Restriction map for
pFN21AB7819
(Linear) (Six-base) MAPSORT of: KIBB7819.seq Check: 3151 from: 1 to: 1605
KIBB7819.orf
With 182 enzymes: SgfI *
October 24, 2019 20:49 ..
Acc65I G'GTAC_C
Cuts at: 0 593 1605
Size: 593 1012
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1595 1605
Size: 1595 10
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 628 1605
Size: 628 977
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 970 1002 1605
Size: 970 32 603
Fragments arranged by size:
970 603 32
ApaI G_GGCC'C
Cuts at: 0 1167 1244 1330 1551 1605
Size: 1167 77 86 221 54
Fragments arranged by size:
1167 221 86 77 54
AvaI C'yCGr_G
Cuts at: 0 174 354 1169 1605
Size: 174 180 815 436
Fragments arranged by size:
815 436 180 174
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1294 1327 1605
Size: 1294 33 278
Fragments arranged by size:
1294 278 33
BanI G'GyrC_C
Cuts at: 0 40 75 82 181 189 593 1278 1302
Size: 40 35 7 99 8 404 685 24
Cuts at: 1302 1605
Size: 303
Fragments arranged by size:
685 404 303 99 40 35 24 8
7
BanII G_rGCy'C
Cuts at: 0 350 536 1167 1244 1330 1551 1605
Size: 350 186 631 77 86 221 54
Fragments arranged by size:
631 350 221 186 86 77 54
BbeI G_GCGC'C
Cuts at: 0 79 86 185 1605
Size: 79 7 99 1420
Fragments arranged by size:
1420 99 79 7
BciVI GTATCCnnnnn_n'
Cuts at: 0 1097 1605
Size: 1097 508
BclI T'GATC_A
Cuts at: 0 470 1605
Size: 470 1135
BglI GCCn_nnn'nGGC
Cuts at: 0 1382 1605
Size: 1382 223
Bme1580I G_kGCm'C
Cuts at: 0 43 194 1167 1244 1330 1551 1605
Size: 43 151 973 77 86 221 54
Fragments arranged by size:
973 221 151 86 77 54 43
BmgBI CAC'GTC
Cuts at: 0 564 1605
Size: 564 1041
BmrI ACTGGGnnnn_n'
Cuts at: 0 885 1302 1483 1605
Size: 885 417 181 122
Fragments arranged by size:
885 417 181 122
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 412 550 763 1304 1400 1605
Size: 412 138 213 541 96 205
Fragments arranged by size:
541 412 213 205 138 96
BsaI GGTCTCn'nnnn_
Cuts at: 0 170 1605
Size: 170 1435
BsaBI GATnn'nnATC
Cuts at: 0 472 1605
Size: 472 1133
BsaHI Gr'CG_yC
Cuts at: 0 76 83 182 293 748 1605
Size: 76 7 99 111 455 857
Fragments arranged by size:
857 455 111 99 76 7
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 655 685 1519 1549 1605
Size: 655 30 834 30 56
Fragments arranged by size:
834 655 56 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1058 1509 1524 1605
Size: 1058 451 15 81
Fragments arranged by size:
1058 451 81 15
BseYI C'CCAG_C
Cuts at: 0 1096 1158 1543 1605
Size: 1096 62 385 62
Fragments arranged by size:
1096 385 62 62
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 634 1605
Size: 634 971
BsiEI CG_ry'CG
Cuts at: 0 251 1453 1605
Size: 251 1202 152
Fragments arranged by size:
1202 251 152
BsiHKAI G_wGCw'C
Cuts at: 0 779 1605
Size: 779 826
BsmI GAATG_Cn'
Cuts at: 0 924 1219 1605
Size: 924 295 386
Fragments arranged by size:
924 386 295
Bsp1286I G_dGCh'C
Cuts at: 0 43 194 350 536 779 1167 1244 1330
Size: 43 151 156 186 243 388 77 86
Cuts at: 1330 1551 1605
Size: 221 54
Fragments arranged by size:
388 243 221 186 156 151 86 77
54 43
BsrBI CCG'CTC
Cuts at: 0 602 1605
Size: 602 1003
BsrDI GCAATG_nn'
Cuts at: 0 896 1605
Size: 896 709
BsrFI r'CCGG_y
Cuts at: 0 250 1605
Size: 250 1355
BsrGI T'GTAC_A
Cuts at: 0 992 1605
Size: 992 613
BssHII G'CGCG_C
Cuts at: 0 122 339 1605
Size: 122 217 1266
Fragments arranged by size:
1266 217 122
BstAPI GCAn_nnn'nTGC
Cuts at: 0 696 1605
Size: 696 909
BtgI C'CryG_G
Cuts at: 0 287 383 1364 1605
Size: 287 96 981 241
Fragments arranged by size:
981 287 241 96
BtsI GCAGTG_nn'
Cuts at: 0 622 1605
Size: 622 983
DrdI GACnn_nn'nnGTC
Cuts at: 0 1037 1605
Size: 1037 568
EaeI y'GGCC_r
Cuts at: 0 248 521 1122 1331 1450 1605
Size: 248 273 601 209 119 155
Fragments arranged by size:
601 273 248 209 155 119
EagI C'GGCC_G
Cuts at: 0 248 1450 1605
Size: 248 1202 155
Fragments arranged by size:
1202 248 155
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 507 1333 1605
Size: 507 826 272
Fragments arranged by size:
826 507 272
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 412 550 763 1304 1400 1595 1605
Size: 412 138 213 541 96 195 10
Fragments arranged by size:
541 412 213 195 138 96 10
EcoO109I rG'GnC_Cy
Cuts at: 0 1163 1164 1272 1414 1547 1548 1605
Size: 1163 1 108 142 133 1 57
Fragments arranged by size:
1163 142 133 108 57 1 1
HaeII r_GCGC'y
Cuts at: 0 79 86 185 1605
Size: 79 7 99 1420
Fragments arranged by size:
1420 99 79 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1048 1080 1519 1551 1605
Size: 1048 32 439 32 54
Fragments arranged by size:
1048 439 54 32 32
HincII GTy'rAC
Cuts at: 0 1005 1605
Size: 1005 600
KasI G'GCGC_C
Cuts at: 0 75 82 181 1605
Size: 75 7 99 1424
Fragments arranged by size:
1424 99 75 7
KpnI G_GTAC'C
Cuts at: 0 597 1605
Size: 597 1008
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 776 884 1605
Size: 776 108 721
Fragments arranged by size:
776 721 108
MscI TGG'CCA
Cuts at: 0 1124 1605
Size: 1124 481
MslI CAynn'nnrTG
Cuts at: 0 901 1181 1605
Size: 901 280 424
Fragments arranged by size:
901 424 280
MspA1I CmG'CkG
Cuts at: 0 289 385 936 1337 1605
Size: 289 96 551 401 268
Fragments arranged by size:
551 401 289 268 96
NaeI GCC'GGC
Cuts at: 0 252 1605
Size: 252 1353
NarI GG'CG_CC
Cuts at: 0 76 83 182 1605
Size: 76 7 99 1423
Fragments arranged by size:
1423 99 76 7
NcoI C'CATG_G
Cuts at: 0 1364 1605
Size: 1364 241
NgoMIV G'CCGG_C
Cuts at: 0 250 1605
Size: 250 1355
NspI r_CATG'y
Cuts at: 0 33 642 1605
Size: 33 609 963
Fragments arranged by size:
963 609 33
PasI CC'CwG_GG
Cuts at: 0 1237 1605
Size: 1237 368
PfoI T'CCnGG_A
Cuts at: 0 971 1605
Size: 971 634
PpuMI rG'GwC_Cy
Cuts at: 0 1272 1414 1605
Size: 1272 142 191
Fragments arranged by size:
1272 191 142
PshAI GACnn'nnGTC
Cuts at: 0 140 1412 1605
Size: 140 1272 193
Fragments arranged by size:
1272 193 140
PsiI TTA'TAA
Cuts at: 0 1589 1605
Size: 1589 16
PspOMI G'GGCC_C
Cuts at: 0 1163 1240 1326 1547 1605
Size: 1163 77 86 221 58
Fragments arranged by size:
1163 221 86 77 58
PvuII CAG'CTG
Cuts at: 0 936 1337 1605
Size: 936 401 268
Fragments arranged by size:
936 401 268
SacII CC_GC'GG
Cuts at: 0 290 386 1605
Size: 290 96 1219
Fragments arranged by size:
1219 290 96
SanDI GG'GwC_CC
Cuts at: 0 1414 1605
Size: 1414 191
SfcI C'TryA_G
Cuts at: 0 546 1605
Size: 546 1059
SfoI GGC'GCC
Cuts at: 0 77 84 183 1605
Size: 77 7 99 1422
Fragments arranged by size:
1422 99 77 7
SmaI CCC'GGG
Cuts at: 0 176 356 1171 1605
Size: 176 180 815 434
Fragments arranged by size:
815 434 180 176
SphI G_CATG'C
Cuts at: 0 642 1605
Size: 642 963
StyI C'CwwG_G
Cuts at: 0 185 213 1047 1364 1421 1605
Size: 185 28 834 317 57 184
Fragments arranged by size:
834 317 185 184 57 28
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 600 1605
Size: 600 1005
TatI w'GTAC_w
Cuts at: 0 992 1605
Size: 992 613
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 655 687 1605
Size: 655 32 918
Fragments arranged by size:
918 655 32
Tth111I GACn'n_nGTC
Cuts at: 0 565 1605
Size: 565 1040
XmaI C'CCGG_G
Cuts at: 0 174 354 1169 1605
Size: 174 180 815 436
Fragments arranged by size:
815 436 180 174
Enzymes that do cut:
Acc65I AcuI AlfI AloI ApaI AvaI BaeI BanI
BanII BbeI BciVI BclI BglI Bme1580I BmgBI BmrI
BpmI BsaI BsaBI BsaHI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BsrBI BsrDI BsrFI BsrGI
BssHII BstAPI BtgI BtsI DrdI EaeI EagI EciI
Eco57MI EcoO109I HaeII Hin4I HincII KasI KpnI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PfoI PpuMI PshAI PsiI PspOMI PvuII SacII
SanDI SfcI SfoI SmaI SphI StyI TaqII TatI
TstI Tth111I XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AleI AlwNI ApaLI ApoI AscI AseI AsiSI
AvrII BamHI BbsI BbvCI BcgI BfrBI BglII BlpI
BmtI BplI Bpu10I BpuEI BsaAI BsaWI BsiWI BsmBI
BspEI BspHI BspMI BssSI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII EarI
EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HindIII HpaI MfeI MluI NdeI NheI NotI NruI
NsiI PacI PciI PflMI PmeI PmlI PpiI PspXI
PsrI PstI PvuI RsrII SacI SalI SapI SbfI
ScaI SexAI SfiI SgfI SgrAI SmlI SnaBI SpeI
SrfI SspI StuI SwaI XbaI XcmI XhoI XmnI
ZraI