(Circular) (Six-base) MAPSORT of: pFN21AB7819.seq Check: 7406 from: 1 to: 6310 pFN21AB7819 6310 bp With 182 enzymes: SgfI * October 25, 2019 12:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4120 278 Size: 53 83 186 936 2584 2468 Fragments arranged by size: 2584 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3653 1937 Size: 1716 4594 Acc65I G'GTAC_C Cuts at: 2598 3631 2598 Size: 1033 5277 AclI AA'CG_TT Cuts at: 1563 3609 4437 4810 1563 Size: 2046 828 373 3063 Fragments arranged by size: 3063 2046 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3600 4373 5757 6025 1632 Size: 1968 773 1384 268 1917 Fragments arranged by size: 1968 1917 1384 773 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6113 AflIII A'CryG_T Cuts at: 1203 5209 6186 1203 Size: 4006 977 1327 Fragments arranged by size: 4006 1327 977 AgeI A'CCGG_T Cuts at: 5141 5141 Size: 6310 AhdI GACnn_n'nnGTC Cuts at: 5039 5039 Size: 6310 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6310 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2633 1184 Size: 1449 4861 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2975 3007 2975 Size: 32 6278 AlwNI CAG_nnn'CTG Cuts at: 4053 5625 4053 Size: 1572 4738 ApaI G_GGCC'C Cuts at: 3172 3249 3335 3556 3172 Size: 77 86 221 5926 Fragments arranged by size: 5926 221 86 77 ApaLI G'TGCA_C Cuts at: 4367 5523 4367 Size: 1156 5154 ApoI r'AATT_y Cuts at: 1493 3619 3845 3997 6106 1493 Size: 2126 226 152 2109 1697 Fragments arranged by size: 2126 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4864 160 Size: 872 3832 1606 Fragments arranged by size: 3832 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6310 AvaI C'yCGr_G Cuts at: 1870 1943 2179 2359 3174 3635 1870 Size: 73 236 180 815 461 4545 Fragments arranged by size: 4545 815 461 236 180 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3299 3332 3299 Size: 33 6277 BamHI G'GATC_C Cuts at: 3640 4007 3640 Size: 367 5943 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2045 2080 2087 2186 2194 Size: 325 217 627 258 35 7 99 8 Cuts at: 2194 2598 3283 3307 3631 5086 618 Size: 404 685 24 324 1455 1842 Fragments arranged by size: 1842 1455 685 627 404 325 324 258 217 99 35 24 8 7 BanII G_rGCy'C Cuts at: 1391 2355 2541 3172 3249 3335 3556 3629 1391 Size: 964 186 631 77 86 221 73 4072 Fragments arranged by size: 4072 964 631 221 186 86 77 73 BbeI G_GCGC'C Cuts at: 2084 2091 2190 2084 Size: 7 99 6204 Fragments arranged by size: 6204 99 7 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5339 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4501 4535 1903 Size: 34 2564 34 3678 Fragments arranged by size: 3678 2564 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3102 4202 5412 3102 Size: 1100 1210 4000 Fragments arranged by size: 4000 1210 1100 BclI T'GATC_A Cuts at: 1548 2475 1548 Size: 927 5383 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3387 4921 136 Size: 107 122 71 631 605 1715 1534 1525 Fragments arranged by size: 1715 1534 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6305 6305 Size: 6310 BlpI GC'TnA_GC Cuts at: 3727 3727 Size: 6310 Bme1580I G_kGCm'C Cuts at: 1790 2048 2199 3172 3249 3335 3556 4371 5527 Size: 258 151 973 77 86 221 815 1156 Cuts at: 5527 1790 Size: 2573 Fragments arranged by size: 2573 1156 973 815 258 221 151 86 77 BmgBI CAC'GTC Cuts at: 1318 2569 1318 Size: 1251 5059 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2890 3307 3488 Size: 416 53 83 399 24 1473 417 181 Cuts at: 3488 4041 4999 442 Size: 553 958 1753 Fragments arranged by size: 1753 1473 958 553 417 416 399 181 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6310 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2417 2555 2768 3309 3405 4970 1239 Size: 1178 138 213 541 96 1565 2579 Fragments arranged by size: 2579 1565 1178 541 213 138 96 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4435 5300 5598 4435 Size: 865 298 5147 Fragments arranged by size: 5147 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2175 4973 882 Size: 617 676 2798 2219 Fragments arranged by size: 2798 2219 676 617 BsaAI yAC'GTr Cuts at: 493 1204 4101 493 Size: 711 2897 2702 Fragments arranged by size: 2897 2702 711 BsaBI GATnn'nnATC Cuts at: 2477 6304 2477 Size: 3827 2483 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2081 2088 2187 2298 Size: 53 83 186 936 548 7 99 111 Cuts at: 2298 2753 4117 4499 5134 275 Size: 455 1364 382 635 1451 Fragments arranged by size: 1451 1364 936 635 548 455 382 186 111 99 83 53 7 BsaWI w'CCGG_w Cuts at: 4743 5141 5415 5562 5973 4743 Size: 398 274 147 411 5080 Fragments arranged by size: 5080 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2660 2690 3524 3554 1178 Size: 30 165 30 1257 30 834 30 3934 Fragments arranged by size: 3934 1257 834 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3063 3514 3529 1186 Size: 1877 451 15 3967 Fragments arranged by size: 3967 1877 451 15 BseYI C'CCAG_C Cuts at: 3101 3163 3548 5513 6009 3101 Size: 62 385 1965 496 3402 Fragments arranged by size: 3402 1965 496 385 62 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2639 1163 Size: 573 903 4834 Fragments arranged by size: 4834 903 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2256 3458 3760 4521 5549 1540 Size: 275 186 255 1202 302 761 1028 2301 Fragments arranged by size: 2301 1202 1028 761 302 275 255 186 BsiHKAI G_wGCw'C Cuts at: 2784 4371 4456 5527 2784 Size: 1587 85 1071 3567 Fragments arranged by size: 3567 1587 1071 85 BsmI GAATG_Cn' Cuts at: 2929 3224 3821 3914 2929 Size: 295 597 93 5325 Fragments arranged by size: 5325 597 295 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2048 2199 2355 2541 2784 3172 3249 Size: 399 258 151 156 186 243 388 77 Cuts at: 3249 3335 3556 3629 4371 4456 5527 1391 Size: 86 221 73 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 388 258 243 221 186 156 151 86 85 77 73 BspEI T'CCGG_A Cuts at: 5973 5973 Size: 6310 BspHI T'CATG_A Cuts at: 4199 5929 4199 Size: 1730 4580 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3665 844 Size: 2821 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2607 4197 1394 Size: 429 784 1590 3507 Fragments arranged by size: 3507 1590 784 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2901 4805 4979 65 Size: 1183 1653 1904 174 1396 Fragments arranged by size: 1904 1653 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2255 4954 5141 1700 Size: 110 445 2699 187 2869 Fragments arranged by size: 2869 2699 445 187 110 BsrGI T'GTAC_A Cuts at: 96 2997 96 Size: 2901 3409 BssHII G'CGCG_C Cuts at: 1925 2127 2344 1925 Size: 202 217 5891 Fragments arranged by size: 5891 217 202 BssSI C'ACGA_G Cuts at: 4370 5382 4370 Size: 1012 5298 BstAPI GCAn_nnn'nTGC Cuts at: 794 2701 794 Size: 1907 4403 BstBI TT'CG_AA Cuts at: 5114 5114 Size: 6310 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6310 BstYI r'GATC_y Cuts at: 1972 3640 4007 4392 4409 5861 5947 5959 6305 Size: 1668 367 385 17 1452 86 12 346 Cuts at: 6305 1972 Size: 1977 Fragments arranged by size: 1977 1668 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2292 2388 3369 513 Size: 552 939 288 96 981 3454 Fragments arranged by size: 3454 981 939 552 288 96 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5571 Fragments arranged by size: 5571 683 56 BtsI GCAGTG_nn' Cuts at: 2627 3826 4620 4640 2627 Size: 1199 794 20 4297 Fragments arranged by size: 4297 1199 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6310 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6275 DraI TTT'AAA Cuts at: 3614 3614 Size: 6310 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3042 5317 809 Size: 507 1726 2275 1802 Fragments arranged by size: 2275 1802 1726 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2253 2526 3127 Size: 54 1425 50 275 18 423 273 601 Cuts at: 3127 3336 3455 3757 4646 6275 8 Size: 209 119 302 889 1629 43 Fragments arranged by size: 1629 1425 889 601 423 302 275 273 209 119 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2253 3455 3757 1537 Size: 275 441 1202 302 4090 Fragments arranged by size: 4090 1202 441 302 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4240 1353 Size: 2887 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2512 3338 4897 5271 5417 387 Size: 1064 138 923 826 1559 374 146 1280 Fragments arranged by size: 1559 1280 1064 923 826 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2417 2555 2768 3309 3405 3600 4373 Size: 393 785 138 213 541 96 195 773 Cuts at: 4373 4970 5757 6025 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 787 785 773 597 541 393 268 213 195 138 96 EcoO109I rG'GnC_Cy Cuts at: 3168 3169 3277 3419 3552 3553 3168 Size: 1 108 142 133 1 5925 Fragments arranged by size: 5925 142 133 108 1 1 EcoRI G'AATT_C Cuts at: 3619 3997 3619 Size: 378 5932 FspI TGC'GCA Cuts at: 4816 4816 Size: 6310 HaeII r_GCGC'y Cuts at: 1678 2084 2091 2190 5457 1678 Size: 406 7 99 3267 2531 Fragments arranged by size: 3267 2531 406 99 7 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3053 3085 3524 3556 4951 Size: 32 135 32 1481 32 439 32 1395 Cuts at: 4951 4983 5025 5057 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1481 1395 439 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3010 3654 3900 669 Size: 988 281 1072 644 246 3079 Fragments arranged by size: 3079 1072 988 644 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6310 HpaI GTT'AAC Cuts at: 3900 3900 Size: 6310 KasI G'GCGC_C Cuts at: 2080 2087 2186 2080 Size: 7 99 6204 Fragments arranged by size: 6204 99 7 KpnI G_GTAC'C Cuts at: 2602 3635 2602 Size: 1033 5277 MfeI C'AATT_G Cuts at: 3909 3909 Size: 6310 MluI A'CGCG_T Cuts at: 6186 6186 Size: 6310 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2781 2889 5424 5608 1305 Size: 1476 108 2535 184 2007 Fragments arranged by size: 2535 2007 1476 184 108 MscI TGG'CCA Cuts at: 10 64 1489 1832 3129 10 Size: 54 1425 343 1297 3191 Fragments arranged by size: 3191 1425 1297 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2906 3186 4268 4627 4786 518 Size: 648 1740 280 1082 359 159 2042 Fragments arranged by size: 2042 1740 1082 648 359 280 159 MspA1I CmG'CkG Cuts at: 1606 2294 2390 2941 3342 3726 4403 5551 5796 Size: 688 96 551 401 384 677 1148 245 Cuts at: 5796 1606 Size: 2120 Fragments arranged by size: 2120 1148 688 677 551 401 384 245 96 NaeI GCC'GGC Cuts at: 2257 2257 Size: 6310 NarI GG'CG_CC Cuts at: 2081 2088 2187 2081 Size: 7 99 6204 Fragments arranged by size: 6204 99 7 NcoI C'CATG_G Cuts at: 513 1065 2004 3369 513 Size: 552 939 1365 3454 Fragments arranged by size: 3454 1365 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6310 NgoMIV G'CCGG_C Cuts at: 2255 2255 Size: 6310 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6310 NotI GC'GGCC_GC Cuts at: 3757 3757 Size: 6310 NspI r_CATG'y Cuts at: 1132 1229 2038 2647 3668 5213 1132 Size: 97 809 609 1021 1545 2229 Fragments arranged by size: 2229 1545 1021 809 609 97 PasI CC'CwG_GG Cuts at: 1348 3242 1348 Size: 1894 4416 PciI A'CATG_T Cuts at: 5209 5209 Size: 6310 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6310 PfoI T'CCnGG_A Cuts at: 2976 2976 Size: 6310 PmeI GTTT'AAAC Cuts at: 3614 3614 Size: 6310 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4379 4411 5892 5924 4379 Size: 32 1481 32 4765 Fragments arranged by size: 4765 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3277 3419 3277 Size: 142 6168 PshAI GACnn'nnGTC Cuts at: 1613 2145 3417 1613 Size: 532 1272 4506 Fragments arranged by size: 4506 1272 532 PsiI TTA'TAA Cuts at: 3594 3880 3594 Size: 286 6024 PspOMI G'GGCC_C Cuts at: 3168 3245 3331 3552 3168 Size: 77 86 221 5926 Fragments arranged by size: 5926 221 86 77 PstI C_TGCA'G Cuts at: 830 3662 830 Size: 2832 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6310 PvuII CAG'CTG Cuts at: 2941 3342 2941 Size: 401 5909 SacII CC_GC'GG Cuts at: 2295 2391 2295 Size: 96 6214 SalI G'TCGA_C Cuts at: 1936 3652 1936 Size: 1716 4594 SanDI GG'GwC_CC Cuts at: 3419 3419 Size: 6310 SbfI CC_TGCA'GG Cuts at: 3662 3662 Size: 6310 ScaI AGT'ACT Cuts at: 4558 6270 4558 Size: 1712 4598 SfcI C'TryA_G Cuts at: 826 1045 2551 3658 4793 5474 5665 826 Size: 219 1506 1107 1135 681 191 1471 Fragments arranged by size: 1506 1471 1135 1107 681 219 191 SfoI GGC'GCC Cuts at: 2082 2089 2188 2082 Size: 7 99 6204 Fragments arranged by size: 6204 99 7 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6310 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6310 SmaI CCC'GGG Cuts at: 1872 2181 2361 3176 3637 1872 Size: 309 180 815 461 4545 Fragments arranged by size: 4545 815 461 309 180 SmlI C'TyrA_G Cuts at: 820 1017 1943 4414 5315 5577 820 Size: 197 926 2471 901 262 1553 Fragments arranged by size: 2471 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4101 493 Size: 3608 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6310 SphI G_CATG'C Cuts at: 1132 2647 3668 1132 Size: 1515 1021 3774 Fragments arranged by size: 3774 1515 1021 SspI AAT'ATT Cuts at: 5 52 4234 5 Size: 47 4182 2081 Fragments arranged by size: 4182 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6310 StyI C'CwwG_G Cuts at: 513 1065 2004 2190 2218 3052 3369 3426 3749 Size: 552 939 186 28 834 317 57 323 Cuts at: 3749 513 Size: 3074 Fragments arranged by size: 3074 939 834 552 323 317 186 57 28 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2605 4337 4354 4507 4692 5137 325 Size: 1255 1025 1732 17 153 185 445 1498 Fragments arranged by size: 1732 1498 1255 1025 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2997 4556 6268 Size: 274 80 33 51 1443 1020 1559 1712 Cuts at: 6268 96 Size: 138 Fragments arranged by size: 1712 1559 1443 1020 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2660 2692 6270 6302 2660 Size: 32 3578 32 2668 Fragments arranged by size: 3578 2668 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2570 1316 Size: 1254 5056 XbaI T'CTAG_A Cuts at: 3646 3646 Size: 6310 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 6310 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6310 XmaI C'CCGG_G Cuts at: 1870 2179 2359 3174 3635 1870 Size: 309 180 815 461 4545 Fragments arranged by size: 4545 815 461 309 180 XmnI GAAnn'nnTTC Cuts at: 4439 4439 Size: 6310 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4118 276 Size: 53 83 186 936 2584 2468 Fragments arranged by size: 2584 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SapI SexAI SfiI SrfI SwaI