged by size: 1058 597 510 475 456 376 267 239 84 EcoNI CCTnn'n_nnAGG Cuts at: 0 1181 4062 Size: 1181 2881 EcoRI G'AATT_C Cuts at: 0 75 1593 1779 4062 Size: 75 1518 186 2283 Fragments arranged by size: 2283 1518 186 75 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 565 1990 2551 2677 2976 3388 4062 Size: 565 1425 561 126 299 412 674 Fragments arranged by size: 1425 674 565 561 412 299 126 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 49 81 769 801 1603 1635 1683 1715 Size: 49 32 688 32 802 32 48 32 Cuts at: 1715 2110 2142 2320 2352 2931 2963 3380 3412 Size: 395 32 178 32 579 32 417 32 Cuts at: 3412 3853 3885 4062 Size: 441 32 177 Fragments arranged by size: 802 688 579 441 417 395 178 177 49 48 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 5 3009 4062 Size: 5 3004 1053 Fragments arranged by size: 3004 1053 5 HindIII A'AGCT_T Cuts at: 0 313 1154 1266 4062 Size: 313 841 112 2796 Fragments arranged by size: 2796 841 313 112 HpaI GTT'AAC Cuts at: 0 3009 4062 Size: 3009 1053 MfeI C'AATT_G Cuts at: 0 271 3193 3265 3720 4062 Size: 271 2922 72 455 342 Fragments arranged by size: 2922 455 342 271 72 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 684 831 1547 2654 4062 Size: 684 147 716 1107 1408 Fragments arranged by size: 1408 1107 716 684 147 MscI TGG'CCA Cuts at: 0 3241 4062 Size: 3241 821 MslI CAynn'nnrTG Cuts at: 0 454 590 1708 3218 4062 Size: 454 136 1118 1510 844 Fragments arranged by size: 1510 1118 844 454 136 MspA1I CmG'CkG Cuts at: 0 700 1314 1681 1815 4062 Size: 700 614 367 134 2247 Fragments arranged by size: 2247 700 614 367 134 NcoI C'CATG_G Cuts at: 0 572 4062 Size: 572 3490 NsiI A_TGCA'T Cuts at: 0 426 4062 Size: 426 3636 NspI r_CATG'y Cuts at: 0 468 612 1974 2841 3186 3754 3899 4062 Size: 468 144 1362 867 345 568 145 163 Fragments arranged by size: 1362 867 568 468 345 163 145 144 PflMI CCAn_nnn'nTGG Cuts at: 0 573 3964 4062 Size: 573 3391 98 Fragments arranged by size: 3391 573 98 PshAI GACnn'nnGTC Cuts at: 0 1850 4062 Size: 1850 2212 PsiI TTA'TAA Cuts at: 0 2567 4062 Size: 2567 1495 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 3981 4013 4062 Size: 3981 32 49 Fragments arranged by size: 3981 49 32 PstI C_TGCA'G Cuts at: 0 540 4062 Size: 540 3522 PvuII CAG'CTG Cuts at: 0 1314 1681 1815 4062 Size: 1314 367 134 2247 Fragments arranged by size: 2247 1314 367 134 SalI G'TCGA_C Cuts at: 0 3 4062 Size: 3 4059 SapI GCTCTTCn'nnn_ Cuts at: 0 2873 4062 Size: 2873 1189 SfcI C'TryA_G Cuts at: 0 536 1052 1436 1471 4062 Size: 536 516 384 35 2591 Fragments arranged by size: 2591 536 516 384 35 SmlI C'TyrA_G Cuts at: 0 135 1964 2177 2375 2950 4062 Size: 135 1829 213 198 575 1112 Fragments arranged by size: 1829 1112 575 213 198 135 SpeI A'CTAG_T Cuts at: 0 1093 4057 4062 Size: 1093 2964 5 Fragments arranged by size: 2964 1093 5 SphI G_CATG'C Cuts at: 0 2841 4062 Size: 2841 1221 SspI AAT'ATT Cuts at: 0 153 2551 2861 3584 4062 Size: 153 2398 310 723 478 Fragments arranged by size: 2398 723 478 310 153 StuI AGG'CCT Cuts at: 0 2720 4062 Size: 2720 1342 StyI C'CwwG_G Cuts at: 0 400 572 744 3540 4062 Size: 400 172 172 2796 522 Fragments arranged by size: 2796 522 400 172 172 TatI w'GTAC_w Cuts at: 0 3642 4062 Size: 3642 420 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1339 1371 4062 Size: 1339 32 2691 Fragments arranged by size: 2691 1339 32 XbaI T'CTAG_A Cuts at: 0 1428 4062 Size: 1428 2634 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 751 3492 4062 Size: 751 2741 570 Fragments arranged by size: 2741 751 570 XhoI C'TCGA_G Cuts at: 0 2177 4062 Size: 2177 1885 XmnI GAAnn'nnTTC Cuts at: 0 545 3572 3993 4062 Size: 545 3027 421 69 Fragments arranged by size: 3027 545 421 69 Enzymes that do cut: AccI AcuI AflII AhdI AlfI ApaLI ApoI AseI AvaI BaeI BamHI BbsI BfrBI BglII BlpI Bme1580I BmrI BpmI BpuEI BsaI BsaBI BsaWI BsaXI BseRI BsgI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrDI BstAPI BstEII BstYI BtgI BtsI CspCI DraI EaeI EarI Eco57MI EcoNI EcoRI FalI Hin4I HincII HindIII HpaI MfeI MmeI MscI MslI MspA1I NcoI NsiI NspI PflMI PshAI PsiI PsrI PstI PvuII SalI SapI SfcI SmlI SpeI SphI SspI StuI StyI TatI TstI XbaI XcmI XhoI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflIII AgeI AleI AloI AlwNI ApaI AscI AsiSI AvrII BanI BanII BbeI BbvCI BcgI BciVI BclI BglI BmgBI BmtI BplI Bpu10I BsaAI BsaHI BseYI BsiEI BsiWI BsmBI BsrBI BsrFI BsrGI BssHII BssSI BstBI BstXI BstZ17I Bsu36I BtgZI ClaI DraIII DrdI EagI EciI EcoICRI EcoO109I EcoRV FseI FspI FspAI HaeII KasI KpnI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI PacI PasI PciI PfoI PmeI PmlI PpiI PpuMI PspOMI PspXI PvuI RsrII SacI SacII SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SrfI SwaI TaqII Tth111I XmaI ZraI