(Linear) (Six-base) MAPSORT of: KIEE5420.seq Check: 6694 from: 1 to: 945 KIEE5420.orf With 182 enzymes: SgfI * January 29, 2014 11:59 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 272 667 945 Size: 272 395 278 Fragments arranged by size: 395 278 272 AhdI GACnn_n'nnGTC Cuts at: 0 397 945 Size: 397 548 ApaLI G'TGCA_C Cuts at: 0 589 945 Size: 589 356 AvaI C'yCGr_G Cuts at: 0 490 731 803 945 Size: 490 241 72 142 Fragments arranged by size: 490 241 142 72 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 3 36 945 Size: 3 33 909 Fragments arranged by size: 909 33 3 BanII G_rGCy'C Cuts at: 0 359 725 945 Size: 359 366 220 Fragments arranged by size: 366 359 220 BbsI GAAGACnn'nnnn_ Cuts at: 0 309 945 Size: 309 636 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 209 219 243 253 945 Size: 209 10 24 10 692 Fragments arranged by size: 692 209 24 10 10 BciVI GTATCCnnnnn_n' Cuts at: 0 31 945 Size: 31 914 BclI T'GATC_A Cuts at: 0 32 945 Size: 32 913 Bme1580I G_kGCm'C Cuts at: 0 88 593 945 Size: 88 505 352 Fragments arranged by size: 505 352 88 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 294 945 Size: 294 651 BsaI GGTCTCn'nnnn_ Cuts at: 0 479 728 945 Size: 479 249 217 Fragments arranged by size: 479 249 217 BsaBI GATnn'nnATC Cuts at: 0 31 945 Size: 31 914 BsaWI w'CCGG_w Cuts at: 0 692 945 Size: 692 253 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 826 945 Size: 826 119 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 138 945 Size: 138 807 BsiEI CG_ry'CG Cuts at: 0 849 945 Size: 849 96 BsiHKAI G_wGCw'C Cuts at: 0 593 945 Size: 593 352 Bsp1286I G_dGCh'C Cuts at: 0 88 359 593 725 945 Size: 88 271 234 132 220 Fragments arranged by size: 271 234 220 132 88 BspEI T'CCGG_A Cuts at: 0 692 945 Size: 692 253 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 205 945 Size: 205 740 BsrDI GCAATG_nn' Cuts at: 0 74 945 Size: 74 871 BstXI CCAn_nnnn'nTGG Cuts at: 0 81 945 Size: 81 864 BstYI r'GATC_y Cuts at: 0 727 945 Size: 727 218 BtgI C'CryG_G Cuts at: 0 824 945 Size: 824 121 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 546 945 Size: 546 399 BtsI GCAGTG_nn' Cuts at: 0 86 904 945 Size: 86 818 41 Fragments arranged by size: 818 86 41 ClaI AT'CG_AT Cuts at: 0 239 687 945 Size: 239 448 258 Fragments arranged by size: 448 258 239 EaeI y'GGCC_r Cuts at: 0 920 945 Size: 920 25 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 272 294 667 945 Size: 272 22 373 278 Fragments arranged by size: 373 278 272 22 EcoO109I rG'GnC_Cy Cuts at: 0 194 305 807 830 945 Size: 194 111 502 23 115 Fragments arranged by size: 502 194 115 111 23 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 383 415 712 744 945 Size: 383 32 297 32 201 Fragments arranged by size: 383 297 201 32 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 869 945 Size: 869 76 MslI CAynn'nnrTG Cuts at: 0 79 787 945 Size: 79 708 158 Fragments arranged by size: 708 158 79 NcoI C'CATG_G Cuts at: 0 824 945 Size: 824 121 PpuMI rG'GwC_Cy Cuts at: 0 194 807 830 945 Size: 194 613 23 115 Fragments arranged by size: 613 194 115 23 PstI C_TGCA'G Cuts at: 0 435 945 Size: 435 510 SanDI GG'GwC_CC Cuts at: 0 807 830 945 Size: 807 23 115 Fragments arranged by size: 807 115 23 SfcI C'TryA_G Cuts at: 0 41 431 678 865 945 Size: 41 390 247 187 80 Fragments arranged by size: 390 247 187 80 41 SmaI CCC'GGG Cuts at: 0 492 805 945 Size: 492 313 140 Fragments arranged by size: 492 313 140 StyI C'CwwG_G Cuts at: 0 824 945 Size: 824 121 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 900 945 Size: 900 45 XmaI C'CCGG_G Cuts at: 0 490 803 945 Size: 490 313 142 Fragments arranged by size: 490 313 142 Enzymes that do cut: AcuI AhdI ApaLI AvaI BaeI BanII BbsI BcgI BciVI BclI Bme1580I BpmI BsaI BsaBI BsaWI BseRI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspMI BsrDI BstXI BstYI BtgI BtgZI BtsI ClaI EaeI Eco57MI EcoO109I Hin4I MmeI MslI NcoI PpuMI PstI SanDI SfcI SmaI StyI TaqII XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AleI AlfI AloI AlwNI ApaI ApoI AscI AseI AsiSI AvrII BamHI BanI BbeI BbvCI BfrBI BglI BglII BlpI BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaAI BsaHI BsaXI BseYI BsiWI BsmI BsmBI BspHI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I CspCI DraI DraIII DrdI EagI EarI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI MscI MspA1I NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI TstI Tth111I XbaI XcmI XhoI XmnI ZraI