(Linear) (Six-base) MAPSORT of: KIEE5652.seq Check: 3788 from: 1 to: 1515 KIEE5652.orf With 182 enzymes: SgfI * December 5, 2014 13:05 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 307 1131 1515 Size: 307 824 384 Fragments arranged by size: 824 384 307 AflIII A'CryG_T Cuts at: 0 164 1515 Size: 164 1351 AleI CACnn'nnGTG Cuts at: 0 1453 1515 Size: 1453 62 AlwNI CAG_nnn'CTG Cuts at: 0 100 1515 Size: 100 1415 ApaLI G'TGCA_C Cuts at: 0 982 1515 Size: 982 533 BanI G'GyrC_C Cuts at: 0 713 1120 1515 Size: 713 407 395 Fragments arranged by size: 713 407 395 BanII G_rGCy'C Cuts at: 0 7 472 1130 1515 Size: 7 465 658 385 Fragments arranged by size: 658 465 385 7 BbeI G_GCGC'C Cuts at: 0 717 1515 Size: 717 798 BbsI GAAGACnn'nnnn_ Cuts at: 0 777 960 1033 1515 Size: 777 183 73 482 Fragments arranged by size: 777 482 183 73 BciVI GTATCCnnnnn_n' Cuts at: 0 440 1515 Size: 440 1075 BclI T'GATC_A Cuts at: 0 866 1515 Size: 866 649 BglI GCCn_nnn'nGGC Cuts at: 0 45 97 1515 Size: 45 52 1418 Fragments arranged by size: 1418 52 45 BglII A'GATC_T Cuts at: 0 344 677 1515 Size: 344 333 838 Fragments arranged by size: 838 344 333 BlpI GC'TnA_GC Cuts at: 0 101 1515 Size: 101 1414 Bme1580I G_kGCm'C Cuts at: 0 986 1515 Size: 986 529 BmgBI CAC'GTC Cuts at: 0 486 1515 Size: 486 1029 BmrI ACTGGGnnnn_n' Cuts at: 0 169 440 1515 Size: 169 271 1075 Fragments arranged by size: 1075 271 169 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 937 1515 Size: 937 578 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 244 406 577 1515 Size: 244 162 171 938 Fragments arranged by size: 938 244 171 162 BsaI GGTCTCn'nnnn_ Cuts at: 0 238 868 1515 Size: 238 630 647 Fragments arranged by size: 647 630 238 BsaBI GATnn'nnATC Cuts at: 0 1231 1515 Size: 1231 284 BsaHI Gr'CG_yC Cuts at: 0 714 1515 Size: 714 801 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 781 811 1515 Size: 781 30 704 Fragments arranged by size: 781 704 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 688 829 1515 Size: 688 141 686 Fragments arranged by size: 688 686 141 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 320 1515 Size: 320 1195 BsiHKAI G_wGCw'C Cuts at: 0 107 472 782 977 986 1130 1515 Size: 107 365 310 195 9 144 385 Fragments arranged by size: 385 365 310 195 144 107 9 BsmI GAATG_Cn' Cuts at: 0 1460 1515 Size: 1460 55 Bsp1286I G_dGCh'C Cuts at: 0 7 107 472 782 977 986 1130 1515 Size: 7 100 365 310 195 9 144 385 Fragments arranged by size: 385 365 310 195 144 100 9 7 BspHI T'CATG_A Cuts at: 0 1064 1515 Size: 1064 451 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 800 1052 1515 Size: 800 252 463 Fragments arranged by size: 800 463 252 BsrBI CCG'CTC Cuts at: 0 15 120 611 1515 Size: 15 105 491 904 Fragments arranged by size: 904 491 105 15 BsrGI T'GTAC_A Cuts at: 0 704 1515 Size: 704 811 BssSI C'ACGA_G Cuts at: 0 1257 1515 Size: 1257 258 BstEII G'GTnAC_C Cuts at: 0 816 1515 Size: 816 699 BstXI CCAn_nnnn'nTGG Cuts at: 0 894 1515 Size: 894 621 BstYI r'GATC_y Cuts at: 0 344 677 1515 Size: 344 333 838 Fragments arranged by size: 838 344 333 BtgI C'CryG_G Cuts at: 0 43 1415 1515 Size: 43 1372 100 Fragments arranged by size: 1372 100 43 ClaI AT'CG_AT Cuts at: 0 560 1515 Size: 560 955 EaeI y'GGCC_r Cuts at: 0 83 455 1499 1515 Size: 83 372 1044 16 Fragments arranged by size: 1044 372 83 16 EarI CTCTTCn'nnn_ Cuts at: 0 585 1515 Size: 585 930 EcoICRI GAG'CTC Cuts at: 0 470 1128 1515 Size: 470 658 387 Fragments arranged by size: 658 470 387 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 307 1131 1515 Size: 307 824 384 Fragments arranged by size: 824 384 307 EcoO109I rG'GnC_Cy Cuts at: 0 89 1109 1515 Size: 89 1020 406 Fragments arranged by size: 1020 406 89 HaeII r_GCGC'y Cuts at: 0 34 717 1515 Size: 34 683 798 Fragments arranged by size: 798 683 34 KasI G'GCGC_C Cuts at: 0 713 1515 Size: 713 802 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 824 1515 Size: 824 691 MscI TGG'CCA Cuts at: 0 85 1501 1515 Size: 85 1416 14 Fragments arranged by size: 1416 85 14 MslI CAynn'nnrTG Cuts at: 0 1063 1453 1515 Size: 1063 390 62 Fragments arranged by size: 1063 390 62 MspA1I CmG'CkG Cuts at: 0 45 210 1515 Size: 45 165 1305 Fragments arranged by size: 1305 165 45 NarI GG'CG_CC Cuts at: 0 714 1515 Size: 714 801 NcoI C'CATG_G Cuts at: 0 1415 1515 Size: 1415 100 PasI CC'CwG_GG Cuts at: 0 1052 1515 Size: 1052 463 PflMI CCAn_nnn'nTGG Cuts at: 0 240 254 1515 Size: 240 14 1261 Fragments arranged by size: 1261 240 14 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 910 942 1515 Size: 910 32 573 Fragments arranged by size: 910 573 32 PvuII CAG'CTG Cuts at: 0 210 1515 Size: 210 1305 SacI G_AGCT'C Cuts at: 0 472 1130 1515 Size: 472 658 385 Fragments arranged by size: 658 472 385 SacII CC_GC'GG Cuts at: 0 46 1515 Size: 46 1469 SapI GCTCTTCn'nnn_ Cuts at: 0 585 1515 Size: 585 930 SfcI C'TryA_G Cuts at: 0 155 987 1515 Size: 155 832 528 Fragments arranged by size: 832 528 155 SfoI GGC'GCC Cuts at: 0 715 1515 Size: 715 800 SmlI C'TyrA_G Cuts at: 0 259 421 592 1515 Size: 259 162 171 923 Fragments arranged by size: 923 259 171 162 StyI C'CwwG_G Cuts at: 0 1415 1515 Size: 1415 100 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1011 1515 Size: 1011 504 TatI w'GTAC_w Cuts at: 0 425 659 704 1515 Size: 425 234 45 811 Fragments arranged by size: 811 425 234 45 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1186 1218 1515 Size: 1186 32 297 Fragments arranged by size: 1186 297 32 Tth111I GACn'n_nGTC Cuts at: 0 175 1515 Size: 175 1340 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 207 1515 Size: 207 1308 XmnI GAAnn'nnTTC Cuts at: 0 1144 1515 Size: 1144 371 Enzymes that do cut: AcuI AflIII AleI AlwNI ApaLI BanI BanII BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpuEI BsaI BsaBI BsaHI BsaXI BseRI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrGI BssSI BstEII BstXI BstYI BtgI ClaI EaeI EarI EcoICRI Eco57MI EcoO109I HaeII KasI MmeI MscI MslI MspA1I NarI NcoI PasI PflMI PsrI PvuII SacI SacII SapI SfcI SfoI SmlI StyI TaqII TatI TstI Tth111I XcmI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AlfI AloI ApaI ApoI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbvCI BcgI BfrBI BmtI BpmI Bpu10I BsaAI BsaWI BseYI BsiEI BsiWI BsmBI BspEI BsrDI BsrFI BssHII BstAPI BstBI BstZ17I Bsu36I BtgZI BtsI CspCI DraI DraIII DrdI EagI EciI EcoNI EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI RsrII SalI SanDI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI XbaI XhoI XmaI ZraI