Restriction map for pFN21AE9512
 (Linear) (Six-base) MAPSORT of: KIEE9512.seq  Check: 1014  from: 1  to: 1596



KIEE9512.orf

 With 182 enzymes: SgfI * 

                        December 27, 2011 16:41  ..

AccI GT'mk_AC

Cuts at:      0     971    1596 
   Size:        971     625
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1037    1488    1596 
   Size:       1037     451     108

  Fragments arranged by size: 

                1037     451     108
 
AleI CACnn'nnGTG

Cuts at:      0     133    1596 
   Size:        133    1463
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     516    1596 
   Size:        516    1080
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     790     822    1596 
   Size:        790      32     774

  Fragments arranged by size: 

                 790     774      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     755    1099    1329    1596 
   Size:        755     344     230     267

  Fragments arranged by size: 

                 755     344     267     230
 
ApaI G_GGCC'C

Cuts at:      0     489    1596 
   Size:        489    1107
 
ApoI r'AATT_y

Cuts at:      0    1006    1596 
   Size:       1006     590
 
AvaI C'yCGr_G

Cuts at:      0     292    1596 
   Size:        292    1304
 
BanI G'GyrC_C

Cuts at:      0      40    1596 
   Size:         40    1556
 
BanII G_rGCy'C

Cuts at:      0     291     489     572     744    1596 
   Size:        291     198      83     172     852

  Fragments arranged by size: 

                 852     291     198     172      83
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1516    1596 
   Size:       1516      80
 
BclI T'GATC_A

Cuts at:      0     125     368    1596 
   Size:        125     243    1228

  Fragments arranged by size: 

                1228     243     125
 
BglI GCCn_nnn'nGGC

Cuts at:      0     880    1596 
   Size:        880     716
 
BglII A'GATC_T

Cuts at:      0     225    1596 
   Size:        225    1371
 
BlpI GC'TnA_GC

Cuts at:      0     208     213     890    1596 
   Size:        208       5     677     706

  Fragments arranged by size: 

                 706     677     208       5
 
Bme1580I G_kGCm'C

Cuts at:      0     489    1596 
   Size:        489    1107
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      40     851    1216    1411    1596 
   Size:         40     811     365     195     185

  Fragments arranged by size: 

                 811     365     195     185      40
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     673    1076    1596 
   Size:        673     403     520

  Fragments arranged by size: 

                 673     520     403
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     166    1596 
   Size:        166    1430
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1168    1596 
   Size:       1168     428
 
BsaAI yAC'GTr

Cuts at:      0     333    1596 
   Size:        333    1263
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     790     820    1596 
   Size:        790      30     776

  Fragments arranged by size: 

                 790     776      30
 
BseYI C'CCAG_C

Cuts at:      0     284     489     785    1264    1596 
   Size:        284     205     296     479     332

  Fragments arranged by size: 

                 479     332     296     284     205
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     242    1596 
   Size:        242    1354
 
BsiHKAI G_wGCw'C

Cuts at:      0     832    1596 
   Size:        832     764
 
Bsp1286I G_dGCh'C

Cuts at:      0     291     489     572     744     832    1596 
   Size:        291     198      83     172      88     764

  Fragments arranged by size: 

                 764     291     198     172      88      83
 
BspHI T'CATG_A

Cuts at:      0     152     353     446     623    1596 
   Size:        152     201      93     177     973

  Fragments arranged by size: 

                 973     201     177     152      93
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     212    1596 
   Size:        212    1384
 
BsrDI GCAATG_nn'

Cuts at:      0      95     147     593    1596 
   Size:         95      52     446    1003

  Fragments arranged by size: 

                1003     446      95      52
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     678     862    1344    1596 
   Size:        678     184     482     252

  Fragments arranged by size: 

                 678     482     252     184
 
BstYI r'GATC_y

Cuts at:      0     225     551     751    1596 
   Size:        225     326     200     845

  Fragments arranged by size: 

                 845     326     225     200
 
BtsI GCAGTG_nn'

Cuts at:      0     587    1596 
   Size:        587    1009
 
DraIII CAC_nnn'GTG

Cuts at:      0    1197    1333    1427    1596 
   Size:       1197     136      94     169

  Fragments arranged by size: 

                1197     169     136      94
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1115    1411    1596 
   Size:       1115     296     185

  Fragments arranged by size: 

                1115     296     185
 
EaeI y'GGCC_r

Cuts at:      0     335    1226    1596 
   Size:        335     891     370

  Fragments arranged by size: 

                 891     370     335
 
EarI CTCTTCn'nnn_

Cuts at:      0     715    1013    1553    1596 
   Size:        715     298     540      43

  Fragments arranged by size: 

                 715     540     298      43
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     673    1037    1076    1488    1596 
   Size:        673     364      39     412     108

  Fragments arranged by size: 

                 673     412     364     108      39
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     822    1596 
   Size:        822     774
 
EcoO109I rG'GnC_Cy

Cuts at:      0     486     675    1596 
   Size:        486     189     921

  Fragments arranged by size: 

                 921     486     189
 
EcoRI G'AATT_C

Cuts at:      0    1006    1596 
   Size:       1006     590
 
HaeII r_GCGC'y

Cuts at:      0     875    1596 
   Size:        875     721
 
HincII GTy'rAC

Cuts at:      0     171     192    1470    1596 
   Size:        171      21    1278     126

  Fragments arranged by size: 

                1278     171     126      21
 
HpaI GTT'AAC

Cuts at:      0     192    1596 
   Size:        192    1404
 
MfeI C'AATT_G

Cuts at:      0     876    1596 
   Size:        876     720
 
MscI TGG'CCA

Cuts at:      0     337    1228    1596 
   Size:        337     891     368

  Fragments arranged by size: 

                 891     368     337
 
MslI CAynn'nnrTG

Cuts at:      0     133     274    1596 
   Size:        133     141    1322

  Fragments arranged by size: 

                1322     141     133
 
MspA1I CmG'CkG

Cuts at:      0     103     789    1099    1596 
   Size:        103     686     310     497

  Fragments arranged by size: 

                 686     497     310     103
 
PasI CC'CwG_GG

Cuts at:      0     997    1596 
   Size:        997     599
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      63    1596 
   Size:         63    1533
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     790     822    1596 
   Size:        790      32     774

  Fragments arranged by size: 

                 790     774      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     675    1596 
   Size:        675     921
 
PspOMI G'GGCC_C

Cuts at:      0     485    1596 
   Size:        485    1111
 
PvuII CAG'CTG

Cuts at:      0     103    1099    1596 
   Size:        103     996     497

  Fragments arranged by size: 

                 996     497     103
 
ScaI AGT'ACT

Cuts at:      0     511    1596 
   Size:        511    1085
 
SfcI C'TryA_G

Cuts at:      0     906    1596 
   Size:        906     690
 
SmlI C'TyrA_G

Cuts at:      0     181    1596 
   Size:        181    1415
 
StuI AGG'CCT

Cuts at:      0     805    1250    1596 
   Size:        805     445     346

  Fragments arranged by size: 

                 805     445     346
 
StyI C'CwwG_G

Cuts at:      0     671    1229    1596 
   Size:        671     558     367

  Fragments arranged by size: 

                 671     558     367
 
TatI w'GTAC_w

Cuts at:      0     509    1568    1596 
   Size:        509    1059      28

  Fragments arranged by size: 

                1059     509      28
 
Tth111I GACn'n_nGTC

Cuts at:      0     955    1596 
   Size:        955     641
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     875    1424    1596 
   Size:        875     549     172

  Fragments arranged by size: 

                 875     549     172
 

 Enzymes that do cut:

     AccI     AcuI     AleI     AlfI     AloI    AlwNI     ApaI     ApoI
     AvaI     BanI    BanII     BbsI     BclI     BglI    BglII     BlpI
 Bme1580I     BmrI     BpmI    BpuEI     BsaI    BsaAI    BsaXI    BseYI
     BsgI  BsiHKAI Bsp1286I    BspHI    BspMI    BsrDI    BstXI    BstYI
     BtsI   DraIII     DrdI     EaeI     EarI  Eco57MI    EcoNI EcoO109I
    EcoRI    HaeII   HincII     HpaI     MfeI     MscI     MslI   MspA1I
     PasI    PflMI     PpiI    PpuMI   PspOMI    PvuII     ScaI     SfcI
     SmlI     StuI     StyI     TatI  Tth111I     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
     BbeI    BbvCI     BcgI    BciVI    BfrBI    BmgBI     BmtI     BplI
   Bpu10I    BsaBI    BsaHI    BsaWI    BseRI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI
     DraI     EagI     EciI  EcoICRI    EcoRV     FalI     FseI     FspI
    FspAI    Hin4I  HindIII     KasI     KpnI     MluI     MmeI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PciI     PfoI     PmeI     PmlI    PshAI     PsiI
    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI    TaqII
     TstI     XbaI     XhoI     XmaI     XmnI     ZraI
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