Restriction map for pFN21AE5420
 (Linear) (Six-base) MAPSORT of: KIEE5420.seq  Check: 6694  from: 1  to: 945



KIEE5420.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:59  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     272     667     945 
   Size:        272     395     278

  Fragments arranged by size: 

                 395     278     272
 
AhdI GACnn_n'nnGTC

Cuts at:      0     397     945 
   Size:        397     548
 
ApaLI G'TGCA_C

Cuts at:      0     589     945 
   Size:        589     356
 
AvaI C'yCGr_G

Cuts at:      0     490     731     803     945 
   Size:        490     241      72     142

  Fragments arranged by size: 

                 490     241     142      72
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0       3      36     945 
   Size:          3      33     909

  Fragments arranged by size: 

                 909      33       3
 
BanII G_rGCy'C

Cuts at:      0     359     725     945 
   Size:        359     366     220

  Fragments arranged by size: 

                 366     359     220
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     309     945 
   Size:        309     636
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     209     219     243     253     945 
   Size:        209      10      24      10     692

  Fragments arranged by size: 

                 692     209      24      10      10
 
BciVI GTATCCnnnnn_n'

Cuts at:      0      31     945 
   Size:         31     914
 
BclI T'GATC_A

Cuts at:      0      32     945 
   Size:         32     913
 
Bme1580I G_kGCm'C

Cuts at:      0      88     593     945 
   Size:         88     505     352

  Fragments arranged by size: 

                 505     352      88
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     294     945 
   Size:        294     651
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     479     728     945 
   Size:        479     249     217

  Fragments arranged by size: 

                 479     249     217
 
BsaBI GATnn'nnATC

Cuts at:      0      31     945 
   Size:         31     914
 
BsaWI w'CCGG_w

Cuts at:      0     692     945 
   Size:        692     253
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     826     945 
   Size:        826     119
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     138     945 
   Size:        138     807
 
BsiEI CG_ry'CG

Cuts at:      0     849     945 
   Size:        849      96
 
BsiHKAI G_wGCw'C

Cuts at:      0     593     945 
   Size:        593     352
 
Bsp1286I G_dGCh'C

Cuts at:      0      88     359     593     725     945 
   Size:         88     271     234     132     220

  Fragments arranged by size: 

                 271     234     220     132      88
 
BspEI T'CCGG_A

Cuts at:      0     692     945 
   Size:        692     253
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     205     945 
   Size:        205     740
 
BsrDI GCAATG_nn'

Cuts at:      0      74     945 
   Size:         74     871
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      81     945 
   Size:         81     864
 
BstYI r'GATC_y

Cuts at:      0     727     945 
   Size:        727     218
 
BtgI C'CryG_G

Cuts at:      0     824     945 
   Size:        824     121
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     546     945 
   Size:        546     399
 
BtsI GCAGTG_nn'

Cuts at:      0      86     904     945 
   Size:         86     818      41

  Fragments arranged by size: 

                 818      86      41
 
ClaI AT'CG_AT

Cuts at:      0     239     687     945 
   Size:        239     448     258

  Fragments arranged by size: 

                 448     258     239
 
EaeI y'GGCC_r

Cuts at:      0     920     945 
   Size:        920      25
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     272     294     667     945 
   Size:        272      22     373     278

  Fragments arranged by size: 

                 373     278     272      22
 
EcoO109I rG'GnC_Cy

Cuts at:      0     194     305     807     830     945 
   Size:        194     111     502      23     115

  Fragments arranged by size: 

                 502     194     115     111      23
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     383     415     712     744     945 
   Size:        383      32     297      32     201

  Fragments arranged by size: 

                 383     297     201      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     869     945 
   Size:        869      76
 
MslI CAynn'nnrTG

Cuts at:      0      79     787     945 
   Size:         79     708     158

  Fragments arranged by size: 

                 708     158      79
 
NcoI C'CATG_G

Cuts at:      0     824     945 
   Size:        824     121
 
PpuMI rG'GwC_Cy

Cuts at:      0     194     807     830     945 
   Size:        194     613      23     115

  Fragments arranged by size: 

                 613     194     115      23
 
PstI C_TGCA'G

Cuts at:      0     435     945 
   Size:        435     510
 
SanDI GG'GwC_CC

Cuts at:      0     807     830     945 
   Size:        807      23     115

  Fragments arranged by size: 

                 807     115      23
 
SfcI C'TryA_G

Cuts at:      0      41     431     678     865     945 
   Size:         41     390     247     187      80

  Fragments arranged by size: 

                 390     247     187      80      41
 
SmaI CCC'GGG

Cuts at:      0     492     805     945 
   Size:        492     313     140

  Fragments arranged by size: 

                 492     313     140
 
StyI C'CwwG_G

Cuts at:      0     824     945 
   Size:        824     121
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     900     945 
   Size:        900      45
 
XmaI C'CCGG_G

Cuts at:      0     490     803     945 
   Size:        490     313     142

  Fragments arranged by size: 

                 490     313     142
 

 Enzymes that do cut:

     AcuI     AhdI    ApaLI     AvaI     BaeI    BanII     BbsI     BcgI
    BciVI     BclI Bme1580I     BpmI     BsaI    BsaBI    BsaWI    BseRI
     BsgI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspMI    BsrDI    BstXI
    BstYI     BtgI    BtgZI     BtsI     ClaI     EaeI  Eco57MI EcoO109I
    Hin4I     MmeI     MslI     NcoI    PpuMI     PstI    SanDI     SfcI
     SmaI     StyI    TaqII     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AleI     AlfI     AloI    AlwNI     ApaI     ApoI     AscI
     AseI    AsiSI    AvrII    BamHI     BanI     BbeI    BbvCI    BfrBI
     BglI    BglII     BlpI    BmgBI     BmrI     BmtI     BplI   Bpu10I
    BpuEI    BsaAI    BsaHI    BsaXI    BseYI    BsiWI     BsmI    BsmBI
    BspHI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII  BstZ17I   Bsu36I    CspCI     DraI   DraIII     DrdI     EagI
     EarI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MscI   MspA1I     NaeI     NarI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmnI     ZraI
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