################################## # NO CUT ENZYMES ( 149 enzymes) # ################################## AatII AccI AccII AccIII AclI AcyI AflII AgeI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI AspI AviII AvrII BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiWI BsmBI BsmFI BsmI Bsp1407I BspDI BspEI BspHI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT22I FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HpaI KasI KspI MamI MfeI MluI MroI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### Acc65I 425 GGTACC G^GTACC AflIII 758 ACATGT A^C[GA][CT]GT AhdI 631 GACATGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Asp700I 1204 GAAACAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 425 GGTACC G^GTACC AspEI 631 GACATGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 934 GAGCTC G[AT]GC[AT]^C AvaI 557 CCCGAG C^[CT]CG[GA]G BalI 968 TGGCCA TGG^CCA BanI 425 GGTACC G^G[CT][GA]CC BsiEI 54 CGACCG CG[GA][CT]^CG BsiHKAI 934 GAGCTC G[AT]GC[AT]^C BsoBI 557 CCCGAG C^[CT]CG[GA]G BspLU11I 758 ACATGT A^CATGT BspMI 545 ACCTGC ACCTGC(4/8) BsrBI 813 GAGCGG CCGCTC(-3/-3) BsrDI 437 CATTGC GCAATG(2/0) BstYI 160 GGATCT [GA]^GATC[CT] EaeI 968 TGGCCA [CT]^GGCC[GA] Eam1105I 631 GACATGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRI 231 GAATTC G^AATTC HphI 810 GGTGA GGTGA(8/7) KpnI 425 GGTACC GGTAC^C MaeII 1079 ACGT A^CGT MaeIII 98 GTGAC ^GT[ACGT]AC MflI 160 GGATCT [GA]^GATC[CT] MluNI 968 TGGCCA TGG^CCA MscI 968 TGGCCA TGG^CCA MslI 857 CATTAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 454 CAGCTG C[AC]G^C[GT]G NheI 877 GCTAGC G^CTAGC NlaIV 425 GGTACC GG[ACGT]^[ACGT]CC PshAI 395 GACTGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 188 CTGCAG CTGCA^G PvuII 454 CAGCTG CAG^CTG SacI 934 GAGCTC GAGCT^C StuI 62 AGGCCT AGG^CCT Tsp45I 98 GTGAC ^GT[GC]AC XcmI 1070 CCAAGGCCGACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 160 GGATCT [GA]^GATC[CT] XmnI 1204 GAAACAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AcsI 231 GAATTC [GA]^AATT[CT] 1117 AAATTT AlwI 1005 GATCC GGATC(4/5) 160 GGATC AlwNI 454 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG 997 CAGAATCTG ApoI 231 GAATTC [GA]^AATT[CT] 1117 AAATTT AvaII 264 GGTCC G^G[AT]CC 1134 GGACC BanII 32 GAGCCC G[GA]GC[CT]^C 934 GAGCTC BmyI 32 GAGCCC G[AGT]GC[ACT]^C 934 GAGCTC BsmAI 39 GTCTC GTCTC(1/5) 1055 GTCTC Bsp1286I 32 GAGCCC G[AGT]GC[ACT]^C 934 GAGCTC FauI 462 GCGGG CCCGC(4/6) 815 GCGGG HindIII 380 AAGCTT A^AGCTT 820 AAGCTT MseI 568 TTAA T^TAA 859 TTAA NspI 632 ACATGC [GA]CATG^[CT] 758 ACATGT PflMI 378 CCAAGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 801 CCAGGCTTTGG SfaNI 146 GCATC GCATC(5/9) 338 GCATC SfcI 188 CTGCAG C^T[GA][CT]AG 305 CTACAG Tru9I 568 TTAA T^TAA 859 TTAA TspRI 125 CAGTG CA[GC]TG[ACGT][ACGT]^ 893 CACTG Van91I 378 CCAAGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 801 CCAGGCTTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 49 enzymes) # ######################################### AciI AfaI AluI BbvI BcnI BfaI BsaJI BsiYI BslI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII ItaI Ksp632I MaeI MboI MboII MnlI MspI MvaI MwoI NciI NdeII NlaIII PleI RsaI Sau3AI Sau96I ScrFI StyI TaqI TfiI TseI Tsp509I TthHB8I