Restriction map for
pFN21AE2551
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# NO CUT ENZYMES ( 111 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AhdI Alw44I AocI Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BbeI BbrPI BbsI BbvCI BciVI
BfrI BglI BlnI BpuAI BseAI
BsiEI BsiWI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstZ17I Bsu36I
ClaI CpoI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoRV EcoT14I FseI FspI
HincII HindII HindIII HpaI KasI
KpnI KspI MluI MroI NaeI
NarI NcoI NgoMIV NotI NruI
NspV PacI PaeR7I PflFI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI Tth111I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AflIII 1365 ACACGT A^C[GA][CT]GT
AgeI 719 ACCGGT A^CCGGT
ApaI 1492 GGGCCC GGGCC^C
AvaI 1596 CCCGGG C^[CT]CG[GA]G
BamHI 1426 GGATCC G^GATCC
BanI 895 GGTGCC G^G[CT][GA]CC
BcgI 430 CGAAATGGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 995 AGATCT A^GATCT
BlpI 214 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 214 GCTGAGC GC^T[ACGT]AGC
BsaBI 1287 GATGTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 238 GAGACC GGTCTC(1/5)
BsaWI 719 ACCGGT [AT]^CCGG[AT]
BsgI 624 CTGCAC GTGCAG(16/14)
BsmBI 4 GAGACG CGTCTC(1/5)
BsoBI 1596 CCCGGG C^[CT]CG[GA]G
BsrFI 719 ACCGGT [GA]^CCGG[CT]
CelII 214 GCTGAGC GC^T[ACGT]AGC
Cfr10I 719 ACCGGT [GA]^CCGG[CT]
DraI 1810 TTTAAA TTT^AAA
EcoRI 18 GAATTC G^AATTC
EcoT22I 438 ATGCAT ATGCA^T
MamI 1287 GATGTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 945 CAATTG C^AATTG
MunI 945 CAATTG C^AATTG
NdeI 321 CATATG CA^TATG
NheI 1042 GCTAGC G^CTAGC
NsiI 438 ATGCAT ATGCA^T
NspI 1247 ACATGC [GA]CATG^[CT]
PinAI 719 ACCGGT A^CCGGT
PleI 115 GAGTC GAGTC(4/5)
PshAI 1198 GACGCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 406 GAGCTC GAGCT^C
SmaI 1596 CCCGGG CCC^GGG
TaqI 1520 TCGA T^CGA
TfiI 386 GAATC G^A[AT]TC
TthHB8I 1520 TCGA T^CGA
XcmI 200 CCACCACCCTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1596 CCCGGG C^CCGGG
XmaI 1596 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccII 698 CGCG CG^CG
1512 CGCG
AcyI 9 GACGCC G[GA]^CG[CT]C
817 GGCGTC
AfaI 138 GTAC GT^AC
423 GTAC
BclI 1337 TGATCA T^GATCA
1790 TGATCA
BfaI 208 CTAG C^TAG
1043 CTAG
BsaAI 1224 CACGTA [CT]AC^GT[GA]
1459 TACGTG
BsaHI 9 GACGCC G[GA]^CG[CT]C
817 GGCGTC
BsmI 1881 GAATGC GAATGC(1/-1)
346 GCATTC
BspMI 1414 ACCTGC ACCTGC(4/8)
1201 GCAGGT
BstEII 1086 GGTGACC G^GT[ACGT]ACC
1434 GGTCACC
BstPI 1086 GGTGACC G^GT[ACGT]ACC
1434 GGTCACC
BstUI 698 CGCG CG^CG
1512 CGCG
DsaI 1403 CCGTGG C^C[GA][CT]GG
1487 CCACGG
EarI 928 CTCTTC CTCTTC(1/4)
1216 CTCTTC
EcoO65I 1086 GGTGACC G^GT[ACGT]ACC
1434 GGTCACC
FbaI 1337 TGATCA T^GATCA
1790 TGATCA
Hin1I 9 GACGCC G[GA]^CG[CT]C
817 GGCGTC
HinfI 115 GAGTC G^A[ACGT]TC
386 GAATC
Ksp632I 928 CTCTTC CTCTTC(1/4)
1216 CTCTTC
MaeI 208 CTAG C^TAG
1043 CTAG
MvnI 698 CGCG CG^CG
1512 CGCG
PflMI 151 CCAAGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
735 CCAGGACCTGG
PstI 709 CTGCAG CTGCA^G
1563 CTGCAG
RsaI 138 GTAC GT^AC
423 GTAC
Van91I 151 CCAAGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
735 CCAGGACCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AciI AcsI AluI AlwI AlwNI
ApoI AspHI AvaII BalI BanII
BbvI BcnI BmyI BpmI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrBI BsrDI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EaeI EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HpaII HphI
ItaI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PpuMI
PvuII Sau3AI Sau96I ScrFI SfaNI
SfcI Tru9I TseI Tsp45I Tsp509I
TspRI XhoII