################################## # NO CUT ENZYMES ( 193 enzymes) # ################################## AatII AccI AccII AccIII AciI AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspHI AspI AviII AvrII BalI BamHI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmAI BsmBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DpnII DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaI KasI Ksp632I KspI MaeI MamI MboI MfeI MflI MluI MluNI MroI MscI MslI MspA1I MunI MvnI NaeI NarI NcoI NdeI NdeII NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau3AI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TfiI Tsp509I Tth111I Van91I XbaI XcmI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### Acc65I 149 GGTACC G^GTACC Asp718I 149 GGTACC G^GTACC AvaI 71 CTCGAG C^[CT]CG[GA]G BanI 149 GGTACC G^G[CT][GA]CC BbsI 141 GAAGAC GAAGAC(2/6) BcnI 90 CCGGG CC^[GC]GG BpuAI 141 GAAGAC GAAGAC(2/6) BseRI 34 CTCCTC GAGGAG(10/8) BsgI 199 GTGCAG GTGCAG(16/14) BsmFI 76 GGGAC GGGAC(10/14) BsmI 246 GAATGC GAATGC(1/-1) BsoBI 71 CTCGAG C^[CT]CG[GA]G BspMI 260 ACCTGC ACCTGC(4/8) BstEII 27 GGTCACC G^GT[ACGT]ACC BstPI 27 GGTCACC G^GT[ACGT]ACC Cac8I 18 GCTGGC GC[ACGT]^[ACGT]GC EcoO65I 27 GGTCACC G^GT[ACGT]ACC HapII 90 CCGG C^CGG HpaII 90 CCGG C^CGG HphI 29 TCACC GGTGA(8/7) KpnI 149 GGTACC GGTAC^C MaeII 145 ACGT A^CGT MseI 254 TTAA T^TAA MspI 90 CCGG C^CGG MwoI 89 GCCGGGAGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NciI 90 CCGGG CC^[GC]GG PaeR7I 71 CTCGAG C^TCGAG PstI 52 CTGCAG CTGCA^G ScaI 107 AGTACT AGT^ACT SfcI 52 CTGCAG C^T[GA][CT]AG TaqI 72 TCGA T^CGA Tru9I 254 TTAA T^TAA Tsp45I 28 GTCAC ^GT[GC]AC TspRI 8 CACTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 72 TCGA T^CGA XhoI 71 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### AfaI 108 GTAC GT^AC 150 GTAC AluI 69 AGCT AG^CT 137 AGCT AvaII 77 GGACC G^G[AT]CC 222 GGACC BpmI 171 CTCCAG CTGGAG(16/14) 103 CTGGAG BsiYI 33 CCTCCTCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 36 CCTCCTGGGGG BslI 33 CCTCCTCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 36 CCTCCTGGGGG BstF5I 60 CATCC GGATG(2/0) 177 GGATG DdeI 130 CTTAG C^T[ACGT]AG 161 CTGAG FokI 60 CATCC GGATG(9/13) 177 GGATG MaeIII 28 GTCAC ^GT[ACGT]AC 193 GTAAC RsaI 108 GTAC GT^AC 150 GTAC SfaNI 15 GATGC GCATC(5/9) 178 GATGC SmlI 71 CTCGAG C^T[CT][GA]AG 271 CTTGAG ######################################### # 3 OR MORE CUT ENZYMES ( 16 enzymes) # ######################################### BbvI BsaJI BssKI BstNI Cfr13I EcoRII Fnu4HI HaeIII ItaI MboII MnlI MvaI NlaIV Sau96I ScrFI TseI