Restriction map for
pFN21AB7819
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# NO CUT ENZYMES ( 121 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwI AlwNI AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
AspEI AviII AvrII BamHI BbrPI
BbsI BbvCI BcgI BfaI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaWI BseAI BsiWI BsmBI
BspDI BspEI BspHI BspLU11I BspMI
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstYI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraIII Eam1105I
EarI Eco47III Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HindIII HpaI Ksp632I MaeI MboII
MfeI MflI MluI MroI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PstI PvuI RcaI RsrII SacI
SalI SapI SbfI ScaI SexAI
SfiI SfuI SgrAI SmlI SnaBI
SpeI Sse8387I SspI StuI SwaI
Van91I XbaI XcmI XhoI XhoII
XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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Acc65I 593 GGTACC G^GTACC
Asp718I 593 GGTACC G^GTACC
AspHI 775 GTGCTC G[AT]GC[AT]^C
AspI 562 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1122 TGGCCA TGG^CCA
BciVI 1086 GTATCC GTATCC(6/5)
BclI 470 TGATCA T^GATCA
BglI 1376 GCCCTGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 468 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 164 GGTCTC GGTCTC(1/5)
BsgI 613 GTGCAG GTGCAG(16/14)
BsiHKAI 775 GTGCTC G[AT]GC[AT]^C
Bsp1407I 992 TGTACA T^GTACA
BsrBI 600 GAGCGG CCGCTC(-3/-3)
BsrDI 897 CATTGC GCAATG(2/0)
BsrFI 250 GCCGGC [GA]^CCGG[CT]
BsrGI 992 TGTACA T^GTACA
BstAPI 690 GCACTGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 250 GCCGGC [GA]^CCGG[CT]
DrdI 1031 GACCCAACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 470 TGATCA T^GATCA
HincII 1003 GTTGAC GT[CT]^[GA]AC
HindII 1003 GTTGAC GT[CT]^[GA]AC
KpnI 593 GGTACC GGTAC^C
MamI 468 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1122 TGGCCA TGG^CCA
MscI 1122 TGGCCA TGG^CCA
MseI 449 TTAA T^TAA
NaeI 250 GCCGGC GCC^GGC
NcoI 1364 CCATGG C^CATGG
NgoMIV 250 GCCGGC G^CCGGC
PflFI 562 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
SfcI 546 CTACAG C^T[GA][CT]AG
SphI 638 GCATGC GCATG^C
SspBI 992 TGTACA T^GTACA
TaqI 1197 TCGA T^CGA
Tru9I 449 TTAA T^TAA
Tth111I 562 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1197 TCGA T^CGA
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
BsiEI 248 CGGCCG CG[GA][CT]^CG
1450 CGGCCG
BsmI 918 GAATGC GAATGC(1/-1)
1219 GCATTC
BssHII 122 GCGCGC G^CGCGC
339 GCGCGC
DpnII 471 GATC ^GATC
1057 GATC
EagI 248 CGGCCG C^GGCCG
1450 CGGCCG
EclXI 248 CGGCCG C^GGCCG
1450 CGGCCG
Eco52I 248 CGGCCG C^GGCCG
1450 CGGCCG
KspI 287 CCGCGG CCGC^GG
383 CCGCGG
MaeII 563 ACGT A^CGT
659 ACGT
MboI 471 GATC ^GATC
1057 GATC
MslI 897 CATTGCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1177 CACAGCTATG
NdeII 471 GATC ^GATC
1057 GATC
NspI 29 ACATGC [GA]CATG^[CT]
638 GCATGC
PpuMI 1271 AGGTCCT [GA]G^G[AT]CC[CT]
1413 GGGTCCC
PshAI 136 GACGAGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1408 GACCAGGGTC
PvuII 934 CAGCTG CAG^CTG
1335 CAGCTG
SacII 287 CCGCGG CCGC^GG
383 CCGCGG
Sau3AI 471 GATC ^GATC
1057 GATC
Tsp509I 942 AATT ^AATT
1043 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
ApaI AvaI AvaII BanI BanII
BbeI BbvI BcnI BmyI BpmI
BsaHI BsaJI BseRI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DraII DsaI
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI MaeIII
MnlI MspA1I MspI MvaI MvnI
MwoI NarI NciI NlaIII NlaIV
PleI RsaI Sau96I ScrFI SfaNI
SfoI SmaI StyI TfiI TseI
Tsp45I TspRI XmaCI XmaI