Restriction map for
pFN21AE2480
(Linear) (Six-base) MAPSORT of: KIEE2480.seq Check: 6234 from: 1 to: 4359
KIEE2480.orf
With 182 enzymes: SgfI *
August 27, 2013 13:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 3631 4359
Size: 3631 728
AccI GT'mk_AC
Cuts at: 0 892 3690 4359
Size: 892 2798 669
Fragments arranged by size:
2798 892 669
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 128 580 743 1117 1694 2395 2578 2659
Size: 128 452 163 374 577 701 183 81
Cuts at: 2659 3122 3397 3644 4359
Size: 463 275 247 715
Fragments arranged by size:
715 701 577 463 452 374 275 247
183 163 128 81
AfeI AGC'GCT
Cuts at: 0 423 4359
Size: 423 3936
AflIII A'CryG_T
Cuts at: 0 1906 1936 2163 3113 3115 4359
Size: 1906 30 227 950 2 1244
Fragments arranged by size:
1906 1244 950 227 30 2
AgeI A'CCGG_T
Cuts at: 0 887 2938 4359
Size: 887 2051 1421
Fragments arranged by size:
2051 1421 887
AhdI GACnn_n'nnGTC
Cuts at: 0 1544 4359
Size: 1544 2815
AlwNI CAG_nnn'CTG
Cuts at: 0 1441 1692 2818 4326 4359
Size: 1441 251 1126 1508 33
Fragments arranged by size:
1508 1441 1126 251 33
ApoI r'AATT_y
Cuts at: 0 323 638 870 985 1090 1637 2700 3053
Size: 323 315 232 115 105 547 1063 353
Cuts at: 3053 3512 3862 3988 4359
Size: 459 350 126 371
Fragments arranged by size:
1063 547 459 371 353 350 323 315
232 126 115 105
AseI AT'TA_AT
Cuts at: 0 923 2197 4359
Size: 923 1274 2162
Fragments arranged by size:
2162 1274 923
AvaI C'yCGr_G
Cuts at: 0 3341 4359
Size: 3341 1018
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2297 2330 4359
Size: 2297 33 2029
Fragments arranged by size:
2297 2029 33
BanI G'GyrC_C
Cuts at: 0 884 4359
Size: 884 3475
BanII G_rGCy'C
Cuts at: 0 3342 4359
Size: 3342 1017
BbsI GAAGACnn'nnnn_
Cuts at: 0 871 2341 3758 4359
Size: 871 1470 1417 601
Fragments arranged by size:
1470 1417 871 601
BciVI GTATCCnnnnn_n'
Cuts at: 0 2298 3539 4359
Size: 2298 1241 820
Fragments arranged by size:
2298 1241 820
BclI T'GATC_A
Cuts at: 0 2 4359
Size: 2 4357
BfrBI ATG'CAT
Cuts at: 0 482 1312 4359
Size: 482 830 3047
Fragments arranged by size:
3047 830 482
BglII A'GATC_T
Cuts at: 0 3849 3958 4359
Size: 3849 109 401
Fragments arranged by size:
3849 401 109
BlpI GC'TnA_GC
Cuts at: 0 2233 3501 4359
Size: 2233 1268 858
Fragments arranged by size:
2233 1268 858
Bme1580I G_kGCm'C
Cuts at: 0 1914 4359
Size: 1914 2445
BmgBI CAC'GTC
Cuts at: 0 1939 4359
Size: 1939 2420
BmrI ACTGGGnnnn_n'
Cuts at: 0 1005 2996 4359
Size: 1005 1991 1363
Fragments arranged by size:
1991 1363 1005
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 517 1463 1940 3559 3718 4000 4027 4359
Size: 517 946 477 1619 159 282 27 332
Fragments arranged by size:
1619 946 517 477 332 282 159 27
Bpu10I CC'TnA_GC
Cuts at: 0 4114 4359
Size: 4114 245
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2259 2617 3292 4359
Size: 2259 358 675 1067
Fragments arranged by size:
2259 1067 675 358
BsaI GGTCTCn'nnnn_
Cuts at: 0 3287 4182 4359
Size: 3287 895 177
Fragments arranged by size:
3287 895 177
BsaAI yAC'GTr
Cuts at: 0 1909 3116 4359
Size: 1909 1207 1243
Fragments arranged by size:
1909 1243 1207
BsaHI Gr'CG_yC
Cuts at: 0 4287 4359
Size: 4287 72
BsaWI w'CCGG_w
Cuts at: 0 887 2938 3021 4024 4359
Size: 887 2051 83 1003 335
Fragments arranged by size:
2051 1003 887 335 83
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 293 1173 1570 4359
Size: 293 880 397 2789
Fragments arranged by size:
2789 880 397 293
BseYI C'CCAG_C
Cuts at: 0 424 4359
Size: 424 3935
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 949 2754 4359
Size: 949 1805 1605
Fragments arranged by size:
1805 1605 949
BsiHKAI G_wGCw'C
Cuts at: 0 1731 3342 3616 4182 4359
Size: 1731 1611 274 566 177
Fragments arranged by size:
1731 1611 566 274 177
BsmI GAATG_Cn'
Cuts at: 0 1016 4359
Size: 1016 3343
BsmBI CGTCTCn'nnnn_
Cuts at: 0 4294 4359
Size: 4294 65
Bsp1286I G_dGCh'C
Cuts at: 0 1731 1914 3342 3616 4182 4359
Size: 1731 183 1428 274 566 177
Fragments arranged by size:
1731 1428 566 274 183 177
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 3558 3631 3659 4359
Size: 3558 73 28 700
Fragments arranged by size:
3558 700 73 28
BsrDI GCAATG_nn'
Cuts at: 0 3200 4042 4359
Size: 3200 842 317
Fragments arranged by size:
3200 842 317
BsrFI r'CCGG_y
Cuts at: 0 887 2938 4359
Size: 887 2051 1421
Fragments arranged by size:
2051 1421 887
BsrGI T'GTAC_A
Cuts at: 0 1319 4359
Size: 1319 3040
BssSI C'ACGA_G
Cuts at: 0 3655 4359
Size: 3655 704
BstAPI GCAn_nnn'nTGC
Cuts at: 0 298 2732 4326 4338 4359
Size: 298 2434 1594 12 21
Fragments arranged by size:
2434 1594 298 21 12
BstEII G'GTnAC_C
Cuts at: 0 3040 4359
Size: 3040 1319
BstXI CCAn_nnnn'nTGG
Cuts at: 0 318 4359
Size: 318 4041
BstYI r'GATC_y
Cuts at: 0 1569 2254 3849 3958 4359
Size: 1569 685 1595 109 401
Fragments arranged by size:
1595 1569 685 401 109
BstZ17I GTA'TAC
Cuts at: 0 3691 4359
Size: 3691 668
BtgI C'CryG_G
Cuts at: 0 587 4086 4359
Size: 587 3499 273
Fragments arranged by size:
3499 587 273
BtsI GCAGTG_nn'
Cuts at: 0 937 2742 3489 4359
Size: 937 1805 747 870
Fragments arranged by size:
1805 937 870 747
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 101 136 4359
Size: 101 35 4223
Fragments arranged by size:
4223 101 35
DraI TTT'AAA
Cuts at: 0 684 1043 1662 1881 4359
Size: 684 359 619 219 2478
Fragments arranged by size:
2478 684 619 359 219
EaeI y'GGCC_r
Cuts at: 0 50 308 3486 4359
Size: 50 258 3178 873
Fragments arranged by size:
3178 873 258 50
EarI CTCTTCn'nnn_
Cuts at: 0 1136 3098 3620 4359
Size: 1136 1962 522 739
Fragments arranged by size:
1962 1136 739 522
EcoICRI GAG'CTC
Cuts at: 0 3340 4359
Size: 3340 1019
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 128 517 580 743 1117 1463 1694 1940
Size: 128 389 63 163 374 346 231 246
Cuts at: 1940 2395 2578 2659 3122 3397 3559 3644 3718
Size: 455 183 81 463 275 162 85 74
Cuts at: 3718 4000 4027 4359
Size: 282 27 332
Fragments arranged by size:
463 455 389 374 346 332 282 275
246 231 183 163 162 128 85 81
74 63 27
EcoRI G'AATT_C
Cuts at: 0 870 1637 4359
Size: 870 767 2722
Fragments arranged by size:
2722 870 767
EcoRV GAT'ATC
Cuts at: 0 2894 4359
Size: 2894 1465
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2253 2406 4359
Size: 2253 153 1953
Fragments arranged by size:
2253 1953 153
HaeII r_GCGC'y
Cuts at: 0 425 4359
Size: 425 3934
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1530 1562 2529 2561 2590 2622 2664 2696
Size: 1530 32 967 32 29 32 42 32
Cuts at: 2696 2973 3005 3265 3297 4359
Size: 277 32 260 32 1062
Fragments arranged by size:
1530 1062 967 277 260 42 32 32
32 32 32 32 29
HincII GTy'rAC
Cuts at: 0 2455 2744 3174 3298 4359
Size: 2455 289 430 124 1061
Fragments arranged by size:
2455 1061 430 289 124
HindIII A'AGCT_T
Cuts at: 0 392 2235 4359
Size: 392 1843 2124
Fragments arranged by size:
2124 1843 392
HpaI GTT'AAC
Cuts at: 0 2744 4359
Size: 2744 1615
MfeI C'AATT_G
Cuts at: 0 1424 1764 2002 4359
Size: 1424 340 238 2357
Fragments arranged by size:
2357 1424 340 238
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 3730 4359
Size: 3730 629
MscI TGG'CCA
Cuts at: 0 52 310 3488 4359
Size: 52 258 3178 871
Fragments arranged by size:
3178 871 258 52
MslI CAynn'nnrTG
Cuts at: 0 2422 3143 4173 4359
Size: 2422 721 1030 186
Fragments arranged by size:
2422 1030 721 186
MspA1I CmG'CkG
Cuts at: 0 3135 4164 4359
Size: 3135 1029 195
Fragments arranged by size:
3135 1029 195
NcoI C'CATG_G
Cuts at: 0 587 4086 4359
Size: 587 3499 273
Fragments arranged by size:
3499 587 273
NsiI A_TGCA'T
Cuts at: 0 484 1314 4359
Size: 484 830 3045
Fragments arranged by size:
3045 830 484
NspI r_CATG'y
Cuts at: 0 1181 4359
Size: 1181 3178
PasI CC'CwG_GG
Cuts at: 0 3153 4359
Size: 3153 1206
PflMI CCAn_nnn'nTGG
Cuts at: 0 847 4359
Size: 847 3512
PfoI T'CCnGG_A
Cuts at: 0 1169 1472 4359
Size: 1169 303 2887
Fragments arranged by size:
2887 1169 303
PmlI CAC'GTG
Cuts at: 0 1909 3116 4359
Size: 1909 1207 1243
Fragments arranged by size:
1909 1243 1207
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 74 106 4359
Size: 74 32 4253
Fragments arranged by size:
4253 74 32
PsiI TTA'TAA
Cuts at: 0 1825 4359
Size: 1825 2534
PspXI vC'TCGA_Gb
Cuts at: 0 3341 4359
Size: 3341 1018
PstI C_TGCA'G
Cuts at: 0 1251 4331 4359
Size: 1251 3080 28
Fragments arranged by size:
3080 1251 28
PvuII CAG'CTG
Cuts at: 0 4164 4359
Size: 4164 195
SacI G_AGCT'C
Cuts at: 0 3342 4359
Size: 3342 1017
ScaI AGT'ACT
Cuts at: 0 1593 4359
Size: 1593 2766
SexAI A'CCwGG_T
Cuts at: 0 1896 4359
Size: 1896 2463
SfcI C'TryA_G
Cuts at: 0 1247 2060 4327 4359
Size: 1247 813 2267 32
Fragments arranged by size:
2267 1247 813 32
SmlI C'TyrA_G
Cuts at: 0 2238 2596 3271 3341 4359
Size: 2238 358 675 70 1018
Fragments arranged by size:
2238 1018 675 358 70
SpeI A'CTAG_T
Cuts at: 0 771 3880 4359
Size: 771 3109 479
Fragments arranged by size:
3109 771 479
SspI AAT'ATT
Cuts at: 0 297 1188 4359
Size: 297 891 3171
Fragments arranged by size:
3171 891 297
StuI AGG'CCT
Cuts at: 0 286 4282 4359
Size: 286 3996 77
Fragments arranged by size:
3996 286 77
StyI C'CwwG_G
Cuts at: 0 62 259 287 587 2498 4035 4086 4359
Size: 62 197 28 300 1911 1537 51 273
Fragments arranged by size:
1911 1537 300 273 197 62 51 28
TatI w'GTAC_w
Cuts at: 0 24 1319 1591 1719 1836 3232 3267 3287
Size: 24 1295 272 128 117 1396 35 20
Cuts at: 3287 4359
Size: 1072
Fragments arranged by size:
1396 1295 1072 272 128 117 35 24
20
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 171 203 4040 4072 4359
Size: 171 32 3837 32 287
Fragments arranged by size:
3837 287 171 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1898 4359
Size: 1898 2461
XbaI T'CTAG_A
Cuts at: 0 1572 4359
Size: 1572 2787
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 60 1921 3978 4084 4359
Size: 60 1861 2057 106 275
Fragments arranged by size:
2057 1861 275 106 60
XhoI C'TCGA_G
Cuts at: 0 3341 4359
Size: 3341 1018
XmnI GAAnn'nnTTC
Cuts at: 0 3410 4359
Size: 3410 949
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AgeI AhdI AlwNI
ApoI AseI AvaI BaeI BanI BanII BbsI BciVI
BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BseRI BseYI
BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrDI BsrFI
BsrGI BssSI BstAPI BstEII BstXI BstYI BstZ17I BtgI
BtsI CspCI DraI EaeI EarI EcoICRI Eco57MI EcoRI
EcoRV FalI HaeII Hin4I HincII HindIII HpaI MfeI
MmeI MscI MslI MspA1I NcoI NsiI NspI PasI
PflMI PfoI PmlI PpiI PsiI PspXI PstI PvuII
SacI ScaI SexAI SfcI SmlI SpeI SspI StuI
StyI TatI TstI Tth111I XbaI XcmI XhoI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AleI AlfI AloI ApaI
ApaLI AscI AsiSI AvrII BamHI BbeI BbvCI BcgI
BglI BmtI BplI BsaBI BsaXI BsiEI BsiWI BspEI
BspHI BsrBI BssHII BstBI Bsu36I BtgZI ClaI DraIII
DrdI EagI EciI EcoNI EcoO109I FseI FspI FspAI
KasI KpnI MluI NaeI NarI NdeI NgoMIV NheI
NotI NruI PacI PciI PmeI PpuMI PshAI PspOMI
PsrI PvuI RsrII SacII SalI SanDI SapI SbfI
SfiI SfoI SgfI SgrAI SmaI SnaBI SphI SrfI
SwaI TaqII XmaI ZraI