################################## # NO CUT ENZYMES ( 143 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglI BlnI BpmI BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiWI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstXI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoRV EcoT22I FbaI FseI FspI HaeII Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MaeI MaeII MamI MfeI MluI MroI MspA1I MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacII SalI SapI SbfI SexAI SfcI SfiI SfoI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AcsI 286 GAATTC [GA]^AATT[CT] AluI 271 AGCT AG^CT AlwI 518 GGATC GGATC(4/5) AlwNI 363 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 208 GGGCCC GGGCC^C ApoI 286 GAATTC [GA]^AATT[CT] AspI 508 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 65 TGGCCA TGG^CCA BglII 278 AGATCT A^GATCT BseRI 571 CTCCTC GAGGAG(10/8) BsmBI 456 CGTCTC CGTCTC(1/5) BsmI 617 GAATGC GAATGC(1/-1) BsrI 92 ACTGG ACTGG(1/-1) BstAPI 197 GCAAGACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 283 TTCGAA TT^CGAA BstEII 81 GGTAACC G^GT[ACGT]ACC BstPI 81 GGTAACC G^GT[ACGT]ACC BstYI 278 AGATCT [GA]^GATC[CT] EaeI 65 TGGCCA [CT]^GGCC[GA] EcoNI 242 CCTACTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 81 GGTAACC G^GT[ACGT]ACC EcoRI 286 GAATTC G^AATTC HgaI 418 GCGTC GACGC(5/10) HinfI 637 GAGTC G^A[ACGT]TC MflI 278 AGATCT [GA]^GATC[CT] MluNI 65 TGGCCA TGG^CCA MscI 65 TGGCCA TGG^CCA MseI 257 TTAA T^TAA MslI 180 CATCCCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NspV 283 TTCGAA TT^CGAA PflFI 508 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 637 GAGTC GAGTC(4/5) PshAI 413 GACCCGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 270 GAGCTC GAGCT^C ScaI 524 AGTACT AGT^ACT SfuI 283 TTCGAA TT^CGAA SmaI 223 CCCGGG CCC^GGG Tru9I 257 TTAA T^TAA Tsp45I 31 GTGAC ^GT[GC]AC Tsp509I 287 AATT ^AATT Tth111I 508 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 68 CCATGACAGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 278 AGATCT [GA]^GATC[CT] XmaCI 223 CCCGGG C^CCGGG XmaI 223 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AccII 28 CGCG CG^CG 417 CGCG AvaII 24 GGTCC G^G[AT]CC 539 GGTCC BlpI 102 GCTGAGC GC^T[ACGT]AGC 652 GCTGAGC Bpu1102I 102 GCTGAGC GC^T[ACGT]AGC 652 GCTGAGC BsaI 442 GGTCTC GGTCTC(1/5) 586 GGTCTC BstUI 28 CGCG CG^CG 417 CGCG CelII 102 GCTGAGC GC^T[ACGT]AGC 652 GCTGAGC DpnII 279 GATC ^GATC 519 GATC EarI 37 CTCTTC CTCTTC(1/4) 393 GAAGAG FauI 415 CCCGC CCCGC(4/6) 206 GCGGG Ksp632I 37 CTCTTC CTCTTC(1/4) 393 GAAGAG MaeIII 31 GTGAC ^GT[ACGT]AC 82 GTAAC MboI 279 GATC ^GATC 519 GATC MvnI 28 CGCG CG^CG 417 CGCG NcoI 185 CCATGG C^CATGG 631 CCATGG NdeII 279 GATC ^GATC 519 GATC NlaIV 208 GGGCCC GG[ACGT]^[ACGT]CC 626 GGAACC Sau3AI 279 GATC ^GATC 519 GATC TspRI 91 CACTG CA[GC]TG[ACGT][ACGT]^ 501 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 51 enzymes) # ######################################### AciI AfaI AspHI AvaI BanII BbvI BcnI BmyI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DsaI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII HphI ItaI MboII MnlI MspI MvaI MwoI NciI NlaIII RsaI Sau96I ScrFI SfaNI StyI TaqI TseI TthHB8I