################################## # NO CUT ENZYMES ( 146 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AcyI AfaI AflII AflIII AgeI AlwNI AocI Aor51HI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AspI AviII AvrII BalI BamHI BanI BbeI BbrPI BbvCI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaHI BsiWI BsmBI BsmI Bsp1407I BspHI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstNI BstPI BstZ17I Bsu36I CelII Cfr10I CpoI DraI DraIII DrdI EagI EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRII EcoRV EcoT22I FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MfeI MluI MluNI MscI MspA1I MunI MvaI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AccII 411 CGCG CG^CG AccIII 692 TCCGGA T^CCGGA AhdI 392 GACACAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AluI 297 AGCT AG^CT Alw44I 589 GTGCAC G^TGCAC AlwI 728 GATCC GGATC(4/5) ApaLI 589 GTGCAC G^TGCAC AspEI 392 GACACAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 589 GTGCAC G[AT]GC[AT]^C BbsI 316 GTCTTC GAAGAC(2/6) BciVI 20 GTATCC GTATCC(6/5) BclI 32 TGATCA T^GATCA BfaI 205 CTAG C^TAG BpmI 309 CTCCAG CTGGAG(16/14) BpuAI 316 GTCTTC GAAGAC(2/6) BsaBI 27 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 692 TCCGGA [AT]^CCGG[AT] BseAI 692 TCCGGA T^CCGGA BseRI 835 CTCCTC GAGGAG(10/8) BsgI 117 GTGCAG GTGCAG(16/14) BsiEI 846 CGACCG CG[GA][CT]^CG BsiHKAI 589 GTGCAC G[AT]GC[AT]^C BspEI 692 TCCGGA T^CCGGA BspMI 196 ACCTGC ACCTGC(4/8) BsrDI 75 CATTGC GCAATG(2/0) BstUI 411 CGCG CG^CG BstXI 74 CCATTGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 727 AGATCC [GA]^GATC[CT] DsaI 824 CCATGG C^C[GA][CT]GG EaeI 920 CGGCCA [CT]^GGCC[GA] Eam1105I 392 GACACAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoT14I 824 CCATGG C^C[AT][AT]GG FbaI 32 TGATCA T^GATCA MaeI 205 CTAG C^TAG MaeII 398 ACGT A^CGT MamI 27 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 727 AGATCC [GA]^GATC[CT] MroI 692 TCCGGA T^CCGGA MvnI 411 CGCG CG^CG NcoI 824 CCATGG C^CATGG NlaIII 825 CATG CATG^ PstI 431 CTGCAG CTGCA^G StyI 824 CCATGG C^C[AT][AT]GG TfiI 689 GATTC G^A[AT]TC XhoII 727 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### BanII 355 GGGCTC G[GA]GC[CT]^C 721 GAGCCC BcgI 220 CGAGGCGTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 230 GCAAGAAAATCG BsaI 485 GAGACC GGTCTC(1/5) 734 GAGACC BspDI 238 ATCGAT AT^CGAT 686 ATCGAT CfoI 410 GCGC GCG^C 930 GCGC ClaI 238 ATCGAT AT^CGAT 686 ATCGAT DdeI 328 CTCAG C^T[ACGT]AG 406 CTCAG HaeIII 306 GGCC GG^CC 921 GGCC HhaI 410 GCGC GCG^C 930 GCGC HinP1I 410 GCGC G^CGC 930 GCGC MseI 67 TTAA T^TAA 439 TTAA MslI 75 CATTGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 783 CATCTCGATG PleI 502 GACTC GAGTC(4/5) 901 GAGTC SmaI 490 CCCGGG CCC^GGG 803 CCCGGG Tru9I 67 TTAA T^TAA 439 TTAA Tsp509I 570 AATT ^AATT 877 AATT XmaCI 490 CCCGGG C^CCGGG 803 CCCGGG XmaI 490 CCCGGG C^CCGGG 803 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 49 enzymes) # ######################################### AciI AvaI AvaII BbvI BcnI BmyI BsaJI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I Cac8I Cfr13I DpnII DraII EcoO109I FauI Fnu4HI FokI HapII HinfI HpaII HphI ItaI MaeIII MboI MboII MnlI MspI MwoI NciI NdeII NlaIV PpuMI Sau3AI Sau96I ScrFI SfaNI SfcI TaqI TseI Tsp45I TspRI TthHB8I