(Circular) (Six-base) MAPSORT of: pFN21AB4192.seq Check: 2845 from: 1 to: 6184 pFN21AB4192 6184 bp With 182 enzymes: SgfI * August 20, 2012 11:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2694 3994 278 Size: 53 83 186 936 1158 1300 2468 Fragments arranged by size: 2468 1300 1158 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2394 2550 3527 1937 Size: 457 156 977 4594 Fragments arranged by size: 4594 977 457 156 Acc65I G'GTAC_C Cuts at: 3505 3505 Size: 6184 AclI AA'CG_TT Cuts at: 1563 4311 4684 1563 Size: 2748 373 3063 Fragments arranged by size: 3063 2748 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2292 3385 4247 5631 5899 1632 Size: 660 1093 862 1384 268 1917 Fragments arranged by size: 1917 1384 1093 862 660 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5987 AflIII A'CryG_T Cuts at: 1203 2985 5083 6060 1203 Size: 1782 2098 977 1327 Fragments arranged by size: 2098 1782 1327 977 AgeI A'CCGG_T Cuts at: 5015 5015 Size: 6184 AhdI GACnn_n'nnGTC Cuts at: 4913 4913 Size: 6184 AleI CACnn'nnGTG Cuts at: 1166 2658 1166 Size: 1492 4692 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6184 AlwNI CAG_nnn'CTG Cuts at: 2445 3241 3927 5499 2445 Size: 796 686 1572 3130 Fragments arranged by size: 3130 1572 796 686 ApaLI G'TGCA_C Cuts at: 4241 5397 4241 Size: 1156 5028 ApoI r'AATT_y Cuts at: 1493 3493 3719 3871 5980 1493 Size: 2000 226 152 2109 1697 Fragments arranged by size: 2109 2000 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4738 160 Size: 872 3706 1606 Fragments arranged by size: 3706 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6184 AvaI C'yCGr_G Cuts at: 1870 1943 2635 2767 3083 3093 3257 3428 3509 Size: 73 692 132 316 10 164 171 81 Cuts at: 3509 1870 Size: 4545 Fragments arranged by size: 4545 692 316 171 164 132 81 73 10 BamHI G'GATC_C Cuts at: 3514 3881 3514 Size: 367 5817 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2663 3201 3245 3505 4960 Size: 325 217 627 876 538 44 260 1455 Cuts at: 4960 618 Size: 1842 Fragments arranged by size: 1842 1455 876 627 538 325 260 217 44 BanII G_rGCy'C Cuts at: 1391 2017 3071 3183 3413 3503 1391 Size: 626 1054 112 230 90 4072 Fragments arranged by size: 4072 1054 626 230 112 90 BbeI G_GCGC'C Cuts at: 2667 3249 2667 Size: 582 5602 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2851 928 Size: 971 952 4261 Fragments arranged by size: 4261 971 952 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4375 4409 1903 Size: 34 2438 34 3678 Fragments arranged by size: 3678 2438 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2137 3485 4076 5286 2137 Size: 1348 591 1210 3035 Fragments arranged by size: 3035 1348 1210 591 BclI T'GATC_A Cuts at: 1548 1548 Size: 6184 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3066 3095 3112 Size: 107 122 71 631 605 1394 29 17 Cuts at: 3112 4795 136 Size: 1683 1525 Fragments arranged by size: 1683 1525 1394 631 605 122 107 71 29 17 BglII A'GATC_T Cuts at: 6179 6179 Size: 6184 BlpI GC'TnA_GC Cuts at: 3601 3601 Size: 6184 Bme1580I G_kGCm'C Cuts at: 1790 2754 4245 5401 1790 Size: 964 1491 1156 2573 Fragments arranged by size: 2573 1491 1156 964 BmgBI CAC'GTC Cuts at: 1318 2415 1318 Size: 1097 5087 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3352 3915 4873 Size: 416 53 83 399 24 1935 563 958 Cuts at: 4873 442 Size: 1753 Fragments arranged by size: 1935 1753 958 563 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6184 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2422 2422 Size: 6184 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3065 3397 4844 1239 Size: 1826 332 1447 2579 Fragments arranged by size: 2579 1826 1447 332 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2147 4309 5174 5472 2147 Size: 2162 865 298 2859 Fragments arranged by size: 2859 2162 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2833 4847 882 Size: 617 1334 2014 2219 Fragments arranged by size: 2219 2014 1334 617 BsaAI yAC'GTr Cuts at: 493 1204 3975 493 Size: 711 2771 2702 Fragments arranged by size: 2771 2702 711 BsaBI GATnn'nnATC Cuts at: 2234 2582 6178 2234 Size: 348 3596 2240 Fragments arranged by size: 3596 2240 348 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2379 2664 2691 2999 Size: 53 83 186 936 846 285 27 308 Cuts at: 2999 3119 3246 3991 4373 5008 275 Size: 120 127 745 382 635 1451 Fragments arranged by size: 1451 936 846 745 635 382 308 285 186 127 120 83 53 27 BsaWI w'CCGG_w Cuts at: 4617 5015 5289 5436 5847 4617 Size: 398 274 147 411 4954 Fragments arranged by size: 4954 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5959 Fragments arranged by size: 5959 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2024 2219 3164 1186 Size: 838 195 945 4206 Fragments arranged by size: 4206 945 838 195 BseYI C'CCAG_C Cuts at: 2991 5387 5883 2991 Size: 2396 496 3292 Fragments arranged by size: 3292 2396 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5611 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3634 4395 5423 1540 Size: 275 186 1633 761 1028 2301 Fragments arranged by size: 2301 1633 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2391 2763 2982 3183 3305 3399 4245 4330 5401 Size: 372 219 201 122 94 846 85 1071 Cuts at: 5401 2391 Size: 3174 Fragments arranged by size: 3174 1071 846 372 219 201 122 94 85 BsmI GAATG_Cn' Cuts at: 3364 3695 3788 3364 Size: 331 93 5760 Fragments arranged by size: 5760 331 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2017 2391 2754 2763 2982 3071 3183 Size: 399 227 374 363 9 219 89 112 Cuts at: 3183 3305 3399 3413 3503 4245 4330 5401 1391 Size: 122 94 14 90 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 374 363 227 219 122 112 94 90 89 85 14 9 BspEI T'CCGG_A Cuts at: 5847 5847 Size: 6184 BspHI T'CATG_A Cuts at: 2961 4073 5803 2961 Size: 1112 1730 3342 Fragments arranged by size: 3342 1730 1112 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2910 3539 844 Size: 2066 629 3489 Fragments arranged by size: 3489 2066 629 BsrBI CCG'CTC Cuts at: 1394 1823 2505 2725 2911 3389 4071 1394 Size: 429 682 220 186 478 682 3507 Fragments arranged by size: 3507 682 682 478 429 220 186 BsrDI GCAATG_nn' Cuts at: 65 1248 2871 4679 4853 65 Size: 1183 1623 1808 174 1396 Fragments arranged by size: 1808 1623 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2121 2797 2807 3204 3323 4828 5015 Size: 110 311 676 10 397 119 1505 187 Cuts at: 5015 1700 Size: 2869 Fragments arranged by size: 2869 1505 676 397 311 187 119 110 10 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6184 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6184 BssSI C'ACGA_G Cuts at: 2315 3182 3304 4244 5256 2315 Size: 867 122 940 1012 3243 Fragments arranged by size: 3243 1012 940 867 122 BstAPI GCAn_nnn'nTGC Cuts at: 794 2445 794 Size: 1651 4533 BstBI TT'CG_AA Cuts at: 4988 4988 Size: 6184 BstEII G'GTnAC_C Cuts at: 1184 2167 2458 1184 Size: 983 291 4910 Fragments arranged by size: 4910 983 291 BstXI CCAn_nnnn'nTGG Cuts at: 2660 2660 Size: 6184 BstYI r'GATC_y Cuts at: 1972 3514 3881 4266 4283 5735 5821 5833 6179 Size: 1542 367 385 17 1452 86 12 346 Cuts at: 6179 1972 Size: 1977 Fragments arranged by size: 1977 1542 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2139 2139 Size: 6184 BtgI C'CryG_G Cuts at: 513 1065 2004 2656 3023 3170 513 Size: 552 939 652 367 147 3527 Fragments arranged by size: 3527 939 652 552 367 147 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5445 Fragments arranged by size: 5445 683 56 BtsI GCAGTG_nn' Cuts at: 2457 3700 4494 4514 2457 Size: 1243 794 20 4127 Fragments arranged by size: 4127 1243 794 20 ClaI AT'CG_AT Cuts at: 1554 2238 1554 Size: 684 5500 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2938 2973 529 Size: 35 2374 35 3740 Fragments arranged by size: 3740 2374 35 35 DraI TTT'AAA Cuts at: 3488 3488 Size: 6184 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5191 809 Size: 507 3875 1802 Fragments arranged by size: 3875 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2123 2356 2427 Size: 54 1425 50 275 18 293 233 71 Cuts at: 2427 2812 3631 4520 6149 8 Size: 385 819 889 1629 43 Fragments arranged by size: 1629 1425 889 819 385 293 275 233 71 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3631 1537 Size: 275 1819 4090 Fragments arranged by size: 4090 1819 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2102 2520 2732 3404 4114 1353 Size: 749 418 212 672 710 3423 Fragments arranged by size: 3423 749 710 672 418 212 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2636 2702 4771 5145 5291 387 Size: 1064 138 1047 66 2069 374 146 1280 Fragments arranged by size: 2069 1280 1064 1047 374 146 138 66 EcoICRI GAG'CTC Cuts at: 3181 3181 Size: 6184 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2292 3065 3385 3397 4247 4844 5631 Size: 393 660 773 320 12 850 597 787 Cuts at: 5631 5899 1239 Size: 268 1524 Fragments arranged by size: 1524 850 787 773 660 597 393 320 268 12 EcoO109I rG'GnC_Cy Cuts at: 2026 3088 3213 3432 2026 Size: 1062 125 219 4778 Fragments arranged by size: 4778 1062 219 125 EcoRI G'AATT_C Cuts at: 3493 3871 3493 Size: 378 5806 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2293 2743 2293 Size: 450 5734 FspI TGC'GCA Cuts at: 2449 3350 4690 2449 Size: 901 1340 3943 Fragments arranged by size: 3943 1340 901 FspAI rTGC'GCAy Cuts at: 3350 3350 Size: 6184 HaeII r_GCGC'y Cuts at: 1678 2667 3249 5331 1678 Size: 989 582 2082 2531 Fragments arranged by size: 2531 2082 989 582 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3320 3352 4825 4857 4899 Size: 32 135 32 1748 32 1473 32 42 Cuts at: 4899 4931 1373 Size: 32 2626 Fragments arranged by size: 2626 1748 1473 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3528 3774 669 Size: 988 281 1590 246 3079 Fragments arranged by size: 3079 1590 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6184 HpaI GTT'AAC Cuts at: 3774 3774 Size: 6184 KasI G'GCGC_C Cuts at: 2663 3245 2663 Size: 582 5602 KpnI G_GTAC'C Cuts at: 3509 3509 Size: 6184 MfeI C'AATT_G Cuts at: 3783 3783 Size: 6184 MluI A'CGCG_T Cuts at: 6060 6060 Size: 6184 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3363 5298 5482 1305 Size: 2058 1935 184 2007 Fragments arranged by size: 2058 2007 1935 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2358 10 Size: 54 1425 343 526 3836 Fragments arranged by size: 3836 1425 526 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2658 2747 2960 4142 4501 4660 518 Size: 648 1492 89 213 1182 359 159 2042 Fragments arranged by size: 2042 1492 1182 648 359 213 159 89 MspA1I CmG'CkG Cuts at: 1606 2995 3025 3267 3600 4277 5425 5670 1606 Size: 1389 30 242 333 677 1148 245 2120 Fragments arranged by size: 2120 1389 1148 677 333 245 242 30 NaeI GCC'GGC Cuts at: 2123 2799 2809 2123 Size: 676 10 5498 Fragments arranged by size: 5498 676 10 NarI GG'CG_CC Cuts at: 2664 3246 2664 Size: 582 5602 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4693 Fragments arranged by size: 4693 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6184 NgoMIV G'CCGG_C Cuts at: 2121 2797 2807 2121 Size: 676 10 5498 Fragments arranged by size: 5498 676 10 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6184 NotI GC'GGCC_GC Cuts at: 3631 3631 Size: 6184 NspI r_CATG'y Cuts at: 1132 1229 3542 5087 1132 Size: 97 2313 1545 2229 Fragments arranged by size: 2313 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2678 1348 Size: 1330 4854 PciI A'CATG_T Cuts at: 5083 5083 Size: 6184 PflMI CCAn_nnn'nTGG Cuts at: 1108 3241 1108 Size: 2133 4051 PfoI T'CCnGG_A Cuts at: 3042 3042 Size: 6184 PmeI GTTT'AAAC Cuts at: 3488 3488 Size: 6184 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2519 2551 4253 4285 5766 5798 2519 Size: 32 1702 32 1481 32 2905 Fragments arranged by size: 2905 1702 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2026 3213 2026 Size: 1187 4997 PshAI GACnn'nnGTC Cuts at: 1613 2690 1613 Size: 1077 5107 PsiI TTA'TAA Cuts at: 3754 3754 Size: 6184 PstI C_TGCA'G Cuts at: 830 3536 830 Size: 2706 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6184 SacI G_AGCT'C Cuts at: 3183 3183 Size: 6184 SacII CC_GC'GG Cuts at: 3026 3026 Size: 6184 SalI G'TCGA_C Cuts at: 1936 3526 1936 Size: 1590 4594 SapI GCTCTTCn'nnn_ Cuts at: 2732 3404 2732 Size: 672 5512 SbfI CC_TGCA'GG Cuts at: 3536 3536 Size: 6184 ScaI AGT'ACT Cuts at: 4432 6144 4432 Size: 1712 4472 SfcI C'TryA_G Cuts at: 826 1045 2263 3532 4667 5348 5539 826 Size: 219 1218 1269 1135 681 191 1471 Fragments arranged by size: 1471 1269 1218 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 3095 3095 Size: 6184 SfoI GGC'GCC Cuts at: 2665 3247 2665 Size: 582 5602 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6184 SgrAI Cr'CCGG_yG Cuts at: 1700 3323 1700 Size: 1623 4561 SmaI CCC'GGG Cuts at: 1872 2637 3095 3511 1872 Size: 765 458 416 4545 Fragments arranged by size: 4545 765 458 416 SmlI C'TyrA_G Cuts at: 820 1017 1943 2162 4288 5189 5451 820 Size: 197 926 219 2126 901 262 1553 Fragments arranged by size: 2126 1553 926 901 262 219 197 SnaBI TAC'GTA Cuts at: 493 3975 493 Size: 3482 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6184 SphI G_CATG'C Cuts at: 1132 3542 1132 Size: 2410 3774 SspI AAT'ATT Cuts at: 5 52 2350 4108 5 Size: 47 2298 1758 2081 Fragments arranged by size: 2298 2081 1758 47 StuI AGG'CCT Cuts at: 1517 2597 1517 Size: 1080 5104 StyI C'CwwG_G Cuts at: 513 1065 2004 3110 3623 513 Size: 552 939 1106 513 3074 Fragments arranged by size: 3074 1106 939 552 513 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3023 3334 4211 4228 4381 4566 5011 Size: 1255 1443 311 877 17 153 185 445 Cuts at: 5011 325 Size: 1498 Fragments arranged by size: 1498 1443 1255 877 445 311 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4430 6142 96 Size: 274 80 33 51 1443 2453 1712 138 Fragments arranged by size: 2453 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3002 3034 6144 6176 3002 Size: 32 3110 32 3010 Fragments arranged by size: 3110 3010 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6184 XbaI T'CTAG_A Cuts at: 3520 3520 Size: 6184 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6184 XmaI C'CCGG_G Cuts at: 1870 2635 3093 3509 1870 Size: 765 458 416 4545 Fragments arranged by size: 4545 765 458 416 XmnI GAAnn'nnTTC Cuts at: 4313 4313 Size: 6184 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2692 3992 276 Size: 53 83 186 936 1158 1300 2468 Fragments arranged by size: 2468 1300 1158 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FseI NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SanDI SexAI SrfI SwaI XcmI