################################## # NO CUT ENZYMES ( 128 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwI AocI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BsaBI BsaI BsaWI BseAI BseRI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspLU11I BsrDI BsrFI BsrGI Bst1107I BstBI BstYI BstZ17I Bsu36I Cfr10I ClaI CpoI DpnII DraI DraIII Eam1105I Eco81I EcoNI EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII HpaI KpnI MaeI MamI MboI MfeI MflI MluI MroI MseI MunI NaeI NdeI NdeII NgoMIV NheI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacI SalI Sau3AI SbfI ScaI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tru9I Tsp509I Tth111I TthHB8I XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AccI 262 GTCTAC GT^[AC][GT]AC AcyI 864 GGCGCC G[GA]^CG[CT]C AlwNI 99 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG AspHI 373 GTGCTC G[AT]GC[AT]^C BbeI 864 GGCGCC GGCGC^C BbsI 1077 GTCTTC GAAGAC(2/6) BlpI 303 GCTCAGC GC^T[ACGT]AGC BpmI 133 CTGGAG CTGGAG(16/14) Bpu1102I 303 GCTCAGC GC^T[ACGT]AGC BpuAI 1077 GTCTTC GAAGAC(2/6) BsaAI 265 TACGTG [CT]AC^GT[GA] BsaHI 864 GGCGCC G[GA]^CG[CT]C BsgI 475 GTGCAG GTGCAG(16/14) BsiHKAI 373 GTGCTC G[AT]GC[AT]^C BspHI 428 TCATGA T^CATGA BssSI 1108 CTCGTG CACGAG(-5/-1) BstAPI 917 GCAACAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 222 GGTCACC G^GT[ACGT]ACC BstF5I 309 CATCC GGATG(2/0) BstPI 222 GGTCACC G^GT[ACGT]ACC CelII 303 GCTCAGC GC^T[ACGT]AGC DdeI 304 CTCAG C^T[ACGT]AG EcoO65I 222 GGTCACC G^GT[ACGT]ACC FokI 309 CATCC GGATG(9/13) Hin1I 864 GGCGCC G[GA]^CG[CT]C KasI 864 GGCGCC G^GCGCC KspI 868 CCGCGG CCGC^GG NarI 864 GGCGCC GG^CGCC NcoI 179 CCATGG C^CATGG PflMI 338 CCAAGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RcaI 428 TCATGA T^CATGA SacII 868 CCGCGG CCGC^GG SexAI 140 ACCTGGT A^CC[AT]GGT SfiI 1116 GGCCCCAGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 864 GGCGCC GGC^GCC Van91I 338 CCAAGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 179 CCATGGGCGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### Aor51HI 440 AGCGCT AGC^GCT 1055 AGCGCT BsrI 49 ACTGG ACTGG(1/-1) 843 CCAGT DrdI 9 GACCCCCGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 165 GACTCTGCTGTC Eco47III 440 AGCGCT AGC^GCT 1055 AGCGCT HgaI 540 GACGC GACGC(5/10) 636 GACGC HincII 271 GTCAAC GT[CT]^[GA]AC 886 GTCAAC HindII 271 GTCAAC GT[CT]^[GA]AC 886 GTCAAC HinfI 16 GAGTC G^A[ACGT]TC 165 GACTC MaeIII 223 GTCAC ^GT[ACGT]AC 334 GTCAC MslI 180 CATGGGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 213 CACGCTGGTG NotI 459 GCGGCCGC GC^GGCCGC 980 GCGGCCGC PleI 165 GACTC GAGTC(4/5) 16 GAGTC PstI 1033 CTGCAG CTGCA^G 1083 CTGCAG PvuII 832 CAGCTG CAG^CTG 921 CAGCTG SfcI 1033 CTGCAG C^T[GA][CT]AG 1083 CTGCAG SmaI 667 CCCGGG CCC^GGG 996 CCCGGG Tsp45I 223 GTCAC ^GT[GC]AC 334 GTCAC TspRI 48 CACTG CA[GC]TG[ACGT][ACGT]^ 1098 CACTG XmaCI 667 CCCGGG C^CCGGG 996 CCCGGG XmaI 667 CCCGGG C^CCGGG 996 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 73 enzymes) # ######################################### AccII AciI AfaI AluI ApaI AvaI AvaII BalI BanI BanII BbvI BcnI BglI BmyI BsaJI BsiEI BsiYI BslI BsmFI BsoBI Bsp1286I BspMI BsrBI BssHII BssKI BstNI BstUI BstXI Cac8I CfoI Cfr13I DraII DsaI EaeI EagI EarI EclXI Eco52I EcoO109I EcoRII EcoT14I FauI Fnu4HI HaeII HaeIII HapII HhaI HinP1I HpaII HphI ItaI Ksp632I MaeII MboII MluNI MnlI MscI MspA1I MspI MvaI MvnI MwoI NciI NlaIII NlaIV PpuMI RsaI SapI Sau96I ScrFI SfaNI StyI TseI