(Circular) (Six-base) MAPSORT of: pFN21AE1893.seq Check: 3559 from: 1 to: 5971 pFN21AE1893 5971 bp With 182 enzymes: SgfI * August 16, 2010 14:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3781 278 Size: 53 83 186 936 2245 2468 Fragments arranged by size: 2468 2245 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3314 1937 Size: 1377 4594 Acc65I G'GTAC_C Cuts at: 2430 3292 2430 Size: 862 5109 AclI AA'CG_TT Cuts at: 1563 4098 4471 1563 Size: 2535 373 3063 Fragments arranged by size: 3063 2535 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2234 2777 4034 5418 5686 1632 Size: 602 543 1257 1384 268 1917 Fragments arranged by size: 1917 1384 1257 602 543 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5774 AflIII A'CryG_T Cuts at: 1203 2763 4870 5847 1203 Size: 1560 2107 977 1327 Fragments arranged by size: 2107 1560 1327 977 AgeI A'CCGG_T Cuts at: 4802 4802 Size: 5971 AhdI GACnn_n'nnGTC Cuts at: 2641 4700 2641 Size: 2059 3912 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5971 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2798 2884 1184 Size: 1614 86 4271 Fragments arranged by size: 4271 1614 86 AlwNI CAG_nnn'CTG Cuts at: 2464 3007 3714 5286 2464 Size: 543 707 1572 3149 Fragments arranged by size: 3149 1572 707 543 ApaLI G'TGCA_C Cuts at: 4028 5184 4028 Size: 1156 4815 ApoI r'AATT_y Cuts at: 1493 2236 3122 3280 3506 3658 5767 1493 Size: 743 886 158 226 152 2109 1697 Fragments arranged by size: 2109 1697 886 743 226 158 152 AseI AT'TA_AT Cuts at: 160 1032 4525 160 Size: 872 3493 1606 Fragments arranged by size: 3493 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5971 AvaI C'yCGr_G Cuts at: 1870 1943 2562 3296 1870 Size: 73 619 734 4545 Fragments arranged by size: 4545 734 619 73 BamHI G'GATC_C Cuts at: 3301 3668 3301 Size: 367 5604 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2430 3292 4747 618 Size: 325 217 627 643 862 1455 1842 Fragments arranged by size: 1842 1455 862 643 627 325 217 BanII G_rGCy'C Cuts at: 1391 2041 2943 3290 1391 Size: 650 902 347 4072 Fragments arranged by size: 4072 902 650 347 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5000 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4162 4196 1903 Size: 34 2225 34 3678 Fragments arranged by size: 3678 2225 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3863 5073 3863 Size: 1210 4761 BclI T'GATC_A Cuts at: 1548 1548 Size: 5971 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4582 136 Size: 107 122 71 631 605 337 2573 1525 Fragments arranged by size: 2573 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 5966 5966 Size: 5971 BlpI GC'TnA_GC Cuts at: 3388 3388 Size: 5971 Bme1580I G_kGCm'C Cuts at: 1790 4032 5188 1790 Size: 2242 1156 2573 Fragments arranged by size: 2573 2242 1156 BmgBI CAC'GTC Cuts at: 1318 3085 1318 Size: 1767 4204 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2035 2171 3173 Size: 416 53 83 399 24 618 136 1002 Cuts at: 3173 3702 4660 442 Size: 529 958 1753 Fragments arranged by size: 1753 1002 958 618 529 416 399 136 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2886 1056 Size: 1830 4141 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4631 1239 Size: 3392 2579 Bpu10I CC'TnA_GC Cuts at: 2543 2877 2543 Size: 334 5637 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4096 4961 5259 4096 Size: 865 298 4808 Fragments arranged by size: 4808 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4634 882 Size: 617 3135 2219 Fragments arranged by size: 3135 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3762 493 Size: 711 2558 2702 Fragments arranged by size: 2702 2558 711 BsaBI GATnn'nnATC Cuts at: 5965 5965 Size: 5971 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3778 4160 4795 275 Size: 53 83 186 936 2245 382 635 1451 Fragments arranged by size: 2245 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4404 4802 5076 5223 5634 4404 Size: 398 274 147 411 4741 Fragments arranged by size: 4741 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5746 Fragments arranged by size: 5746 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5971 BseYI C'CCAG_C Cuts at: 5174 5670 5174 Size: 496 5475 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5398 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2062 3421 4182 5210 1540 Size: 275 186 61 1359 761 1028 2301 Fragments arranged by size: 2301 1359 1028 761 275 186 61 BsiHKAI G_wGCw'C Cuts at: 2943 4032 4117 5188 2943 Size: 1089 85 1071 3726 Fragments arranged by size: 3726 1089 1071 85 BsmI GAATG_Cn' Cuts at: 3482 3575 3482 Size: 93 5878 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2041 2943 3290 4032 4117 5188 1391 Size: 399 251 902 347 742 85 1071 2174 Fragments arranged by size: 2174 1071 902 742 399 347 251 85 BspEI T'CCGG_A Cuts at: 5634 5634 Size: 5971 BspHI T'CATG_A Cuts at: 3860 5590 3860 Size: 1730 4241 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2559 3326 844 Size: 1715 767 3489 Fragments arranged by size: 3489 1715 767 BsrBI CCG'CTC Cuts at: 1394 1823 2820 3858 1394 Size: 429 997 1038 3507 Fragments arranged by size: 3507 1038 997 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2441 4466 4640 65 Size: 1183 1193 2025 174 1396 Fragments arranged by size: 2025 1396 1193 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4615 4802 1700 Size: 110 2805 187 2869 Fragments arranged by size: 2869 2805 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5971 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5971 BssSI C'ACGA_G Cuts at: 4031 5043 4031 Size: 1012 4959 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5971 BstBI TT'CG_AA Cuts at: 4775 4775 Size: 5971 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5971 BstYI r'GATC_y Cuts at: 1972 2165 3301 3668 4053 4070 5522 5608 5620 Size: 193 1136 367 385 17 1452 86 12 Cuts at: 5620 5966 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1136 385 367 346 193 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4480 Fragments arranged by size: 4480 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5232 Fragments arranged by size: 5232 683 56 BtsI GCAGTG_nn' Cuts at: 2136 3487 4281 4301 2136 Size: 1351 794 20 3806 Fragments arranged by size: 3806 1351 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5971 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5936 DraI TTT'AAA Cuts at: 3275 3275 Size: 5971 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4978 809 Size: 507 3662 1802 Fragments arranged by size: 3662 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2973 3418 4307 Size: 54 1425 50 275 18 1143 445 889 Cuts at: 4307 5936 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1143 889 445 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3418 1537 Size: 275 1606 4090 Fragments arranged by size: 4090 1606 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2118 2283 2661 3901 1353 Size: 765 165 378 1240 3423 Fragments arranged by size: 3423 1240 765 378 165 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4558 4932 5078 387 Size: 1064 138 2969 374 146 1280 Fragments arranged by size: 2969 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2941 2941 Size: 5971 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2234 2777 4034 4631 5418 5686 1239 Size: 393 602 543 1257 597 787 268 1524 Fragments arranged by size: 1524 1257 787 602 597 543 393 268 EcoRI G'AATT_C Cuts at: 2236 3280 3658 2236 Size: 1044 378 4549 Fragments arranged by size: 4549 1044 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2531 2531 Size: 5971 FspI TGC'GCA Cuts at: 4477 4477 Size: 5971 HaeII r_GCGC'y Cuts at: 1678 5118 1678 Size: 3440 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2627 2659 4612 4644 4686 Size: 32 135 32 1055 32 1953 32 42 Cuts at: 4686 4718 1373 Size: 32 2626 Fragments arranged by size: 2626 1953 1055 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3315 3561 669 Size: 988 281 1377 246 3079 Fragments arranged by size: 3079 1377 988 281 246 HindIII A'AGCT_T Cuts at: 748 2385 2825 748 Size: 1637 440 3894 Fragments arranged by size: 3894 1637 440 HpaI GTT'AAC Cuts at: 3561 3561 Size: 5971 KpnI G_GTAC'C Cuts at: 2434 3296 2434 Size: 862 5109 MfeI C'AATT_G Cuts at: 3570 3570 Size: 5971 MluI A'CGCG_T Cuts at: 5847 5847 Size: 5971 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5085 5269 1305 Size: 3780 184 2007 Fragments arranged by size: 3780 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2975 10 Size: 54 1425 343 1143 3006 Fragments arranged by size: 3006 1425 1143 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2866 3929 4288 4447 518 Size: 648 1700 1063 359 159 2042 Fragments arranged by size: 2042 1700 1063 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2461 3387 4064 5212 5457 1606 Size: 855 926 677 1148 245 2120 Fragments arranged by size: 2120 1148 926 855 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4480 Fragments arranged by size: 4480 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5971 NheI G'CTAG_C Cuts at: 1052 2882 1052 Size: 1830 4141 NotI GC'GGCC_GC Cuts at: 3418 3418 Size: 5971 NspI r_CATG'y Cuts at: 1132 1229 2641 2767 3329 4874 1132 Size: 97 1412 126 562 1545 2229 Fragments arranged by size: 2229 1545 1412 562 126 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5971 PciI A'CATG_T Cuts at: 2763 4870 2763 Size: 2107 3864 PflMI CCAn_nnn'nTGG Cuts at: 1108 2389 2812 1108 Size: 1281 423 4267 Fragments arranged by size: 4267 1281 423 PfoI T'CCnGG_A Cuts at: 2154 2943 2154 Size: 789 5182 PmeI GTTT'AAAC Cuts at: 3275 3275 Size: 5971 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4040 4072 5553 5585 4040 Size: 32 1481 32 4426 Fragments arranged by size: 4426 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2404 1613 Size: 791 5180 PsiI TTA'TAA Cuts at: 3541 3541 Size: 5971 PstI C_TGCA'G Cuts at: 830 2197 3323 830 Size: 1367 1126 3478 Fragments arranged by size: 3478 1367 1126 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5971 PvuII CAG'CTG Cuts at: 2461 2461 Size: 5971 SacI G_AGCT'C Cuts at: 2943 2943 Size: 5971 SalI G'TCGA_C Cuts at: 1936 3313 1936 Size: 1377 4594 SbfI CC_TGCA'GG Cuts at: 3323 3323 Size: 5971 ScaI AGT'ACT Cuts at: 4219 5931 4219 Size: 1712 4259 SfcI C'TryA_G Cuts at: 826 1045 2193 2310 3319 4454 5135 5326 826 Size: 219 1148 117 1009 1135 681 191 1471 Fragments arranged by size: 1471 1148 1135 1009 681 219 191 117 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5971 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5971 SmaI CCC'GGG Cuts at: 1872 3298 1872 Size: 1426 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4075 4976 5238 820 Size: 197 926 2132 901 262 1553 Fragments arranged by size: 2132 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3762 493 Size: 3269 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5971 SphI G_CATG'C Cuts at: 1132 3329 1132 Size: 2197 3774 SspI AAT'ATT Cuts at: 5 52 3895 5 Size: 47 3843 2081 Fragments arranged by size: 3843 2081 47 StuI AGG'CCT Cuts at: 1517 2069 1517 Size: 552 5419 StyI C'CwwG_G Cuts at: 513 1065 2004 2631 2739 3075 3410 513 Size: 552 939 627 108 336 335 3074 Fragments arranged by size: 3074 939 627 552 336 335 108 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3998 4015 4168 4353 4798 325 Size: 1255 2418 17 153 185 445 1498 Fragments arranged by size: 2418 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2412 2509 2760 Size: 274 80 33 51 1443 435 97 251 Cuts at: 2760 3037 4217 5929 96 Size: 277 1180 1712 138 Fragments arranged by size: 1712 1443 1180 435 277 274 251 138 97 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5931 5963 5931 Size: 32 5939 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5971 XbaI T'CTAG_A Cuts at: 3307 3307 Size: 5971 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3082 3082 Size: 5971 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5971 XmaI C'CCGG_G Cuts at: 1870 3296 1870 Size: 1426 4545 XmnI GAAnn'nnTTC Cuts at: 3213 4100 3213 Size: 887 5084 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3779 276 Size: 53 83 186 936 2245 2468 Fragments arranged by size: 2468 2245 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI