Restriction map for
pFN21AE5652
(Circular) (Six-base) MAPSORT of: pFN21AE5652.seq Check: 5245 from: 1 to: 6220
pFN21AE5652 6220 bp
With 182 enzymes: SgfI *
December 8, 2014 13:56 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4030 278
Size: 53 83 186 936 2494 2468
Fragments arranged by size:
2494 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3563 1937
Size: 1626 4594
Acc65I G'GTAC_C
Cuts at: 3541 3541
Size: 6220
AclI AA'CG_TT
Cuts at: 1563 4347 4720 1563
Size: 2784 373 3063
Fragments arranged by size:
3063 2784 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2312 3136 4283 5667 5935 1632
Size: 680 824 1147 1384 268 1917
Fragments arranged by size:
1917 1384 1147 824 680 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6023
AflIII A'CryG_T
Cuts at: 1203 2169 5119 6096 1203
Size: 966 2950 977 1327
Fragments arranged by size:
2950 1327 977 966
AgeI A'CCGG_T
Cuts at: 5051 5051
Size: 6220
AhdI GACnn_n'nnGTC
Cuts at: 4949 4949
Size: 6220
AleI CACnn'nnGTG
Cuts at: 1166 3458 1166
Size: 2292 3928
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6220
AlwNI CAG_nnn'CTG
Cuts at: 2105 3963 5535 2105
Size: 1858 1572 2790
Fragments arranged by size:
2790 1858 1572
ApaLI G'TGCA_C
Cuts at: 2987 4277 5433 2987
Size: 1290 1156 3774
Fragments arranged by size:
3774 1290 1156
ApoI r'AATT_y
Cuts at: 1493 3529 3755 3907 6016 1493
Size: 2036 226 152 2109 1697
Fragments arranged by size:
2109 2036 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4774 160
Size: 872 3742 1606
Fragments arranged by size:
3742 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6220
AvaI C'yCGr_G
Cuts at: 1870 1943 3545 1870
Size: 73 1602 4545
Fragments arranged by size:
4545 1602 73
BamHI G'GATC_C
Cuts at: 3550 3917 3550
Size: 367 5853
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2718 3125 3541 4996 618
Size: 325 217 627 931 407 416 1455 1842
Fragments arranged by size:
1842 1455 931 627 416 407 325 217
BanII G_rGCy'C
Cuts at: 1391 2012 2477 3135 3539 1391
Size: 621 465 658 404 4072
Fragments arranged by size:
4072 658 621 465 404
BbeI G_GCGC'C
Cuts at: 2722 2722
Size: 6220
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2782 2965 3038 928
Size: 971 883 183 73 4110
Fragments arranged by size:
4110 971 883 183 73
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4411 4445 1903
Size: 34 2474 34 3678
Fragments arranged by size:
3678 2474 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2445 4112 5322 2445
Size: 1667 1210 3343
Fragments arranged by size:
3343 1667 1210
BclI T'GATC_A
Cuts at: 1548 2871 1548
Size: 1323 4897
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2050 2102 4831
Size: 107 122 71 631 605 378 52 2729
Cuts at: 4831 136
Size: 1525
Fragments arranged by size:
2729 1525 631 605 378 122 107 71
52
BglII A'GATC_T
Cuts at: 2349 2682 6215 2349
Size: 333 3533 2354
Fragments arranged by size:
3533 2354 333
BlpI GC'TnA_GC
Cuts at: 2106 3637 2106
Size: 1531 4689
Bme1580I G_kGCm'C
Cuts at: 1790 2991 4281 5437 1790
Size: 1201 1290 1156 2573
Fragments arranged by size:
2573 1290 1201 1156
BmgBI CAC'GTC
Cuts at: 1318 2491 1318
Size: 1173 5047
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2174 2445 3951
Size: 416 53 83 399 24 757 271 1506
Cuts at: 3951 4909 442
Size: 958 1753
Fragments arranged by size:
1753 1506 958 757 416 399 271 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6220
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2942 2942
Size: 6220
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4880 1239
Size: 3641 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2249 2411 2582 4345 5210 5508 2249
Size: 162 171 1763 865 298 2961
Fragments arranged by size:
2961 1763 865 298 171 162
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2243 2873 4883 882
Size: 617 744 630 2010 2219
Fragments arranged by size:
2219 2010 744 630 617
BsaAI yAC'GTr
Cuts at: 493 1204 4011 493
Size: 711 2807 2702
Fragments arranged by size:
2807 2702 711
BsaBI GATnn'nnATC
Cuts at: 3236 6214 3236
Size: 2978 3242
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2719 4027 4409 5044
Size: 53 83 186 936 1186 1308 382 635
Cuts at: 5044 275
Size: 1451
Fragments arranged by size:
1451 1308 1186 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4653 5051 5325 5472 5883 4653
Size: 398 274 147 411 4990
Fragments arranged by size:
4990 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2786 2816 1178
Size: 30 165 30 1383 30 4582
Fragments arranged by size:
4582 1383 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2693 2834 1186
Size: 1507 141 4572
Fragments arranged by size:
4572 1507 141
BseYI C'CCAG_C
Cuts at: 5423 5919 5423
Size: 496 5724
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2325 1163
Size: 573 589 5058
Fragments arranged by size:
5058 589 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3670 4431 5459 1540
Size: 275 186 1669 761 1028 2301
Fragments arranged by size:
2301 1669 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2112 2477 2787 2982 2991 3135 4281 4366 5437
Size: 365 310 195 9 144 1146 85 1071
Cuts at: 5437 2112
Size: 2895
Fragments arranged by size:
2895 1146 1071 365 310 195 144 85
9
BsmI GAATG_Cn'
Cuts at: 3465 3731 3824 3465
Size: 266 93 5861
Fragments arranged by size:
5861 266 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2112 2477 2787 2982 2991 3135
Size: 399 222 100 365 310 195 9 144
Cuts at: 3135 3539 4281 4366 5437 1391
Size: 404 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 404 399 365 310 222
195 144 100 85 9
BspEI T'CCGG_A
Cuts at: 5883 5883
Size: 6220
BspHI T'CATG_A
Cuts at: 3069 4109 5839 3069
Size: 1040 1730 3450
Fragments arranged by size:
3450 1730 1040
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2805 3057 3575 844
Size: 1961 252 518 3489
Fragments arranged by size:
3489 1961 518 252
BsrBI CCG'CTC
Cuts at: 1394 1823 2020 2125 2616 4107 1394
Size: 429 197 105 491 1491 3507
Fragments arranged by size:
3507 1491 491 429 197 105
BsrDI GCAATG_nn'
Cuts at: 65 1248 4715 4889 65
Size: 1183 3467 174 1396
Fragments arranged by size:
3467 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4864 5051 1700
Size: 110 3054 187 2869
Fragments arranged by size:
3054 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2709 96
Size: 2613 3607
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6220
BssSI C'ACGA_G
Cuts at: 3262 4280 5292 3262
Size: 1018 1012 4190
Fragments arranged by size:
4190 1018 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6220
BstBI TT'CG_AA
Cuts at: 5024 5024
Size: 6220
BstEII G'GTnAC_C
Cuts at: 1184 2821 1184
Size: 1637 4583
BstXI CCAn_nnnn'nTGG
Cuts at: 2899 2899
Size: 6220
BstYI r'GATC_y
Cuts at: 1972 2349 2682 3550 3917 4302 4319 5771 5857
Size: 377 333 868 367 385 17 1452 86
Cuts at: 5857 5869 6215 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 868 385 377 367 346 333
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2048 3420 513
Size: 552 939 44 1372 3313
Fragments arranged by size:
3313 1372 939 552 44
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5481
Fragments arranged by size:
5481 683 56
BtsI GCAGTG_nn'
Cuts at: 3736 4530 4550 3736
Size: 794 20 5406
Fragments arranged by size:
5406 794 20
ClaI AT'CG_AT
Cuts at: 1554 2565 1554
Size: 1011 5209
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6185
DraI TTT'AAA
Cuts at: 3524 3524
Size: 6220
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5227 809
Size: 507 3911 1802
Fragments arranged by size:
3911 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2088 2460 3504
Size: 54 1425 50 275 18 258 372 1044
Cuts at: 3504 3667 4556 6185 8
Size: 163 889 1629 43
Fragments arranged by size:
1629 1425 1044 889 372 275 258 163
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3667 1537
Size: 275 1855 4090
Fragments arranged by size:
4090 1855 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2590 4150 1353
Size: 1237 1560 3423
Fragments arranged by size:
3423 1560 1237
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4807 5181 5327 387
Size: 1064 138 3218 374 146 1280
Fragments arranged by size:
3218 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2475 3133 2475
Size: 658 5562
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2312 3136 4283 4880 5667 5935 1239
Size: 393 680 824 1147 597 787 268 1524
Fragments arranged by size:
1524 1147 824 787 680 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2094 3114 2094
Size: 1020 5200
EcoRI G'AATT_C
Cuts at: 3529 3907 3529
Size: 378 5842
FspI TGC'GCA
Cuts at: 4726 4726
Size: 6220
HaeII r_GCGC'y
Cuts at: 1678 2039 2722 5367 1678
Size: 361 683 2645 2531
Fragments arranged by size:
2645 2531 683 361
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4861 4893 4935 4967 1373
Size: 32 135 32 3289 32 42 32 2626
Fragments arranged by size:
3289 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3564 3810 669
Size: 988 281 1626 246 3079
Fragments arranged by size:
3079 1626 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6220
HpaI GTT'AAC
Cuts at: 3810 3810
Size: 6220
KasI G'GCGC_C
Cuts at: 2718 2718
Size: 6220
KpnI G_GTAC'C
Cuts at: 3545 3545
Size: 6220
MfeI C'AATT_G
Cuts at: 3819 3819
Size: 6220
MluI A'CGCG_T
Cuts at: 6096 6096
Size: 6220
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2829 5334 5518 1305
Size: 1524 2505 184 2007
Fragments arranged by size:
2505 2007 1524 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2090 3506 10
Size: 54 1425 343 258 1416 2724
Fragments arranged by size:
2724 1425 1416 343 258 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3068 3458 4178 4537 4696 518
Size: 648 1902 390 720 359 159 2042
Fragments arranged by size:
2042 1902 720 648 390 359 159
MspA1I CmG'CkG
Cuts at: 1606 2050 2215 3636 4313 5461 5706 1606
Size: 444 165 1421 677 1148 245 2120
Fragments arranged by size:
2120 1421 1148 677 444 245 165
NarI GG'CG_CC
Cuts at: 2719 2719
Size: 6220
NcoI C'CATG_G
Cuts at: 513 1065 2004 3420 513
Size: 552 939 1416 3313
Fragments arranged by size:
3313 1416 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6220
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6220
NotI GC'GGCC_GC
Cuts at: 3667 3667
Size: 6220
NspI r_CATG'y
Cuts at: 1132 1229 3578 5123 1132
Size: 97 2349 1545 2229
Fragments arranged by size:
2349 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3057 1348
Size: 1709 4511
PciI A'CATG_T
Cuts at: 5119 5119
Size: 6220
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2245 2259 1108
Size: 1137 14 5069
Fragments arranged by size:
5069 1137 14
PmeI GTTT'AAAC
Cuts at: 3524 3524
Size: 6220
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4289 4321 5802 5834 4289
Size: 32 1481 32 4675
Fragments arranged by size:
4675 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6220
PsiI TTA'TAA
Cuts at: 3790 3790
Size: 6220
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2915 2947 2915
Size: 32 6188
PstI C_TGCA'G
Cuts at: 830 3572 830
Size: 2742 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6220
PvuII CAG'CTG
Cuts at: 2215 2215
Size: 6220
SacI G_AGCT'C
Cuts at: 2477 3135 2477
Size: 658 5562
SacII CC_GC'GG
Cuts at: 2051 2051
Size: 6220
SalI G'TCGA_C
Cuts at: 1936 3562 1936
Size: 1626 4594
SapI GCTCTTCn'nnn_
Cuts at: 2590 2590
Size: 6220
SbfI CC_TGCA'GG
Cuts at: 3572 3572
Size: 6220
ScaI AGT'ACT
Cuts at: 4468 6180 4468
Size: 1712 4508
SfcI C'TryA_G
Cuts at: 826 1045 2160 2992 3568 4703 5384 5575 826
Size: 219 1115 832 576 1135 681 191 1471
Fragments arranged by size:
1471 1135 1115 832 681 576 219 191
SfoI GGC'GCC
Cuts at: 2720 2720
Size: 6220
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6220
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6220
SmaI CCC'GGG
Cuts at: 1872 3547 1872
Size: 1675 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2264 2426 2597 4324 5225 5487
Size: 197 926 321 162 171 1727 901 262
Cuts at: 5487 820
Size: 1553
Fragments arranged by size:
1727 1553 926 901 321 262 197 171
162
SnaBI TAC'GTA
Cuts at: 493 4011 493
Size: 3518 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6220
SphI G_CATG'C
Cuts at: 1132 3578 1132
Size: 2446 3774
SspI AAT'ATT
Cuts at: 5 52 4144 5
Size: 47 4092 2081
Fragments arranged by size:
4092 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6220
StyI C'CwwG_G
Cuts at: 513 1065 2004 3420 3659 513
Size: 552 939 1416 239 3074
Fragments arranged by size:
3074 1416 939 552 239
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3016 4247 4264 4417 4602 5047 325
Size: 1255 1436 1231 17 153 185 445 1498
Fragments arranged by size:
1498 1436 1255 1231 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2430 2664 2709
Size: 274 80 33 51 1443 453 234 45
Cuts at: 2709 4466 6178 96
Size: 1757 1712 138
Fragments arranged by size:
1757 1712 1443 453 274 234 138 80
51 45 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3191 3223 6180 6212 3191
Size: 32 2957 32 3199
Fragments arranged by size:
3199 2957 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2180 1316
Size: 864 5356
XbaI T'CTAG_A
Cuts at: 3556 3556
Size: 6220
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2212 2212
Size: 6220
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6220
XmaI C'CCGG_G
Cuts at: 1870 3545 1870
Size: 1675 4545
XmnI GAAnn'nnTTC
Cuts at: 3149 4349 3149
Size: 1200 5020
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4028 276
Size: 53 83 186 936 2494 2468
Fragments arranged by size:
2494 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PsrI PstI PvuI PvuII SacI SacII SalI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI
EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI
PacI PfoI PmlI PpuMI PspOMI PspXI RsrII SanDI
SexAI SfiI SrfI SwaI