################################## # NO CUT ENZYMES ( 211 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaII AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmFI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrFI BsrGI BsrI BssHII BssKI BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstNI BstPI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DdeI DpnII DraI DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRII EcoRV EcoT14I EcoT22I FbaI FokI FseI FspI HapII HgaI Hin1I HincII HindII HindIII HinfI HpaI HpaII HphI KasI KpnI Ksp632I MaeII MaeIII MamI MboI MboII MfeI MflI MluI MluNI MroI MscI MslI MspI MunI MvaI NaeI NarI NciI NcoI NdeII NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsaI RsrII SacI SalI SapI Sau3AI SbfI ScaI ScrFI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 24 enzymes) # ################################### AluI 174 AGCT AG^CT ApaI 94 GGGCCC GGGCC^C Asp700I 198 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 90 CTCGGG C^[CT]CG[GA]G BanII 94 GGGCCC G[GA]GC[CT]^C BfaI 187 CTAG C^TAG BmyI 94 GGGCCC G[AGT]GC[ACT]^C BsaJI 122 CCGCGG C^C[ACGT][ACGT]GG BsoBI 90 CTCGGG C^[CT]CG[GA]G Bsp1286I 94 GGGCCC G[AGT]GC[ACT]^C BsrBI 71 CCGCTC CCGCTC(-3/-3) DsaI 122 CCGCGG C^C[GA][CT]GG HaeII 17 GGCGCT [GA]GCGC^[CT] KspI 122 CCGCGG CCGC^GG MaeI 187 CTAG C^TAG MnlI 4 CCTC CCTC(7/6) MseI 166 TTAA T^TAA NdeI 179 CATATG CA^TATG PstI 64 CTGCAG CTGCA^G PvuII 173 CAGCTG CAG^CTG SacII 122 CCGCGG CCGC^GG SfcI 64 CTGCAG C^T[GA][CT]AG Tru9I 166 TTAA T^TAA XmnI 198 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### AccII 101 CGCG CG^CG 123 CGCG BbvI 66 GCAGC GCAGC(8/12) 175 GCTGC BsiYI 42 CCGCCGCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 86 CCCACTCGGGG BslI 42 CCGCCGCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 86 CCCACTCGGGG BstUI 101 CGCG CG^CG 123 CGCG CfoI 18 GCGC GCG^C 102 GCGC DraII 93 GGGGCCC [GA]G^G[ACGT]CC[CT] 94 GGGCCCC EcoO109I 93 GGGGCCC [GA]G^G[ACGT]CC[CT] 94 GGGCCCC HhaI 18 GCGC GCG^C 102 GCGC HinP1I 18 GCGC G^CGC 102 GCGC MvnI 101 CGCG CG^CG 123 CGCG TseI 66 GCAGC G^C[AT]GC 175 GCTGC ######################################### # 3 OR MORE CUT ENZYMES ( 11 enzymes) # ######################################### AciI Cac8I Cfr13I FauI Fnu4HI HaeIII ItaI MspA1I MwoI NlaIV Sau96I