(Circular) (Six-base) MAPSORT of: pFN21AE2482.seq Check: 8461 from: 1 to: 6109 pFN21AE2482 6109 bp With 182 enzymes: SgfI * September 20, 2013 17:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3009 3919 278 Size: 53 83 186 936 1473 910 2468 Fragments arranged by size: 2468 1473 936 910 186 83 53 AccI GT'mk_AC Cuts at: 1937 3263 3452 1937 Size: 1326 189 4594 Fragments arranged by size: 4594 1326 189 Acc65I G'GTAC_C Cuts at: 3430 3430 Size: 6109 AclI AA'CG_TT Cuts at: 1563 4236 4609 1563 Size: 2673 373 3063 Fragments arranged by size: 3063 2673 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3108 3360 4172 5556 5824 1632 Size: 1476 252 812 1384 268 1917 Fragments arranged by size: 1917 1476 1384 812 268 252 AflII C'TTAA_G Cuts at: 820 1017 3268 820 Size: 197 2251 3661 Fragments arranged by size: 3661 2251 197 AflIII A'CryG_T Cuts at: 1203 2105 3202 5008 5985 1203 Size: 902 1097 1806 977 1327 Fragments arranged by size: 1806 1327 1097 977 902 AgeI A'CCGG_T Cuts at: 4940 4940 Size: 6109 AhdI GACnn_n'nnGTC Cuts at: 4838 4838 Size: 6109 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6109 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2166 2823 1184 Size: 982 657 4470 Fragments arranged by size: 4470 982 657 AlwNI CAG_nnn'CTG Cuts at: 3852 5424 3852 Size: 1572 4537 ApaLI G'TGCA_C Cuts at: 4166 5322 4166 Size: 1156 4953 ApoI r'AATT_y Cuts at: 1493 2270 3053 3183 3380 3418 3644 3796 5905 Size: 777 783 130 197 38 226 152 2109 Cuts at: 5905 1493 Size: 1697 Fragments arranged by size: 2109 1697 783 777 226 197 152 130 38 AseI AT'TA_AT Cuts at: 160 1032 4663 160 Size: 872 3631 1606 Fragments arranged by size: 3631 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6109 AvaI C'yCGr_G Cuts at: 1870 1943 2256 3434 1870 Size: 73 313 1178 4545 Fragments arranged by size: 4545 1178 313 73 BamHI G'GATC_C Cuts at: 3439 3806 3439 Size: 367 5742 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3430 4885 618 Size: 325 217 627 1643 1455 1842 Fragments arranged by size: 1842 1643 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2724 3428 1391 Size: 1333 704 4072 Fragments arranged by size: 4072 1333 704 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3001 3036 928 Size: 971 1102 35 4001 Fragments arranged by size: 4001 1102 971 35 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4300 4334 1903 Size: 34 2363 34 3678 Fragments arranged by size: 3678 2363 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4001 5211 4001 Size: 1210 4899 BclI T'GATC_A Cuts at: 1548 2890 1548 Size: 1342 4767 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4720 136 Size: 107 122 71 631 605 3048 1525 Fragments arranged by size: 3048 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2061 6104 2061 Size: 4043 2066 BlpI GC'TnA_GC Cuts at: 2376 3526 2376 Size: 1150 4959 Bme1580I G_kGCm'C Cuts at: 1790 2460 4170 5326 1790 Size: 670 1710 1156 2573 Fragments arranged by size: 2573 1710 1156 670 BmgBI CAC'GTC Cuts at: 1318 3205 1318 Size: 1887 4222 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3840 4798 442 Size: 416 53 83 399 24 2423 958 1753 Fragments arranged by size: 2423 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3194 1056 Size: 2138 3971 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2738 2738 Size: 6109 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2388 2708 4769 1239 Size: 1149 320 2061 2579 Fragments arranged by size: 2579 2061 1149 320 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2731 4234 5099 5397 2731 Size: 1503 865 298 3443 Fragments arranged by size: 3443 1503 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4772 882 Size: 617 3273 2219 Fragments arranged by size: 3273 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3900 493 Size: 711 2696 2702 Fragments arranged by size: 2702 2696 711 BsaBI GATnn'nnATC Cuts at: 6103 6103 Size: 6109 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2387 3006 3916 4298 Size: 53 83 186 936 854 619 910 382 Cuts at: 4298 4933 275 Size: 635 1451 Fragments arranged by size: 1451 936 910 854 635 619 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2328 4542 4940 5214 5361 5772 2328 Size: 2214 398 274 147 411 2665 Fragments arranged by size: 2665 2214 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2780 2810 1178 Size: 30 165 30 1377 30 4477 Fragments arranged by size: 4477 1377 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6109 BseYI C'CCAG_C Cuts at: 5312 5808 5312 Size: 496 5613 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5536 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3559 4320 5348 1540 Size: 275 186 1558 761 1028 2301 Fragments arranged by size: 2301 1558 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2724 4170 4255 5326 2724 Size: 1446 85 1071 3507 Fragments arranged by size: 3507 1446 1071 85 BsmI GAATG_Cn' Cuts at: 3620 3713 3620 Size: 93 6016 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2460 2724 3428 4170 4255 5326 1391 Size: 399 670 264 704 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 704 670 399 264 85 BspEI T'CCGG_A Cuts at: 5772 5772 Size: 6109 BspHI T'CATG_A Cuts at: 3998 5728 3998 Size: 1730 4379 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2708 2903 3464 844 Size: 1864 195 561 3489 Fragments arranged by size: 3489 1864 561 195 BsrBI CCG'CTC Cuts at: 1394 1823 3996 1394 Size: 429 2173 3507 Fragments arranged by size: 3507 2173 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2155 3399 4604 4778 65 Size: 1183 907 1244 1205 174 1396 Fragments arranged by size: 1396 1244 1205 1183 907 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4753 4940 1700 Size: 110 2943 187 2869 Fragments arranged by size: 2943 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6109 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6109 BssSI C'ACGA_G Cuts at: 4169 5181 4169 Size: 1012 5097 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6109 BstBI TT'CG_AA Cuts at: 4913 4913 Size: 6109 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6109 BstYI r'GATC_y Cuts at: 1972 2061 3439 3806 4191 4208 5660 5746 5758 Size: 89 1378 367 385 17 1452 86 12 Cuts at: 5758 6104 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1378 385 367 346 89 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2466 3019 2466 Size: 553 5556 BtgI C'CryG_G Cuts at: 513 1065 2646 513 Size: 552 1581 3976 Fragments arranged by size: 3976 1581 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2042 487 Size: 683 56 816 4554 Fragments arranged by size: 4554 816 683 56 BtsI GCAGTG_nn' Cuts at: 3625 4419 4439 3625 Size: 794 20 5295 Fragments arranged by size: 5295 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6109 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2047 2082 529 Size: 35 1483 35 4556 Fragments arranged by size: 4556 1483 35 35 DraI TTT'AAA Cuts at: 3313 3413 3313 Size: 100 6009 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2489 5116 809 Size: 507 1173 2627 1802 Fragments arranged by size: 2627 1802 1173 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2275 2406 3556 Size: 54 1425 50 275 18 445 131 1150 Cuts at: 3556 4445 6074 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1150 889 445 275 131 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3556 1537 Size: 275 1744 4090 Fragments arranged by size: 4090 1744 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2712 3201 4039 1353 Size: 1359 489 838 3423 Fragments arranged by size: 3423 1359 838 489 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2476 4696 5070 5216 387 Size: 1064 138 887 2220 374 146 1280 Fragments arranged by size: 2220 1280 1064 887 374 146 138 EcoICRI GAG'CTC Cuts at: 2722 2722 Size: 6109 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2388 2708 3108 3360 4172 4769 5556 Size: 393 756 320 400 252 812 597 787 Cuts at: 5556 5824 1239 Size: 268 1524 Fragments arranged by size: 1524 812 787 756 597 400 393 320 268 252 EcoRI G'AATT_C Cuts at: 3418 3796 3418 Size: 378 5731 EcoRV GAT'ATC Cuts at: 2545 2545 Size: 6109 FspI TGC'GCA Cuts at: 4615 4615 Size: 6109 HaeII r_GCGC'y Cuts at: 1678 5256 1678 Size: 3578 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2020 2052 2063 2095 4750 Size: 32 135 32 448 32 11 32 2655 Cuts at: 4750 4782 4824 4856 1373 Size: 32 42 32 2626 Fragments arranged by size: 2655 2626 448 135 42 32 32 32 32 32 32 11 HincII GTy'rAC Cuts at: 669 1657 1938 3453 3699 669 Size: 988 281 1515 246 3079 Fragments arranged by size: 3079 1515 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6109 HpaI GTT'AAC Cuts at: 3699 3699 Size: 6109 KpnI G_GTAC'C Cuts at: 3434 3434 Size: 6109 MfeI C'AATT_G Cuts at: 2801 3708 2801 Size: 907 5202 MluI A'CGCG_T Cuts at: 5985 5985 Size: 6109 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5223 5407 1305 Size: 3918 184 2007 Fragments arranged by size: 3918 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4287 Fragments arranged by size: 4287 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2094 4067 4426 4585 518 Size: 648 928 1973 359 159 2042 Fragments arranged by size: 2042 1973 928 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2211 2383 2785 3525 4202 5350 5595 1606 Size: 605 172 402 740 677 1148 245 2120 Fragments arranged by size: 2120 1148 740 677 605 402 245 172 NcoI C'CATG_G Cuts at: 513 1065 2646 513 Size: 552 1581 3976 Fragments arranged by size: 3976 1581 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6109 NheI G'CTAG_C Cuts at: 1052 3190 1052 Size: 2138 3971 NotI GC'GGCC_GC Cuts at: 3556 3556 Size: 6109 NspI r_CATG'y Cuts at: 1132 1229 2109 3467 5012 1132 Size: 97 880 1358 1545 2229 Fragments arranged by size: 2229 1545 1358 880 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6109 PciI A'CATG_T Cuts at: 2105 5008 2105 Size: 2903 3206 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6109 PmeI GTTT'AAAC Cuts at: 3413 3413 Size: 6109 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3055 3087 4178 4210 5691 5723 3055 Size: 32 1091 32 1481 32 3441 Fragments arranged by size: 3441 1481 1091 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6109 PsiI TTA'TAA Cuts at: 3679 3679 Size: 6109 PstI C_TGCA'G Cuts at: 830 3461 830 Size: 2631 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6109 SacI G_AGCT'C Cuts at: 2724 2724 Size: 6109 SalI G'TCGA_C Cuts at: 1936 3451 1936 Size: 1515 4594 SapI GCTCTTCn'nnn_ Cuts at: 2712 3201 2712 Size: 489 5620 SbfI CC_TGCA'GG Cuts at: 3461 3461 Size: 6109 ScaI AGT'ACT Cuts at: 4357 6069 4357 Size: 1712 4397 SfcI C'TryA_G Cuts at: 826 1045 3209 3457 4592 5273 5464 826 Size: 219 2164 248 1135 681 191 1471 Fragments arranged by size: 2164 1471 1135 681 248 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6109 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6109 SmaI CCC'GGG Cuts at: 1872 3436 1872 Size: 1564 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2256 2710 3268 4213 5114 5376 Size: 197 926 313 454 558 945 901 262 Cuts at: 5376 820 Size: 1553 Fragments arranged by size: 1553 945 926 901 558 454 313 262 197 SnaBI TAC'GTA Cuts at: 493 3900 493 Size: 3407 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6109 SphI G_CATG'C Cuts at: 1132 3467 1132 Size: 2335 3774 SspI AAT'ATT Cuts at: 5 52 4033 5 Size: 47 3981 2081 Fragments arranged by size: 3981 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6109 StyI C'CwwG_G Cuts at: 513 1065 2646 3548 513 Size: 552 1581 902 3074 Fragments arranged by size: 3074 1581 902 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4136 4153 4306 4491 4936 325 Size: 1255 2556 17 153 185 445 1498 Fragments arranged by size: 2556 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2317 2976 3118 Size: 274 80 33 51 1443 340 659 142 Cuts at: 3118 4355 6067 96 Size: 1237 1712 138 Fragments arranged by size: 1712 1443 1237 659 340 274 142 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2780 2812 6069 6101 2780 Size: 32 3257 32 2788 Fragments arranged by size: 3257 2788 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6109 XbaI T'CTAG_A Cuts at: 2920 3445 2920 Size: 525 5584 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2363 2363 Size: 6109 XhoI C'TCGA_G Cuts at: 1943 2256 1943 Size: 313 5796 XmaI C'CCGG_G Cuts at: 1870 3434 1870 Size: 1564 4545 XmnI GAAnn'nnTTC Cuts at: 2049 4238 2049 Size: 2189 3920 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3007 3917 276 Size: 53 83 186 936 1473 910 2468 Fragments arranged by size: 2468 1473 936 910 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI