(Circular) (Six-base) MAPSORT of: pFN21AB5968.seq Check: 3531 from: 1 to: 6373 pFN21AB5968 6373 bp With 182 enzymes: SgfI * January 30, 2014 17:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2114 2128 2114 Size: 14 6359 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3273 4183 278 Size: 53 83 186 936 1737 910 2468 Fragments arranged by size: 2468 1737 936 910 186 83 53 AccI GT'mk_AC Cuts at: 1937 3527 3716 1937 Size: 1590 189 4594 Fragments arranged by size: 4594 1590 189 Acc65I G'GTAC_C Cuts at: 3694 3694 Size: 6373 AclI AA'CG_TT Cuts at: 1563 4500 4873 1563 Size: 2937 373 3063 Fragments arranged by size: 3063 2937 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2175 3372 3624 4436 5820 6088 1632 Size: 543 1197 252 812 1384 268 1917 Fragments arranged by size: 1917 1384 1197 812 543 268 252 AflII C'TTAA_G Cuts at: 820 1017 3532 820 Size: 197 2515 3661 Fragments arranged by size: 3661 2515 197 AflIII A'CryG_T Cuts at: 1203 2369 3466 5272 6249 1203 Size: 1166 1097 1806 977 1327 Fragments arranged by size: 1806 1327 1166 1097 977 AgeI A'CCGG_T Cuts at: 5204 5204 Size: 6373 AhdI GACnn_n'nnGTC Cuts at: 5102 5102 Size: 6373 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6373 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2430 3087 1184 Size: 1246 657 4470 Fragments arranged by size: 4470 1246 657 AlwNI CAG_nnn'CTG Cuts at: 4116 5688 4116 Size: 1572 4801 ApaI G_GGCC'C Cuts at: 2020 2020 Size: 6373 ApaLI G'TGCA_C Cuts at: 4430 5586 4430 Size: 1156 5217 ApoI r'AATT_y Cuts at: 1493 2534 3317 3447 3644 3682 3908 4060 6169 Size: 1041 783 130 197 38 226 152 2109 Cuts at: 6169 1493 Size: 1697 Fragments arranged by size: 2109 1697 1041 783 226 197 152 130 38 AseI AT'TA_AT Cuts at: 160 1032 4927 160 Size: 872 3895 1606 Fragments arranged by size: 3895 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6373 AvaI C'yCGr_G Cuts at: 1870 1943 2520 3698 1870 Size: 73 577 1178 4545 Fragments arranged by size: 4545 1178 577 73 BamHI G'GATC_C Cuts at: 3703 4070 3703 Size: 367 6006 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3694 5149 618 Size: 325 217 627 1907 1455 1842 Fragments arranged by size: 1907 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2020 2988 3692 1391 Size: 629 968 704 4072 Fragments arranged by size: 4072 968 704 629 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2072 2181 3265 3300 928 Size: 971 173 109 1084 35 4001 Fragments arranged by size: 4001 1084 971 173 109 35 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4564 4598 1903 Size: 34 2627 34 3678 Fragments arranged by size: 3678 2627 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2192 2282 4265 5475 2192 Size: 90 1983 1210 3090 Fragments arranged by size: 3090 1983 1210 90 BclI T'GATC_A Cuts at: 1548 3154 1548 Size: 1606 4767 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4984 136 Size: 107 122 71 631 605 3312 1525 Fragments arranged by size: 3312 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2325 6368 2325 Size: 4043 2330 BlpI GC'TnA_GC Cuts at: 2640 3790 2640 Size: 1150 5223 Bme1580I G_kGCm'C Cuts at: 1790 2020 2724 4434 5590 1790 Size: 230 704 1710 1156 2573 Fragments arranged by size: 2573 1710 1156 704 230 BmgBI CAC'GTC Cuts at: 1318 3469 1318 Size: 2151 4222 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4104 5062 442 Size: 416 53 83 399 24 2687 958 1753 Fragments arranged by size: 2687 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3458 1056 Size: 2402 3971 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3002 3002 Size: 6373 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2251 2652 2972 5033 1239 Size: 1012 401 320 2061 2579 Fragments arranged by size: 2579 2061 1012 401 320 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2995 4498 5363 5661 2995 Size: 1503 865 298 3707 Fragments arranged by size: 3707 1503 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5036 882 Size: 617 3537 2219 Fragments arranged by size: 3537 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4164 493 Size: 711 2960 2702 Fragments arranged by size: 2960 2702 711 BsaBI GATnn'nnATC Cuts at: 6367 6367 Size: 6373 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2217 2651 3270 4180 Size: 53 83 186 936 684 434 619 910 Cuts at: 4180 4562 5197 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 910 684 635 619 434 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2592 4806 5204 5478 5625 6036 2592 Size: 2214 398 274 147 411 2929 Fragments arranged by size: 2929 2214 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3044 3074 1178 Size: 30 165 30 1641 30 4477 Fragments arranged by size: 4477 1641 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6373 BseYI C'CCAG_C Cuts at: 5576 6072 5576 Size: 496 5877 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5800 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3823 4584 5612 1540 Size: 275 186 1822 761 1028 2301 Fragments arranged by size: 2301 1822 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2988 4434 4519 5590 2988 Size: 1446 85 1071 3771 Fragments arranged by size: 3771 1446 1071 85 BsmI GAATG_Cn' Cuts at: 2302 3884 3977 2302 Size: 1582 93 4698 Fragments arranged by size: 4698 1582 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2020 2724 2988 3692 4434 4519 5590 Size: 399 230 704 264 704 742 85 1071 Cuts at: 5590 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 704 704 399 264 230 85 BspEI T'CCGG_A Cuts at: 6036 6036 Size: 6373 BspHI T'CATG_A Cuts at: 4262 5992 4262 Size: 1730 4643 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2114 2128 2972 3167 3728 844 Size: 1270 14 844 195 561 3489 Fragments arranged by size: 3489 1270 844 561 195 14 BsrBI CCG'CTC Cuts at: 1394 1823 4260 1394 Size: 429 2437 3507 Fragments arranged by size: 3507 2437 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2419 3663 4868 5042 65 Size: 1183 1171 1244 1205 174 1396 Fragments arranged by size: 1396 1244 1205 1183 1171 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5017 5204 1700 Size: 110 3207 187 2869 Fragments arranged by size: 3207 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6373 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6373 BssSI C'ACGA_G Cuts at: 4433 5445 4433 Size: 1012 5361 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6373 BstBI TT'CG_AA Cuts at: 2083 5177 2083 Size: 3094 3279 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6373 BstYI r'GATC_y Cuts at: 1972 2325 3703 4070 4455 4472 5924 6010 6022 Size: 353 1378 367 385 17 1452 86 12 Cuts at: 6022 6368 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1378 385 367 353 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2730 3283 2730 Size: 553 5820 BtgI C'CryG_G Cuts at: 513 1065 2057 2910 513 Size: 552 992 853 3976 Fragments arranged by size: 3976 992 853 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5634 Fragments arranged by size: 5634 683 56 BtsI GCAGTG_nn' Cuts at: 3889 4683 4703 3889 Size: 794 20 5559 Fragments arranged by size: 5559 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6373 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2311 2346 529 Size: 35 1747 35 4556 Fragments arranged by size: 4556 1747 35 35 DraI TTT'AAA Cuts at: 3577 3677 3577 Size: 100 6273 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2753 5380 809 Size: 507 1437 2627 1802 Fragments arranged by size: 2627 1802 1437 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2539 2670 3820 Size: 54 1425 50 275 18 709 131 1150 Cuts at: 3820 4709 6338 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1150 889 709 275 131 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3820 1537 Size: 275 2008 4090 Fragments arranged by size: 4090 2008 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2976 3465 4303 1353 Size: 1623 489 838 3423 Fragments arranged by size: 3423 1623 838 489 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2740 4960 5334 5480 387 Size: 1064 138 1151 2220 374 146 1280 Fragments arranged by size: 2220 1280 1151 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2986 2986 Size: 6373 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2175 2251 2652 2972 3372 3624 4436 Size: 393 543 76 401 320 400 252 812 Cuts at: 4436 5033 5820 6088 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 812 787 597 543 401 400 393 320 268 252 76 EcoO109I rG'GnC_Cy Cuts at: 2016 2017 2016 Size: 1 6372 EcoRI G'AATT_C Cuts at: 3682 4060 3682 Size: 378 5995 EcoRV GAT'ATC Cuts at: 2809 2809 Size: 6373 FspI TGC'GCA Cuts at: 4879 4879 Size: 6373 HaeII r_GCGC'y Cuts at: 1678 5520 1678 Size: 3842 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2327 2359 5014 5046 5088 Size: 32 135 32 755 32 2655 32 42 Cuts at: 5088 5120 1373 Size: 32 2626 Fragments arranged by size: 2655 2626 755 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3717 3963 669 Size: 988 281 1779 246 3079 Fragments arranged by size: 3079 1779 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6373 HpaI GTT'AAC Cuts at: 3963 3963 Size: 6373 KpnI G_GTAC'C Cuts at: 3698 3698 Size: 6373 MfeI C'AATT_G Cuts at: 3065 3972 3065 Size: 907 5466 MluI A'CGCG_T Cuts at: 6249 6249 Size: 6373 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5487 5671 1305 Size: 4182 184 2007 Fragments arranged by size: 4182 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4551 Fragments arranged by size: 4551 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2358 4331 4690 4849 518 Size: 648 1192 1973 359 159 2042 Fragments arranged by size: 2042 1973 1192 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2475 2647 3049 3789 4466 5614 5859 1606 Size: 869 172 402 740 677 1148 245 2120 Fragments arranged by size: 2120 1148 869 740 677 402 245 172 NcoI C'CATG_G Cuts at: 513 1065 2910 513 Size: 552 1845 3976 Fragments arranged by size: 3976 1845 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6373 NheI G'CTAG_C Cuts at: 1052 3454 1052 Size: 2402 3971 NotI GC'GGCC_GC Cuts at: 3820 3820 Size: 6373 NspI r_CATG'y Cuts at: 1132 1229 2373 3731 5276 1132 Size: 97 1144 1358 1545 2229 Fragments arranged by size: 2229 1545 1358 1144 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6373 PciI A'CATG_T Cuts at: 2369 5272 2369 Size: 2903 3470 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6373 PfoI T'CCnGG_A Cuts at: 2228 2228 Size: 6373 PmeI GTTT'AAAC Cuts at: 3677 3677 Size: 6373 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3319 3351 4442 4474 5955 5987 3319 Size: 32 1091 32 1481 32 3705 Fragments arranged by size: 3705 1481 1091 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6373 PsiI TTA'TAA Cuts at: 3943 3943 Size: 6373 PspOMI G'GGCC_C Cuts at: 2016 2016 Size: 6373 PstI C_TGCA'G Cuts at: 830 2125 3725 830 Size: 1295 1600 3478 Fragments arranged by size: 3478 1600 1295 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6373 SacI G_AGCT'C Cuts at: 2988 2988 Size: 6373 SalI G'TCGA_C Cuts at: 1936 3715 1936 Size: 1779 4594 SapI GCTCTTCn'nnn_ Cuts at: 2976 3465 2976 Size: 489 5884 SbfI CC_TGCA'GG Cuts at: 2125 3725 2125 Size: 1600 4773 ScaI AGT'ACT Cuts at: 4621 6333 4621 Size: 1712 4661 SfcI C'TryA_G Cuts at: 826 1045 2121 3473 3721 4856 5537 5728 826 Size: 219 1076 1352 248 1135 681 191 1471 Fragments arranged by size: 1471 1352 1135 1076 681 248 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6373 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6373 SmaI CCC'GGG Cuts at: 1872 3700 1872 Size: 1828 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2520 2974 3532 4477 5378 5640 Size: 197 926 577 454 558 945 901 262 Cuts at: 5640 820 Size: 1553 Fragments arranged by size: 1553 945 926 901 577 558 454 262 197 SnaBI TAC'GTA Cuts at: 493 4164 493 Size: 3671 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6373 SphI G_CATG'C Cuts at: 1132 3731 1132 Size: 2599 3774 SspI AAT'ATT Cuts at: 5 52 4297 5 Size: 47 4245 2081 Fragments arranged by size: 4245 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6373 StyI C'CwwG_G Cuts at: 513 1065 2910 3812 513 Size: 552 1845 902 3074 Fragments arranged by size: 3074 1845 902 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2128 4400 4417 4570 4755 5200 325 Size: 1255 548 2272 17 153 185 445 1498 Fragments arranged by size: 2272 1498 1255 548 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2278 2581 3240 Size: 274 80 33 51 1443 301 303 659 Cuts at: 3240 3382 4619 6331 96 Size: 142 1237 1712 138 Fragments arranged by size: 1712 1443 1237 659 303 301 274 142 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3044 3076 6333 6365 3044 Size: 32 3257 32 3052 Fragments arranged by size: 3257 3052 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6373 XbaI T'CTAG_A Cuts at: 3184 3709 3184 Size: 525 5848 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2627 2627 Size: 6373 XhoI C'TCGA_G Cuts at: 1943 2520 1943 Size: 577 5796 XmaI C'CCGG_G Cuts at: 1870 3698 1870 Size: 1828 4545 XmnI GAAnn'nnTTC Cuts at: 2313 4502 2313 Size: 2189 4184 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3271 4181 276 Size: 53 83 186 936 1737 910 2468 Fragments arranged by size: 2468 1737 936 910 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI