(Circular) (Six-base) MAPSORT of: pFN21AE0842.seq Check: 9288 from: 1 to: 5872 pFN21AE0842 5872 bp With 182 enzymes: SgfI * December 28, 2011 15:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2240 2915 2988 2240 Size: 675 73 5124 Fragments arranged by size: 5124 675 73 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3682 278 Size: 53 83 186 936 2146 2468 Fragments arranged by size: 2468 2146 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2268 3215 1937 Size: 331 947 4594 Fragments arranged by size: 4594 947 331 Acc65I G'GTAC_C Cuts at: 3193 3193 Size: 5872 AclI AA'CG_TT Cuts at: 1563 3999 4372 1563 Size: 2436 373 3063 Fragments arranged by size: 3063 2436 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3935 5319 5587 1632 Size: 2303 1384 268 1917 Fragments arranged by size: 2303 1917 1384 268 AfeI AGC'GCT Cuts at: 2447 3062 2447 Size: 615 5257 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5675 AflIII A'CryG_T Cuts at: 1203 4771 5748 1203 Size: 3568 977 1327 Fragments arranged by size: 3568 1327 977 AgeI A'CCGG_T Cuts at: 4703 4703 Size: 5872 AhdI GACnn_n'nnGTC Cuts at: 4601 4601 Size: 5872 AleI CACnn'nnGTG Cuts at: 1166 2222 1166 Size: 1056 4816 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2081 2543 2813 1184 Size: 897 462 270 4243 Fragments arranged by size: 4243 897 462 270 AlwNI CAG_nnn'CTG Cuts at: 2109 3615 5187 2109 Size: 1506 1572 2794 Fragments arranged by size: 2794 1572 1506 ApaI G_GGCC'C Cuts at: 2618 2673 2752 3002 3027 3166 2618 Size: 55 79 250 25 139 5324 Fragments arranged by size: 5324 250 139 79 55 25 ApaLI G'TGCA_C Cuts at: 3929 5085 3929 Size: 1156 4716 ApoI r'AATT_y Cuts at: 1493 3181 3407 3559 5668 1493 Size: 1688 226 152 2109 1697 Fragments arranged by size: 2109 1697 1688 226 152 AseI AT'TA_AT Cuts at: 160 1032 4426 160 Size: 872 3394 1606 Fragments arranged by size: 3394 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5872 AvaI C'yCGr_G Cuts at: 1870 1943 2018 2026 2672 3001 3149 3197 1870 Size: 73 75 8 646 329 148 48 4545 Fragments arranged by size: 4545 646 329 148 75 73 48 8 BamHI G'GATC_C Cuts at: 3202 3569 3202 Size: 367 5505 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2159 2519 2869 3193 4648 Size: 325 217 627 372 360 350 324 1455 Cuts at: 4648 618 Size: 1842 Fragments arranged by size: 1842 1455 627 372 360 350 325 324 217 BanII G_rGCy'C Cuts at: 1391 2130 2618 2673 2752 3002 3027 3166 3191 Size: 739 488 55 79 250 25 139 25 Cuts at: 3191 1391 Size: 4072 Fragments arranged by size: 4072 739 488 250 139 79 55 25 25 BbeI G_GCGC'C Cuts at: 2873 2873 Size: 5872 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3075 928 Size: 971 1176 3725 Fragments arranged by size: 3725 1176 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4063 4097 1903 Size: 34 2126 34 3678 Fragments arranged by size: 3678 2126 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3764 4974 3764 Size: 1210 4662 BclI T'GATC_A Cuts at: 1548 1548 Size: 5872 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2493 2827 2867 Size: 107 122 71 631 605 821 334 40 Cuts at: 2867 3128 4483 136 Size: 261 1355 1525 Fragments arranged by size: 1525 1355 821 631 605 334 261 122 107 71 40 BglII A'GATC_T Cuts at: 5867 5867 Size: 5872 BlpI GC'TnA_GC Cuts at: 2309 3289 2309 Size: 980 4892 Bme1580I G_kGCm'C Cuts at: 1790 2162 2522 2618 2673 2752 3002 3027 3166 Size: 372 360 96 55 79 250 25 139 Cuts at: 3166 3933 5089 1790 Size: 767 1156 2573 Fragments arranged by size: 2573 1156 767 372 360 250 139 96 79 55 25 BmgBI CAC'GTC Cuts at: 1318 2365 1318 Size: 1047 4825 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2842 3603 4561 Size: 416 53 83 399 24 1425 761 958 Cuts at: 4561 442 Size: 1753 Fragments arranged by size: 1753 1425 958 761 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5872 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2149 2149 Size: 5872 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2159 4532 1239 Size: 920 2373 2579 Fragments arranged by size: 2579 2373 920 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3997 4862 5160 3997 Size: 865 298 4709 Fragments arranged by size: 4709 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4535 882 Size: 617 3036 2219 Fragments arranged by size: 3036 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2272 3663 493 Size: 711 1068 1391 2702 Fragments arranged by size: 2702 1391 1068 711 BsaBI GATnn'nnATC Cuts at: 5866 5866 Size: 5872 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2870 3679 4061 4696 Size: 53 83 186 936 1337 809 382 635 Cuts at: 4696 275 Size: 1451 Fragments arranged by size: 1451 1337 936 809 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4305 4703 4977 5124 5535 4305 Size: 398 274 147 411 4642 Fragments arranged by size: 4642 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5647 Fragments arranged by size: 5647 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5872 BseYI C'CCAG_C Cuts at: 2129 2515 2785 3124 5075 5571 2129 Size: 386 270 339 1951 496 2430 Fragments arranged by size: 2430 1951 496 386 339 270 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2501 1163 Size: 573 765 4534 Fragments arranged by size: 4534 765 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2124 2468 2759 2989 3322 4083 Size: 275 186 123 344 291 230 333 761 Cuts at: 4083 5111 1540 Size: 1028 2301 Fragments arranged by size: 2301 1028 761 344 333 291 275 230 186 123 BsiHKAI G_wGCw'C Cuts at: 2382 3933 4018 5089 2382 Size: 1551 85 1071 3165 Fragments arranged by size: 3165 1551 1071 85 BsmI GAATG_Cn' Cuts at: 3383 3476 3383 Size: 93 5779 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2130 2162 2382 2522 2618 2673 2752 Size: 399 340 32 220 140 96 55 79 Cuts at: 2752 3002 3027 3166 3191 3933 4018 5089 1391 Size: 250 25 139 25 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 340 250 220 140 139 96 85 79 55 32 25 25 BspEI T'CCGG_A Cuts at: 5535 5535 Size: 5872 BspHI T'CATG_A Cuts at: 2433 3761 5491 2433 Size: 1328 1730 2814 Fragments arranged by size: 2814 1730 1328 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2240 2304 2313 2915 2988 3151 3227 844 Size: 1396 64 9 602 73 163 76 3489 Fragments arranged by size: 3489 1396 602 163 76 73 64 9 BsrBI CCG'CTC Cuts at: 1394 1823 2238 2724 3075 3759 1394 Size: 429 415 486 351 684 3507 Fragments arranged by size: 3507 684 486 429 415 351 BsrDI GCAATG_nn' Cuts at: 65 1248 4367 4541 65 Size: 1183 3119 174 1396 Fragments arranged by size: 3119 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4516 4703 1700 Size: 110 2706 187 2869 Fragments arranged by size: 2869 2706 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5872 BssHII G'CGCG_C Cuts at: 1925 2620 2699 2731 2765 2767 2769 2882 1925 Size: 695 79 32 34 2 2 113 4915 Fragments arranged by size: 4915 695 113 79 34 32 2 2 BssSI C'ACGA_G Cuts at: 3113 3932 4944 3113 Size: 819 1012 4041 Fragments arranged by size: 4041 1012 819 BstAPI GCAn_nnn'nTGC Cuts at: 794 2928 794 Size: 2134 3738 BstBI TT'CG_AA Cuts at: 4676 4676 Size: 5872 BstEII G'GTnAC_C Cuts at: 1184 2227 1184 Size: 1043 4829 BstXI CCAn_nnnn'nTGG Cuts at: 2137 2191 2572 2137 Size: 54 381 5437 Fragments arranged by size: 5437 381 54 BstYI r'GATC_y Cuts at: 1972 3202 3569 3954 3971 5423 5509 5521 5867 Size: 1230 367 385 17 1452 86 12 346 Cuts at: 5867 1972 Size: 1977 Fragments arranged by size: 1977 1452 1230 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2154 2184 2873 513 Size: 552 939 150 30 689 3512 Fragments arranged by size: 3512 939 689 552 150 30 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2647 487 Size: 683 56 1421 3712 Fragments arranged by size: 3712 1421 683 56 BtsI GCAGTG_nn' Cuts at: 3388 4182 4202 3388 Size: 794 20 5058 Fragments arranged by size: 5058 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5872 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5837 DraI TTT'AAA Cuts at: 3176 3176 Size: 5872 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2020 2176 4879 809 Size: 507 704 156 2703 1802 Fragments arranged by size: 2703 1802 704 507 156 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2046 2151 2181 Size: 54 1425 50 275 18 216 105 30 Cuts at: 2181 2289 2328 2465 2562 2756 2986 3319 4208 Size: 108 39 137 97 194 230 333 889 Cuts at: 4208 5837 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 333 275 230 216 194 137 108 105 97 54 50 43 39 30 18 EagI C'GGCC_G Cuts at: 1537 1812 2465 2756 2986 3319 1537 Size: 275 653 291 230 333 4090 Fragments arranged by size: 4090 653 333 291 275 230 EarI CTCTTCn'nnn_ Cuts at: 1353 2387 2654 2807 3802 1353 Size: 1034 267 153 995 3423 Fragments arranged by size: 3423 1034 995 267 153 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4459 4833 4979 387 Size: 1064 138 2870 374 146 1280 Fragments arranged by size: 2870 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2159 3935 4532 5319 5587 1239 Size: 393 527 1776 597 787 268 1524 Fragments arranged by size: 1776 1524 787 597 527 393 268 EcoO109I rG'GnC_Cy Cuts at: 2143 2585 2614 2669 2860 2998 3023 3155 3162 Size: 442 29 55 191 138 25 132 7 Cuts at: 3162 3163 2143 Size: 1 4852 Fragments arranged by size: 4852 442 191 138 132 55 29 25 7 1 EcoRI G'AATT_C Cuts at: 3181 3559 3181 Size: 378 5494 FspI TGC'GCA Cuts at: 4378 4378 Size: 5872 HaeII r_GCGC'y Cuts at: 1678 2012 2288 2449 2488 2516 2537 2873 3064 Size: 334 276 161 39 28 21 336 191 Cuts at: 3064 5019 1678 Size: 1955 2531 Fragments arranged by size: 2531 1955 336 334 276 191 161 39 28 21 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4513 4545 4587 4619 1373 Size: 32 135 32 2941 32 42 32 2626 Fragments arranged by size: 2941 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2278 2893 3216 3462 669 Size: 988 281 340 615 323 246 3079 Fragments arranged by size: 3079 988 615 340 323 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5872 HpaI GTT'AAC Cuts at: 3462 3462 Size: 5872 KasI G'GCGC_C Cuts at: 2869 2869 Size: 5872 KpnI G_GTAC'C Cuts at: 3197 3197 Size: 5872 MfeI C'AATT_G Cuts at: 3471 3471 Size: 5872 MluI A'CGCG_T Cuts at: 5748 5748 Size: 5872 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4986 5170 1305 Size: 3681 184 2007 Fragments arranged by size: 3681 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2153 2330 2564 10 Size: 54 1425 343 321 177 234 3318 Fragments arranged by size: 3318 1425 343 321 234 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 2189 2222 3830 4189 4348 518 Size: 648 1023 33 1608 359 159 2042 Fragments arranged by size: 2042 1608 1023 648 359 159 33 MspA1I CmG'CkG Cuts at: 1606 2470 2839 2866 2875 2928 3009 3128 3288 Size: 864 369 27 9 53 81 119 160 Cuts at: 3288 3965 5113 5358 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 864 677 369 245 160 119 81 53 27 9 NarI GG'CG_CC Cuts at: 2870 2870 Size: 5872 NcoI C'CATG_G Cuts at: 513 1065 2004 2184 513 Size: 552 939 180 4201 Fragments arranged by size: 4201 939 552 180 NdeI CA'TA_TG Cuts at: 387 387 Size: 5872 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5872 NotI GC'GGCC_GC Cuts at: 2465 2986 3319 2465 Size: 521 333 5018 Fragments arranged by size: 5018 521 333 NspI r_CATG'y Cuts at: 1132 1229 3230 4775 1132 Size: 97 2001 1545 2229 Fragments arranged by size: 2229 2001 1545 97 PasI CC'CwG_GG Cuts at: 1348 2039 3159 1348 Size: 691 1120 4061 Fragments arranged by size: 4061 1120 691 PciI A'CATG_T Cuts at: 4771 4771 Size: 5872 PflMI CCAn_nnn'nTGG Cuts at: 1108 2349 1108 Size: 1241 4631 PmeI GTTT'AAAC Cuts at: 3176 3176 Size: 5872 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3941 3973 5454 5486 3941 Size: 32 1481 32 4327 Fragments arranged by size: 4327 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2143 2585 3155 2143 Size: 442 570 4860 Fragments arranged by size: 4860 570 442 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5872 PsiI TTA'TAA Cuts at: 3442 3442 Size: 5872 PspOMI G'GGCC_C Cuts at: 2614 2669 2748 2998 3023 3162 2614 Size: 55 79 250 25 139 5324 Fragments arranged by size: 5324 250 139 79 55 25 PstI C_TGCA'G Cuts at: 830 3042 3092 3224 830 Size: 2212 50 132 3478 Fragments arranged by size: 3478 2212 132 50 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5872 PvuII CAG'CTG Cuts at: 2839 2928 2839 Size: 89 5783 SacII CC_GC'GG Cuts at: 2876 2876 Size: 5872 SalI G'TCGA_C Cuts at: 1936 3214 1936 Size: 1278 4594 SanDI GG'GwC_CC Cuts at: 2585 3155 2585 Size: 570 5302 SapI GCTCTTCn'nnn_ Cuts at: 2387 2654 2807 2387 Size: 267 153 5452 Fragments arranged by size: 5452 267 153 SbfI CC_TGCA'GG Cuts at: 3224 3224 Size: 5872 ScaI AGT'ACT Cuts at: 4120 5832 4120 Size: 1712 4160 SexAI A'CCwGG_T Cuts at: 2145 2145 Size: 5872 SfcI C'TryA_G Cuts at: 826 1045 3038 3088 3220 4355 5036 5227 826 Size: 219 1993 50 132 1135 681 191 1471 Fragments arranged by size: 1993 1471 1135 681 219 191 132 50 SfiI GGCCn_nnn'nGGCC Cuts at: 3128 3128 Size: 5872 SfoI GGC'GCC Cuts at: 2871 2871 Size: 5872 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5872 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5872 SmaI CCC'GGG Cuts at: 1872 2674 3003 3199 1872 Size: 802 329 196 4545 Fragments arranged by size: 4545 802 329 196 SmlI C'TyrA_G Cuts at: 820 1017 1943 3976 4877 5139 820 Size: 197 926 2033 901 262 1553 Fragments arranged by size: 2033 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3663 493 Size: 3170 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5872 SphI G_CATG'C Cuts at: 1132 3230 1132 Size: 2098 3774 SrfI GCCC'GGGC Cuts at: 2674 3003 2674 Size: 329 5543 SspI AAT'ATT Cuts at: 5 52 3796 5 Size: 47 3744 2081 Fragments arranged by size: 3744 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5872 StyI C'CwwG_G Cuts at: 513 1065 2004 2184 2343 2490 3311 513 Size: 552 939 180 159 147 821 3074 Fragments arranged by size: 3074 939 821 552 180 159 147 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2138 3899 3916 4069 4254 4699 325 Size: 1255 558 1761 17 153 185 445 1498 Fragments arranged by size: 1761 1498 1255 558 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4118 5830 96 Size: 274 80 33 51 1443 2141 1712 138 Fragments arranged by size: 2141 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5832 5864 5832 Size: 32 5840 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5872 XbaI T'CTAG_A Cuts at: 3208 3208 Size: 5872 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2191 2191 Size: 5872 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5872 XmaI C'CCGG_G Cuts at: 1870 2672 3001 3197 1870 Size: 802 329 196 4545 Fragments arranged by size: 4545 802 329 196 XmnI GAAnn'nnTTC Cuts at: 4001 4001 Size: 5872 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3680 276 Size: 53 83 186 936 2146 2468 Fragments arranged by size: 2468 2146 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SwaI