Restriction map for pFN21AE1680
 (Circular) (Six-base) MAPSORT of: pFN21AE1680.seq  Check: 8446  from: 1  to: 5287



pFN21AE1680  5287 bp

 With 182 enzymes: SgfI * 

                        August 16, 2010 14:48  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3097     278 
   Size:         53      83     186     936    1561    2468

  Fragments arranged by size: 

                2468    1561     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2630    1937 
   Size:        693    4594
 
Acc65I G'GTAC_C

Cuts at:   2229    2608    2229 
   Size:        379    4908
 
AclI AA'CG_TT

Cuts at:   1563    3414    3787    1563 
   Size:       1851     373    3063

  Fragments arranged by size: 

                3063    1851     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    3350    4734    5002    1632 
   Size:       1718    1384     268    1917

  Fragments arranged by size: 

                1917    1718    1384     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5090
 
AflIII A'CryG_T

Cuts at:   1203    4186    5163    1203 
   Size:       2983     977    1327

  Fragments arranged by size: 

                2983    1327     977
 
AgeI A'CCGG_T

Cuts at:   4118    4118 
   Size:       5287
 
AhdI GACnn_n'nnGTC

Cuts at:   4016    4016 
   Size:       5287
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5287
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5287
 
AlwNI CAG_nnn'CTG

Cuts at:   3030    4602    3030 
   Size:       1572    3715
 
ApaLI G'TGCA_C

Cuts at:   3344    4500    3344 
   Size:       1156    4131
 
ApoI r'AATT_y

Cuts at:   1493    2351    2596    2822    2974    5083    1493 
   Size:        858     245     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     858     245     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    3841     160 
   Size:        872    2809    1606

  Fragments arranged by size: 

                2809    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5287
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2612    1870 
   Size:         73     669    4545

  Fragments arranged by size: 

                4545     669      73
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:   2213    2246    2213 
   Size:         33    5254
 
BamHI G'GATC_C

Cuts at:   2028    2617    2984    2028 
   Size:        589     367    4331

  Fragments arranged by size: 

                4331     589     367
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2229    2608    4063     618 
   Size:        325     217     627     442     379    1455    1842

  Fragments arranged by size: 

                1842    1455     627     442     379     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2606    1391 
   Size:       1215    4072
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2212     928 
   Size:        971     313    4003

  Fragments arranged by size: 

                4003     971     313
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    1987    2021    3478    3512    1903 
   Size:         34      50      34    1457      34    3678

  Fragments arranged by size: 

                3678    1457      50      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   2010    3179    4389    2010 
   Size:       1169    1210    2908

  Fragments arranged by size: 

                2908    1210    1169
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5287
 
BfrBI ATG'CAT

Cuts at:   2459    2459 
   Size:       5287
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    3898     136 
   Size:        107     122      71     631     605    2226    1525

  Fragments arranged by size: 

                2226    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5282    5282 
   Size:       5287
 
BlpI GC'TnA_GC

Cuts at:   2704    2704 
   Size:       5287
 
Bme1580I G_kGCm'C

Cuts at:   1790    3348    4504    1790 
   Size:       1558    1156    2573

  Fragments arranged by size: 

                2573    1558    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5287
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3018    3976     442 
   Size:        416      53      83     399      24    1601     958    1753

  Fragments arranged by size: 

                1753    1601     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5287
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2097    3947    1239 
   Size:        858    1850    2579

  Fragments arranged by size: 

                2579    1850     858
 
Bpu10I CC'TnA_GC

Cuts at:   2151    2151 
   Size:       5287
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3412    4277    4575    3412 
   Size:        865     298    4124

  Fragments arranged by size: 

                4124     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    3950     882 
   Size:        617    2451    2219

  Fragments arranged by size: 

                2451    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3078     493 
   Size:        711    1874    2702

  Fragments arranged by size: 

                2702    1874     711
 
BsaBI GATnn'nnATC

Cuts at:   5281    5281 
   Size:       5287
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3094    3476    4111     275 
   Size:         53      83     186     936    1561     382     635    1451

  Fragments arranged by size: 

                1561    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   3720    4118    4392    4539    4950    3720 
   Size:        398     274     147     411    4057

  Fragments arranged by size: 

                4057     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5062

  Fragments arranged by size: 

                5062     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    1186 
   Size:       5287
 
BseYI C'CCAG_C

Cuts at:   4490    4986    4490 
   Size:        496    4791
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    4714
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2737    3498    4526    1540 
   Size:        275     186     736     761    1028    2301

  Fragments arranged by size: 

                2301    1028     761     736     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   3348    3433    4504    3348 
   Size:         85    1071    4131

  Fragments arranged by size: 

                4131    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2405    2798    2891    2405 
   Size:        393      93    4801

  Fragments arranged by size: 

                4801     393      93
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2606    3348    3433    4504    1391 
   Size:        399     816     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     816     742     399      85
 
BspEI T'CCGG_A

Cuts at:   4950    4950 
   Size:       5287
 
BspHI T'CATG_A

Cuts at:   3176    4906    3176 
   Size:       1730    3557
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2642     844 
   Size:       1798    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3174    1394 
   Size:        429    1351    3507

  Fragments arranged by size: 

                3507    1351     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2107    2370    2410    3782    3956      65 
   Size:       1183     859     263      40    1372     174    1396

  Fragments arranged by size: 

                1396    1372    1183     859     263     174      40
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    3931    4118    1700 
   Size:        110    2121     187    2869

  Fragments arranged by size: 

                2869    2121     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5287
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5287
 
BssSI C'ACGA_G

Cuts at:   3347    4359    3347 
   Size:       1012    4275
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5287
 
BstBI TT'CG_AA

Cuts at:   4091    4091 
   Size:       5287
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5287
 
BstYI r'GATC_y

Cuts at:   1972    2028    2617    2984    3369    3386    4838    4924    4936 
   Size:         56     589     367     385      17    1452      86      12

Cuts at:   4936    5282    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1452     589     385     367     346      86      56
                  17      12
 
Bsu36I CC'TnA_GG

Cuts at:   2234    2234 
   Size:       5287
 
BtgI C'CryG_G

Cuts at:    513    1065     513 
   Size:        552    4735
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4548

  Fragments arranged by size: 

                4548     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2803    3597    3617    2803 
   Size:        794      20    4473

  Fragments arranged by size: 

                4473     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    2241    1554 
   Size:        687    4600
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5252
 
DraI TTT'AAA

Cuts at:   2591    2591 
   Size:       5287
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    2214    4294     809 
   Size:        507     898    2080    1802

  Fragments arranged by size: 

                2080    1802     898     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2734    3623    5252 
   Size:         54    1425      50     275      18     904     889    1629

Cuts at:   5252       8 
   Size:         43

  Fragments arranged by size: 

                1629    1425     904     889     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2734    1537 
   Size:        275     922    4090

  Fragments arranged by size: 

                4090     922     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2216    3217    1353 
   Size:        863    1001    3423

  Fragments arranged by size: 

                3423    1001     863
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    3874    4248    4394     387 
   Size:       1064     138    2285     374     146    1280

  Fragments arranged by size: 

                2285    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2097    3350    3947    4734    5002    1239 
   Size:        393     465    1253     597     787     268    1524

  Fragments arranged by size: 

                1524    1253     787     597     465     393     268
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2526    2526 
   Size:       5287
 
EcoO109I rG'GnC_Cy

Cuts at:   2265    2265 
   Size:       5287
 
EcoRI G'AATT_C

Cuts at:   2351    2596    2974    2351 
   Size:        245     378    4664

  Fragments arranged by size: 

                4664     378     245
 
FspI TGC'GCA

Cuts at:   3793    3793 
   Size:       5287
 
HaeII r_GCGC'y

Cuts at:   1678    4434    1678 
   Size:       2756    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    3928    3960    4002    4034    1373 
   Size:         32     135      32    2356      32      42      32    2626

  Fragments arranged by size: 

                2626    2356     135      42      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2631    2877     669 
   Size:        988     281     693     246    3079

  Fragments arranged by size: 

                3079     988     693     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5287
 
HpaI GTT'AAC

Cuts at:   2877    2877 
   Size:       5287
 
KpnI G_GTAC'C

Cuts at:   2233    2612    2233 
   Size:        379    4908
 
MfeI C'AATT_G

Cuts at:   2886    2886 
   Size:       5287
 
MluI A'CGCG_T

Cuts at:   5163    5163 
   Size:       5287
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2299    4401    4585    1305 
   Size:        994    2102     184    2007

  Fragments arranged by size: 

                2102    2007     994     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3465

  Fragments arranged by size: 

                3465    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    3245    3604    3763     518 
   Size:        648    2079     359     159    2042

  Fragments arranged by size: 

                2079    2042     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2703    3380    4528    4773    1606 
   Size:       1097     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148    1097     677     245
 
NcoI C'CATG_G

Cuts at:    513    1065     513 
   Size:        552    4735
 
NdeI CA'TA_TG

Cuts at:    387    2455     387 
   Size:       2068    3219
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5287
 
NotI GC'GGCC_GC

Cuts at:   2734    2734 
   Size:       5287
 
NsiI A_TGCA'T

Cuts at:   2461    2461 
   Size:       5287
 
NspI r_CATG'y

Cuts at:   1132    1229    2645    4190    1132 
   Size:         97    1416    1545    2229

  Fragments arranged by size: 

                2229    1545    1416      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5287
 
PciI A'CATG_T

Cuts at:   4186    4186 
   Size:       5287
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5287
 
PmeI GTTT'AAAC

Cuts at:   2591    2591 
   Size:       5287
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3356    3388    4869    4901    3356 
   Size:         32    1481      32    3742

  Fragments arranged by size: 

                3742    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2265    2265 
   Size:       5287
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5287
 
PsiI TTA'TAA

Cuts at:   2857    2857 
   Size:       5287
 
PstI C_TGCA'G

Cuts at:    830    2639     830 
   Size:       1809    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5287
 
SalI G'TCGA_C

Cuts at:   1936    2629    1936 
   Size:        693    4594
 
SanDI GG'GwC_CC

Cuts at:   2265    2265 
   Size:       5287
 
SbfI CC_TGCA'GG

Cuts at:   2639    2639 
   Size:       5287
 
ScaI AGT'ACT

Cuts at:   3535    5247    3535 
   Size:       1712    3575
 
SfcI C'TryA_G

Cuts at:    826    1045    2047    2635    3770    4451    4642     826 
   Size:        219    1002     588    1135     681     191    1471

  Fragments arranged by size: 

                1471    1135    1002     681     588     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5287
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5287
 
SmaI CCC'GGG

Cuts at:   1872    2614    1872 
   Size:        742    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3391    4292    4554     820 
   Size:        197     926    1448     901     262    1553

  Fragments arranged by size: 

                1553    1448     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3078     493 
   Size:       2585    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5287
 
SphI G_CATG'C

Cuts at:   1132    2645    1132 
   Size:       1513    3774
 
SspI AAT'ATT

Cuts at:      5      52    3211       5 
   Size:         47    3159    2081

  Fragments arranged by size: 

                3159    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5287
 
StyI C'CwwG_G

Cuts at:    513    1065    2726     513 
   Size:        552    1661    3074

  Fragments arranged by size: 

                3074    1661     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3314    3331    3484    3669    4114     325 
   Size:       1255    1734      17     153     185     445    1498

  Fragments arranged by size: 

                1734    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3533    5245      96 
   Size:        274      80      33      51    1443    1556    1712     138

  Fragments arranged by size: 

                1712    1556    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5247    5279    5247 
   Size:         32    5255
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5287
 
XbaI T'CTAG_A

Cuts at:   2623    2623 
   Size:       5287
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5287
 
XmaI C'CCGG_G

Cuts at:   1870    2612    1870 
   Size:        742    4545
 
XmnI GAAnn'nnTTC

Cuts at:   2430    3416    2430 
   Size:        986    4301
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3095     276 
   Size:         53      83     186     936    1561    2468

  Fragments arranged by size: 

                2468    1561     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI     BaeI    BamHI     BanI    BanII     BbsI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI
     EarI     EciI  Eco57MI    EcoNI EcoO109I    EcoRI     FspI    HaeII
    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI
     MscI     MslI   MspA1I     NcoI     NdeI     NheI     NotI     NsiI
     NspI     PasI     PciI    PflMI     PmeI     PpiI    PpuMI    PshAI
     PsiI     PstI     PvuI     SalI    SanDI     SbfI     ScaI     SfcI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SspI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XhoI
     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BbeI    BbvCI
     BplI    BsiWI    BsmBI    BstXI  BstZ17I   DraIII  EcoICRI    EcoRV
     FalI     FseI    FspAI     KasI     NaeI     NarI   NgoMIV     NruI
     PacI     PfoI     PmlI   PspOMI    PspXI     PsrI    PvuII    RsrII
     SacI    SacII     SapI    SexAI     SfiI     SfoI     SrfI     SwaI
     XcmI
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