(Circular) (Six-base) MAPSORT of: pFN21AE2393.seq Check: 2910 from: 1 to: 6199 pFN21AE2393 6199 bp With 182 enzymes: SgfI * August 20, 2012 11:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4009 278 Size: 53 83 186 936 2473 2468 Fragments arranged by size: 2473 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2391 3542 1937 Size: 454 1151 4594 Fragments arranged by size: 4594 1151 454 Acc65I G'GTAC_C Cuts at: 3520 3520 Size: 6199 AclI AA'CG_TT Cuts at: 1563 4326 4699 1563 Size: 2763 373 3063 Fragments arranged by size: 3063 2763 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4262 5646 5914 1632 Size: 2630 1384 268 1917 Fragments arranged by size: 2630 1917 1384 268 AfeI AGC'GCT Cuts at: 3008 3008 Size: 6199 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6002 AflIII A'CryG_T Cuts at: 1203 5098 6075 1203 Size: 3895 977 1327 Fragments arranged by size: 3895 1327 977 AgeI A'CCGG_T Cuts at: 5030 5030 Size: 6199 AhdI GACnn_n'nnGTC Cuts at: 4928 4928 Size: 6199 AleI CACnn'nnGTG Cuts at: 1166 2912 1166 Size: 1746 4453 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2354 2828 1184 Size: 1170 474 4555 Fragments arranged by size: 4555 1170 474 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3371 3403 3371 Size: 32 6167 AlwNI CAG_nnn'CTG Cuts at: 2946 3470 3942 5514 2946 Size: 524 472 1572 3631 Fragments arranged by size: 3631 1572 524 472 ApaI G_GGCC'C Cuts at: 3480 3480 Size: 6199 ApaLI G'TGCA_C Cuts at: 2960 4256 5412 2960 Size: 1296 1156 3747 Fragments arranged by size: 3747 1296 1156 ApoI r'AATT_y Cuts at: 1493 3508 3734 3886 5995 1493 Size: 2015 226 152 2109 1697 Fragments arranged by size: 2109 2015 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4753 160 Size: 872 3721 1606 Fragments arranged by size: 3721 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6199 AvaI C'yCGr_G Cuts at: 1870 1943 3113 3524 1870 Size: 73 1170 411 4545 Fragments arranged by size: 4545 1170 411 73 BamHI G'GATC_C Cuts at: 2316 3529 3896 2316 Size: 1213 367 4619 Fragments arranged by size: 4619 1213 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2992 3520 4975 618 Size: 325 217 627 1205 528 1455 1842 Fragments arranged by size: 1842 1455 1205 627 528 325 217 BanII G_rGCy'C Cuts at: 1391 3435 3480 3518 1391 Size: 2044 45 38 4072 Fragments arranged by size: 4072 2044 45 38 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2755 3339 928 Size: 971 856 584 3788 Fragments arranged by size: 3788 971 856 584 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4390 4424 1903 Size: 34 2453 34 3678 Fragments arranged by size: 3678 2453 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4091 5301 4091 Size: 1210 4989 BclI T'GATC_A Cuts at: 1548 1548 Size: 6199 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4810 136 Size: 107 122 71 631 605 3138 1525 Fragments arranged by size: 3138 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6194 6194 Size: 6199 BlpI GC'TnA_GC Cuts at: 3616 3616 Size: 6199 Bme1580I G_kGCm'C Cuts at: 1790 2964 3425 3480 4260 5416 1790 Size: 1174 461 55 780 1156 2573 Fragments arranged by size: 2573 1174 1156 780 461 55 BmgBI CAC'GTC Cuts at: 1318 2668 1318 Size: 1350 4849 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2589 3930 4888 Size: 416 53 83 399 24 1172 1341 958 Cuts at: 4888 442 Size: 1753 Fragments arranged by size: 1753 1341 1172 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6199 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3186 4859 1239 Size: 1947 1673 2579 Fragments arranged by size: 2579 1947 1673 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2495 4324 5189 5487 2495 Size: 1829 865 298 3207 Fragments arranged by size: 3207 1829 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4862 882 Size: 617 3363 2219 Fragments arranged by size: 3363 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2662 3364 3990 493 Size: 711 1458 702 626 2702 Fragments arranged by size: 2702 1458 711 702 626 BsaBI GATnn'nnATC Cuts at: 6193 6193 Size: 6199 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2564 4006 4388 5023 Size: 53 83 186 936 1031 1442 382 635 Cuts at: 5023 275 Size: 1451 Fragments arranged by size: 1451 1442 1031 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2409 4632 5030 5304 5451 5862 2409 Size: 2223 398 274 147 411 2746 Fragments arranged by size: 2746 2223 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5974 Fragments arranged by size: 5974 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2143 1186 Size: 957 5242 BseYI C'CCAG_C Cuts at: 2140 2193 2654 2975 3089 3309 3489 5402 5898 Size: 53 461 321 114 220 180 1913 496 Cuts at: 5898 2140 Size: 2441 Fragments arranged by size: 2441 1913 496 461 321 220 180 114 53 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2488 3002 3296 1163 Size: 573 752 514 294 4066 Fragments arranged by size: 4066 752 573 514 294 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3649 4410 5438 1540 Size: 275 186 1648 761 1028 2301 Fragments arranged by size: 2301 1648 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2739 2955 2964 4260 4345 5416 2739 Size: 216 9 1296 85 1071 3522 Fragments arranged by size: 3522 1296 1071 216 85 9 BsmI GAATG_Cn' Cuts at: 3710 3803 3710 Size: 93 6106 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2739 2955 2964 3425 3435 3480 3518 Size: 399 949 216 9 461 10 45 38 Cuts at: 3518 4260 4345 5416 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 949 742 461 399 216 85 45 38 10 9 BspEI T'CCGG_A Cuts at: 5862 5862 Size: 6199 BspHI T'CATG_A Cuts at: 2226 2559 3042 4088 5818 2226 Size: 333 483 1046 1730 2607 Fragments arranged by size: 2607 1730 1046 483 333 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2778 3554 844 Size: 1934 776 3489 Fragments arranged by size: 3489 1934 776 BsrBI CCG'CTC Cuts at: 1394 1823 2520 2970 4086 1394 Size: 429 697 450 1116 3507 Fragments arranged by size: 3507 1116 697 450 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4694 4868 65 Size: 1183 3446 174 1396 Fragments arranged by size: 3446 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3206 3381 4843 5030 1700 Size: 110 1396 175 1462 187 2869 Fragments arranged by size: 2869 1462 1396 187 175 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6199 BssHII G'CGCG_C Cuts at: 1925 2011 2013 1925 Size: 86 2 6111 Fragments arranged by size: 6111 86 2 BssSI C'ACGA_G Cuts at: 4259 5271 4259 Size: 1012 5187 BstAPI GCAn_nnn'nTGC Cuts at: 794 3025 3099 794 Size: 2231 74 3894 Fragments arranged by size: 3894 2231 74 BstBI TT'CG_AA Cuts at: 5003 5003 Size: 6199 BstEII G'GTnAC_C Cuts at: 1184 2920 1184 Size: 1736 4463 BstXI CCAn_nnnn'nTGG Cuts at: 2662 2749 2917 2662 Size: 87 168 5944 Fragments arranged by size: 5944 168 87 BstYI r'GATC_y Cuts at: 1972 2316 3529 3896 4281 4298 5750 5836 5848 Size: 344 1213 367 385 17 1452 86 12 Cuts at: 5848 6194 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1213 385 367 346 344 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2312 2312 Size: 6199 BtgI C'CryG_G Cuts at: 513 1065 2910 2988 513 Size: 552 1845 78 3724 Fragments arranged by size: 3724 1845 552 78 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3289 487 Size: 683 56 2063 3397 Fragments arranged by size: 3397 2063 683 56 BtsI GCAGTG_nn' Cuts at: 3715 4509 4529 3715 Size: 794 20 5385 Fragments arranged by size: 5385 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6199 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6164 DraI TTT'AAA Cuts at: 3503 3503 Size: 6199 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3341 5206 809 Size: 507 2025 1865 1802 Fragments arranged by size: 2025 1865 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2073 2751 3072 Size: 54 1425 50 275 18 243 678 321 Cuts at: 3072 3646 4535 6164 8 Size: 574 889 1629 43 Fragments arranged by size: 1629 1425 889 678 574 321 275 243 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3646 1537 Size: 275 1834 4090 Fragments arranged by size: 4090 1834 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3044 3440 3455 4129 1353 Size: 1691 396 15 674 3423 Fragments arranged by size: 3423 1691 674 396 15 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2126 4786 5160 5306 387 Size: 1064 138 537 2660 374 146 1280 Fragments arranged by size: 2660 1280 1064 537 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3186 4262 4859 5646 5914 1239 Size: 393 1554 1076 597 787 268 1524 Fragments arranged by size: 1554 1524 1076 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 3256 3256 Size: 6199 EcoO109I rG'GnC_Cy Cuts at: 2373 3476 3477 2373 Size: 1103 1 5095 Fragments arranged by size: 5095 1103 1 EcoRI G'AATT_C Cuts at: 3508 3886 3508 Size: 378 5821 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3028 3028 Size: 6199 FspI TGC'GCA Cuts at: 4705 4705 Size: 6199 HaeII r_GCGC'y Cuts at: 1678 2460 3010 3052 5346 1678 Size: 782 550 42 2294 2531 Fragments arranged by size: 2531 2294 782 550 42 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2208 2240 4840 4872 4914 Size: 32 135 32 636 32 2600 32 42 Cuts at: 4914 4946 1373 Size: 32 2626 Fragments arranged by size: 2626 2600 636 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3543 3789 669 Size: 988 281 1605 246 3079 Fragments arranged by size: 3079 1605 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6199 HpaI GTT'AAC Cuts at: 3789 3789 Size: 6199 KpnI G_GTAC'C Cuts at: 3524 3524 Size: 6199 MfeI C'AATT_G Cuts at: 3798 3798 Size: 6199 MluI A'CGCG_T Cuts at: 6075 6075 Size: 6199 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2439 2802 3168 3289 5313 5497 1305 Size: 1134 363 366 121 2024 184 2007 Fragments arranged by size: 2024 2007 1134 366 363 184 121 MscI TGG'CCA Cuts at: 10 64 1489 1832 2075 2753 10 Size: 54 1425 343 243 678 3456 Fragments arranged by size: 3456 1425 678 343 243 54 MslI CAynn'nnrTG Cuts at: 518 1166 2810 2912 3407 4157 4516 4675 518 Size: 648 1644 102 495 750 359 159 2042 Fragments arranged by size: 2042 1644 750 648 495 359 159 102 MspA1I CmG'CkG Cuts at: 1606 3615 4292 5440 5685 1606 Size: 2009 677 1148 245 2120 Fragments arranged by size: 2120 2009 1148 677 245 NaeI GCC'GGC Cuts at: 3208 3208 Size: 6199 NcoI C'CATG_G Cuts at: 513 1065 2910 2988 513 Size: 552 1845 78 3724 Fragments arranged by size: 3724 1845 552 78 NdeI CA'TA_TG Cuts at: 387 387 Size: 6199 NgoMIV G'CCGG_C Cuts at: 3206 3206 Size: 6199 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6199 NotI GC'GGCC_GC Cuts at: 3646 3646 Size: 6199 NspI r_CATG'y Cuts at: 1132 1229 3557 5102 1132 Size: 97 2328 1545 2229 Fragments arranged by size: 2328 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2624 2997 3231 1348 Size: 1276 373 234 4316 Fragments arranged by size: 4316 1276 373 234 PciI A'CATG_T Cuts at: 5098 5098 Size: 6199 PflMI CCAn_nnn'nTGG Cuts at: 1108 2241 2989 1108 Size: 1133 748 4318 Fragments arranged by size: 4318 1133 748 PfoI T'CCnGG_A Cuts at: 2522 2522 Size: 6199 PmeI GTTT'AAAC Cuts at: 3503 3503 Size: 6199 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3167 3199 4268 4300 5781 5813 3167 Size: 32 1069 32 1481 32 3553 Fragments arranged by size: 3553 1481 1069 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2373 2373 Size: 6199 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6199 PsiI TTA'TAA Cuts at: 3769 3769 Size: 6199 PspOMI G'GGCC_C Cuts at: 3476 3476 Size: 6199 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2888 2920 3149 3181 2888 Size: 32 229 32 5906 Fragments arranged by size: 5906 229 32 32 PstI C_TGCA'G Cuts at: 830 2181 3190 3551 830 Size: 1351 1009 361 3478 Fragments arranged by size: 3478 1351 1009 361 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6199 SalI G'TCGA_C Cuts at: 1936 3541 1936 Size: 1605 4594 SapI GCTCTTCn'nnn_ Cuts at: 3440 3440 Size: 6199 SbfI CC_TGCA'GG Cuts at: 3551 3551 Size: 6199 ScaI AGT'ACT Cuts at: 2480 4447 6159 2480 Size: 1967 1712 2520 Fragments arranged by size: 2520 1967 1712 SfcI C'TryA_G Cuts at: 826 1045 2177 3186 3547 4682 5363 5554 826 Size: 219 1132 1009 361 1135 681 191 1471 Fragments arranged by size: 1471 1135 1132 1009 681 361 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6199 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6199 SmaI CCC'GGG Cuts at: 1872 3526 1872 Size: 1654 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2510 4303 5204 5466 820 Size: 197 926 567 1793 901 262 1553 Fragments arranged by size: 1793 1553 926 901 567 262 197 SnaBI TAC'GTA Cuts at: 493 3990 493 Size: 3497 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6199 SphI G_CATG'C Cuts at: 1132 3557 1132 Size: 2425 3774 SspI AAT'ATT Cuts at: 5 52 4123 5 Size: 47 4071 2081 Fragments arranged by size: 4071 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6199 StyI C'CwwG_G Cuts at: 513 1065 2910 2988 3638 513 Size: 552 1845 78 650 3074 Fragments arranged by size: 3074 1845 650 552 78 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2878 2996 3385 4226 4243 4396 4581 Size: 1255 1298 118 389 841 17 153 185 Cuts at: 4581 5026 325 Size: 445 1498 Fragments arranged by size: 1498 1298 1255 841 445 389 185 153 118 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2478 3165 4445 Size: 274 80 33 51 1443 501 687 1280 Cuts at: 4445 6157 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1280 687 501 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6159 6191 6159 Size: 32 6167 Tth111I GACn'n_nGTC Cuts at: 1316 2552 1316 Size: 1236 4963 XbaI T'CTAG_A Cuts at: 3535 3535 Size: 6199 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2575 2575 Size: 6199 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6199 XmaI C'CCGG_G Cuts at: 1870 3524 1870 Size: 1654 4545 XmnI GAAnn'nnTTC Cuts at: 4328 4328 Size: 6199 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4007 276 Size: 53 83 186 936 2473 2468 Fragments arranged by size: 2473 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI