(Linear) (Six-base) MAPSORT of: KIEE3234.seq Check: 6048 from: 1 to: 1638 KIEE3234.orf With 182 enzymes: SgfI * December 27, 2011 16:34 .. AatII G_ACGT'C Cuts at: 0 1466 1638 Size: 1466 172 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 886 1279 1638 Size: 886 393 359 Fragments arranged by size: 886 393 359 AlwNI CAG_nnn'CTG Cuts at: 0 135 1376 1573 1638 Size: 135 1241 197 65 Fragments arranged by size: 1241 197 135 65 ApaLI G'TGCA_C Cuts at: 0 1046 1638 Size: 1046 592 ApoI r'AATT_y Cuts at: 0 192 313 622 1638 Size: 192 121 309 1016 Fragments arranged by size: 1016 309 192 121 AvaI C'yCGr_G Cuts at: 0 302 461 1638 Size: 302 159 1177 Fragments arranged by size: 1177 302 159 BamHI G'GATC_C Cuts at: 0 28 1638 Size: 28 1610 BanI G'GyrC_C Cuts at: 0 1472 1638 Size: 1472 166 BbsI GAAGACnn'nnnn_ Cuts at: 0 795 1638 Size: 795 843 BglI GCCn_nnn'nGGC Cuts at: 0 608 1172 1638 Size: 608 564 466 Fragments arranged by size: 608 564 466 Bme1580I G_kGCm'C Cuts at: 0 1050 1238 1607 1638 Size: 1050 188 369 31 Fragments arranged by size: 1050 369 188 31 BmrI ACTGGGnnnn_n' Cuts at: 0 1221 1594 1638 Size: 1221 373 44 Fragments arranged by size: 1221 373 44 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 675 1638 Size: 675 963 Bpu10I CC'TnA_GC Cuts at: 0 364 1638 Size: 364 1274 BsaI GGTCTCn'nnnn_ Cuts at: 0 35 1638 Size: 35 1603 BsaBI GATnn'nnATC Cuts at: 0 119 1638 Size: 119 1519 BsaHI Gr'CG_yC Cuts at: 0 1463 1638 Size: 1463 175 BsaWI w'CCGG_w Cuts at: 0 37 1638 Size: 37 1601 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 136 194 810 1638 Size: 136 58 616 828 Fragments arranged by size: 828 616 136 58 BseYI C'CCAG_C Cuts at: 0 357 1638 Size: 357 1281 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 44 193 206 1294 1638 Size: 44 149 13 1088 344 Fragments arranged by size: 1088 344 149 44 13 BsiHKAI G_wGCw'C Cuts at: 0 1050 1638 Size: 1050 588 BsmI GAATG_Cn' Cuts at: 0 936 1638 Size: 936 702 Bsp1286I G_dGCh'C Cuts at: 0 1050 1238 1607 1638 Size: 1050 188 369 31 Fragments arranged by size: 1050 369 188 31 BspEI T'CCGG_A Cuts at: 0 37 1638 Size: 37 1601 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1526 1638 Size: 1526 112 BsrDI GCAATG_nn' Cuts at: 0 809 1638 Size: 809 829 BsrFI r'CCGG_y Cuts at: 0 890 1328 1638 Size: 890 438 310 Fragments arranged by size: 890 438 310 BstAPI GCAn_nnn'nTGC Cuts at: 0 1233 1638 Size: 1233 405 BstXI CCAn_nnnn'nTGG Cuts at: 0 492 1041 1638 Size: 492 549 597 Fragments arranged by size: 597 549 492 BstYI r'GATC_y Cuts at: 0 28 120 771 1638 Size: 28 92 651 867 Fragments arranged by size: 867 651 92 28 BtgI C'CryG_G Cuts at: 0 1299 1638 Size: 1299 339 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 659 1638 Size: 659 979 BtsI GCAGTG_nn' Cuts at: 0 27 761 941 1638 Size: 27 734 180 697 Fragments arranged by size: 734 697 180 27 ClaI AT'CG_AT Cuts at: 0 267 1638 Size: 267 1371 DraIII CAC_nnn'GTG Cuts at: 0 1305 1425 1638 Size: 1305 120 213 Fragments arranged by size: 1305 213 120 EaeI y'GGCC_r Cuts at: 0 344 609 1353 1416 1638 Size: 344 265 744 63 222 Fragments arranged by size: 744 344 265 222 63 EarI CTCTTCn'nnn_ Cuts at: 0 60 89 98 224 1478 1638 Size: 60 29 9 126 1254 160 Fragments arranged by size: 1254 160 126 60 29 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1266 1638 Size: 1266 372 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 675 886 1279 1638 Size: 675 211 393 359 Fragments arranged by size: 675 393 359 211 EcoO109I rG'GnC_Cy Cuts at: 0 210 420 1638 Size: 210 210 1218 Fragments arranged by size: 1218 210 210 EcoRI G'AATT_C Cuts at: 0 313 1638 Size: 313 1325 EcoRV GAT'ATC Cuts at: 0 470 1149 1638 Size: 470 679 489 Fragments arranged by size: 679 489 470 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 405 1638 Size: 405 1233 HaeII r_GCGC'y Cuts at: 0 1592 1638 Size: 1592 46 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 496 528 940 972 1638 Size: 496 32 412 32 666 Fragments arranged by size: 666 496 412 32 32 HindIII A'AGCT_T Cuts at: 0 438 1181 1638 Size: 438 743 457 Fragments arranged by size: 743 457 438 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 64 612 1139 1176 1638 Size: 64 548 527 37 462 Fragments arranged by size: 548 527 462 64 37 MscI TGG'CCA Cuts at: 0 346 1355 1418 1638 Size: 346 1009 63 220 Fragments arranged by size: 1009 346 220 63 MslI CAynn'nnrTG Cuts at: 0 920 1064 1638 Size: 920 144 574 Fragments arranged by size: 920 574 144 MspA1I CmG'CkG Cuts at: 0 357 1325 1638 Size: 357 968 313 Fragments arranged by size: 968 357 313 NspI r_CATG'y Cuts at: 0 1566 1638 Size: 1566 72 PasI CC'CwG_GG Cuts at: 0 1267 1638 Size: 1267 371 PflMI CCAn_nnn'nTGG Cuts at: 0 135 1638 Size: 135 1503 PfoI T'CCnGG_A Cuts at: 0 1553 1638 Size: 1553 85 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 407 439 1638 Size: 407 32 1199 Fragments arranged by size: 1199 407 32 PvuII CAG'CTG Cuts at: 0 357 1638 Size: 357 1281 ScaI AGT'ACT Cuts at: 0 839 1549 1638 Size: 839 710 89 Fragments arranged by size: 839 710 89 SfcI C'TryA_G Cuts at: 0 537 1368 1638 Size: 537 831 270 Fragments arranged by size: 831 537 270 SmaI CCC'GGG Cuts at: 0 304 1638 Size: 304 1334 SmlI C'TyrA_G Cuts at: 0 461 1638 Size: 461 1177 SspI AAT'ATT Cuts at: 0 557 1638 Size: 557 1081 StuI AGG'CCT Cuts at: 0 1450 1638 Size: 1450 188 StyI C'CwwG_G Cuts at: 0 447 1638 Size: 447 1191 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 893 1033 1638 Size: 893 140 605 Fragments arranged by size: 893 605 140 TatI w'GTAC_w Cuts at: 0 837 1547 1638 Size: 837 710 91 Fragments arranged by size: 837 710 91 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1207 1239 1638 Size: 1207 32 399 Fragments arranged by size: 1207 399 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 354 1389 1638 Size: 354 1035 249 Fragments arranged by size: 1035 354 249 XhoI C'TCGA_G Cuts at: 0 461 1638 Size: 461 1177 XmaI C'CCGG_G Cuts at: 0 302 1638 Size: 302 1336 XmnI GAAnn'nnTTC Cuts at: 0 523 694 1638 Size: 523 171 944 Fragments arranged by size: 944 523 171 ZraI GAC'GTC Cuts at: 0 1464 1638 Size: 1464 174 Enzymes that do cut: AatII AcuI AlwNI ApaLI ApoI AvaI BamHI BanI BbsI BglI Bme1580I BmrI BpmI Bpu10I BsaI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BspMI BsrDI BsrFI BstAPI BstXI BstYI BtgI BtgZI BtsI ClaI DraIII EaeI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI HaeII Hin4I HindIII MmeI MscI MslI MspA1I NspI PasI PflMI PfoI PpiI PvuII ScaI SfcI SmaI SmlI SspI StuI StyI TaqII TatI TstI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaI AscI AseI AsiSI AvrII BaeI BanII BbeI BbvCI BcgI BciVI BclI BfrBI BglII BlpI BmgBI BmtI BplI BpuEI BsaAI BsaXI BsiEI BsiWI BsmBI BspHI BsrBI BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I CspCI DraI DrdI EagI EcoICRI EcoNI FseI FspI FspAI HincII HpaI KasI KpnI MfeI MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PmeI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SwaI Tth111I XbaI