Restriction map for pFN21AE2551
 (Linear) (Six-base) MAPSORT of: KIEE2551.seq  Check: 3729  from: 1  to: 1890



KIEE2551.orf

 With 182 enzymes: SgfI * 

                        December 2, 2014 13:35  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1423    1890 
   Size:       1423     467
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     704     786    1575    1890 
   Size:        704      82     789     315

  Fragments arranged by size: 

                 789     704     315      82
 
AflIII A'CryG_T

Cuts at:      0    1365    1890 
   Size:       1365     525
 
AgeI A'CCGG_T

Cuts at:      0     719    1890 
   Size:        719    1171
 
AleI CACnn'nnGTG

Cuts at:      0     208    1345    1890 
   Size:        208    1137     545

  Fragments arranged by size: 

                1137     545     208
 
AlwNI CAG_nnn'CTG

Cuts at:      0     741     809    1531    1890 
   Size:        741      68     722     359

  Fragments arranged by size: 

                 741     722     359      68
 
ApaI G_GGCC'C

Cuts at:      0    1496    1890 
   Size:       1496     394
 
ApoI r'AATT_y

Cuts at:      0      18     688    1856    1890 
   Size:         18     670    1168      34

  Fragments arranged by size: 

                1168     670      34      18
 
AvaI C'yCGr_G

Cuts at:      0    1596    1890 
   Size:       1596     294
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1704    1737    1890 
   Size:       1704      33     153

  Fragments arranged by size: 

                1704     153      33
 
BamHI G'GATC_C

Cuts at:      0    1426    1890 
   Size:       1426     464
 
BanI G'GyrC_C

Cuts at:      0     895    1890 
   Size:        895     995
 
BanII G_rGCy'C

Cuts at:      0     410     734    1496    1678    1890 
   Size:        410     324     762     182     212

  Fragments arranged by size: 

                 762     410     324     212     182
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     419     453    1890 
   Size:        419      34    1437

  Fragments arranged by size: 

                1437     419      34
 
BclI T'GATC_A

Cuts at:      0    1337    1790    1890 
   Size:       1337     453     100

  Fragments arranged by size: 

                1337     453     100
 
BfrBI ATG'CAT

Cuts at:      0     440    1890 
   Size:        440    1450
 
BglII A'GATC_T

Cuts at:      0     995    1890 
   Size:        995     895
 
BlpI GC'TnA_GC

Cuts at:      0     215    1890 
   Size:        215    1675
 
Bme1580I G_kGCm'C

Cuts at:      0    1496    1890 
   Size:       1496     394
 
BmgBI CAC'GTC

Cuts at:      0     674     705    1368    1572    1890 
   Size:        674      31     663     204     318

  Fragments arranged by size: 

                 674     663     318     204      31
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     424    1890 
   Size:        424    1466
 
BmtI G_CTAG'C

Cuts at:      0    1046    1890 
   Size:       1046     844
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     718     929     936     982    1495    1629    1737    1784 
   Size:        718     211       7      46     513     134     108      47

Cuts at:   1784    1890 
   Size:        106

  Fragments arranged by size: 

                 718     513     211     134     108     106      47      46
                   7
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     232    1890 
   Size:        232    1658
 
BsaAI yAC'GTr

Cuts at:      0    1226    1461    1890 
   Size:       1226     235     429

  Fragments arranged by size: 

                1226     429     235
 
BsaBI GATnn'nnATC

Cuts at:      0    1291    1890 
   Size:       1291     599
 
BsaHI Gr'CG_yC

Cuts at:      0      10     818    1890 
   Size:         10     808    1072

  Fragments arranged by size: 

                1072     808      10
 
BsaWI w'CCGG_w

Cuts at:      0     719    1890 
   Size:        719    1171
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     547     577    1890 
   Size:        547      30    1313

  Fragments arranged by size: 

                1313     547      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     343     555    1204    1492    1890 
   Size:        343     212     649     288     398

  Fragments arranged by size: 

                 649     398     343     288     212
 
BseYI C'CCAG_C

Cuts at:      0     189    1207    1299    1619    1890 
   Size:        189    1018      92     320     271

  Fragments arranged by size: 

                1018     320     271     189      92
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     609    1890 
   Size:        609    1281
 
BsiHKAI G_wGCw'C

Cuts at:      0     410    1148    1400    1505    1890 
   Size:        410     738     252     105     385

  Fragments arranged by size: 

                 738     410     385     252     105
 
BsmI GAATG_Cn'

Cuts at:      0     346    1887    1890 
   Size:        346    1541       3

  Fragments arranged by size: 

                1541     346       3
 
Bsp1286I G_dGCh'C

Cuts at:      0     410     734    1148    1400    1496    1505    1678    1890 
   Size:        410     324     414     252      96       9     173     212

  Fragments arranged by size: 

                 414     410     324     252     212     173      96       9
            
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1192    1423    1890 
   Size:       1192     231     467

  Fragments arranged by size: 

                1192     467     231
 
BsrBI CCG'CTC

Cuts at:      0     393     991    1389    1890 
   Size:        393     598     398     501

  Fragments arranged by size: 

                 598     501     398     393
 
BsrDI GCAATG_nn'

Cuts at:      0     306     500     512    1890 
   Size:        306     194      12    1378

  Fragments arranged by size: 

                1378     306     194      12
 
BsrFI r'CCGG_y

Cuts at:      0     719    1890 
   Size:        719    1171
 
BstEII G'GTnAC_C

Cuts at:      0    1086    1434    1890 
   Size:       1086     348     456

  Fragments arranged by size: 

                1086     456     348
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     210     433    1370    1890 
   Size:        210     223     937     520

  Fragments arranged by size: 

                 937     520     223     210
 
BstYI r'GATC_y

Cuts at:      0     713     995    1426    1613    1890 
   Size:        713     282     431     187     277

  Fragments arranged by size: 

                 713     431     282     277     187
 
BtgI C'CryG_G

Cuts at:      0    1403    1487    1890 
   Size:       1403      84     403

  Fragments arranged by size: 

                1403     403      84
 
BtsI GCAGTG_nn'

Cuts at:      0    1505    1890 
   Size:       1505     385
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1248    1283    1890 
   Size:       1248      35     607

  Fragments arranged by size: 

                1248     607      35
 
DraI TTT'AAA

Cuts at:      0    1812    1890 
   Size:       1812      78
 
EaeI y'GGCC_r

Cuts at:      0     371    1190    1625    1890 
   Size:        371     819     435     265

  Fragments arranged by size: 

                 819     435     371     265
 
EarI CTCTTCn'nnn_

Cuts at:      0     934    1222    1890 
   Size:        934     288     668

  Fragments arranged by size: 

                 934     668     288
 
EcoICRI GAG'CTC

Cuts at:      0     408    1890 
   Size:        408    1482
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     704     718     786     929     936     982    1495    1575 
   Size:        704      14      68     143       7      46     513      80

Cuts at:   1575    1629    1737    1784    1890 
   Size:         54     108      47     106

  Fragments arranged by size: 

                 704     513     143     108     106      80      68      54
                  47      46      14       7
 
EcoO109I rG'GnC_Cy

Cuts at:      0     738    1026    1204    1454    1492    1890 
   Size:        738     288     178     250      38     398

  Fragments arranged by size: 

                 738     398     288     250     178      38
 
EcoRI G'AATT_C

Cuts at:      0      18    1890 
   Size:         18    1872
 
HaeII r_GCGC'y

Cuts at:      0     312     550    1383    1890 
   Size:        312     238     833     507

  Fragments arranged by size: 

                 833     507     312     238
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1118    1150    1418    1450    1782    1814    1890 
   Size:       1118      32     268      32     332      32      76

  Fragments arranged by size: 

                1118     332     268      76      32      32      32
 
MfeI C'AATT_G

Cuts at:      0     945    1890 
   Size:        945     945
 
MscI TGG'CCA

Cuts at:      0     373    1192    1627    1890 
   Size:        373     819     435     263

  Fragments arranged by size: 

                 819     435     373     263
 
MslI CAynn'nnrTG

Cuts at:      0     208     367     431     535    1345    1890 
   Size:        208     159      64     104     810     545

  Fragments arranged by size: 

                 810     545     208     159     104      64
 
MspA1I CmG'CkG

Cuts at:      0     582     596     755     762    1890 
   Size:        582      14     159       7    1128

  Fragments arranged by size: 

                1128     582     159      14       7
 
NdeI CA'TA_TG

Cuts at:      0     322    1890 
   Size:        322    1568
 
NheI G'CTAG_C

Cuts at:      0    1042    1890 
   Size:       1042     848
 
NsiI A_TGCA'T

Cuts at:      0     442    1890 
   Size:        442    1448
 
NspI r_CATG'y

Cuts at:      0    1251    1890 
   Size:       1251     639
 
PasI CC'CwG_GG

Cuts at:      0     100    1890 
   Size:        100    1790
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     157     741    1890 
   Size:        157     584    1149

  Fragments arranged by size: 

                1149     584     157
 
PfoI T'CCnGG_A

Cuts at:      0     607     734     959    1890 
   Size:        607     127     225     931

  Fragments arranged by size: 

                 931     607     225     127
 
PpuMI rG'GwC_Cy

Cuts at:      0     738    1026    1204    1890 
   Size:        738     288     178     686

  Fragments arranged by size: 

                 738     686     288     178
 
PshAI GACnn'nnGTC

Cuts at:      0    1202    1890 
   Size:       1202     688
 
PspOMI G'GGCC_C

Cuts at:      0    1492    1890 
   Size:       1492     398
 
PstI C_TGCA'G

Cuts at:      0     713    1567    1890 
   Size:        713     854     323

  Fragments arranged by size: 

                 854     713     323
 
PvuII CAG'CTG

Cuts at:      0     582     596     755     762    1890 
   Size:        582      14     159       7    1128

  Fragments arranged by size: 

                1128     582     159      14       7
 
SacI G_AGCT'C

Cuts at:      0     410    1890 
   Size:        410    1480
 
SfcI C'TryA_G

Cuts at:      0      79     709    1563    1890 
   Size:         79     630     854     327

  Fragments arranged by size: 

                 854     630     327      79
 
SmaI CCC'GGG

Cuts at:      0    1598    1890 
   Size:       1598     292
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     834    1611    1890 
   Size:        834     777     279

  Fragments arranged by size: 

                 834     777     279
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     207    1890 
   Size:        207    1683
 
XmaI C'CCGG_G

Cuts at:      0    1596    1890 
   Size:       1596     294
 

 Enzymes that do cut:

     AarI     AcuI   AflIII     AgeI     AleI    AlwNI     ApaI     ApoI
     AvaI     BaeI    BamHI     BanI    BanII     BcgI     BclI    BfrBI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BpmI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
  BsiHKAI     BsmI Bsp1286I    BspMI    BsrBI    BsrDI    BsrFI   BstEII
    BstXI    BstYI     BtgI     BtsI    CspCI     DraI     EaeI     EarI
  EcoICRI  Eco57MI EcoO109I    EcoRI    HaeII    Hin4I     MfeI     MscI
     MslI   MspA1I     NdeI     NheI     NsiI     NspI     PasI    PflMI
     PfoI    PpuMI    PshAI   PspOMI     PstI    PvuII     SacI     SfcI
     SmaI    TaqII     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII     AhdI     AlfI
     AloI    ApaLI     AscI     AseI    AsiSI    AvrII     BbeI     BbsI
    BbvCI    BciVI     BglI     BplI   Bpu10I    BpuEI    BsiEI    BsiWI
    BsmBI    BspEI    BspHI    BsrGI   BssHII    BssSI   BstAPI    BstBI
  BstZ17I   Bsu36I    BtgZI     ClaI   DraIII     DrdI     EagI     EciI
    EcoNI    EcoRV     FalI     FseI     FspI    FspAI   HincII  HindIII
     HpaI     KasI     KpnI     MluI     MmeI     NaeI     NarI     NcoI
   NgoMIV     NotI     NruI     PacI     PciI     PmeI     PmlI     PpiI
     PsiI    PspXI     PsrI     PvuI    RsrII    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI     TatI     TstI  Tth111I     XbaI     XhoI     XmnI     ZraI
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