################################## # NO CUT ENZYMES ( 207 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Cac8I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoO65I EcoRV EcoT14I EcoT22I FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII HphI KasI KpnI Ksp632I KspI MaeI MaeIII MamI MfeI MluI MluNI MroI MscI MseI MslI MspI MunI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIII NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI Tru9I Tsp45I TspRI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AciI 94 GCGG CCGC(-3/-1) AcsI 77 GAATTC [GA]^AATT[CT] AfaI 114 GTAC GT^AC AluI 50 AGCT AG^CT AocI 160 CCTGAGG CC^T[ACGT]AGG ApoI 77 GAATTC [GA]^AATT[CT] Asp700I 171 GAATCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaII 24 GGACC G^G[AT]CC BbvI 48 GCAGC GCAGC(8/12) BsaAI 115 TACGTG [CT]AC^GT[GA] BsaJI 5 CCCAGG C^C[ACGT][ACGT]GG BssKI 6 CCAGG ^CC[ACGT]GG BstF5I 156 CATCC GGATG(2/0) BstNI 6 CCAGG CC^[AT]GG BstYI 9 GGATCT [GA]^GATC[CT] Bsu36I 160 CCTGAGG CC^T[ACGT]AGG DraII 23 AGGACCT [GA]G^G[ACGT]CC[CT] Eco81I 160 CCTGAGG CC^T[ACGT]AGG EcoO109I 23 AGGACCT [GA]G^G[ACGT]CC[CT] EcoRI 77 GAATTC G^AATTC EcoRII 6 CCAGG ^CC[AT]GG FauI 94 GCGGG CCCGC(4/6) Fnu4HI 48 GCAGC GC^[ACGT]GC FokI 156 CATCC GGATG(9/13) HinfI 171 GAATC G^A[ACGT]TC ItaI 48 GCAGC GC^[ACGT]GC MaeII 116 ACGT A^CGT MflI 9 GGATCT [GA]^GATC[CT] MspA1I 49 CAGCTG C[AC]G^C[GT]G MvaI 6 CCAGG CC^[AT]GG PpuMI 23 AGGACCT [GA]G^G[AT]CC[CT] PvuII 49 CAGCTG CAG^CTG RsaI 114 GTAC GT^AC ScrFI 6 CCAGG CC^[ACGT]GG SfaNI 155 GCATC GCATC(5/9) TfiI 171 GAATC G^A[AT]TC TseI 48 GCAGC G^C[AT]GC Tsp509I 78 AATT ^AATT XhoII 9 GGATCT [GA]^GATC[CT] XmnI 171 GAATCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 8 enzymes) # ################################### AlwI 139 GATCC GGATC(4/5) 9 GGATC DdeI 13 CTCAG C^T[ACGT]AG 161 CTGAG DpnII 10 GATC ^GATC 139 GATC HaeIII 3 GGCC GG^CC 123 GGCC MboI 10 GATC ^GATC 139 GATC MboII 33 GAAGA GAAGA(8/7) 54 GAAGA NdeII 10 GATC ^GATC 139 GATC Sau3AI 10 GATC ^GATC 139 GATC ######################################### # 3 OR MORE CUT ENZYMES ( 3 enzymes) # ######################################### Cfr13I MnlI Sau96I