Restriction map for
pFN21AE2393
(Linear) (Six-base) MAPSORT of: KIEE2393.seq Check: 6578 from: 1 to: 1494
KIEE2393.orf
With 182 enzymes: SgfI *
January 26, 2012 13:39 ..
AccI GT'mk_AC
Cuts at: 0 386 1494
Size: 386 1108
AfeI AGC'GCT
Cuts at: 0 1003 1494
Size: 1003 491
AleI CACnn'nnGTG
Cuts at: 0 907 1494
Size: 907 587
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 349 823 1494
Size: 349 474 671
Fragments arranged by size:
671 474 349
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1366 1398 1494
Size: 1366 32 96
Fragments arranged by size:
1366 96 32
AlwNI CAG_nnn'CTG
Cuts at: 0 941 1465 1494
Size: 941 524 29
Fragments arranged by size:
941 524 29
ApaI G_GGCC'C
Cuts at: 0 1475 1494
Size: 1475 19
ApaLI G'TGCA_C
Cuts at: 0 955 1494
Size: 955 539
AvaI C'yCGr_G
Cuts at: 0 1108 1494
Size: 1108 386
BamHI G'GATC_C
Cuts at: 0 311 1494
Size: 311 1183
BanI G'GyrC_C
Cuts at: 0 987 1494
Size: 987 507
BanII G_rGCy'C
Cuts at: 0 1430 1475 1494
Size: 1430 45 19
Fragments arranged by size:
1430 45 19
BbsI GAAGACnn'nnnn_
Cuts at: 0 750 1334 1494
Size: 750 584 160
Fragments arranged by size:
750 584 160
Bme1580I G_kGCm'C
Cuts at: 0 959 1420 1475 1494
Size: 959 461 55 19
Fragments arranged by size:
959 461 55 19
BmgBI CAC'GTC
Cuts at: 0 663 1494
Size: 663 831
BmrI ACTGGGnnnn_n'
Cuts at: 0 584 1494
Size: 584 910
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1181 1494
Size: 1181 313
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 490 1494
Size: 490 1004
BsaAI yAC'GTr
Cuts at: 0 657 1359 1494
Size: 657 702 135
Fragments arranged by size:
702 657 135
BsaHI Gr'CG_yC
Cuts at: 0 559 1494
Size: 559 935
BsaWI w'CCGG_w
Cuts at: 0 404 1494
Size: 404 1090
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 138 1494
Size: 138 1356
BseYI C'CCAG_C
Cuts at: 0 135 188 649 970 1084 1304 1484 1494
Size: 135 53 461 321 114 220 180 10
Fragments arranged by size:
461 321 220 180 135 114 53 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 483 997 1291 1494
Size: 483 514 294 203
Fragments arranged by size:
514 483 294 203
BsiHKAI G_wGCw'C
Cuts at: 0 734 950 959 1494
Size: 734 216 9 535
Fragments arranged by size:
734 535 216 9
Bsp1286I G_dGCh'C
Cuts at: 0 734 950 959 1420 1430 1475 1494
Size: 734 216 9 461 10 45 19
Fragments arranged by size:
734 461 216 45 19 10 9
BspHI T'CATG_A
Cuts at: 0 221 554 1037 1494
Size: 221 333 483 457
Fragments arranged by size:
483 457 333 221
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 773 1494
Size: 773 721
BsrBI CCG'CTC
Cuts at: 0 515 965 1494
Size: 515 450 529
Fragments arranged by size:
529 515 450
BsrFI r'CCGG_y
Cuts at: 0 1201 1376 1494
Size: 1201 175 118
Fragments arranged by size:
1201 175 118
BssHII G'CGCG_C
Cuts at: 0 6 8 1494
Size: 6 2 1486
Fragments arranged by size:
1486 6 2
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1020 1094 1494
Size: 1020 74 400
Fragments arranged by size:
1020 400 74
BstEII G'GTnAC_C
Cuts at: 0 915 1494
Size: 915 579
BstXI CCAn_nnnn'nTGG
Cuts at: 0 657 744 912 1494
Size: 657 87 168 582
Fragments arranged by size:
657 582 168 87
BstYI r'GATC_y
Cuts at: 0 311 1494
Size: 311 1183
Bsu36I CC'TnA_GG
Cuts at: 0 307 1494
Size: 307 1187
BtgI C'CryG_G
Cuts at: 0 905 983 1494
Size: 905 78 511
Fragments arranged by size:
905 511 78
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1284 1494
Size: 1284 210
DrdI GACnn_nn'nnGTC
Cuts at: 0 1336 1494
Size: 1336 158
EaeI y'GGCC_r
Cuts at: 0 68 746 1067 1494
Size: 68 678 321 427
Fragments arranged by size:
678 427 321 68
EarI CTCTTCn'nnn_
Cuts at: 0 1039 1435 1450 1494
Size: 1039 396 15 44
Fragments arranged by size:
1039 396 44 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 121 1494
Size: 121 1373
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1181 1494
Size: 1181 313
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1251 1494
Size: 1251 243
EcoO109I rG'GnC_Cy
Cuts at: 0 368 1471 1472 1494
Size: 368 1103 1 22
Fragments arranged by size:
1103 368 22 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1023 1494
Size: 1023 471
HaeII r_GCGC'y
Cuts at: 0 455 1005 1047 1494
Size: 455 550 42 447
Fragments arranged by size:
550 455 447 42
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 203 235 1494
Size: 203 32 1259
Fragments arranged by size:
1259 203 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 434 797 1163 1284 1494
Size: 434 363 366 121 210
Fragments arranged by size:
434 366 363 210 121
MscI TGG'CCA
Cuts at: 0 70 748 1494
Size: 70 678 746
Fragments arranged by size:
746 678 70
MslI CAynn'nnrTG
Cuts at: 0 805 907 1402 1494
Size: 805 102 495 92
Fragments arranged by size:
805 495 102 92
NaeI GCC'GGC
Cuts at: 0 1203 1494
Size: 1203 291
NcoI C'CATG_G
Cuts at: 0 905 983 1494
Size: 905 78 511
Fragments arranged by size:
905 511 78
NgoMIV G'CCGG_C
Cuts at: 0 1201 1494
Size: 1201 293
PasI CC'CwG_GG
Cuts at: 0 619 992 1226 1494
Size: 619 373 234 268
Fragments arranged by size:
619 373 268 234
PflMI CCAn_nnn'nTGG
Cuts at: 0 236 984 1494
Size: 236 748 510
Fragments arranged by size:
748 510 236
PfoI T'CCnGG_A
Cuts at: 0 517 1494
Size: 517 977
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1162 1194 1494
Size: 1162 32 300
Fragments arranged by size:
1162 300 32
PpuMI rG'GwC_Cy
Cuts at: 0 368 1494
Size: 368 1126
PspOMI G'GGCC_C
Cuts at: 0 1471 1494
Size: 1471 23
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 883 915 1144 1176 1494
Size: 883 32 229 32 318
Fragments arranged by size:
883 318 229 32 32
PstI C_TGCA'G
Cuts at: 0 176 1185 1494
Size: 176 1009 309
Fragments arranged by size:
1009 309 176
SapI GCTCTTCn'nnn_
Cuts at: 0 1435 1494
Size: 1435 59
ScaI AGT'ACT
Cuts at: 0 475 1494
Size: 475 1019
SfcI C'TryA_G
Cuts at: 0 172 1181 1494
Size: 172 1009 313
Fragments arranged by size:
1009 313 172
SmlI C'TyrA_G
Cuts at: 0 505 1494
Size: 505 989
StyI C'CwwG_G
Cuts at: 0 905 983 1494
Size: 905 78 511
Fragments arranged by size:
905 511 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 873 991 1380 1494
Size: 873 118 389 114
Fragments arranged by size:
873 389 118 114
TatI w'GTAC_w
Cuts at: 0 473 1160 1494
Size: 473 687 334
Fragments arranged by size:
687 473 334
Tth111I GACn'n_nGTC
Cuts at: 0 547 1494
Size: 547 947
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 570 1494
Size: 570 924
Enzymes that do cut:
AccI AfeI AleI AlfI AloI AlwNI ApaI ApaLI
AvaI BamHI BanI BanII BbsI Bme1580I BmgBI BmrI
BpmI BpuEI BsaAI BsaHI BsaWI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrFI BssHII BstAPI
BstEII BstXI BstYI Bsu36I BtgI BtgZI DrdI EaeI
EarI EciI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I
MmeI MscI MslI NaeI NcoI NgoMIV PasI PflMI
PfoI PpiI PpuMI PspOMI PsrI PstI SapI ScaI
SfcI SmlI StyI TaqII TatI Tth111I XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AflII AflIII AgeI
AhdI ApoI AscI AseI AsiSI AvrII BaeI BbeI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
BmtI BplI Bpu10I BsaI BsaBI BsaXI BsiEI BsiWI
BsmI BsmBI BspEI BsrDI BsrGI BssSI BstBI BstZ17I
BtsI ClaI CspCI DraI DraIII EagI EcoICRI EcoRI
EcoRV FseI FspI FspAI HincII HindIII HpaI KasI
KpnI MfeI MluI MspA1I NarI NdeI NheI NotI
NruI NsiI NspI PacI PciI PmeI PmlI PshAI
PsiI PspXI PvuI PvuII RsrII SacI SacII SalI
SanDI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI TstI
XbaI XhoI XmaI XmnI ZraI