################################## # NO CUT ENZYMES ( 138 enzymes) # ################################## Acc65I AccIII AclI AgeI AhdI Alw44I AlwI AlwNI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaII AviII AvrII BalI BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BcnI BglI BlnI BsaAI BsaBI BsaI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsmFI BsmI Bsp1407I BspDI BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Cfr10I Cfr13I ClaI CpoI DraII DraIII EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRI EcoT22I FseI FspI HaeII HincII HindII HindIII HpaI KasI KpnI KspI MamI MluI MluNI MroI MscI NaeI NarI NciI NdeI NgoMIV NlaIV NotI NruI NsiI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacII SalI Sau96I SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XmaCI XmaI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AatII 1000 GACGTC GACGT^C AccI 1257 GTCTAC GT^[AC][GT]AC AccII 343 CGCG CG^CG AflII 1263 CTTAAG C^TTAAG Asp700I 40 GAAATTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 715 GAGCTC G[AT]GC[AT]^C AvaI 251 CTCGAG C^[CT]CG[GA]G BanII 715 GAGCTC G[GA]GC[CT]^C BclI 885 TGATCA T^GATCA BfrI 1263 CTTAAG C^TTAAG BglII 56 AGATCT A^GATCT BlpI 370 GCTGAGC GC^T[ACGT]AGC Bpu1102I 370 GCTGAGC GC^T[ACGT]AGC BsaJI 641 CCATGG C^C[ACGT][ACGT]GG BsaWI 323 ACCGGA [AT]^CCGG[AT] BsiHKAI 715 GAGCTC G[AT]GC[AT]^C BsoBI 251 CTCGAG C^[CT]CG[GA]G BspLU11I 100 ACATGT A^CATGT BstUI 343 CGCG CG^CG BstYI 56 AGATCT [GA]^GATC[CT] CelII 370 GCTGAGC GC^T[ACGT]AGC CfoI 342 GCGC GCG^C DraI 1306 TTTAAA TTT^AAA DrdI 478 GACTCCGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 641 CCATGG C^C[GA][CT]GG EcoRV 538 GATATC GAT^ATC EcoT14I 641 CCATGG C^C[AT][AT]GG FauI 780 GCGGG CCCGC(4/6) FbaI 885 TGATCA T^GATCA HapII 324 CCGG C^CGG HgaI 381 GACGC GACGC(5/10) HhaI 342 GCGC GCG^C HinP1I 342 GCGC G^CGC HpaII 324 CCGG C^CGG MfeI 796 CAATTG C^AATTG MflI 56 AGATCT [GA]^GATC[CT] MslI 85 CATTATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 324 CCGG C^CGG MunI 796 CAATTG C^AATTG MvnI 343 CGCG CG^CG NcoI 641 CCATGG C^CATGG NheI 1185 GCTAGC G^CTAGC NspI 100 ACATGT [GA]CATG^[CT] PaeR7I 251 CTCGAG C^TCGAG SacI 715 GAGCTC GAGCT^C SfcI 1204 CTACAG C^T[GA][CT]AG StyI 641 CCATGG C^C[AT][AT]GG Tsp45I 569 GTGAC ^GT[GC]AC TspRI 214 CACTG CA[GC]TG[ACGT][ACGT]^ XbaI 915 TCTAGA T^CTAGA XcmI 351 CCAGCAGCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 251 CTCGAG C^TCGAG XhoII 56 AGATCT [GA]^GATC[CT] XmnI 40 GAAATTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AcyI 381 GACGCC G[GA]^CG[CT]C 1000 GACGTC AflIII 100 ACATGT A^C[GA][CT]GT 1197 ACACGT AocI 460 CCTGAGG CC^T[ACGT]AGG 1013 CCTTAGG BbsI 1024 GAAGAC GAAGAC(2/6) 1003 GTCTTC BfaI 916 CTAG C^TAG 1186 CTAG BmyI 451 GGGCAC G[AGT]GC[ACT]^C 715 GAGCTC BpmI 718 CTCCAG CTGGAG(16/14) 362 CTGGAG BpuAI 1024 GAAGAC GAAGAC(2/6) 1003 GTCTTC BsaHI 381 GACGCC G[GA]^CG[CT]C 1000 GACGTC BsiYI 458 CCCCTGAGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 580 CCAGCATCAGG BslI 458 CCCCTGAGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 580 CCAGCATCAGG Bsp1286I 451 GGGCAC G[AGT]GC[ACT]^C 715 GAGCTC BspMI 694 ACCTGC ACCTGC(4/8) 907 GCAGGT BsrDI 143 GCAATG GCAATG(2/0) 1387 GCAATG BsrI 194 CCAGT ACTGG(1/-1) 525 CCAGT BssKI 361 CCTGG ^CC[ACGT]GG 501 CCTGG BstF5I 245 GGATG GGATG(2/0) 326 GGATG BstNI 361 CCTGG CC^[AT]GG 501 CCTGG Bsu36I 460 CCTGAGG CC^T[ACGT]AGG 1013 CCTTAGG EaeI 270 TGGCCG [CT]^GGCC[GA] 401 CGGCCA EarI 1190 CTCTTC CTCTTC(1/4) 712 GAAGAG Eco81I 460 CCTGAGG CC^T[ACGT]AGG 1013 CCTTAGG EcoRII 361 CCTGG ^CC[AT]GG 501 CCTGG FokI 245 GGATG GGATG(9/13) 326 GGATG HaeIII 271 GGCC GG^CC 402 GGCC Hin1I 381 GACGCC G[GA]^CG[CT]C 1000 GACGTC Ksp632I 1190 CTCTTC CTCTTC(1/4) 712 GAAGAG MaeI 916 CTAG C^TAG 1186 CTAG MaeIII 437 GTAAC ^GT[ACGT]AC 569 GTGAC MvaI 361 CCTGG CC^[AT]GG 501 CCTGG PleI 478 GACTC GAGTC(4/5) 952 GACTC SapI 712 GAAGAGC GCTCTTC(1/4) 1189 GCTCTTC ScrFI 361 CCTGG CC^[ACGT]GG 501 CCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 33 enzymes) # ######################################### AciI AcsI AfaI AluI ApoI BbvI BsmAI Cac8I DdeI DpnII Fnu4HI HinfI HphI ItaI MaeII MboI MboII MnlI MseI MspA1I MwoI NdeII NlaIII RsaI Sau3AI SfaNI SmlI TaqI TfiI Tru9I TseI Tsp509I TthHB8I