Restriction map for
pFN21AE2480
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# NO CUT ENZYMES ( 88 enzymes) #
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AatII Acc65I AccII AccIII AclI
AflII Alw44I AocI ApaI ApaLI
AscI Asp718I AviII AvrII BamHI
BbeI BbvCI BcgI BcnI BfrI
BglI BlnI BsaBI BseAI BsiEI
BsiWI BspDI BspEI BspHI BspLU11I
BsrBI BssHII BstBI BstUI Bsu36I
ClaI CpoI DraII DraIII DrdI
EagI EclXI Eco52I Eco81I EcoNI
EcoO109I FauI FseI FspI KasI
KpnI KspI MamI MluI MroI
MvnI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NspV
PacI PmeI PpuMI PshAI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI SbfI SfiI SfoI SfuI
SgrAI SmaI SnaBI SphI Sse8387I
SwaI XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AcyI 4286 GGCGTC G[GA]^CG[CT]C
AhdI 1539 GACACCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 421 AGCGCT AGC^GCT
Asp700I 3406 GAATCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1539 GACACCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1895 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 3341 CTCGAG C^[CT]CG[GA]G
BanI 884 GGCACC G^G[CT][GA]CC
BanII 3338 GAGCTC G[GA]GC[CT]^C
BclI 2 TGATCA T^GATCA
BsaHI 4286 GGCGTC G[GA]^CG[CT]C
BsmBI 4288 CGTCTC CGTCTC(1/5)
BsmI 1016 GCATTC GAATGC(1/-1)
BsoBI 3341 CTCGAG C^[CT]CG[GA]G
Bsp1407I 1319 TGTACA T^GTACA
BsrGI 1319 TGTACA T^GTACA
BssSI 3655 CTCGTG CACGAG(-5/-1)
Bst1107I 3689 GTATAC GTA^TAC
BstEII 3040 GGTGACC G^GT[ACGT]ACC
BstPI 3040 GGTGACC G^GT[ACGT]ACC
BstXI 311 CCAGTGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 3689 GTATAC GTA^TAC
CfoI 422 GCGC GCG^C
Eam1105I 1539 GACACCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 421 AGCGCT AGC^GCT
EcoO65I 3040 GGTGACC G^GT[ACGT]ACC
EcoRV 2892 GATATC GAT^ATC
FbaI 2 TGATCA T^GATCA
HaeII 421 AGCGCT [GA]GCGC^[CT]
HgaI 4287 GCGTC GACGC(5/10)
HhaI 422 GCGC GCG^C
Hin1I 4286 GGCGTC G[GA]^CG[CT]C
HinP1I 422 GCGC G^CGC
HpaI 2742 GTTAAC GTT^AAC
NspI 1177 GCATGT [GA]CATG^[CT]
PaeR7I 3341 CTCGAG C^TCGAG
PflFI 1895 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 841 CCAAAGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 4162 CAGCTG CAG^CTG
SacI 3338 GAGCTC GAGCT^C
ScaI 1591 AGTACT AGT^ACT
SexAI 1896 ACCTGGT A^CC[AT]GGT
SspBI 1319 TGTACA T^GTACA
Tth111I 1895 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 841 CCAAAGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1572 TCTAGA T^CTAGA
XhoI 3341 CTCGAG C^TCGAG
XmnI 3406 GAATCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccI 891 GTCTAC GT^[AC][GT]AC
3689 GTATAC
AgeI 887 ACCGGT A^CCGGT
2938 ACCGGT
AseI 922 ATTAAT AT^TAAT
2196 ATTAAT
AsnI 922 ATTAAT AT^TAAT
2196 ATTAAT
BbrPI 1907 CACGTG CAC^GTG
3114 CACGTG
BciVI 2304 GGATAC GTATCC(6/5)
3528 GTATCC
BglII 3849 AGATCT A^GATCT
3958 AGATCT
BlpI 2232 GCTAAGC GC^T[ACGT]AGC
3500 GCTTAGC
Bpu1102I 2232 GCTAAGC GC^T[ACGT]AGC
3500 GCTTAGC
BsaAI 1907 CACGTG [CT]AC^GT[GA]
3114 CACGTG
BsaI 4188 GAGACC GGTCTC(1/5)
3281 GGTCTC
BsgI 928 GTGCAG GTGCAG(16/14)
2733 GTGCAG
BsrDI 3201 CATTGC GCAATG(2/0)
4043 CATTGC
BsrFI 887 ACCGGT [GA]^CCGG[CT]
2938 ACCGGT
CelII 2232 GCTAAGC GC^T[ACGT]AGC
3500 GCTTAGC
Cfr10I 887 ACCGGT [GA]^CCGG[CT]
2938 ACCGGT
DsaI 587 CCATGG C^C[GA][CT]GG
4086 CCATGG
EcoRI 870 GAATTC G^AATTC
1637 GAATTC
EcoT22I 480 ATGCAT ATGCA^T
1310 ATGCAT
HindIII 392 AAGCTT A^AGCTT
2235 AAGCTT
MspA1I 3133 CAGCGG C[AC]G^C[GT]G
4162 CAGCTG
NcoI 587 CCATGG C^CATGG
4086 CCATGG
NsiI 480 ATGCAT ATGCA^T
1310 ATGCAT
PinAI 887 ACCGGT A^CCGGT
2938 ACCGGT
PmaCI 1907 CACGTG CAC^GTG
3114 CACGTG
PmlI 1907 CACGTG CAC^GTG
3114 CACGTG
PshBI 922 ATTAAT AT^TAAT
2196 ATTAAT
PstI 1247 CTGCAG CTGCA^G
4327 CTGCAG
SpeI 771 ACTAGT A^CTAGT
3880 ACTAGT
SspI 295 AATATT AAT^ATT
1186 AATATT
StuI 284 AGGCCT AGG^CCT
4280 AGGCCT
TaqI 3143 TCGA T^CGA
3342 TCGA
TthHB8I 3143 TCGA T^CGA
3342 TCGA
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# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AspHI AvaII
BalI BbsI BbvI BfaI BmyI
BpmI BpuAI BsaJI BsaWI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrI BssKI BstAPI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraI EaeI EarI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MfeI MflI
MluNI MnlI MscI MseI MslI
MspI MunI MvaI MwoI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XcmI XhoII