Restriction map for pFN21AB4192
 (Linear) (Six-base) MAPSORT of: KIBB4192.seq  Check: 2304  from: 1  to: 1479



KIBB4192.orf

 With 182 enzymes: SgfI * 

                        January 17, 2012 13:59  ..

AatII G_ACGT'C

Cuts at:      0     689    1479 
   Size:        689     790
 
AccI GT'mk_AC

Cuts at:      0     389     545    1479 
   Size:        389     156     934

  Fragments arranged by size: 

                 934     389     156
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     287    1380    1479 
   Size:        287    1093      99

  Fragments arranged by size: 

                1093     287      99
 
AflIII A'CryG_T

Cuts at:      0     980    1479 
   Size:        980     499
 
AleI CACnn'nnGTG

Cuts at:      0     653    1479 
   Size:        653     826
 
AlwNI CAG_nnn'CTG

Cuts at:      0     440    1236    1479 
   Size:        440     796     243

  Fragments arranged by size: 

                 796     440     243
 
AvaI C'yCGr_G

Cuts at:      0     630     762    1078    1088    1252    1423    1479 
   Size:        630     132     316      10     164     171      56

  Fragments arranged by size: 

                 630     316     171     164     132      56      10
 
BanI G'GyrC_C

Cuts at:      0     658    1196    1240    1479 
   Size:        658     538      44     239

  Fragments arranged by size: 

                 658     538     239      44
 
BanII G_rGCy'C

Cuts at:      0      12    1066    1178    1408    1479 
   Size:         12    1054     112     230      71

  Fragments arranged by size: 

                1054     230     112      71      12
 
BbeI G_GCGC'C

Cuts at:      0     662    1244    1479 
   Size:        662     582     235

  Fragments arranged by size: 

                 662     582     235
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     846    1479 
   Size:        846     633
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     132    1479 
   Size:        132    1347
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1061    1090    1107    1479 
   Size:       1061      29      17     372

  Fragments arranged by size: 

                1061     372      29      17
 
Bme1580I G_kGCm'C

Cuts at:      0     749    1479 
   Size:        749     730
 
BmgBI CAC'GTC

Cuts at:      0     410    1479 
   Size:        410    1069
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1347    1479 
   Size:       1347     132
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     417    1479 
   Size:        417    1062
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1060    1392    1479 
   Size:       1060     332      87

  Fragments arranged by size: 

                1060     332      87
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     142    1479 
   Size:        142    1337
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     828    1479 
   Size:        828     651
 
BsaBI GATnn'nnATC

Cuts at:      0     229     577    1479 
   Size:        229     348     902

  Fragments arranged by size: 

                 902     348     229
 
BsaHI Gr'CG_yC

Cuts at:      0     374     659     686     994    1114    1241    1479 
   Size:        374     285      27     308     120     127     238

  Fragments arranged by size: 

                 374     308     285     238     127     120      27
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      19     214    1159    1479 
   Size:         19     195     945     320

  Fragments arranged by size: 

                 945     320     195      19
 
BseYI C'CCAG_C

Cuts at:      0     986    1479 
   Size:        986     493
 
BsiHKAI G_wGCw'C

Cuts at:      0     386     758     977    1178    1300    1394    1479 
   Size:        386     372     219     201     122      94      85

  Fragments arranged by size: 

                 386     372     219     201     122      94      85
 
BsmI GAATG_Cn'

Cuts at:      0    1359    1479 
   Size:       1359     120
 
Bsp1286I G_dGCh'C

Cuts at:      0      12     386     749     758     977    1066    1178    1300 
   Size:         12     374     363       9     219      89     112     122

Cuts at:   1300    1394    1408    1479 
   Size:         94      14      71

  Fragments arranged by size: 

                 374     363     219     122     112      94      89      71
                  14      12       9
 
BspHI T'CATG_A

Cuts at:      0     956    1479 
   Size:        956     523
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     905    1479 
   Size:        905     574
 
BsrBI CCG'CTC

Cuts at:      0     500     720     906    1384    1479 
   Size:        500     220     186     478      95

  Fragments arranged by size: 

                 500     478     220     186      95
 
BsrDI GCAATG_nn'

Cuts at:      0     866    1479 
   Size:        866     613
 
BsrFI r'CCGG_y

Cuts at:      0     116     792     802    1199    1318    1479 
   Size:        116     676      10     397     119     161

  Fragments arranged by size: 

                 676     397     161     119     116      10
 
BssSI C'ACGA_G

Cuts at:      0     310    1177    1299    1479 
   Size:        310     867     122     180

  Fragments arranged by size: 

                 867     310     180     122
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     440    1479 
   Size:        440    1039
 
BstEII G'GTnAC_C

Cuts at:      0     162     453    1479 
   Size:        162     291    1026

  Fragments arranged by size: 

                1026     291     162
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     655    1479 
   Size:        655     824
 
Bsu36I CC'TnA_GG

Cuts at:      0     134    1479 
   Size:        134    1345
 
BtgI C'CryG_G

Cuts at:      0     651    1018    1165    1479 
   Size:        651     367     147     314

  Fragments arranged by size: 

                 651     367     314     147
 
BtsI GCAGTG_nn'

Cuts at:      0     452    1479 
   Size:        452    1027
 
ClaI AT'CG_AT

Cuts at:      0     233    1479 
   Size:        233    1246
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     933     968    1479 
   Size:        933      35     511

  Fragments arranged by size: 

                 933     511      35
 
EaeI y'GGCC_r

Cuts at:      0     118     351     422     807    1479 
   Size:        118     233      71     385     672

  Fragments arranged by size: 

                 672     385     233     118      71
 
EarI CTCTTCn'nnn_

Cuts at:      0      97     515     727    1399    1479 
   Size:         97     418     212     672      80

  Fragments arranged by size: 

                 672     418     212      97      80
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     631     697    1479 
   Size:        631      66     782

  Fragments arranged by size: 

                 782     631      66
 
EcoICRI GAG'CTC

Cuts at:      0    1176    1479 
   Size:       1176     303
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     287    1060    1380    1392    1479 
   Size:        287     773     320      12      87

  Fragments arranged by size: 

                 773     320     287      87      12
 
EcoO109I rG'GnC_Cy

Cuts at:      0      21    1083    1208    1427    1479 
   Size:         21    1062     125     219      52

  Fragments arranged by size: 

                1062     219     125      52      21
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     288     738    1479 
   Size:        288     450     741

  Fragments arranged by size: 

                 741     450     288
 
FspI TGC'GCA

Cuts at:      0     444    1345    1479 
   Size:        444     901     134

  Fragments arranged by size: 

                 901     444     134
 
FspAI rTGC'GCAy

Cuts at:      0    1345    1479 
   Size:       1345     134
 
HaeII r_GCGC'y

Cuts at:      0     662    1244    1479 
   Size:        662     582     235

  Fragments arranged by size: 

                 662     582     235
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1315    1347    1479 
   Size:       1315      32     132

  Fragments arranged by size: 

                1315     132      32
 
KasI G'GCGC_C

Cuts at:      0     658    1240    1479 
   Size:        658     582     239

  Fragments arranged by size: 

                 658     582     239
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1358    1479 
   Size:       1358     121
 
MscI TGG'CCA

Cuts at:      0     353    1479 
   Size:        353    1126
 
MslI CAynn'nnrTG

Cuts at:      0     653     742     955    1479 
   Size:        653      89     213     524

  Fragments arranged by size: 

                 653     524     213      89
 
MspA1I CmG'CkG

Cuts at:      0     990    1020    1262    1479 
   Size:        990      30     242     217

  Fragments arranged by size: 

                 990     242     217      30
 
NaeI GCC'GGC

Cuts at:      0     118     794     804    1479 
   Size:        118     676      10     675

  Fragments arranged by size: 

                 676     675     118      10
 
NarI GG'CG_CC

Cuts at:      0     659    1241    1479 
   Size:        659     582     238

  Fragments arranged by size: 

                 659     582     238
 
NgoMIV G'CCGG_C

Cuts at:      0     116     792     802    1479 
   Size:        116     676      10     677

  Fragments arranged by size: 

                 677     676     116      10
 
PasI CC'CwG_GG

Cuts at:      0     673    1479 
   Size:        673     806
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1236    1479 
   Size:       1236     243
 
PfoI T'CCnGG_A

Cuts at:      0    1037    1479 
   Size:       1037     442
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     514     546    1479 
   Size:        514      32     933

  Fragments arranged by size: 

                 933     514      32
 
PpuMI rG'GwC_Cy

Cuts at:      0      21    1208    1479 
   Size:         21    1187     271

  Fragments arranged by size: 

                1187     271      21
 
PshAI GACnn'nnGTC

Cuts at:      0     685    1479 
   Size:        685     794
 
SacI G_AGCT'C

Cuts at:      0    1178    1479 
   Size:       1178     301
 
SacII CC_GC'GG

Cuts at:      0    1021    1479 
   Size:       1021     458
 
SapI GCTCTTCn'nnn_

Cuts at:      0     727    1399    1479 
   Size:        727     672      80

  Fragments arranged by size: 

                 727     672      80
 
SfcI C'TryA_G

Cuts at:      0     258    1479 
   Size:        258    1221
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1090    1479 
   Size:       1090     389
 
SfoI GGC'GCC

Cuts at:      0     660    1242    1479 
   Size:        660     582     237

  Fragments arranged by size: 

                 660     582     237
 
SgrAI Cr'CCGG_yG

Cuts at:      0    1318    1479 
   Size:       1318     161
 
SmaI CCC'GGG

Cuts at:      0     632    1090    1479 
   Size:        632     458     389

  Fragments arranged by size: 

                 632     458     389
 
SmlI C'TyrA_G

Cuts at:      0     157    1479 
   Size:        157    1322
 
SspI AAT'ATT

Cuts at:      0     345    1479 
   Size:        345    1134
 
StuI AGG'CCT

Cuts at:      0     592    1479 
   Size:        592     887
 
StyI C'CwwG_G

Cuts at:      0    1105    1479 
   Size:       1105     374
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1018    1329    1479 
   Size:       1018     311     150

  Fragments arranged by size: 

                1018     311     150
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     997    1029    1479 
   Size:        997      32     450

  Fragments arranged by size: 

                 997     450      32
 
XmaI C'CCGG_G

Cuts at:      0     630    1088    1479 
   Size:        630     458     391

  Fragments arranged by size: 

                 630     458     391
 
ZraI GAC'GTC

Cuts at:      0     687    1479 
   Size:        687     792
 

 Enzymes that do cut:

    AatII     AccI     AcuI   AflIII     AleI    AlwNI     AvaI     BanI
    BanII     BbeI     BbsI    BciVI     BglI Bme1580I    BmgBI     BmrI
     BplI     BpmI    BpuEI     BsaI    BsaBI    BsaHI    BseRI    BseYI
  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BssSI   BstAPI   BstEII    BstXI   Bsu36I     BtgI     BtsI     ClaI
    CspCI     EaeI     EarI     EciI  EcoICRI  Eco57MI EcoO109I     FalI
     FspI    FspAI    HaeII    Hin4I     KasI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI   NgoMIV     PasI    PflMI     PfoI     PpiI
    PpuMI    PshAI     SacI    SacII     SapI     SfcI     SfiI     SfoI
    SgrAI     SmaI     SmlI     SspI     StuI     StyI    TaqII     TstI
     XmaI     ZraI

 Enzymes that do not cut: 

     AarI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI     AlfI
     AloI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI    BbvCI     BcgI     BclI    BfrBI    BglII     BlpI
     BmtI   Bpu10I    BsaAI    BsaWI    BsaXI     BsgI    BsiEI    BsiWI
    BsmBI    BspEI    BsrGI   BssHII    BstBI    BstYI  BstZ17I    BtgZI
     DraI   DraIII     DrdI     EagI    EcoNI    EcoRI    EcoRV     FseI
   HincII  HindIII     HpaI     KpnI     MfeI     MluI     NcoI     NdeI
     NheI     NotI     NruI     NsiI     NspI     PacI     PciI     PmeI
     PmlI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SalI    SanDI     SbfI     ScaI    SexAI     SgfI    SnaBI
     SpeI     SphI     SrfI     SwaI     TatI  Tth111I     XbaI     XcmI
     XhoI     XmnI
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