Restriction map for
pFN21AE3234
(Circular) (Six-base) MAPSORT of: pFN21AE3234.seq Check: 6060 from: 1 to: 6343
pFN21AE3234 6343 bp
With 182 enzymes: SgfI *
December 28, 2011 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3471 4153 278
Size: 53 83 186 936 1935 682 2468
Fragments arranged by size:
2468 1935 936 682 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3686 1937
Size: 1749 4594
Acc65I G'GTAC_C
Cuts at: 3664 3664
Size: 6343
AclI AA'CG_TT
Cuts at: 1563 4470 4843 1563
Size: 2907 373 3063
Fragments arranged by size:
3063 2907 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2891 3284 4406 5790 6058 1632
Size: 1259 393 1122 1384 268 1917
Fragments arranged by size:
1917 1384 1259 1122 393 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6146
AflIII A'CryG_T
Cuts at: 1203 5242 6219 1203
Size: 4039 977 1327
Fragments arranged by size:
4039 1327 977
AgeI A'CCGG_T
Cuts at: 5174 5174
Size: 6343
AhdI GACnn_n'nnGTC
Cuts at: 5072 5072
Size: 6343
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6343
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6343
AlwNI CAG_nnn'CTG
Cuts at: 2140 3381 3578 4086 5658 2140
Size: 1241 197 508 1572 2825
Fragments arranged by size:
2825 1572 1241 508 197
ApaLI G'TGCA_C
Cuts at: 3051 4400 5556 3051
Size: 1349 1156 3838
Fragments arranged by size:
3838 1349 1156
ApoI r'AATT_y
Cuts at: 1493 2197 2318 2627 3652 3878 4030 6139 1493
Size: 704 121 309 1025 226 152 2109 1697
Fragments arranged by size:
2109 1697 1025 704 309 226 152 121
AseI AT'TA_AT
Cuts at: 160 1032 4897 160
Size: 872 3865 1606
Fragments arranged by size:
3865 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6343
AvaI C'yCGr_G
Cuts at: 1870 1943 2307 2466 3668 1870
Size: 73 364 159 1202 4545
Fragments arranged by size:
4545 1202 364 159 73
BamHI G'GATC_C
Cuts at: 2033 3673 4040 2033
Size: 1640 367 4336
Fragments arranged by size:
4336 1640 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3477 3664 5119 618
Size: 325 217 627 1690 187 1455 1842
Fragments arranged by size:
1842 1690 1455 627 325 217 187
BanII G_rGCy'C
Cuts at: 1391 3662 1391
Size: 2271 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2800 928
Size: 971 901 4471
Fragments arranged by size:
4471 971 901
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4534 4568 1903
Size: 34 2597 34 3678
Fragments arranged by size:
3678 2597 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4235 5445 4235
Size: 1210 5133
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6343
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2613 3177 4954
Size: 107 122 71 631 605 941 564 1777
Cuts at: 4954 136
Size: 1525
Fragments arranged by size:
1777 1525 941 631 605 564 122 107
71
BglII A'GATC_T
Cuts at: 6338 6338
Size: 6343
BlpI GC'TnA_GC
Cuts at: 3760 3760
Size: 6343
Bme1580I G_kGCm'C
Cuts at: 1790 3055 3243 3612 4404 5560 1790
Size: 1265 188 369 792 1156 2573
Fragments arranged by size:
2573 1265 1156 792 369 188
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6343
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3226 3599 4074
Size: 416 53 83 399 24 1809 373 475
Cuts at: 4074 5032 442
Size: 958 1753
Fragments arranged by size:
1809 1753 958 475 416 399 373 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6343
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2680 5003 1239
Size: 1441 2323 2579
Fragments arranged by size:
2579 2323 1441
Bpu10I CC'TnA_GC
Cuts at: 2369 2369
Size: 6343
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4468 5333 5631 4468
Size: 865 298 5180
Fragments arranged by size:
5180 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2040 5006 882
Size: 617 541 2966 2219
Fragments arranged by size:
2966 2219 617 541
BsaAI yAC'GTr
Cuts at: 493 1204 4134 493
Size: 711 2930 2702
Fragments arranged by size:
2930 2702 711
BsaBI GATnn'nnATC
Cuts at: 2124 6337 2124
Size: 4213 2130
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3468 4150 4532 5167
Size: 53 83 186 936 1935 682 382 635
Cuts at: 5167 275
Size: 1451
Fragments arranged by size:
1935 1451 936 682 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2042 4776 5174 5448 5595 6006 2042
Size: 2734 398 274 147 411 2379
Fragments arranged by size:
2734 2379 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6118
Fragments arranged by size:
6118 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2141 2199 2815 1186
Size: 955 58 616 4714
Fragments arranged by size:
4714 955 616 58
BseYI C'CCAG_C
Cuts at: 2362 5546 6042 2362
Size: 3184 496 2663
Fragments arranged by size:
3184 2663 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2049 2198 2211 3299 1163
Size: 573 313 149 13 1088 4207
Fragments arranged by size:
4207 1088 573 313 149 13
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3793 4554 5582 1540
Size: 275 186 1792 761 1028 2301
Fragments arranged by size:
2301 1792 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3055 4404 4489 5560 3055
Size: 1349 85 1071 3838
Fragments arranged by size:
3838 1349 1071 85
BsmI GAATG_Cn'
Cuts at: 2941 3854 3947 2941
Size: 913 93 5337
Fragments arranged by size:
5337 913 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3055 3243 3612 3662 4404 4489 5560
Size: 399 1265 188 369 50 742 85 1071
Cuts at: 5560 1391
Size: 2174
Fragments arranged by size:
2174 1265 1071 742 399 369 188 85
50
BspEI T'CCGG_A
Cuts at: 2042 6006 2042
Size: 3964 2379
BspHI T'CATG_A
Cuts at: 4232 5962 4232
Size: 1730 4613
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3531 3698 844
Size: 2687 167 3489
Fragments arranged by size:
3489 2687 167
BsrBI CCG'CTC
Cuts at: 1394 1823 4230 1394
Size: 429 2407 3507
Fragments arranged by size:
3507 2407 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2814 4838 5012 65
Size: 1183 1566 2024 174 1396
Fragments arranged by size:
2024 1566 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2895 3333 4987 5174 1700
Size: 110 1085 438 1654 187 2869
Fragments arranged by size:
2869 1654 1085 438 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6343
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6343
BssSI C'ACGA_G
Cuts at: 4403 5415 4403
Size: 1012 5331
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3238 794
Size: 2444 3899
BstBI TT'CG_AA
Cuts at: 5147 5147
Size: 6343
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6343
BstXI CCAn_nnnn'nTGG
Cuts at: 2497 3046 2497
Size: 549 5794
BstYI r'GATC_y
Cuts at: 1972 2033 2125 2776 3673 4040 4425 4442 5894
Size: 61 92 651 897 367 385 17 1452
Cuts at: 5894 5980 5992 6338 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 897 651 385 367 346 92
86 61 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3304 513
Size: 552 939 1300 3552
Fragments arranged by size:
3552 1300 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2664 487
Size: 683 56 1438 4166
Fragments arranged by size:
4166 1438 683 56
BtsI GCAGTG_nn'
Cuts at: 2032 2766 2946 3859 4653 4673 2032
Size: 734 180 913 794 20 3702
Fragments arranged by size:
3702 913 794 734 180 20
ClaI AT'CG_AT
Cuts at: 1554 2272 1554
Size: 718 5625
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6308
DraI TTT'AAA
Cuts at: 3647 3647
Size: 6343
DraIII CAC_nnn'GTG
Cuts at: 3310 3430 3310
Size: 120 6223
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5350 809
Size: 507 4034 1802
Fragments arranged by size:
4034 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2349 2614 3358
Size: 54 1425 50 275 18 519 265 744
Cuts at: 3358 3421 3790 4679 6308 8
Size: 63 369 889 1629 43
Fragments arranged by size:
1629 1425 889 744 519 369 275 265
63 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3790 1537
Size: 275 1978 4090
Fragments arranged by size:
4090 1978 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2065 2094 2103 2229 3483 4273 1353
Size: 712 29 9 126 1254 790 3423
Fragments arranged by size:
3423 1254 790 712 126 29 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3271 4930 5304 5450 387
Size: 1064 138 1682 1659 374 146 1280
Fragments arranged by size:
1682 1659 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2680 2891 3284 4406 5003 5790 6058
Size: 393 1048 211 393 1122 597 787 268
Cuts at: 6058 1239
Size: 1524
Fragments arranged by size:
1524 1122 1048 787 597 393 393 268
211
EcoO109I rG'GnC_Cy
Cuts at: 2215 2425 2215
Size: 210 6133
EcoRI G'AATT_C
Cuts at: 2318 3652 4030 2318
Size: 1334 378 4631
Fragments arranged by size:
4631 1334 378
EcoRV GAT'ATC
Cuts at: 2475 3154 2475
Size: 679 5664
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2410 2410
Size: 6343
FspI TGC'GCA
Cuts at: 4849 4849
Size: 6343
HaeII r_GCGC'y
Cuts at: 1678 3597 5490 1678
Size: 1919 1893 2531
Fragments arranged by size:
2531 1919 1893
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2501 2533 2945 2977 4984
Size: 32 135 32 929 32 412 32 2007
Cuts at: 4984 5016 5058 5090 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2007 929 412 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3687 3933 669
Size: 988 281 1749 246 3079
Fragments arranged by size:
3079 1749 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2443 3186 748
Size: 1695 743 3905
Fragments arranged by size:
3905 1695 743
HpaI GTT'AAC
Cuts at: 3933 3933
Size: 6343
KpnI G_GTAC'C
Cuts at: 3668 3668
Size: 6343
MfeI C'AATT_G
Cuts at: 3942 3942
Size: 6343
MluI A'CGCG_T
Cuts at: 6219 6219
Size: 6343
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2069 2617 3144 3181 5457 5641 1305
Size: 764 548 527 37 2276 184 2007
Fragments arranged by size:
2276 2007 764 548 527 184 37
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2351 3360 3423 10
Size: 54 1425 343 519 1009 63 2930
Fragments arranged by size:
2930 1425 1009 519 343 63 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2925 3069 4301 4660 4819 518
Size: 648 1759 144 1232 359 159 2042
Fragments arranged by size:
2042 1759 1232 648 359 159 144
MspA1I CmG'CkG
Cuts at: 1606 2362 3330 3759 4436 5584 5829 1606
Size: 756 968 429 677 1148 245 2120
Fragments arranged by size:
2120 1148 968 756 677 429 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4852
Fragments arranged by size:
4852 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6343
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6343
NotI GC'GGCC_GC
Cuts at: 3790 3790
Size: 6343
NspI r_CATG'y
Cuts at: 1132 1229 3571 3701 5246 1132
Size: 97 2342 130 1545 2229
Fragments arranged by size:
2342 2229 1545 130 97
PasI CC'CwG_GG
Cuts at: 1348 3272 1348
Size: 1924 4419
PciI A'CATG_T
Cuts at: 5242 5242
Size: 6343
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2140 1108
Size: 1032 5311
PfoI T'CCnGG_A
Cuts at: 3558 3558
Size: 6343
PmeI GTTT'AAAC
Cuts at: 3647 3647
Size: 6343
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2412 2444 4412 4444 5925 5957 2412
Size: 32 1968 32 1481 32 2798
Fragments arranged by size:
2798 1968 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6343
PsiI TTA'TAA
Cuts at: 3913 3913
Size: 6343
PstI C_TGCA'G
Cuts at: 830 3695 830
Size: 2865 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6343
PvuII CAG'CTG
Cuts at: 2362 2362
Size: 6343
SalI G'TCGA_C
Cuts at: 1936 3685 1936
Size: 1749 4594
SbfI CC_TGCA'GG
Cuts at: 3695 3695
Size: 6343
ScaI AGT'ACT
Cuts at: 2844 3554 4591 6303 2844
Size: 710 1037 1712 2884
Fragments arranged by size:
2884 1712 1037 710
SfcI C'TryA_G
Cuts at: 826 1045 2542 3373 3691 4826 5507 5698 826
Size: 219 1497 831 318 1135 681 191 1471
Fragments arranged by size:
1497 1471 1135 831 681 318 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6343
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6343
SmaI CCC'GGG
Cuts at: 1872 2309 3670 1872
Size: 437 1361 4545
Fragments arranged by size:
4545 1361 437
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2466 4447 5348 5610 820
Size: 197 926 523 1981 901 262 1553
Fragments arranged by size:
1981 1553 926 901 523 262 197
SnaBI TAC'GTA
Cuts at: 493 4134 493
Size: 3641 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6343
SphI G_CATG'C
Cuts at: 1132 3701 1132
Size: 2569 3774
SspI AAT'ATT
Cuts at: 5 52 2562 4267 5
Size: 47 2510 1705 2081
Fragments arranged by size:
2510 2081 1705 47
StuI AGG'CCT
Cuts at: 1517 3455 1517
Size: 1938 4405
StyI C'CwwG_G
Cuts at: 513 1065 2004 2452 3782 513
Size: 552 939 448 1330 3074
Fragments arranged by size:
3074 1330 939 552 448
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2898 3038 4370 4387 4540 4725 5170
Size: 1255 1318 140 1332 17 153 185 445
Cuts at: 5170 325
Size: 1498
Fragments arranged by size:
1498 1332 1318 1255 445 185 153 140
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2842 3552 4589
Size: 274 80 33 51 1443 865 710 1037
Cuts at: 4589 6301 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1037 865 710 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3212 3244 6303 6335 3212
Size: 32 3059 32 3220
Fragments arranged by size:
3220 3059 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6343
XbaI T'CTAG_A
Cuts at: 3679 3679
Size: 6343
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2359 3394 2359
Size: 1035 5308
XhoI C'TCGA_G
Cuts at: 1943 2466 1943
Size: 523 5820
XmaI C'CCGG_G
Cuts at: 1870 2307 3668 1870
Size: 437 1361 4545
Fragments arranged by size:
4545 1361 437
XmnI GAAnn'nnTTC
Cuts at: 2528 2699 4472 2528
Size: 171 1773 4399
Fragments arranged by size:
4399 1773 171
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3469 4151 276
Size: 53 83 186 936 1935 682 2468
Fragments arranged by size:
2468 1935 936 682 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI
EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI