################################## # NO CUT ENZYMES ( 116 enzymes) # ################################## Acc65I AccI AclI AflII AflIII AgeI AhdI AocI Aor51HI ApaI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BanII BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsiEI BsiWI BsmBI Bsp1407I BspHI BspLU11I BsrBI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I CelII CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoT22I FbaI FseI FspI HincII HindII HpaI KasI KpnI KspI MfeI MluI MseI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SwaI Tru9I Tth111I XbaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AatII 1462 GACGTC GACGT^C AccIII 37 TCCGGA T^CCGGA AcyI 1462 GACGTC G[GA]^CG[CT]C Alw44I 1046 GTGCAC G^TGCAC ApaLI 1046 GTGCAC G^TGCAC AspHI 1046 GTGCAC G[AT]GC[AT]^C AvaII 35 GGTCC G^G[AT]CC BamHI 28 GGATCC G^GATCC BanI 1472 GGCACC G^G[CT][GA]CC BbsI 802 GTCTTC GAAGAC(2/6) BpmI 654 CTGGAG CTGGAG(16/14) BpuAI 802 GTCTTC GAAGAC(2/6) BsaBI 115 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 1462 GACGTC G[GA]^CG[CT]C BsaI 41 GAGACC GGTCTC(1/5) BsaWI 37 TCCGGA [AT]^CCGG[AT] BseAI 37 TCCGGA T^CCGGA BsiHKAI 1046 GTGCAC G[AT]GC[AT]^C BsmI 930 GAATGC GAATGC(1/-1) BspDI 266 ATCGAT AT^CGAT BspEI 37 TCCGGA T^CCGGA BspMI 1517 ACCTGC ACCTGC(4/8) BsrDI 810 CATTGC GCAATG(2/0) BstAPI 1227 GCAGCAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC ClaI 266 ATCGAT AT^CGAT DsaI 1299 CCACGG C^C[GA][CT]GG EcoRI 313 GAATTC G^AATTC EcoT14I 447 CCTTGG C^C[AT][AT]GG HaeII 1588 AGCGCC [GA]GCGC^[CT] Hin1I 1462 GACGTC G[GA]^CG[CT]C MaeII 1463 ACGT A^CGT MaeIII 1460 GTGAC ^GT[ACGT]AC MamI 115 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 37 TCCGGA T^CCGGA NspI 1562 ACATGC [GA]CATG^[CT] PaeR7I 461 CTCGAG C^TCGAG PflMI 129 CCAGGTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PvuII 355 CAGCTG CAG^CTG SmaI 302 CCCGGG CCC^GGG SmlI 461 CTCGAG C^T[CT][GA]AG SspI 555 AATATT AAT^ATT StuI 1448 AGGCCT AGG^CCT StyI 447 CCTTGG C^C[AT][AT]GG TfiI 1570 GAATC G^A[AT]TC Tsp45I 1460 GTGAC ^GT[GC]AC Van91I 129 CCAGGTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 461 CTCGAG C^TCGAG XmaCI 302 CCCGGG C^CCGGG XmaI 302 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AccII 8 CGCG CG^CG 643 CGCG Asp700I 519 GAAGGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 690 GAACCACTTC AvaI 302 CCCGGG C^[CT]CG[GA]G 461 CTCGAG BfaI 707 CTAG C^TAG 841 CTAG BglI 602 GCCGATATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1166 GCCGGGAAGGC BsoBI 302 CCCGGG C^[CT]CG[GA]G 461 CTCGAG BsrFI 890 ACCGGC [GA]^CCGG[CT] 1328 GCCGGT BstUI 8 CGCG CG^CG 643 CGCG BstXI 485 CCAATGATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1034 CCAAGCCTGTGG CfoI 894 GCGC GCG^C 1589 GCGC Cfr10I 890 ACCGGC [GA]^CCGG[CT] 1328 GCCGGT DraII 209 AGGCCCT [GA]G^G[ACGT]CC[CT] 419 GGGGCCT DraIII 1300 CACGGAGTG CAC[ACGT][ACGT][ACGT]^GTG 1420 CACATTGTG EcoO109I 209 AGGCCCT [GA]G^G[ACGT]CC[CT] 419 GGGGCCT EcoRV 468 GATATC GAT^ATC 1147 GATATC HhaI 894 GCGC GCG^C 1589 GCGC HinP1I 894 GCGC G^CGC 1589 GCGC HindIII 438 AAGCTT A^AGCTT 1181 AAGCTT MaeI 707 CTAG C^TAG 841 CTAG MslI 916 CATGAGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1060 CACCAGGATG MspA1I 355 CAGCTG C[AC]G^C[GT]G 1323 CCGCTG MvnI 8 CGCG CG^CG 643 CGCG ScaI 837 AGTACT AGT^ACT 1547 AGTACT SfcI 537 CTACAG C^T[GA][CT]AG 1368 CTACAG XcmI 347 CCACAGGGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1382 CCACTGGCAAAGTGG XmnI 519 GAAGGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 690 GAACCACTTC ######################################### # 3 OR MORE CUT ENZYMES ( 67 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI BalI BbvI BcnI BmyI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII EaeI EarI EcoRII FauI Fnu4HI FokI HaeIII HapII HgaI HinfI HpaII HphI ItaI Ksp632I MboI MboII MflI MluNI MnlI MscI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI TaqI TseI Tsp509I TspRI TthHB8I XhoII