Restriction map for
pFN21AE5318
(Linear) (Six-base) MAPSORT of: KIEE5318.seq Check: 1273 from: 1 to: 858
KIEE5318.orf
With 182 enzymes: SgfI *
December 5, 2014 13:01 ..
Acc65I G'GTAC_C
Cuts at: 0 853 858
Size: 853 5
AlwNI CAG_nnn'CTG
Cuts at: 0 438 636 708 858
Size: 438 198 72 150
Fragments arranged by size:
438 198 150 72
ApaLI G'TGCA_C
Cuts at: 0 717 858
Size: 717 141
AvaI C'yCGr_G
Cuts at: 0 78 374 858
Size: 78 296 484
Fragments arranged by size:
484 296 78
BanI G'GyrC_C
Cuts at: 0 73 853 858
Size: 73 780 5
Fragments arranged by size:
780 73 5
BanII G_rGCy'C
Cuts at: 0 88 229 467 858
Size: 88 141 238 391
Fragments arranged by size:
391 238 141 88
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 812 846 858
Size: 812 34 12
Fragments arranged by size:
812 34 12
BglI GCCn_nnn'nGGC
Cuts at: 0 233 617 858
Size: 233 384 241
Fragments arranged by size:
384 241 233
BglII A'GATC_T
Cuts at: 0 168 858
Size: 168 690
BlpI GC'TnA_GC
Cuts at: 0 240 322 858
Size: 240 82 536
Fragments arranged by size:
536 240 82
Bme1580I G_kGCm'C
Cuts at: 0 78 482 721 858
Size: 78 404 239 137
Fragments arranged by size:
404 239 137 78
BmrI ACTGGGnnnn_n'
Cuts at: 0 426 858
Size: 426 432
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 171 858
Size: 171 687
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 337 673 679 858
Size: 73 264 336 6 179
Fragments arranged by size:
336 264 179 73 6
Bpu10I CC'TnA_GC
Cuts at: 0 101 858
Size: 101 757
BsaBI GATnn'nnATC
Cuts at: 0 167 858
Size: 167 691
BsaWI w'CCGG_w
Cuts at: 0 776 858
Size: 776 82
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 70 475 697 700 763 858
Size: 70 405 222 3 63 95
Fragments arranged by size:
405 222 95 70 63 3
BseYI C'CCAG_C
Cuts at: 0 175 326 858
Size: 175 151 532
Fragments arranged by size:
532 175 151
BsiHKAI G_wGCw'C
Cuts at: 0 721 858
Size: 721 137
Bsp1286I G_dGCh'C
Cuts at: 0 78 88 229 467 482 721 858
Size: 78 10 141 238 15 239 137
Fragments arranged by size:
239 238 141 137 78 15 10
BspEI T'CCGG_A
Cuts at: 0 776 858
Size: 776 82
BspHI T'CATG_A
Cuts at: 0 108 858
Size: 108 750
BsrFI r'CCGG_y
Cuts at: 0 721 858
Size: 721 137
BstAPI GCAn_nnn'nTGC
Cuts at: 0 708 858
Size: 708 150
BstYI r'GATC_y
Cuts at: 0 168 488 858
Size: 168 320 370
Fragments arranged by size:
370 320 168
BtgI C'CryG_G
Cuts at: 0 139 410 483 858
Size: 139 271 73 375
Fragments arranged by size:
375 271 139 73
EaeI y'GGCC_r
Cuts at: 0 5 280 723 858
Size: 5 275 443 135
Fragments arranged by size:
443 275 135 5
EarI CTCTTCn'nnn_
Cuts at: 0 380 858
Size: 380 478
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 694 858
Size: 694 164
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 337 673 679 858
Size: 73 264 336 6 179
Fragments arranged by size:
336 264 179 73 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 55 858
Size: 55 803
EcoO109I rG'GnC_Cy
Cuts at: 0 368 453 858
Size: 368 85 405
Fragments arranged by size:
405 368 85
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 26 858
Size: 26 832
KpnI G_GTAC'C
Cuts at: 0 857 858
Size: 857 1
MscI TGG'CCA
Cuts at: 0 7 858
Size: 7 851
MspA1I CmG'CkG
Cuts at: 0 141 244 345 567 708 792 836 858
Size: 141 103 101 222 141 84 44 22
Fragments arranged by size:
222 141 141 103 101 84 44 22
NcoI C'CATG_G
Cuts at: 0 483 858
Size: 483 375
PasI CC'CwG_GG
Cuts at: 0 229 858
Size: 229 629
PflMI CCAn_nnn'nTGG
Cuts at: 0 411 858
Size: 411 447
PpuMI rG'GwC_Cy
Cuts at: 0 453 858
Size: 453 405
PstI C_TGCA'G
Cuts at: 0 617 704 713 858
Size: 617 87 9 145
Fragments arranged by size:
617 145 87 9
PvuII CAG'CTG
Cuts at: 0 708 858
Size: 708 150
RsrII CG'GwC_CG
Cuts at: 0 779 858
Size: 779 79
SacII CC_GC'GG
Cuts at: 0 142 858
Size: 142 716
SanDI GG'GwC_CC
Cuts at: 0 453 858
Size: 453 405
SbfI CC_TGCA'GG
Cuts at: 0 617 858
Size: 617 241
SfcI C'TryA_G
Cuts at: 0 613 700 709 858
Size: 613 87 9 149
Fragments arranged by size:
613 149 87 9
SmaI CCC'GGG
Cuts at: 0 80 376 858
Size: 80 296 482
Fragments arranged by size:
482 296 80
StyI C'CwwG_G
Cuts at: 0 483 858
Size: 483 375
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 445 512 796 858
Size: 445 67 284 62
Fragments arranged by size:
445 284 67 62
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 487 519 858
Size: 487 32 339
Fragments arranged by size:
487 339 32
XmaI C'CCGG_G
Cuts at: 0 78 374 858
Size: 78 296 484
Fragments arranged by size:
484 296 78
XmnI GAAnn'nnTTC
Cuts at: 0 574 858
Size: 574 284
Enzymes that do cut:
Acc65I AlwNI ApaLI AvaI BanI BanII BcgI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BsaBI
BsaWI BseRI BseYI BsiHKAI Bsp1286I BspEI BspHI BsrFI
BstAPI BstYI BtgI EaeI EarI EciI Eco57MI EcoNI
EcoO109I Hin4I KpnI MscI MspA1I NcoI PasI PflMI
PpuMI PstI PvuII RsrII SacII SanDI SbfI SfcI
SmaI StyI TaqII TstI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbeI BbsI BbvCI
BciVI BclI BfrBI BmgBI BmtI BpuEI BsaI BsaAI
BsaHI BsaXI BsgI BsiEI BsiWI BsmI BsmBI BspMI
BsrBI BsrDI BsrGI BssHII BssSI BstBI BstEII BstXI
BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EagI EcoICRI EcoRI EcoRV FalI FseI FspI
FspAI HaeII HincII HindIII HpaI KasI MfeI MluI
MmeI MslI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PciI PfoI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PvuI SacI
SalI SapI ScaI SexAI SfiI SfoI SgfI SgrAI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
TatI Tth111I XbaI XcmI XhoI ZraI