(Linear) (Six-base) MAPSORT of: KIEE5077.seq Check: 1904 from: 1 to: 597 KIEE5077.orf With 182 enzymes: SgfI * September 8, 2014 17:51 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 183 597 Size: 183 414 AflII C'TTAA_G Cuts at: 0 385 597 Size: 385 212 AlwNI CAG_nnn'CTG Cuts at: 0 206 597 Size: 206 391 ApaLI G'TGCA_C Cuts at: 0 106 597 Size: 106 491 AvaI C'yCGr_G Cuts at: 0 66 562 597 Size: 66 496 35 Fragments arranged by size: 496 66 35 AvrII C'CTAG_G Cuts at: 0 516 597 Size: 516 81 BamHI G'GATC_C Cuts at: 0 432 597 Size: 432 165 BanI G'GyrC_C Cuts at: 0 33 93 597 Size: 33 60 504 Fragments arranged by size: 504 60 33 BanII G_rGCy'C Cuts at: 0 73 597 Size: 73 524 BbeI G_GCGC'C Cuts at: 0 37 97 597 Size: 37 60 500 Fragments arranged by size: 500 60 37 BbsI GAAGACnn'nnnn_ Cuts at: 0 189 597 Size: 189 408 BglI GCCn_nnn'nGGC Cuts at: 0 169 266 597 Size: 169 97 331 Fragments arranged by size: 331 169 97 Bme1580I G_kGCm'C Cuts at: 0 110 398 597 Size: 110 288 199 Fragments arranged by size: 288 199 110 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 554 597 Size: 554 43 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 235 597 Size: 235 362 BsaAI yAC'GTr Cuts at: 0 236 597 Size: 236 361 BsaHI Gr'CG_yC Cuts at: 0 34 94 352 597 Size: 34 60 258 245 Fragments arranged by size: 258 245 60 34 BsaWI w'CCGG_w Cuts at: 0 137 296 597 Size: 137 159 301 Fragments arranged by size: 301 159 137 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 525 597 Size: 525 72 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 256 597 Size: 256 341 BsiEI CG_ry'CG Cuts at: 0 213 368 597 Size: 213 155 229 Fragments arranged by size: 229 213 155 BsiHKAI G_wGCw'C Cuts at: 0 110 597 Size: 110 487 BsiWI C'GTAC_G Cuts at: 0 303 597 Size: 303 294 Bsp1286I G_dGCh'C Cuts at: 0 73 110 398 597 Size: 73 37 288 199 Fragments arranged by size: 288 199 73 37 BspEI T'CCGG_A Cuts at: 0 137 597 Size: 137 460 BsrFI r'CCGG_y Cuts at: 0 363 597 Size: 363 234 BssHII G'CGCG_C Cuts at: 0 186 597 Size: 186 411 BstYI r'GATC_y Cuts at: 0 134 245 432 597 Size: 134 111 187 165 Fragments arranged by size: 187 165 134 111 EaeI y'GGCC_r Cuts at: 0 210 365 597 Size: 210 155 232 Fragments arranged by size: 232 210 155 EagI C'GGCC_G Cuts at: 0 210 365 597 Size: 210 155 232 Fragments arranged by size: 232 210 155 EarI CTCTTCn'nnn_ Cuts at: 0 23 597 Size: 23 574 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 183 554 597 Size: 183 371 43 Fragments arranged by size: 371 183 43 EcoNI CCTnn'n_nnAGG Cuts at: 0 274 597 Size: 274 323 HaeII r_GCGC'y Cuts at: 0 37 97 258 597 Size: 37 60 161 339 Fragments arranged by size: 339 161 60 37 KasI G'GCGC_C Cuts at: 0 33 93 597 Size: 33 60 504 Fragments arranged by size: 504 60 33 MspA1I CmG'CkG Cuts at: 0 40 203 532 597 Size: 40 163 329 65 Fragments arranged by size: 329 163 65 40 NaeI GCC'GGC Cuts at: 0 365 597 Size: 365 232 NarI GG'CG_CC Cuts at: 0 34 94 597 Size: 34 60 503 Fragments arranged by size: 503 60 34 NgoMIV G'CCGG_C Cuts at: 0 363 597 Size: 363 234 NspI r_CATG'y Cuts at: 0 114 597 Size: 114 483 PmlI CAC'GTG Cuts at: 0 236 597 Size: 236 361 PvuII CAG'CTG Cuts at: 0 532 597 Size: 532 65 SexAI A'CCwGG_T Cuts at: 0 275 597 Size: 275 322 SfoI GGC'GCC Cuts at: 0 35 95 597 Size: 35 60 502 Fragments arranged by size: 502 60 35 SmaI CCC'GGG Cuts at: 0 68 597 Size: 68 529 SmlI C'TyrA_G Cuts at: 0 250 385 597 Size: 250 135 212 Fragments arranged by size: 250 212 135 SrfI GCCC'GGGC Cuts at: 0 68 597 Size: 68 529 StyI C'CwwG_G Cuts at: 0 419 516 597 Size: 419 97 81 Fragments arranged by size: 419 97 81 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 165 426 597 Size: 165 261 171 Fragments arranged by size: 261 171 165 XmaI C'CCGG_G Cuts at: 0 66 597 Size: 66 531 Enzymes that do cut: AcuI AflII AlwNI ApaLI AvaI AvrII BamHI BanI BanII BbeI BbsI BglI Bme1580I BpmI BpuEI BsaAI BsaHI BsaWI BseRI BsgI BsiEI BsiHKAI BsiWI Bsp1286I BspEI BsrFI BssHII BstYI EaeI EagI EarI Eco57MI EcoNI HaeII KasI MspA1I NaeI NarI NgoMIV NspI PmlI PvuII SexAI SfoI SmaI SmlI SrfI StyI XcmI XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflIII AgeI AhdI AleI AlfI AloI ApaI ApoI AscI AseI AsiSI BaeI BbvCI BcgI BciVI BclI BfrBI BglII BlpI BmgBI BmrI BmtI BplI Bpu10I BsaI BsaBI BsaXI BseYI BsmI BsmBI BspHI BspMI BsrBI BsrDI BsrGI BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EciI EcoICRI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI NcoI NdeI NheI NotI NruI NsiI PacI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SnaBI SpeI SphI SspI StuI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmnI ZraI