(Circular) (Six-base) MAPSORT of: pFN21AE1913.seq Check: 5362 from: 1 to: 6055 pFN21AE1913 6055 bp With 182 enzymes: SgfI * July 19, 2019 13:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3865 278 Size: 53 83 186 936 2329 2468 Fragments arranged by size: 2468 2329 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2151 3398 1937 Size: 214 1247 4594 Fragments arranged by size: 4594 1247 214 Acc65I G'GTAC_C Cuts at: 3376 3376 Size: 6055 AclI AA'CG_TT Cuts at: 1563 4182 4555 1563 Size: 2619 373 3063 Fragments arranged by size: 3063 2619 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2242 2488 4118 5502 5770 1632 Size: 610 246 1630 1384 268 1917 Fragments arranged by size: 1917 1630 1384 610 268 246 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5858 AflIII A'CryG_T Cuts at: 1203 4954 5931 1203 Size: 3751 977 1327 Fragments arranged by size: 3751 1327 977 AgeI A'CCGG_T Cuts at: 4886 4886 Size: 6055 AhdI GACnn_n'nnGTC Cuts at: 3259 4784 3259 Size: 1525 4530 AleI CACnn'nnGTG Cuts at: 1166 2540 1166 Size: 1374 4681 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6055 AlwNI CAG_nnn'CTG Cuts at: 2995 3133 3798 5370 2995 Size: 138 665 1572 3680 Fragments arranged by size: 3680 1572 665 138 ApaI G_GGCC'C Cuts at: 2854 3350 2854 Size: 496 5559 ApaLI G'TGCA_C Cuts at: 2018 4112 5268 2018 Size: 2094 1156 2805 Fragments arranged by size: 2805 2094 1156 ApoI r'AATT_y Cuts at: 1493 3364 3590 3742 5851 1493 Size: 1871 226 152 2109 1697 Fragments arranged by size: 2109 1871 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4609 160 Size: 872 3577 1606 Fragments arranged by size: 3577 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6055 AvaI C'yCGr_G Cuts at: 1870 1943 2187 2731 2829 2864 3380 1870 Size: 73 244 544 98 35 516 4545 Fragments arranged by size: 4545 544 516 244 98 73 35 BamHI G'GATC_C Cuts at: 3036 3385 3752 3036 Size: 349 367 5339 Fragments arranged by size: 5339 367 349 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2776 3376 4831 618 Size: 325 217 627 989 600 1455 1842 Fragments arranged by size: 1842 1455 989 627 600 325 217 BanII G_rGCy'C Cuts at: 1391 2438 2550 2854 2871 3350 3374 1391 Size: 1047 112 304 17 479 24 4072 Fragments arranged by size: 4072 1047 479 304 112 24 17 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3324 928 Size: 971 1425 3659 Fragments arranged by size: 3659 1425 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2032 2066 3007 3041 4246 4280 1903 Size: 34 95 34 941 34 1205 34 3678 Fragments arranged by size: 3678 1205 941 95 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2490 3947 5157 2490 Size: 1457 1210 3388 Fragments arranged by size: 3388 1457 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6055 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2040 2397 4666 Size: 107 122 71 631 605 368 357 2269 Cuts at: 4666 136 Size: 1525 Fragments arranged by size: 2269 1525 631 605 368 357 122 107 71 BglII A'GATC_T Cuts at: 6050 6050 Size: 6055 BlpI GC'TnA_GC Cuts at: 3472 3472 Size: 6055 Bme1580I G_kGCm'C Cuts at: 1790 2022 2450 2517 2781 2854 2865 3350 4116 Size: 232 428 67 264 73 11 485 766 Cuts at: 4116 5272 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 766 485 428 264 232 73 67 11 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6055 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2314 3786 4744 Size: 416 53 83 399 24 897 1472 958 Cuts at: 4744 442 Size: 1753 Fragments arranged by size: 1753 1472 958 897 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6055 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2156 4715 1239 Size: 917 2559 2579 Fragments arranged by size: 2579 2559 917 Bpu10I CC'TnA_GC Cuts at: 2236 2236 Size: 6055 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2633 3071 4180 5045 5343 2633 Size: 438 1109 865 298 3345 Fragments arranged by size: 3345 1109 865 438 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2591 4718 882 Size: 617 1092 2127 2219 Fragments arranged by size: 2219 2127 1092 617 BsaAI yAC'GTr Cuts at: 493 1204 2113 2182 3846 493 Size: 711 909 69 1664 2702 Fragments arranged by size: 2702 1664 909 711 69 BsaBI GATnn'nnATC Cuts at: 6049 6049 Size: 6055 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3285 3862 4244 4879 Size: 53 83 186 936 1752 577 382 635 Cuts at: 4879 275 Size: 1451 Fragments arranged by size: 1752 1451 936 635 577 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4488 4886 5160 5307 5718 4488 Size: 398 274 147 411 4825 Fragments arranged by size: 4825 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5830 Fragments arranged by size: 5830 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2489 3341 3344 1186 Size: 1303 852 3 3897 Fragments arranged by size: 3897 1303 852 3 BseYI C'CCAG_C Cuts at: 2099 2419 2874 5258 5754 2099 Size: 320 455 2384 496 2400 Fragments arranged by size: 2400 2384 496 455 320 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2777 3173 1163 Size: 573 1041 396 4045 Fragments arranged by size: 4045 1041 573 396 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2488 2929 3294 3505 4266 5294 Size: 275 186 487 441 365 211 761 1028 Cuts at: 5294 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 487 441 365 275 211 186 BsiHKAI G_wGCw'C Cuts at: 2022 2871 4116 4201 5272 2022 Size: 849 1245 85 1071 2805 Fragments arranged by size: 2805 1245 1071 849 85 BsmI GAATG_Cn' Cuts at: 3566 3659 3566 Size: 93 5962 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2022 2438 2450 2517 2550 2781 2854 Size: 399 232 416 12 67 33 231 73 Cuts at: 2854 2865 2871 3350 3374 4116 4201 5272 1391 Size: 11 6 479 24 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 479 416 399 232 231 85 73 67 33 24 12 11 6 BspEI T'CCGG_A Cuts at: 5718 5718 Size: 6055 BspHI T'CATG_A Cuts at: 2493 3944 5674 2493 Size: 1451 1730 2874 Fragments arranged by size: 2874 1730 1451 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2760 3410 844 Size: 1916 650 3489 Fragments arranged by size: 3489 1916 650 BsrBI CCG'CTC Cuts at: 1394 1823 2140 2235 2706 3942 1394 Size: 429 317 95 471 1236 3507 Fragments arranged by size: 3507 1236 471 429 317 95 BsrDI GCAATG_nn' Cuts at: 65 1248 4550 4724 65 Size: 1183 3302 174 1396 Fragments arranged by size: 3302 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2030 2294 4699 4886 1700 Size: 110 220 264 2405 187 2869 Fragments arranged by size: 2869 2405 264 220 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6055 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6055 BssSI C'ACGA_G Cuts at: 4115 5127 4115 Size: 1012 5043 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6055 BstBI TT'CG_AA Cuts at: 4859 4859 Size: 6055 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6055 BstXI CCAn_nnnn'nTGG Cuts at: 2200 2200 Size: 6055 BstYI r'GATC_y Cuts at: 1972 3036 3385 3752 4137 4154 5606 5692 5704 Size: 1064 349 367 385 17 1452 86 12 Cuts at: 5704 6050 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1064 385 367 349 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2220 2923 3079 3288 513 Size: 552 1155 703 156 209 3280 Fragments arranged by size: 3280 1155 703 552 209 156 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5316 Fragments arranged by size: 5316 683 56 BtsI GCAGTG_nn' Cuts at: 3571 4365 4385 3571 Size: 794 20 5241 Fragments arranged by size: 5241 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6055 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6020 DraI TTT'AAA Cuts at: 3359 3359 Size: 6055 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5062 809 Size: 507 3746 1802 Fragments arranged by size: 3746 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2032 2655 2694 Size: 54 1425 50 275 18 202 623 39 Cuts at: 2694 2763 2904 2926 2943 2997 3057 3120 3162 Size: 69 141 22 17 54 60 63 42 Cuts at: 3162 3291 3502 4391 6020 8 Size: 129 211 889 1629 43 Fragments arranged by size: 1629 1425 889 623 275 211 202 141 129 69 63 60 54 54 50 43 42 39 22 18 17 EagI C'GGCC_G Cuts at: 1537 1812 2926 3291 3502 1537 Size: 275 1114 365 211 4090 Fragments arranged by size: 4090 1114 365 275 211 EarI CTCTTCn'nnn_ Cuts at: 1353 2173 2261 3294 3985 1353 Size: 820 88 1033 691 3423 Fragments arranged by size: 3423 1033 820 691 88 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2348 4642 5016 5162 387 Size: 1064 138 759 2294 374 146 1280 Fragments arranged by size: 2294 1280 1064 759 374 146 138 EcoICRI GAG'CTC Cuts at: 2869 2869 Size: 6055 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2156 2242 2488 4118 4715 5502 5770 Size: 393 524 86 246 1630 597 787 268 Cuts at: 5770 1239 Size: 1524 Fragments arranged by size: 1630 1524 787 597 524 393 268 246 86 EcoO109I rG'GnC_Cy Cuts at: 2241 2674 2851 3346 3347 2241 Size: 433 177 495 1 4949 Fragments arranged by size: 4949 495 433 177 1 EcoRI G'AATT_C Cuts at: 3364 3742 3364 Size: 378 5677 FseI GG_CCGG'CC Cuts at: 2034 2034 Size: 6055 FspI TGC'GCA Cuts at: 2756 4561 2756 Size: 1805 4250 HaeII r_GCGC'y Cuts at: 1678 2624 5202 1678 Size: 946 2578 2531 Fragments arranged by size: 2578 2531 946 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3245 3277 3317 3349 4696 Size: 32 135 32 1673 32 40 32 1347 Cuts at: 4696 4728 4770 4802 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1673 1347 135 42 40 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2770 3399 3645 669 Size: 988 281 832 629 246 3079 Fragments arranged by size: 3079 988 832 629 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6055 HpaI GTT'AAC Cuts at: 3645 3645 Size: 6055 KpnI G_GTAC'C Cuts at: 3380 3380 Size: 6055 MfeI C'AATT_G Cuts at: 3654 3654 Size: 6055 MluI A'CGCG_T Cuts at: 5931 5931 Size: 6055 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3315 5169 5353 1305 Size: 2010 1854 184 2007 Fragments arranged by size: 2010 2007 1854 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2657 2945 2999 10 Size: 54 1425 343 825 288 54 3066 Fragments arranged by size: 3066 1425 825 343 288 54 54 MslI CAynn'nnrTG Cuts at: 518 1166 2198 2540 2969 3164 4013 4372 4531 Size: 648 1032 342 429 195 849 359 159 Cuts at: 4531 518 Size: 2042 Fragments arranged by size: 2042 1032 849 648 429 359 342 195 159 MspA1I CmG'CkG Cuts at: 1606 2103 2134 2407 2578 3081 3471 4148 5296 Size: 497 31 273 171 503 390 677 1148 Cuts at: 5296 5541 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 503 497 390 273 245 171 31 NaeI GCC'GGC Cuts at: 2032 2296 2032 Size: 264 5791 NcoI C'CATG_G Cuts at: 513 1065 2220 513 Size: 552 1155 4348 Fragments arranged by size: 4348 1155 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6055 NgoMIV G'CCGG_C Cuts at: 2030 2294 2030 Size: 264 5791 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6055 NotI GC'GGCC_GC Cuts at: 3502 3502 Size: 6055 NspI r_CATG'y Cuts at: 1132 1229 2968 3413 4958 1132 Size: 97 1739 445 1545 2229 Fragments arranged by size: 2229 1739 1545 445 97 PasI CC'CwG_GG Cuts at: 1348 3343 1348 Size: 1995 4060 PciI A'CATG_T Cuts at: 4954 4954 Size: 6055 PflMI CCAn_nnn'nTGG Cuts at: 1108 2064 2419 2578 2581 1108 Size: 956 355 159 3 4582 Fragments arranged by size: 4582 956 355 159 3 PfoI T'CCnGG_A Cuts at: 2352 3126 2352 Size: 774 5281 PmeI GTTT'AAAC Cuts at: 3359 3359 Size: 6055 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3209 3241 4124 4156 5637 5669 3209 Size: 32 883 32 1481 32 3595 Fragments arranged by size: 3595 1481 883 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2674 2674 Size: 6055 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6055 PsiI TTA'TAA Cuts at: 3625 3625 Size: 6055 PspOMI G'GGCC_C Cuts at: 2850 3346 2850 Size: 496 5559 PspXI vC'TCGA_Gb Cuts at: 2187 2187 Size: 6055 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3005 3037 3005 Size: 32 6023 PstI C_TGCA'G Cuts at: 830 2397 3407 830 Size: 1567 1010 3478 Fragments arranged by size: 3478 1567 1010 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6055 PvuII CAG'CTG Cuts at: 2134 2407 2578 2134 Size: 273 171 5611 Fragments arranged by size: 5611 273 171 SacI G_AGCT'C Cuts at: 2871 2871 Size: 6055 SacII CC_GC'GG Cuts at: 3082 3082 Size: 6055 SalI G'TCGA_C Cuts at: 1936 3397 1936 Size: 1461 4594 SbfI CC_TGCA'GG Cuts at: 2397 3407 2397 Size: 1010 5045 ScaI AGT'ACT Cuts at: 4303 6015 4303 Size: 1712 4343 SexAI A'CCwGG_T Cuts at: 2685 3255 2685 Size: 570 5485 SfcI C'TryA_G Cuts at: 826 1045 2393 3403 4538 5219 5410 826 Size: 219 1348 1010 1135 681 191 1471 Fragments arranged by size: 1471 1348 1135 1010 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6055 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6055 SmaI CCC'GGG Cuts at: 1872 2733 2831 3382 1872 Size: 861 98 551 4545 Fragments arranged by size: 4545 861 551 98 SmlI C'TyrA_G Cuts at: 820 1017 1943 2187 2648 3086 4159 5060 5322 Size: 197 926 244 461 438 1073 901 262 Cuts at: 5322 820 Size: 1553 Fragments arranged by size: 1553 1073 926 901 461 438 262 244 197 SnaBI TAC'GTA Cuts at: 493 3846 493 Size: 3353 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6055 SphI G_CATG'C Cuts at: 1132 3413 1132 Size: 2281 3774 SspI AAT'ATT Cuts at: 5 52 3979 5 Size: 47 3927 2081 Fragments arranged by size: 3927 2081 47 StuI AGG'CCT Cuts at: 1517 2165 2263 3182 1517 Size: 648 98 919 4390 Fragments arranged by size: 4390 919 648 98 StyI C'CwwG_G Cuts at: 513 1065 2220 2310 2523 3351 3494 513 Size: 552 1155 90 213 828 143 3074 Fragments arranged by size: 3074 1155 828 552 213 143 90 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2338 2536 2888 4082 4099 4252 4437 Size: 1255 758 198 352 1194 17 153 185 Cuts at: 4437 4882 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1194 758 445 352 198 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4301 6013 96 Size: 274 80 33 51 1443 2324 1712 138 Fragments arranged by size: 2324 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2359 2391 6015 6047 2359 Size: 32 3624 32 2367 Fragments arranged by size: 3624 2367 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2228 1316 Size: 912 5143 XbaI T'CTAG_A Cuts at: 3391 3391 Size: 6055 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2575 2899 2977 2575 Size: 324 78 5653 Fragments arranged by size: 5653 324 78 XhoI C'TCGA_G Cuts at: 1943 2187 1943 Size: 244 5811 XmaI C'CCGG_G Cuts at: 1870 2731 2829 3380 1870 Size: 861 98 551 4545 Fragments arranged by size: 4545 861 551 98 XmnI GAAnn'nnTTC Cuts at: 2501 4184 2501 Size: 1683 4372 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3863 276 Size: 53 83 186 936 2329 2468 Fragments arranged by size: 2468 2329 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FspAI KasI NarI NruI NsiI PacI PmlI RsrII SanDI SapI SfiI SfoI SrfI SwaI