Restriction map for pFN21AE1913
 (Linear) (Six-base) MAPSORT of: KIEE1913.seq  Check: 9646  from: 1  to: 1350



KIEE1913.orf

 With 182 enzymes: SgfI * 

                        July 16, 2019 14:21  ..

AccI GT'mk_AC

Cuts at:      0     146    1350 
   Size:        146    1204
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     237     483    1350 
   Size:        237     246     867

  Fragments arranged by size: 

                 867     246     237
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1254    1350 
   Size:       1254      96
 
AleI CACnn'nnGTG

Cuts at:      0     535    1350 
   Size:        535     815
 
AlwNI CAG_nnn'CTG

Cuts at:      0     990    1128    1350 
   Size:        990     138     222

  Fragments arranged by size: 

                 990     222     138
 
ApaI G_GGCC'C

Cuts at:      0     849    1345    1350 
   Size:        849     496       5

  Fragments arranged by size: 

                 849     496       5
 
ApaLI G'TGCA_C

Cuts at:      0      13    1350 
   Size:         13    1337
 
AvaI C'yCGr_G

Cuts at:      0     182     726     824     859    1350 
   Size:        182     544      98      35     491

  Fragments arranged by size: 

                 544     491     182      98      35
 
BamHI G'GATC_C

Cuts at:      0    1031    1350 
   Size:       1031     319
 
BanI G'GyrC_C

Cuts at:      0     771    1350 
   Size:        771     579
 
BanII G_rGCy'C

Cuts at:      0     433     545     849     866    1345    1350 
   Size:        433     112     304      17     479       5

  Fragments arranged by size: 

                 479     433     304     112      17       5
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1319    1350 
   Size:       1319      31
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      27      61    1002    1036    1350 
   Size:         27      34     941      34     314

  Fragments arranged by size: 

                 941     314      34      34      27
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     485    1350 
   Size:        485     865
 
BglI GCCn_nnn'nGGC

Cuts at:      0      35     392    1350 
   Size:         35     357     958

  Fragments arranged by size: 

                 958     357      35
 
Bme1580I G_kGCm'C

Cuts at:      0      17     445     512     776     849     860    1345    1350 
   Size:         17     428      67     264      73      11     485       5

  Fragments arranged by size: 

                 485     428     264      73      67      17      11       5
            
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     309    1350 
   Size:        309    1041
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     151    1350 
   Size:        151    1199
 
Bpu10I CC'TnA_GC

Cuts at:      0     231    1350 
   Size:        231    1119
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     628    1066    1350 
   Size:        628     438     284

  Fragments arranged by size: 

                 628     438     284
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     586    1350 
   Size:        586     764
 
BsaAI yAC'GTr

Cuts at:      0     108     177    1350 
   Size:        108      69    1173

  Fragments arranged by size: 

                1173     108      69
 
BsaHI Gr'CG_yC

Cuts at:      0    1280    1350 
   Size:       1280      70
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     484    1336    1339    1350 
   Size:        484     852       3      11

  Fragments arranged by size: 

                 852     484      11       3
 
BseYI C'CCAG_C

Cuts at:      0      94     414     869    1350 
   Size:         94     320     455     481

  Fragments arranged by size: 

                 481     455     320      94
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     772    1168    1350 
   Size:        772     396     182

  Fragments arranged by size: 

                 772     396     182
 
BsiEI CG_ry'CG

Cuts at:      0     483     924    1289    1350 
   Size:        483     441     365      61

  Fragments arranged by size: 

                 483     441     365      61
 
BsiHKAI G_wGCw'C

Cuts at:      0      17     866    1350 
   Size:         17     849     484

  Fragments arranged by size: 

                 849     484      17
 
Bsp1286I G_dGCh'C

Cuts at:      0      17     433     445     512     545     776     849     860 
   Size:         17     416      12      67      33     231      73      11

Cuts at:    860     866    1345    1350 
   Size:          6     479       5

  Fragments arranged by size: 

                 479     416     231      73      67      33      17      12
                  11       6       5
 
BspHI T'CATG_A

Cuts at:      0     488    1350 
   Size:        488     862
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     755    1350 
   Size:        755     595
 
BsrBI CCG'CTC

Cuts at:      0     135     230     701    1350 
   Size:        135      95     471     649

  Fragments arranged by size: 

                 649     471     135      95
 
BsrFI r'CCGG_y

Cuts at:      0      25     289    1350 
   Size:         25     264    1061

  Fragments arranged by size: 

                1061     264      25
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     195    1350 
   Size:        195    1155
 
BstYI r'GATC_y

Cuts at:      0    1031    1350 
   Size:       1031     319
 
BtgI C'CryG_G

Cuts at:      0     215     918    1074    1283    1350 
   Size:        215     703     156     209      67

  Fragments arranged by size: 

                 703     215     209     156      67
 
EaeI y'GGCC_r

Cuts at:      0      27     650     689     758     899     921     938     992 
   Size:         27     623      39      69     141      22      17      54

Cuts at:    992    1052    1115    1157    1286    1350 
   Size:         60      63      42     129      64

  Fragments arranged by size: 

                 623     141     129      69      64      63      60      54
                  42      39      27      22      17
 
EagI C'GGCC_G

Cuts at:      0     921    1286    1350 
   Size:        921     365      64

  Fragments arranged by size: 

                 921     365      64
 
EarI CTCTTCn'nnn_

Cuts at:      0     168     256    1289    1350 
   Size:        168      88    1033      61

  Fragments arranged by size: 

                1033     168      88      61
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     343    1350 
   Size:        343    1007
 
EcoICRI GAG'CTC

Cuts at:      0     864    1350 
   Size:        864     486
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     151     237     483    1350 
   Size:        151      86     246     867

  Fragments arranged by size: 

                 867     246     151      86
 
EcoO109I rG'GnC_Cy

Cuts at:      0     236     669     846    1341    1342    1350 
   Size:        236     433     177     495       1       8

  Fragments arranged by size: 

                 495     433     236     177       8       1
 
FseI GG_CCGG'CC

Cuts at:      0      29    1350 
   Size:         29    1321
 
FspI TGC'GCA

Cuts at:      0     751    1350 
   Size:        751     599
 
HaeII r_GCGC'y

Cuts at:      0     619    1350 
   Size:        619     731
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1240    1272    1312    1344    1350 
   Size:       1240      32      40      32       6

  Fragments arranged by size: 

                1240      40      32      32       6
 
HincII GTy'rAC

Cuts at:      0     765    1350 
   Size:        765     585
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1310    1350 
   Size:       1310      40
 
MscI TGG'CCA

Cuts at:      0     652     940     994    1350 
   Size:        652     288      54     356

  Fragments arranged by size: 

                 652     356     288      54
 
MslI CAynn'nnrTG

Cuts at:      0     193     535     964    1159    1350 
   Size:        193     342     429     195     191

  Fragments arranged by size: 

                 429     342     195     193     191
 
MspA1I CmG'CkG

Cuts at:      0      98     129     402     573    1076    1350 
   Size:         98      31     273     171     503     274

  Fragments arranged by size: 

                 503     274     273     171      98      31
 
NaeI GCC'GGC

Cuts at:      0      27     291    1350 
   Size:         27     264    1059

  Fragments arranged by size: 

                1059     264      27
 
NcoI C'CATG_G

Cuts at:      0     215    1350 
   Size:        215    1135
 
NgoMIV G'CCGG_C

Cuts at:      0      25     289    1350 
   Size:         25     264    1061

  Fragments arranged by size: 

                1061     264      25
 
NspI r_CATG'y

Cuts at:      0     963    1350 
   Size:        963     387
 
PasI CC'CwG_GG

Cuts at:      0    1338    1350 
   Size:       1338      12
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      59     414     573     576    1350 
   Size:         59     355     159       3     774

  Fragments arranged by size: 

                 774     355     159      59       3
 
PfoI T'CCnGG_A

Cuts at:      0     347    1121    1350 
   Size:        347     774     229

  Fragments arranged by size: 

                 774     347     229
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1204    1236    1350 
   Size:       1204      32     114

  Fragments arranged by size: 

                1204     114      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     669    1350 
   Size:        669     681
 
PspOMI G'GGCC_C

Cuts at:      0     845    1341    1350 
   Size:        845     496       9

  Fragments arranged by size: 

                 845     496       9
 
PspXI vC'TCGA_Gb

Cuts at:      0     182    1350 
   Size:        182    1168
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1000    1032    1350 
   Size:       1000      32     318

  Fragments arranged by size: 

                1000     318      32
 
PstI C_TGCA'G

Cuts at:      0     392    1350 
   Size:        392     958
 
PvuII CAG'CTG

Cuts at:      0     129     402     573    1350 
   Size:        129     273     171     777

  Fragments arranged by size: 

                 777     273     171     129
 
SacI G_AGCT'C

Cuts at:      0     866    1350 
   Size:        866     484
 
SacII CC_GC'GG

Cuts at:      0    1077    1350 
   Size:       1077     273
 
SbfI CC_TGCA'GG

Cuts at:      0     392    1350 
   Size:        392     958
 
SexAI A'CCwGG_T

Cuts at:      0     680    1250    1350 
   Size:        680     570     100

  Fragments arranged by size: 

                 680     570     100
 
SfcI C'TryA_G

Cuts at:      0     388    1350 
   Size:        388     962
 
SmaI CCC'GGG

Cuts at:      0     728     826    1350 
   Size:        728      98     524

  Fragments arranged by size: 

                 728     524      98
 
SmlI C'TyrA_G

Cuts at:      0     182     643    1081    1350 
   Size:        182     461     438     269

  Fragments arranged by size: 

                 461     438     269     182
 
StuI AGG'CCT

Cuts at:      0     160     258    1177    1350 
   Size:        160      98     919     173

  Fragments arranged by size: 

                 919     173     160      98
 
StyI C'CwwG_G

Cuts at:      0     215     305     518    1350 
   Size:        215      90     213     832

  Fragments arranged by size: 

                 832     215     213      90
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     333     531     883    1350 
   Size:        333     198     352     467

  Fragments arranged by size: 

                 467     352     333     198
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     354     386    1350 
   Size:        354      32     964

  Fragments arranged by size: 

                 964     354      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     223    1350 
   Size:        223    1127
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     570     894     972    1350 
   Size:        570     324      78     378

  Fragments arranged by size: 

                 570     378     324      78
 
XhoI C'TCGA_G

Cuts at:      0     182    1350 
   Size:        182    1168
 
XmaI C'CCGG_G

Cuts at:      0     726     824    1350 
   Size:        726      98     526

  Fragments arranged by size: 

                 726     526      98
 
XmnI GAAnn'nnTTC

Cuts at:      0     496    1350 
   Size:        496     854
 

 Enzymes that do cut:

     AccI     AcuI     AhdI     AleI    AlwNI     ApaI    ApaLI     AvaI
    BamHI     BanI    BanII     BbsI     BcgI    BciVI     BglI Bme1580I
     BmrI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspHI    BspMI    BsrBI
    BsrFI    BstXI    BstYI     BtgI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI EcoO109I     FseI     FspI    HaeII    Hin4I   HincII
     MmeI     MscI     MslI   MspA1I     NaeI     NcoI   NgoMIV     NspI
     PasI    PflMI     PfoI     PpiI    PpuMI   PspOMI    PspXI     PsrI
     PstI    PvuII     SacI    SacII     SbfI    SexAI     SfcI     SmaI
     SmlI     StuI     StyI    TaqII     TstI  Tth111I     XcmI     XhoI
     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AlfI     AloI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI
     BbeI    BbvCI     BclI    BfrBI    BglII     BlpI    BmgBI     BmtI
     BplI    BsaBI    BsaWI    BsaXI    BsiWI     BsmI    BsmBI    BspEI
    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I
   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
    EcoNI    EcoRI    EcoRV     FalI    FspAI  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     NarI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PciI     PmeI     PmlI    PshAI     PsiI     PvuI
    RsrII     SalI    SanDI     SapI     ScaI     SfiI     SfoI     SgfI
    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI     TatI
     XbaI     ZraI
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