(Circular) (Six-base) MAPSORT of: pFN21AB9099.seq Check: 5178 from: 1 to: 5371 pFN21AB9099 5371 bp With 182 enzymes: SgfI * May 29, 2012 13:19 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3181 278 Size: 53 83 186 936 1645 2468 Fragments arranged by size: 2468 1645 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2714 1937 Size: 777 4594 Acc65I G'GTAC_C Cuts at: 2692 2692 Size: 5371 AclI AA'CG_TT Cuts at: 1563 3498 3871 1563 Size: 1935 373 3063 Fragments arranged by size: 3063 1935 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2045 2236 2326 3434 4818 5086 1632 Size: 413 191 90 1108 1384 268 1917 Fragments arranged by size: 1917 1384 1108 413 268 191 90 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5174 AflIII A'CryG_T Cuts at: 1203 4270 5247 1203 Size: 3067 977 1327 Fragments arranged by size: 3067 1327 977 AgeI A'CCGG_T Cuts at: 4202 4202 Size: 5371 AhdI GACnn_n'nnGTC Cuts at: 4100 4100 Size: 5371 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5371 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2467 1184 Size: 1283 4088 AlwNI CAG_nnn'CTG Cuts at: 2373 3114 4686 2373 Size: 741 1572 3058 Fragments arranged by size: 3058 1572 741 ApaI G_GGCC'C Cuts at: 2217 2217 Size: 5371 ApaLI G'TGCA_C Cuts at: 3428 4584 3428 Size: 1156 4215 ApoI r'AATT_y Cuts at: 1493 2291 2680 2906 3058 5167 1493 Size: 798 389 226 152 2109 1697 Fragments arranged by size: 2109 1697 798 389 226 152 AseI AT'TA_AT Cuts at: 160 1032 3925 160 Size: 872 2893 1606 Fragments arranged by size: 2893 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5371 AvaI C'yCGr_G Cuts at: 1870 1943 2228 2390 2663 2696 1870 Size: 73 285 162 273 33 4545 Fragments arranged by size: 4545 285 273 162 73 33 BamHI G'GATC_C Cuts at: 2701 3068 2701 Size: 367 5004 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2692 4147 618 Size: 325 217 627 905 1455 1842 Fragments arranged by size: 1842 1455 905 627 325 217 BanII G_rGCy'C Cuts at: 1391 2114 2217 2279 2664 2690 1391 Size: 723 103 62 385 26 4072 Fragments arranged by size: 4072 723 385 103 62 26 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4400 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3562 3596 1903 Size: 34 1625 34 3678 Fragments arranged by size: 3678 1625 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3263 4473 3263 Size: 1210 4161 BclI T'GATC_A Cuts at: 1548 1548 Size: 5371 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3982 136 Size: 107 122 71 631 605 2310 1525 Fragments arranged by size: 2310 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2283 5366 2283 Size: 3083 2288 BlpI GC'TnA_GC Cuts at: 2108 2658 2788 2108 Size: 550 130 4691 Fragments arranged by size: 4691 550 130 Bme1580I G_kGCm'C Cuts at: 1790 2217 3432 4588 1790 Size: 427 1215 1156 2573 Fragments arranged by size: 2573 1215 1156 427 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5371 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2107 3102 4060 Size: 416 53 83 399 24 690 995 958 Cuts at: 4060 442 Size: 1753 Fragments arranged by size: 1753 995 958 690 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5371 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4031 1239 Size: 2792 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3496 4361 4659 3496 Size: 865 298 4208 Fragments arranged by size: 4208 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2453 2597 4034 882 Size: 617 954 144 1437 2219 Fragments arranged by size: 2219 1437 954 617 144 BsaAI yAC'GTr Cuts at: 493 1204 3162 493 Size: 711 1958 2702 Fragments arranged by size: 2702 1958 711 BsaBI GATnn'nnATC Cuts at: 5365 5365 Size: 5371 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3178 3560 4195 275 Size: 53 83 186 936 1645 382 635 1451 Fragments arranged by size: 1645 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3804 4202 4476 4623 5034 3804 Size: 398 274 147 411 4141 Fragments arranged by size: 4141 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2522 2552 1178 Size: 30 165 30 1119 30 3997 Fragments arranged by size: 3997 1119 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2567 1186 Size: 1381 3990 BseYI C'CCAG_C Cuts at: 4574 5070 4574 Size: 496 4875 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4798 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2821 3582 4610 1540 Size: 275 186 820 761 1028 2301 Fragments arranged by size: 2301 1028 820 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2127 2279 2670 3432 3517 4588 2127 Size: 152 391 762 85 1071 2910 Fragments arranged by size: 2910 1071 762 391 152 85 BsmI GAATG_Cn' Cuts at: 2628 2882 2975 2628 Size: 254 93 5024 Fragments arranged by size: 5024 254 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2467 2467 Size: 5371 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2114 2127 2217 2279 2664 2670 2690 Size: 399 324 13 90 62 385 6 20 Cuts at: 2690 3432 3517 4588 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 385 324 90 85 62 20 13 6 BspEI T'CCGG_A Cuts at: 5034 5034 Size: 5371 BspHI T'CATG_A Cuts at: 3260 4990 3260 Size: 1730 3641 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2147 2216 2435 2726 844 Size: 1303 69 219 291 3489 Fragments arranged by size: 3489 1303 291 219 69 BsrBI CCG'CTC Cuts at: 1394 1823 3258 1394 Size: 429 1435 3507 Fragments arranged by size: 3507 1435 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3866 4040 65 Size: 1183 2618 174 1396 Fragments arranged by size: 2618 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4015 4202 1700 Size: 110 2205 187 2869 Fragments arranged by size: 2869 2205 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5371 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5371 BssSI C'ACGA_G Cuts at: 3431 4443 3431 Size: 1012 4359 BstAPI GCAn_nnn'nTGC Cuts at: 794 2208 794 Size: 1414 3957 BstBI TT'CG_AA Cuts at: 2289 4175 2289 Size: 1886 3485 BstEII G'GTnAC_C Cuts at: 1184 2086 1184 Size: 902 4469 BstYI r'GATC_y Cuts at: 1972 2283 2701 3068 3453 3470 4922 5008 5020 Size: 311 418 367 385 17 1452 86 12 Cuts at: 5020 5366 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 418 385 367 346 311 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2082 2190 2636 513 Size: 552 1017 108 446 3248 Fragments arranged by size: 3248 1017 552 446 108 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2317 487 Size: 683 56 1091 3541 Fragments arranged by size: 3541 1091 683 56 BtsI GCAGTG_nn' Cuts at: 2887 3681 3701 2887 Size: 794 20 4557 Fragments arranged by size: 4557 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5371 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5336 DraI TTT'AAA Cuts at: 2675 2675 Size: 5371 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4378 809 Size: 507 3062 1802 Fragments arranged by size: 3062 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2070 2818 3707 Size: 54 1425 50 275 18 240 748 889 Cuts at: 3707 5336 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 748 275 240 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2818 1537 Size: 275 1006 4090 Fragments arranged by size: 4090 1006 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2048 2393 3301 1353 Size: 695 345 908 3423 Fragments arranged by size: 3423 908 695 345 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2182 3958 4332 4478 387 Size: 1064 138 593 1776 374 146 1280 Fragments arranged by size: 1776 1280 1064 593 374 146 138 EcoICRI GAG'CTC Cuts at: 2277 2277 Size: 5371 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2045 2236 2326 3434 4031 4818 5086 Size: 393 413 191 90 1108 597 787 268 Cuts at: 5086 1239 Size: 1524 Fragments arranged by size: 1524 1108 787 597 413 393 268 191 90 EcoNI CCTnn'n_nnAGG Cuts at: 2251 2251 Size: 5371 EcoRI G'AATT_C Cuts at: 2291 2680 3058 2291 Size: 389 378 4604 Fragments arranged by size: 4604 389 378 FspI TGC'GCA Cuts at: 3877 3877 Size: 5371 HaeII r_GCGC'y Cuts at: 1678 4518 1678 Size: 2840 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2392 2424 4012 4044 4086 Size: 32 135 32 820 32 1588 32 42 Cuts at: 4086 4118 1373 Size: 32 2626 Fragments arranged by size: 2626 1588 820 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2715 2961 669 Size: 988 281 777 246 3079 Fragments arranged by size: 3079 988 777 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5371 HpaI GTT'AAC Cuts at: 2961 2961 Size: 5371 KpnI G_GTAC'C Cuts at: 2696 2696 Size: 5371 MfeI C'AATT_G Cuts at: 2970 2970 Size: 5371 MluI A'CGCG_T Cuts at: 5247 5247 Size: 5371 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4485 4669 1305 Size: 3180 184 2007 Fragments arranged by size: 3180 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2072 10 Size: 54 1425 343 240 3309 Fragments arranged by size: 3309 1425 343 240 54 MslI CAynn'nnrTG Cuts at: 518 1166 2189 3329 3688 3847 518 Size: 648 1023 1140 359 159 2042 Fragments arranged by size: 2042 1140 1023 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2787 3464 4612 4857 1606 Size: 1181 677 1148 245 2120 Fragments arranged by size: 2120 1181 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2190 2636 513 Size: 552 1125 446 3248 Fragments arranged by size: 3248 1125 552 446 NdeI CA'TA_TG Cuts at: 387 387 Size: 5371 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5371 NotI GC'GGCC_GC Cuts at: 2818 2818 Size: 5371 NspI r_CATG'y Cuts at: 1132 1229 2729 4274 1132 Size: 97 1500 1545 2229 Fragments arranged by size: 2229 1545 1500 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5371 PciI A'CATG_T Cuts at: 4270 4270 Size: 5371 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5371 PmeI GTTT'AAAC Cuts at: 2675 2675 Size: 5371 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3440 3472 4953 4985 3440 Size: 32 1481 32 3826 Fragments arranged by size: 3826 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2422 1613 Size: 809 4562 PsiI TTA'TAA Cuts at: 2941 2941 Size: 5371 PspOMI G'GGCC_C Cuts at: 2213 2213 Size: 5371 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2231 2263 2231 Size: 32 5339 PstI C_TGCA'G Cuts at: 830 2723 830 Size: 1893 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5371 SacI G_AGCT'C Cuts at: 2279 2279 Size: 5371 SalI G'TCGA_C Cuts at: 1936 2713 1936 Size: 777 4594 SbfI CC_TGCA'GG Cuts at: 2723 2723 Size: 5371 ScaI AGT'ACT Cuts at: 2531 3619 5331 2531 Size: 1088 1712 2571 Fragments arranged by size: 2571 1712 1088 SfcI C'TryA_G Cuts at: 826 1045 2719 3854 4535 4726 826 Size: 219 1674 1135 681 191 1471 Fragments arranged by size: 1674 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5371 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5371 SmaI CCC'GGG Cuts at: 1872 2230 2698 1872 Size: 358 468 4545 Fragments arranged by size: 4545 468 358 SmlI C'TyrA_G Cuts at: 820 1017 1943 3475 4376 4638 820 Size: 197 926 1532 901 262 1553 Fragments arranged by size: 1553 1532 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3162 493 Size: 2669 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5371 SphI G_CATG'C Cuts at: 1132 2729 1132 Size: 1597 3774 SspI AAT'ATT Cuts at: 5 52 3295 5 Size: 47 3243 2081 Fragments arranged by size: 3243 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5371 StyI C'CwwG_G Cuts at: 513 1065 2190 2347 2636 2810 513 Size: 552 1125 157 289 174 3074 Fragments arranged by size: 3074 1125 552 289 174 157 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3398 3415 3568 3753 4198 325 Size: 1255 1818 17 153 185 445 1498 Fragments arranged by size: 1818 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2310 2529 3617 Size: 274 80 33 51 1443 333 219 1088 Cuts at: 3617 5329 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1088 333 274 219 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5331 5363 5331 Size: 32 5339 Tth111I GACn'n_nGTC Cuts at: 1316 2516 1316 Size: 1200 4171 XbaI T'CTAG_A Cuts at: 2707 2707 Size: 5371 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2080 2080 Size: 5371 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5371 XmaI C'CCGG_G Cuts at: 1870 2228 2696 1870 Size: 358 468 4545 Fragments arranged by size: 4545 468 358 XmnI GAAnn'nnTTC Cuts at: 3500 3500 Size: 5371 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3179 276 Size: 53 83 186 936 1645 2468 Fragments arranged by size: 2468 1645 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PsrI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI