################################## # NO CUT ENZYMES ( 160 enzymes) # ################################## AatII AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI AscI AseI AsnI AspEI AspI AvaI AvaII AviII AvrII BalI BamHI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglI BlnI BlpI Bpu1102I BsaBI BsaHI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BsmFI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HapII HhaI Hin1I HinP1I HpaI HpaII KasI KspI MamI MluI MluNI MroI MscI MslI MspI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tth111I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 30 enzymes) # ################################### Acc65I 1104 GGTACC G^GTACC AccI 1464 GTCGAC GT^[AC][GT]AC Asp700I 1412 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 1104 GGTACC G^GTACC AspHI 670 GAGCAC G[AT]GC[AT]^C BglII 829 AGATCT A^GATCT BmyI 670 GAGCAC G[AGT]GC[ACT]^C BsaAI 641 TACGTA [CT]AC^GT[GA] BsgI 776 CTGCAC GTGCAG(16/14) BsiHKAI 670 GAGCAC G[AT]GC[AT]^C BsmI 659 GAATGC GAATGC(1/-1) Bsp1286I 670 GAGCAC G[AGT]GC[ACT]^C BsrDI 136 CATTGC GCAATG(2/0) BssSI 1205 CTCGTG CACGAG(-5/-1) BstXI 724 CCAGAGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 829 AGATCT [GA]^GATC[CT] DraI 351 TTTAAA TTT^AAA DraIII 1202 CACCTCGTG CAC[ACGT][ACGT][ACGT]^GTG EcoRV 280 GATATC GAT^ATC HincII 1464 GTCGAC GT[CT]^[GA]AC HindII 1464 GTCGAC GT[CT]^[GA]AC KpnI 1104 GGTACC GGTAC^C MfeI 807 CAATTG C^AATTG MflI 829 AGATCT [GA]^GATC[CT] MunI 807 CAATTG C^AATTG NspI 1155 ACATGC [GA]CATG^[CT] SalI 1464 GTCGAC G^TCGAC SnaBI 641 TACGTA TAC^GTA XhoII 829 AGATCT [GA]^GATC[CT] XmnI 1412 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AfaI 1105 GTAC GT^AC 1456 GTAC BanI 963 GGCACC G^G[CT][GA]CC 1104 GGTACC BfaI 926 CTAG C^TAG 1397 CTAG BsaI 1240 GAGACC GGTCTC(1/5) 1264 GAGACC BssKI 146 CCAGG ^CC[ACGT]GG 1270 CCAGG BstF5I 300 GGATG GGATG(2/0) 813 GGATG BstNI 146 CCAGG CC^[AT]GG 1270 CCAGG Cac8I 921 GCAGGC GC[ACGT]^[ACGT]GC 1230 GCCAGC Cfr13I 69 GGCCC G^G[ACGT]CC 1439 GGCCC EcoRII 146 CCAGG ^CC[AT]GG 1270 CCAGG FokI 300 GGATG GGATG(9/13) 813 GGATG HgaI 121 GACGC GACGC(5/10) 132 GACGC HindIII 346 AAGCTT A^AGCTT 1415 AAGCTT MaeI 926 CTAG C^TAG 1397 CTAG MaeIII 650 GTCAC ^GT[ACGT]AC 697 GTCAC MvaI 146 CCAGG CC^[AT]GG 1270 CCAGG PflMI 1021 CCAGAGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1430 CCAAAAAATGG PstI 32 CTGCAG CTGCA^G 1253 CTGCAG PvuII 88 CAGCTG CAG^CTG 1099 CAGCTG RsaI 1105 GTAC GT^AC 1456 GTAC Sau96I 69 GGCCC G^G[ACGT]CC 1439 GGCCC ScrFI 146 CCAGG CC^[ACGT]GG 1270 CCAGG Tsp45I 650 GTCAC ^GT[GC]AC 697 GTCAC Van91I 1021 CCAGAGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1430 CCAAAAAATGG ######################################### # 3 OR MORE CUT ENZYMES ( 44 enzymes) # ######################################### AciI AcsI AluI AlwNI ApoI BbsI BbvI BpmI BpuAI BsaJI BsiYI BslI BsmAI BsrI DdeI DpnII EarI Fnu4HI HaeIII HinfI HphI ItaI Ksp632I MaeII MboI MboII MnlI MseI MspA1I MwoI NdeII NlaIII NlaIV PleI Sau3AI SfaNI SfcI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I