Restriction map for
pFN21AE2393
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# NO CUT ENZYMES ( 126 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AhdI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AviII AvrII BbeI BbrPI
BbvCI BcgI BciVI BclI BfaI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaBI BsaI BseAI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspLU11I BsrDI BsrGI BssSI
Bst1107I BstBI BstZ17I CelII ClaI
CpoI DraI DraIII EagI Eam1105I
EclXI Eco52I EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HinfI HpaI KasI KpnI
KspI MaeI MamI MfeI MluI
MroI MseI MspA1I MunI NarI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PinAI
PleI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RsrII
SacI SacII SalI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TfiI Tru9I
Tsp509I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AccI 385 GTCTAC GT^[AC][GT]AC
AcyI 558 GACGCC G[GA]^CG[CT]C
Alw44I 955 GTGCAC G^TGCAC
AocI 306 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1001 AGCGCT AGC^GCT
ApaI 1471 GGGCCC GGGCC^C
ApaLI 955 GTGCAC G^TGCAC
AspI 544 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1108 CCCGAG C^[CT]CG[GA]G
BamHI 311 GGATCC G^GATCC
BanI 987 GGCACC G^G[CT][GA]CC
BpmI 1196 CTCCAG CTGGAG(16/14)
BsaHI 558 GACGCC G[GA]^CG[CT]C
BsaWI 404 TCCGGT [AT]^CCGG[AT]
BseRI 147 CTCCTC GAGGAG(10/8)
BsmAI 1194 GTCTC GTCTC(1/5)
BsoBI 1108 CCCGAG C^[CT]CG[GA]G
BspMI 764 ACCTGC ACCTGC(4/8)
BstEII 915 GGTCACC G^GT[ACGT]ACC
BstPI 915 GGTCACC G^GT[ACGT]ACC
BstYI 311 GGATCC [GA]^GATC[CT]
Bsu36I 306 CCTGAGG CC^T[ACGT]AGG
DrdI 1330 GACCAGAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1001 AGCGCT AGC^GCT
Eco81I 306 CCTGAGG CC^T[ACGT]AGG
EcoNI 1247 CCTCTGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 915 GGTCACC G^GT[ACGT]ACC
Hin1I 558 GACGCC G[GA]^CG[CT]C
MflI 311 GGATCC [GA]^GATC[CT]
NaeI 1201 GCCGGC GCC^GGC
NgoMIV 1201 GCCGGC G^CCGGC
PflFI 544 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 367 GGGACCT [GA]G^G[AT]CC[CT]
SapI 1428 GCTCTTC GCTCTTC(1/4)
ScaI 473 AGTACT AGT^ACT
SmlI 505 CTCAAG C^T[CT][GA]AG
Tth111I 544 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 563 CCACAGCCAGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 311 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AlwNI 936 CAGCGTCTG CAG[ACGT][ACGT][ACGT]^CTG
1460 CAGCTTCTG
BalI 68 TGGCCA TGG^CCA
746 TGGCCA
BanII 1426 GGGCTC G[GA]GC[CT]^C
1471 GGGCCC
BbsI 1327 GAAGAC GAAGAC(2/6)
757 GTCTTC
BcnI 1065 CCCGG CC^[GC]GG
1212 CCCGG
BpuAI 1327 GAAGAC GAAGAC(2/6)
757 GTCTTC
BsaAI 655 TACGTG [CT]AC^GT[GA]
1357 TACGTG
BsmFI 367 GGGAC GGGAC(10/14)
602 GGGAC
BsrBI 513 CCGCTC CCGCTC(-3/-3)
963 CCGCTC
BsrFI 1201 GCCGGC [GA]^CCGG[CT]
1376 ACCGGC
BsrI 1349 ACTGG ACTGG(1/-1)
590 CCAGT
BssHII 6 GCGCGC G^CGCGC
8 GCGCGC
BstAPI 1014 GCACAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1088 GCAAGTCATGC
Cfr10I 1201 GCCGGC [GA]^CCGG[CT]
1376 ACCGGC
DsaI 905 CCATGG C^C[GA][CT]GG
983 CCATGG
EcoT14I 905 CCATGG C^C[AT][AT]GG
983 CCATGG
MluNI 68 TGGCCA TGG^CCA
746 TGGCCA
MscI 68 TGGCCA TGG^CCA
746 TGGCCA
NciI 1065 CCCGG CC^[GC]GG
1212 CCCGG
NcoI 905 CCATGG C^CATGG
983 CCATGG
PflMI 230 CCAATGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
978 CCACACCATGG
PstI 172 CTGCAG CTGCA^G
1181 CTGCAG
SfaNI 326 GCATC GCATC(5/9)
800 GCATC
SfcI 172 CTGCAG C^T[GA][CT]AG
1181 CTGCAG
StyI 905 CCATGG C^C[AT][AT]GG
983 CCATGG
TaqI 485 TCGA T^CGA
869 TCGA
TthHB8I 485 TCGA T^CGA
869 TCGA
Van91I 230 CCAATGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
978 CCACACCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AspHI AvaII BbvI BmyI BsaJI
BsgI BsiHKAI BsiYI BslI Bsp1286I
BspHI BssKI BstF5I BstNI BstUI
BstXI Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EarI EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MnlI MslI
MspI MvaI MvnI MwoI NdeII
NlaIII NlaIV RcaI RsaI Sau3AI
Sau96I ScrFI TseI Tsp45I TspRI